This study describes macroinvertebrate community structure and assesses its potential in detecting point and non-point sources of disturbance associated with rural and urban areas in the Upper Trinity River Basin. Geospatial techniques were used to quantify landuse within the watershed in a GIS. At rural sites near the headwaters of the Trinity River, collector-gathering burrowers that are adapted to minimal flow comprised the majority of taxa. Destinies of taxa compositions at downstream sites increased and shifted toward psammophilic and rheophilic invertebrates, including primarily collector-filtering clingers, that are characteristic of shifting sand habitats in large prairie rivers. Benthic community structure generally benefited from point source impacts including wastewater treatment plant effluents that maintained higher flow. Community indices were negatively associated with forest landuse and positively associated with urban landuse. Partial CCA determined that flow and landuse contributed equally to species dispersions. Comparisons with historical biomonitoring studies in upper Trinity River Basin indicate improved watershed health.

Effluent from on-site sewage treatment and disposal systems (OSTDS) is generally known to impact groundwaters and surface waters with nitrogen (N) and phosphorus (P) and other contaminants. Little research has quantified this problem along the Indian River Lagoon (IRL), especially in Indian River County (IRC) where there are 26,660 active systems. This study assessed the effects of OSTDS on contamination of surface and groundwaters along three urbanized canals and the St. Sebastian River in IRC, all of which flow into the Central IRL. Multiple lines of evidence were used to define the source of the nutrient loadings including the novel approach of using the artificial sweetener, sucralose, as an indicator of human sewage impact. Results indicate that areas with high densities of OSTDS are contributing N to surface waters and elevating N:P ratios through submarine groundwater discharge and promoting eutrophication in the Central IRL. / Includes bibliography. / Thesis (M.S.)--Florida Atlantic University, 2014. / FAU Electronic Theses and Dissertations Collection

M. Tech. (Department of Chemical Engineering, Faculty of Engineering and Technology), Vaal University of Technology / Fenton oxidation is classified into two processes, homogeneous and heterogeneous. Homogeneous Fenton oxidation process, have been shown to be efficient in the degradation of organic pollutants. However, it was shown to have limitations which can be addressed by the heterogeneous Fenton oxidation. Despite the high efficiency of the heterogeneous Fenton oxidation process in the degradation of recalcitrant organic pollutants, the currents synthesis trends of the heterogeneous Fenton catalyst have been proven to be time and energy constraining, since it involves the multi-step were the activated carbon have to be prepared first then co-precipitate the iron oxide on the activated carbon. However, as much as the heterogeneous Fenton catalyst has been proven to have high catalytic activity towards degradation of organic pollutants, these catalysts have some limitations, such limitations include metal ions being leached from the catalyst support into the treated water causing catalyst deactivation and a secondary pollution to the treated water. In this thesis, these catalysts have been applied in the degradation of recalcitrant organic pollutants such as methylene blue and phenols. This study focuses on the single step synthesis of iron oxide nanoparticles supported on activated carbon, were carbonaceous material is impregnated with iron salt then pyrolysed via microwave heating. Microwave power and the amount of iron salt were optimized. The prepared activated carbon-iron oxide composites were applied to the degradation of 2-nitrophenol (2-NP) and methylene blue (MB). Methylene blue was used as a model compound due to the fact that it is easier to monitor the degradation process with UV-Vis as compared to 2-nitrophenol . 2-nitrophenol the additional step for the adjustment of pH is required since nitrophenols are colorless in color at lower pH. The characterization showed that the microwave power and the amount of the iron precursor have an influence on the porosity and surface functional groups of the activated carbon. Further it was vi observed that microwave power and iron precursor influnces the amount of iron oxide formed on the surface of the support. It was also observed that the activated carbon-iron oxide composite have the catalytic effects on the Fenton oxidation process of MB and 2-NP. The parameters such as H2O2, pH, catalyst dose, initial concentration, temperature affect the degradation of both MB and 2-NP. Kinetics studies showed that Fenton is a surface driven reaction since the results fitted the pseudo first order model. The thermodynamics parameters also showed that the reaction is endothermic, spontaneous and is randomized. This implies that the reaction of the degradation of MB and 2-NP is feasible and the catalysts prepared have high catalytic activity. MB and 2-NP were degraded to smaller organic molecules (carboxylic acids). The stability of the catalyst observed to decrease as the number of cycles increased, this is due to the leaching of iron ions from the support material. Hence it was concluded that the activated carbon-iron oxide composite was successfully synthesized and had the high catalytic activity for the degradation of MB and 2-NP.

Μελετάται αριθμητικά η υδροδυναμική κυκλοφορία του Πατραϊκού κόλπου. Σκοπό αποτελεί η κατανόηση της τρισδιάστατης δομής της ροής που προκαλείται υπό διάφορες φυσικές φορτίσεις, δηλαδή τον άνεμο, την παλίρροια και τη στρωμάτωση. Η αριθμητική προσομοίωση γίνεται με τον τρισδιάστατο αριθμητικό κώδικας πεπερασμένων όγκων MIKE 3 FM (HD,TR). Η ανεμογενής κυκλοφορία, κατά τους χειμερινούς μήνες οπότε ο κόλπος σύμφωνα με μετρήσεις είναι ομογενής, παρουσιάζει στοιχεία ροής μεγάλης κλίμακας, με επιρροή από τη δύναμη Coriolis, ανάπτυξη στρώματος Ekman και ισχυρά ρεύματα επιστροφής, λίγα μέτρα κάτω από την ελεύθερη επιφάνεια. Ταυτόχρονα, σε περιοχές όπως η θέση του στενού Ρίου-Αντιρρίου η ροή συμπεριφέρεται περισσότερο ως υδραυλική ροή με ταπείνωση της ελεύθερης επιφάνειας και κατακόρυφο προφίλ ταχύτητας, όμοιο με της τυρβώδους ροής Couette. Στην οριζόντια διεύθυνση, η ροή παρουσιάζει έντονα παράκτια ρεύματα και κλίση της ελεύθερης επιφάνειας. Εκτιμάται ότι ο χρόνος ανανέωσης των υδάτων για τον κόλπο θα κυμαίνεται από ένα μήνα έως μία εβδομάδα, ανάλογα με την ένταση του ανέμου. Φαίνεται ότι στα βαθύτερα στρώματα του Πατραϊκού η ανεμογενής ροή δεν επιδρά σημαντικά στην ανανέωση των υδάτων, τα οποία παραμένουν εγκλωβισμένα και επανακυκλοφορούν μεταξύ των δύο εγκάρσιων ραχών που οριοθετούν την λεκάνη του. Τα παλιρροϊκά ρεύματα, τα οποία είναι σημαντικά στον Πατραϊκό, καθορίζουν την κυκλοφορία των υδάτων στο κύριο σώμα του κόλπου και στο στενό Ρίου-Αντιρρίου, ενώ η ανεμογενής ροή είναι καθοριστική κοντά στις ακτές. Η συνδυασμένη δράση ανέμου και παλίρροιας προκαλεί κυκλωνικούς και αντικυκλωνικούς στροβίλους, πλησίον των βορείων και νοτίων ακτών του Πατραϊκού, η φορά των οποίων υπαγορεύεται αποκλειστικά από τα ισχυρά ανεμογενή παράκτια ρεύματα. Η μελέτη της καλοκαιρινής κυκλοφορίας αναδεικνύει σημαντικές διαφορές, σε σύγκριση με την χειμερινή κυκλοφορία των υδάτων στον Πατραϊκό κόλπο. Τα ανεμογενή ρεύματα είναι αμελητέα από τη ζώνη του θερμοκλινούς και κάτω, για τη μέση ένταση ανέμου, ενώ η ένταση της τύρβης περιορίζεται στο επιλίμνιο. Την καλοκαιρινή περίοδο η δράση νοτιοδυτικού ανέμου συνδέεται με την παρουσία αναβλύσεων στις βορειοδυτικές ακτές του όρμου της Ναυπάκτου και στο μέτωπο της λιμνοθάλασσας του Μεσολογγίου. Αναβλύσεις εντοπίζονται και νοτιοδυτικά του Ρίου, μπροστά από την πόλη των Πατρών, για βορειοανατολικούς ανέμους. Στο στενό Ρίου-Αντιρρίου η δράση του ανέμου, αλλά και η παλίρροια οδηγούν στη γένεση εσωτερικών κυμάτων τα οποία προκαλούν κατακόρυφη κυκλοφορία. Κύριο χαρακτηριστικό της κυκλοφορίας των υδάτων στον Πατραϊκό, την καλοκαιρινή περίοδο, είναι ο ισχυρός κυκλωνικός στρόβιλος που παρατηρείται στο κέντρο του κόλπου αναγκάζοντας τα ύδατα σε περιστροφική κίνηση. Με βάση τις αριθμητικές προσομοιώσεις η ύπαρξη ισχυρού κυκλωνικού στροβίλου συνδέεται αποκλειστικά με την παρουσία βορειοανατολικού ανέμου και συγκεκριμένα στη φάση της πλημμυρίδας. Τέλος, με βάση τις αριθμητικές προσομοιώσεις υπολογίζεται το τρισδιάστατο πεδίο ρευμάτων στην ευρύτερη περιοχή του παλαιού και νέου λιμένα Πατρών, καθώς και η διάχυση και μεταφορά των επεξεργασμένων λυμάτων από τον αγωγό της ΔΕΥΑΠ. / The hydrodynamic circulation in the Gulf of Patras, in Western Greece, is studied using three-dimensional numerical simulations. The simulations are performed using the three-dimensional modeling system MIKE 3 FM (HD/TR). The wind-induced circulation is examined in the natural basin of the Gulf of Patras, in which batotropic flow develops, according to field measurements, during the winter months. The simulations show that the wind-induced flow creates strong currents near the coasts, which determine the sense of rotation of the gyres that develop in the Gulf. Strong currents are also created at the Rio-Antirio straits. The wind-induced, barotropic currents do not seem to contribute to the renewal of bottom waters, which recirculate between the two sills. Depending on the speed of the wind forcing the flow, the residence time of the waters in the Gulf of Patras is estimated to range from one month to one week. The tide-induced circulation is important in the Gulf of Patras. Strong, tidal currents have been simulated to develop at the straits of Rio-Antirrio and in the main body of the Gulf, with cyclonic and anticyclonic eddies developing at the northern and southern coasts, respectively. When there is also wind blowing, nearshore gyres develop, the sense of rotation of which is by the wind direction, while in the central part of the Gulf the flow pattern is dictated by the tidal action. Further, the baroclinic wind and tide-induced circulation are studied to investigate the effect of stratification on the structure of the flow. During the summer period the wind-induced flow and the wind generated turbulence driven by winds of medium strength are restricted to the upper layer of the epilimnion, while the strong winds cause tilt and erosion of the thermocline in the central part of the Gulf and generation of internal waves at the straits of Rio-Antirio. Salient features of the summer circulation are the strong upwellings and the central cyclonic gyre which are predicted using numerical simulations. Upwellings occur along the northwesterly portion of the Nafpaktos bay coasts at the westerly part of the Gulf of Corinth, under southwesterly wind, and to the south of Rio cape at the front of the city of Patras, under northeasterly wind. Concerning with the central cyclonic eddy, according to numerical simulations it develops exclusively during the flood tide under the simultaneous action of northeasterly winds. The numerical predictions were validated against satellite images of the surface temperature field determined by NOAA-6 and NOAA-7 which closely confirm the simulation results. Finally, the simulated barotropic and baroclinic flow fields are applied to the calculation of the currents near the port of Patras and to the advection and diffusion of the treated sewage effluents disposed of near the southeasterly coast of the Gulf, near the city of Patras.

Endocrine disrupting compounds and pesticides have been detected in rivers and irrigation canals of Southern Alberta, a semiarid region with irrigation-dependent crop production, intensive livestock operations, and a growing human population. However, little is known about the effects of agricultural runoff or wastewater treatment plant (WWTP) effluent in Southern Alberta on fish. Reproductive effects of WWTP effluents from the cities of Lethbridge and Medicine Hat, as well as agricultural runoff in the Lethbridge Northern Irrigation District canals, were investigated in a field study with wild fathead minnows (FHMN) in the Oldman and the South Saskatchewan rivers, in Alberta, Canada, and in a laboratory study with laboratory reared FHMN exposed in vivo to the city of Lethbridge WWTP effluent for 21 days. Biochemical and morphological endpoints were measured to characterize reproductive status. Liver vitellogenin, a biomarker of exposure to estrogen mimics, was analyzed using quantitative RT-PCR, and gonadal histology was used to determine sex, gonadal maturity, and intersex. Adverse reproductive effects were detected in FHMN exposed for 21 days to 10 and 25% of Lethbridge WWTP effluent. In the field, effluents from both Lethbridge and Medicine Hat had an effect on the reproductive systems of FHMN. In canals, reproductive effects were detected in wild fathead minnows in years when water quality in irrigation drain canals decreased. Exposure to pesticides was estimated using acetylcholinesterase (AChE) inhibition. Exposure to Lethbridge WWTP effluent did not inhibit AChE, whereas results from the field study were inconclusive. In conclusion, reproductive systems of fathead minnows in Southern Alberta were impacted by anthropogenic chemicals. / xi, 104 leaves : ill. (some col.), maps ; 29 cm

South Africa is water scarce country with maximum rainfall received in the summer season which lasts for only three months (November, December and January); hence the water resources have to be protected. The municipal wastewater effluents are considered one of the environmental threats that impact the water quality of the streams. This study was conducted to assess the environmental impact that the wastewater effluent has on the Klip River system, the performance of the plant and also to assess the spatial and temporal variations of water quality along the Klip River system.The study focused mainly on historical data over a five period (2009 – 2013) years secondary data which was analysed by Johannesburg Water Ltd (Pty) and primary data were also collected and analysed using the standard methods of laboratory analysis. The standard methods used include Ion selective electrode, gravimetric techniques, iodemetric titration, membrane filtration method; colorimetric method, automated flow injection method and inductively coupled plasma atomic emission spectrometry (ICP – AES). The aim of collecting the primary data during the dry and wet seasons was to verify the secondary data. The data set was further analysed using multivariate techniques such as principal component analysis (PCA), factor analysis (FA) and discriminant analysis (DA) to determine the spatial and temporal variation of water quality. The data set using ten water quality parameters (ammonia, sulphates, Chlorine, Chemical Oxygen Demand, conductivity, Escherichia coli, sodium, nitrates, pH and suspended solids) was grouped into four sampling points (influent, effluent, downstream and upstream points) and four seasons.Discriminant analysis of water quality showed that out of ten water quality parameters analysed, only sulphates was a less significant parameter to discriminate between the sampling points. For the temporal variations, eight water quality parameters (ammonium, Chlorine, Conductivity, sodium, nitrates, pH, sulphates and suspended solids are the most significant parameters to discriminate between the four seasons. PCA/FA results highlighted similarities in terms of water quality loading between summer and winter seasons and between the winter and autumn seasons. Summer and winter seasons had strong positive loading in COD, ammonium, suspended solids and E. coli whereas the autumn and spring seasons had strong positive loading in sodium, chlorine and pH. The study further highlighted that the Olifantsvlei Wastewater Treatment Works (WWTW) is effectively treating the wastewater up to the required standards before discharging them into the Klip River system. This study concludes that the Olifantsvlei WWTW does not contribute significant loads of pollutants into the Klip river system. / Environmental Sciences / M. Sc. (Environmental Science)

Water is an indispensible and yet a difficult resource to be renewed, thus water scarcity has become one of the major challenges faced worldwide, with the Southern regions of Africa being the most impacted and affected, especially the Eastern Cape Province of South Africa where rural communities depend on receiving waterbodies that are often negatively impacted by wastewater final effluents. This present study was conducted between August and December 2010 to assess the physicochemical and microbial qualities of the final effluents of peri-urban and rural communities based wastewater treatment plants in the Eastern Cape Province. The physicochemical parameters were determined on site and in the laboratory, while bacteriological qualities were determined using culture based techniques. The virological qualities were determined by molecular methods using reverse transcriptase polymerase chain reaction for the target RNA virus and the conventional polymerase chain reaction for the target DNA virus. For both wastewater treatment plants, the physicochemical parameters ranged as follows: chemical oxygen demand (5.95-45 mg/L); total dissolved solids (114.5-187.0 mg/L); salinity (0.12-0.20 psu); temperature (14.2-25.7oC); pH (6.0-7.6); nitrate and nitrites (1.55-6.7 mg/L and 0.023-1.15 mg/L respectively); biological oxygen demand (3.5-7.8 mg/L); turbidity (1.49-6.98 NTU); and chlorine residual (0-2.97 mg/L). Feacal indicator bacteria counts ranged as follows: feacal coliforms (0-1.25×104 cfu/100 ml); total coliforms (0-3.95×104 cfu/100 ml); and enterococci (0-5.0×103 cfu/100 ml). xviii Seventy five percent of the rural community based plant and 80 percent of the peri-urban community based plant were positive for coxsackie A virus, while hepatitis A virus was detected in all the rural community based plant 80 percent of the peri-urban community based plant. This study suggests the need for intervention by appropriate regulatory agencies to ensure regular monitoring of the qualities of final effluents of wastewater treatment facilities in the Eastern Cape Province and ensure compliance to established guidelines.

Modern pig farming production may over burden the environment with organic substances, exposure of bacterial pathogens and introduction of resistance gene. This may be caused by the pig’s droppings, lack of seepage management or accidental spillage of seepage which may impact on the environment and its physicochemical parameters. The objective of this study is to determine and assess the level of bacteriological pollution emanating from the pig farm and their impact on the physicochemical parameters of soil and water as well as to identify the presence of antibiotic resistance gene of these prevailing bacteria. Soil and water samples were collected monthly for a period of six months (March- August 2013). Samples were collected at pig enclosures, soil 20 m and 100 m away from pig enclosures, constructed wetland used for treating pig farm wastewater, soil 20m and 100 m away from constructed wetland. Procedure followed for analysing soil and water samples includes physicochemical analyses, viable cell counts of 10-1 to 10-8 dilutions, identification of bacteria using API 20E test kit, antibiotic susceptibility analyses, and identification of resistance gene using molecular procedures. The media that were used for viable cell counts were, Nutrient agar, MacConkey Agar, Xylose Lysine Deoxycholate agar (XLD agar), and Eosin Methylene Blue (EMB). Physicochemical parameters of water showed unacceptable high levels of analysed parameters for BOD (163 mg/L to 3350 mg/L), TDS (0.77 g/L to 6.48 mg/L), COD (210 mg/L to 9400 mg/L), NO3 (55 mg/L to 1680 mg/L), NO2 (37.5 mg/L to 2730 mg/L), and PO43− (50 mg/L to 1427 mg/L) were higher than the maximum permissible limits set by Department of Water Affairs and Forestry (DWAF). For soil samples TDS (0.01g/L to 0.88 g/L), COD (40 mg/L to 304 mg/L), NO3 (32.5 mg/L to 475 mg/L), and NO2 (7.35 mg/L to 255 mg/L) and PO43- (32.5 mg/L to 475 mg/L ) were observed to be higher than recommended limits set by Federal Ministry for the Environmental (FME). The viable cells in soil samples 30cm depth ranged from 0 cfu/mL to 2.44 x 1010cfu/mL, in soil 5cm depth ranged from 1.00 x 101 cfu/mL to 1.91 x 1010 cfu/mL, and in water samples viable cells ranged from 5.00 x 101 to 5.05 x 109. Pseudomonas luteola (Ps. luteola), Escherichia vulneris (E. vulneris), Salmonella choleraesuis spp arizonae, Escherichia coli 1(E. coli 1), Enterobacter cloacae, Pseudomonas flourescens/putida (Ps. flourescens/putida), Enterobacter aerogenes, Serratia ordoriferal, Pasteurella pneumotropica, Ochrobactrum antropi, Proteus vulgaris group, Proteus vulgaris, Salmonella spp, Aeromonas Hydrophila/caviae/sobria1, Proteus Mirabillis, Vibrio fluvials, Rahnella aquatillis, Pseudomonas aeruginosa (Ps. aeruginosa), Burkholderia Cepacia, Stenotrophomonas maltophilia (St. maltophilia), Shwenella putrefaciens, Klebsiela pneumonia, Cedecea davisa, Serratia liquefaciens, Serratia plymuthica, Enterobacter sakaziki, Citrobacter braakii, Enterobacter amnigenus 2, Yersinia pestis, Serratia ficaria, Enterobacter gergoriae, Enterobacter amnigenus 1, Serratia marcescens, Raoutella terrigena, Hafnia alvei 1, Providencia rettgeri, and Pantoa were isolated from soil and water samples from the pig farm. Isolates were highly resistant to Penicillin G, Sulphamethaxazole, Vancomycin, Tilmocozin, Oxytetracycline, Spectinomycin, Lincomycin, and Trimethoprim. The most resistance genes detected in most isolates were aa (6’)-le-aph (2”)-la, aph (2”)-lb, aph (3”)-llla, Van A, Van B, Otr A and Otr B. Pig farm seepage is causing bacterial pollution which is impacting negatively on the natural environment in the vicinity of pig farm by introducing bacterial pathogens that have an antibiotic resistance gene and is increasing the physicochemical parameters for soil and water in the natural environment at the pig farm. It is therefore recommended that pig farms should consider the need to implement appropriate regulatory agencies that may include the regular monitoring of the qualities of final effluents from waste water treatment facilities. In addition there is a need to limit soil pollution in order to safe guard the natural environment in the vicinity of pig farm from bacteriological pollution and introduction of antibiotic resistance gene. It is also recommended that more advanced technologies should be introduced that will assist pig farms to manages the seepage properly. / Environmental Sciences / M. Sc. (Environmental Sciences)

Progress has been made in eradicating the backlog in sanitation. This is reflected by the 2011 Census results that reflect a 21 percent increase in the provision of sanitation between the 2001 Census and the 2011 Census. In the rural areas of O.R. Tambo District Municipality, the eradication of sanitation backlogs is done through the provision of VIPs for sanitation. Even with this progress, there are still challenges resulting from the previously constructed sanitation facilities. Some of the challenges relate to the poor quality of these facilities. Community members – via the IDP road shows have voiced their unhappiness with regard to some of the sustainability factors of these structures. Community members feel they were not involved in decisions related to the sanitation projects. It has also been observed that the municipality and its agents do not conduct environmental management studies; and some pits of these structures were not lined, and the health and hygiene training, together with post-construction assessment was not done. In this study, an assessment of the provision of sanitation facilities, in order to identify some of the key factors that are critical to the sustainability of rural sanitation facilities, was done. The research findings should be helpful in assisting local government to improve the provision of sanitation facilities, with the aim of ensuring sustainability, improving community satisfaction, and improving the quality of life for the people living in these villages. For the purpose of the study, a quantitative research approach was used. A research questionnaire was used to collect the data; and it was distributed through email to a sample of respondents that understood sanitation issues. The data were collected from municipal officials, consultants working on sanitation projects, as well as other stakeholders with influence and an interest in sanitation. The results were analysed to establish the findings and to propose recommendations, in order to improve the sustainability of rural sanitation facilities. From the research findings, the recommendations highlighted the need for an improved participatory approach in implementing sanitation projects. To ensure sustainability, the following issues are key: monitoring and supervision; community participation and adherence to design guidelines; a review of the sanitation provision policy; as well as the promotion of health and hygiene.

Worldwide, water reuse is promoted as an alternative for water scarcity, however, wastewater effluents have been reported as possible contaminants to surface water. The failure of some wastewater treatment processes to completely remove organic matter and some pathogenic microorganisms allows them to initiate infections. This manifests more in communities where surface water is used directly for drinking. To assess water quality, bacteria alone cannot be used as it may be absent in virus-contaminated water. This study was carried out to assess the quality of two wastewater treatment plant effluents from the Eastern Cape Province of South Africa. Physicochemical parameters and microbiological parameters like faecal coliforms, adenovirus, rotavirus, hepatitis A virus, norovirus and enterovirus were evaluated over a projected period of one year. Physicochemical parameters were measured on site using multiparameters, faecal coliforms enumerated using culture-based methods and viruses are detected using both conventional and real-time PCR. Physicochemical parameters like electrical conductivity, turbidity, free chlorine and phosphates were incompliant with the standards set by the Department of Water affairs for effluents to be discharged. Faecal coliform counts were nil for one plant (WWTP-R) where they correlated inversely (P < 0.01) with the high free chlorine. For WWTP-K, faecal coliforms were detected in 27% of samples in the range of 9.9 × 101 to 6.4× 104 CFU/100ml. From the five viruses assessed, three viruses were detected with Rotavirus being the most abundant (0-2034176 genome copies/L) followed by Adenovirus (0–275 genome copies/L) then Hepatitis A virus (0–71 genome copies/L) in the WWTP-K while none of the viruses was detected in WWTP-R. Species B, species C and Adv41 serotypes were detected from the May 2013 and June 2013 samples where almost all parameters were incompliant in the plant. The detection of these viruses in supposedly treated effluents is suggestive of these being the sources of contamination to surface water and therefore renders surface waters unsafe for direct use and to aquatic life. Although real-time PCR is more sensitive and reliable in detection of viruses, use of cell-culture techniques in this study would have been more efficient in confirming the infectivity of the viruses detected, hence the recommendation of these techniques in future projects of this nature.