FundaÃÃo de Amparo a CiÃncia e Tecnologia de Pernambuco / O ensino e as pesquisas na Ãrea de enfermagem tÃm procurado adequar-se aos avanÃos das tecnologias de informaÃÃo e comunicaÃÃo. Percebeu-se entÃo a importÃncia de investigar a utilizaÃÃo de uma tecnologia digital na aprendizagem dos futuros profissionais de saÃde, em face da repercussÃo das infecÃÃes sexuais no sistema de saÃde e a relevÃncia da temÃtica para a formaÃÃo do enfermeiro. O foco desta investigaÃÃo foi avaliar o resultado da utilizaÃÃo de hipermÃdia educativa para aprendizagem de acadÃmicos de enfermagem sobre doenÃas sexualmente transmissÃveis. Trata-se de um estudo quase-experimental, aprovado pelo Comità de Ãtica em Pesquisa da Universidade Federal do CearÃ. Estrutura-se em quatro etapas: 1) construÃÃo da hipermÃdia; 2) validaÃÃo por especialistas; 3) avaliaÃÃo da aprendizagem; e 4) avaliaÃÃo pelos alunos. Na primeira etapa, fez-se um amplo resgate da literatura de modo a disponibilizar as principais referÃncias e consensos existentes sobre a temÃtica. Selecionaram-se as mÃdias e elaboraram-se o guia do aluno, os objetivos acadÃmicos e o conteÃdo dos mÃdulos. A hipermÃdia foi hospedada no ambiente virtual de aprendizagem SOLAR. Na segunda etapa, a hipermÃdia foi validada por nove especialistas de conteÃdo e trÃs especialistas tÃcnicos, definidos a partir de parÃmetros adaptados da literatura. Obteve-se Ãndice de validade do conteÃdo geral de 0,97 nos itens avaliados (objetivo, conteÃdo, relevÃncia, ambiente, funcionalidade, usabilidade e eficiÃncia). Na terceira etapa, participaram 58 acadÃmicos de enfermagem divididos em dois grupos semelhantes (controle e intervenÃÃo). O grupo controle participou da aula tradicional e o grupo intervenÃÃo utilizou a hipermÃdia no ambiente virtual de aprendizagem. Ambos responderam a um questionÃrio validado sobre o tema (prà e pÃs-teste). No pÃs-teste, a mÃdia de acertos obteve ganho de 4,20 (30,95%) questÃes no grupo controle e 6,65 (54,46%) questÃes no grupo intervenÃÃo. Hà evidÃncia de aumento da mÃdia no pÃs-teste (p=0,00) do grupo intervenÃÃo apontando a aprendizagem online como estratÃgia tÃo eficaz quanto a presencial e que pode ser utilizada como recurso de ensino complementar na formaÃÃo de enfermeiros. Na quarta etapa, a maioria dos acadÃmicos de enfermagem avaliou a hipermÃdia como adequada, tendo como base os antecedentes do conceito aprendizagem online (interaÃÃo e estÃmulo, ferramentas de comunicaÃÃo, material didÃtico, interesse e motivaÃÃo para aprender, dedicaÃÃo, disciplina e gerenciamento de tempo, papel do aluno). A atitude do aluno referente à motivaÃÃo (p=0,001) e ao fato dele acreditar ser responsÃvel por sua aprendizagem (p=0,004) tiveram relaÃÃo estatisticamente significativa no aumento do nÃmero de acertos do pÃs-teste. Conclui-se que a hipermÃdia sobre doenÃas sexualmente transmissÃveis està apta a facilitar o processo de ensino/aprendizagem dos acadÃmicos de enfermagem. Constitui-se em uma ferramenta de apoio ao ensino presencial que colabora com o processo de formaÃÃo profissional, tornando a aprendizagem ativa e significativa.

CoordenaÃÃo de AperfeiÃoamento de Pessoal de NÃvel Superior / Objetivou-se comparar os achados de infecÃÃes vaginais em gestantes obtidos por meio do fluxograma de corrimento vaginal com exames presentes na prÃtica clÃnica. O estudo foi do tipo avaliativo, com delineamento transversal e abordagem quantitativa, desenvolvido no Centro de Parto Natural LÃgia Barros Costa em Fortaleza - CE, com amostra composta por 104 gestantes. Os dados foram coletados por meio de entrevista e exame ginecolÃgico de Janeiro a Julho de 2011. Foi realizada anÃlise estatÃstica, utilizando frequÃncia absoluta, mÃdia e Desvio PadrÃo. Realizou-se, tambÃm, testes de sensibilidade, especificidade, valores preditivos positivo e negativo, acurÃcia e as razÃes de verossimilhanÃa positiva e negativa, em que se estabeleceu significÃncia para valor de p<0,1. O projeto de pesquisa foi aprovado pelo Comità de Ãtica em Pesquisa da Universidade Federal do CearÃ, conforme protocolo n 298/10. As gestantes apresentaram mÃdia de idade de 23,7 anos, parceria fixa (78; 75%), mÃdia de 12,3 anos de estudo e renda familiar entre um e dois salÃrios mÃnimos (59; 56,7%). A menarca ocorreu em mÃdia com 12,8 anos e a sexarca com 16 anos. NÃo apresentavam histÃria pregressa de IST (90; 86,5%) e nÃo realizavam exame citolÃgico periodicamente (58; 55,8%). Houve predomÃnio de mulheres multÃparas (58; 55,8%), com uma mÃdia de 20,24 semanas gestacionais. As queixas ginecolÃgicas mais predominantes foram: corrimento vaginal (87; 83,7%), odor de secreÃÃo vaginal (45; 43,3%), dispareunia (36; 34,6%), prurido (27; 26%), disÃria (12; 11,5%), sinusorragia (6; 5,8%) e febre (4; 3,9%). Encontrou-se uma prevalÃncia de 13,5% de positividade para pelo menos uma das trÃs infecÃÃes vaginais investigadas de acordo com o exame a fresco. Observou-se predominantemente o pH de valor 5 (50; 48,1%) e o teste de aminas negativo (66; 63,5%). O fluxograma nÃo se mostrou eficaz na identificaÃÃo de candidÃase, apresentando uma baixa sensibilidade (0,0%) e Valor Preditivo Positivo (0,0%), uma alta especificidade (97,9%), Valor Preditivo Negativo (90,2%) e acurÃcia (88,5%), alÃm de RazÃo de VerossimilhanÃa Positiva nula e RazÃo de VerossimilhanÃa Negativa igual a 1. Para tricomonÃase, apresentou baixa sensibilidade (50%), especificidade (46%), Valor Preditivo Positivo (3,6%) e acurÃcia (46,2%), um alto Valor Preditivo Negativo (95,8%), RazÃo de VerossimilhanÃa Positiva igual a 0,9 e RazÃo de VerossimilhanÃa Negativa de 1,08. Para vaginose bacteriana, mostrou-se satisfatÃrio, com uma alta sensibilidade (100%), Valor Preditivo Negativo (100%) e acurÃcia (74%), uma baixa sensibilidade (64%) e Valor Preditivo Positivo (51,8%), alÃm de RazÃo de VerossimilhanÃa Positiva igual a 2,7 e RazÃo de VerossimilhanÃa Negativa nula. Conclui-se que o emprego da abordagem sindrÃmica das infecÃÃes vaginais em gestantes precisa ser reavaliada, visto que o fluxograma nÃo foi eficaz em identificar infecÃÃes como candidÃase e tricomonÃase. Os esforÃos para o desenvolvimento de testes simples e mais acessÃveis devem ser contÃnuos. Entretanto, devem-se empregar tÃcnicas mais avanÃadas, como o exame a fresco, na prÃtica clÃnica, com intuito de aprimorar as prÃticas em saÃde sexual e reprodutiva, evitando a disseminaÃÃo de infecÃÃes, reduzindo tratamentos desnecessÃrios e melhorando a qualidade de vida das gestantes. / The aim was to compare the findings of vaginal infections in pregnant women obtained from the vaginal discharge flowchart with tests present in the clinical practice. This was an evaluation study, with a transversal delineation and quantitative boarding, developed in the Center of Natural Birth LÃgia Barros Costa in Fortaleza â CE, with a sample of 104 pregnant women. The data was collected through interviews and gynecological examination from January to July of 2011. Statistical analysis was carried through, using absolute and average frequency and Standard Deviation. It was also conducted tests of sensitivity, specificity, predictive positive and negative values, accuracy and reasons of positive and negative probability, where it was established a significance value of p< 0,1. The research project was approved by the Research Ethics Committee of the Federal University of the CearÃ, under the protocol n 298/10. The pregnant women presented an average age of 23,7 years, fixed partnership (78; 75%), average of 12,3 years of study and family income between one and two minimum wages (59; 56,7%). The menarche occurred with an average age of 12,8 years and the first sexual intercourse with 16 years. They did not present history of STDs (90; 86.5%) and did not take cytological examinations regularly (58; 55,8%). The majority of the women are multiparous (58; 55.8%), with an average of 20,24 weeks of gestation. The predominant gynecological complaints were: vaginal discharge (87; 83.7%), vaginal secretion odor (45; 43.3%), dyspareunia (36; 34.6%), itch (27; 26%), dysuria (12; 11.5%), sinusorragia (6; 5.8%) and fever (4; 3,9%). It was observed a prevalence of 13,5% of positivity for at least one of three vaginal infections investigated according to the fresh examination. It was observed predominantly a pH value of 5 (50; 48.1%) and whiff test negative (66; 63,5%). The flowchart did not reveal efficient in the identification of candidiasis, presenting a low sensitivity (0.0%) and positive predictive value (0.0%), a high specificity (97.9%), negative predictive value (90.2%) and accuracy (88.5%), as well as null positive likelihood ratio and negative likelihood ratio equal to 1. For trichomoniasis, it presented low sensitivity (50%), specificity (46%), positive predictive value (3.6%) and accuracy (46.2%), a high negative predictive value (95.8%), positive likelihood ratio equal to 0,9 and negativo likelihood ratio 1,08. For bacterial vaginosis, it revealed satisfactory, with a high sensitivity (100%), negative predictive value (100%) and accuracy (74%), a low sensitivity (64%) and positive predictive value (51.8%), as well as positive likelihood ratio equal to 2,7 and null negative likelihood ratio. In conclusion, the use of a syndromic boarding for vaginal infections in pregnant women needs to be reevaluated, since the flowchart was not efficient in identifying infections such as candidiasis and trichomoniasis. The efforts for the development of simple and accessible tests must be continuous. However, more advance and techniques, like the fresh examination, in the practical clinic, aiming to contribute to the improvement of the sexual health and reproductive practices, preventing the dissemination of infections, reducing unnecessary treatments and improving the quality of life of these women.

As doenÃas sexualmente transmissÃveis (DST) sÃo importante causa de morbidade em todo o mundo, com considerÃveis repercussÃes mÃdicas, sociais e econÃmicas. Para traÃar o perfil epidemiolÃgico das DST em mulheres em idade fÃrtil de um pequeno municÃpio do interior do CearÃ, foi realizado um estudo de base populacional em Pacoti em janeiro e fevereiro de 2001. O estudo consistiu em questionÃrios estruturados, exame ginecolÃgico e diagnÃstico laboratorial para detecÃÃo de HPV, clamÃdia, tricomonas, gonorrÃia, sÃfilis e HIV. Participaram do estudo 734 mulheres entre 12 e 49 anos da zona urbana e rural do municÃpio (75% da populaÃÃo-alvo). Dentre essas, 592 haviam iniciado vida sexual e foram incluÃdas na anÃlise. As taxas de prevalÃncia foram: HPV 11,7% (IC 95%: 9,3-14,7), clamÃdia 4,5% (IC 95%: 3,0-6,6), tricomonas 4,1% (IC 95%: 2,7-6,1), gonorrÃia 1,2% (IC 95%: 0,5-2,6) e sÃfilis 0,2% (IC 95%: 0,0-1,1). Nenhum exame para HIV foi positivo. Apresentaram pelo menos uma DST 19,6% (IC 95%: 16,5-23,2) das mulheres. As DST foram mais prevalentes entre as adolescentes. A prevenÃÃo do cÃncer ginecolÃgico anterior mostrou forte efeito protetor contra ter DST (OR=0,4; IC 95%: 0,2-0,6). Foram identificados os seguintes fatores de risco: >2 parceiros na vida (OR=2,8; IC 95%: 1,8-4,4), idade da primeira gestaÃÃo <16 anos (OR=2,1; IC 95%: 1,1-4,1), nÃo saber se o parceiro tem outra parceira (OR=1,7; IC 95%: 1,0- 2,8), idade <20 anos (OR=1,9; IC 95%: 1,1-3,4), >1 parceiro nos Ãltimos 12 meses (OR=3,0, IC 95%: 1,4-6,7), inÃcio precoce da vida sexual (OR=2,0; IC 95%: 1,3-3,1) e nÃo habitar com o parceiro (OR=1,7; IC 95%: 1,1-2,7). Nenhum fator sÃcio-econÃmico mostrou associaÃÃo significativa a ter uma DST. O fator protetor e os primeiros trÃs fatores de risco acima mencionados foram independentemente associados a ter DST no modelo multivariado. As DST representam um importante problema na populaÃÃo estudada, e a identificaÃÃo dos fatores protetores e de risco pode orientar a implementaÃÃo de medidas de controle especÃficas que respeitem particularidades de gÃnero e faixa etÃria. O presente estudo permite inferir sobre a epidemiologia das DST em outros municÃpios de perfil semelhante. / Sexually transmitted diseases (STD) are an important cause of morbidity throughout the world with considerable medical and socio-economic repercussions. To assess the epidemiological situation of STD among women of reproductive age in a small municipality in the hinterland of CearÃ, a population-based study was conducted in January and February 2001. The study consisted of application of structured questionnaires, gynecological examination and laboratory diagnosis to detect HPV, chlamydia, trichomonas, gonorrhea, syphilis and HIV. A total of 734 women aged 12 to 49 years participated in the study (75% of the target population). Among these, 592 had initiated sexual life and were included in the present analysis. The following prevalences were found: HPV 11,7% (IC 95%: 9,3-14,7), chlamydia 4,5% (IC 95%: 3,0-6,6), trichomonas 4,1% (IC 95%: 2,7-6,1), gonorrhea 1,2% (IC 95%: 0,5-2,6) and syphilis 0,2% (IC 95%: 0,0-1,1). There was no case of HIV infection. At least one STD was detected in 19,6% (IC 95%: 16,5-23,2). STD were more prevalent among adolescents. A previous pap smear collection showed an important protective effect against STD (OR=0,4; IC 95%: 0,2-0,6). The following risk factors were identified: >2 partners in life (OR=2,8; IC 95%: 1,8-4,4), first pregnancy <16 years of age (OR=2,1; IC 95%: 1,1-4,1), not knowing if the partner has another partner (OR=1,7; IC 95%: 1,0-2,8), being <20 years of age (OR=1,9; IC 95%: 1,1-3,4), >1 partner in the last 12 months (OR=3,0; IC 95%: 1,4-6,7), early sexual debut (OR=2,0; IC 95%: 1,3-3,1) and not living with a partner (OR=1,7; IC 95%: 1,1-2,7). There was no significant association between STD and socio-economic variables. The above mentioned protective factor and the first three risk factors were independently associated with STD in the multivariate analysis. STD represent an important problem in the population studied, and the identification of protective and risk factors can guide the implementation of gender and age specific control measures. The present study allows to conclude about the STD epidemiology in other municipalities with similar characteristics.

Magister Public Health - MPH / Background: The HIV / AIDS pandemic have been characterized as the greatest natural challenge ever to confront humanity and one of the great moral causes of our time (World Bank, 2007). Although, the HIV and AIDS pandemic is a global problem, some regions of the world, notably sub-Saharan Africa are hardly hit by the pandemic. Namibia is one of the sub-Saharan African countries which are badly affected by the HIV and AIDS pandemic. It is estimated that about 360 000 Namibians were infected with HIV by 2010. This translates to a national HIV prevalence of (18.8%). The National HIV and AIDS Response Department has designed various interventions and strategies to curb the spread of the HIV. Promotion of male condoms promotion is one of the key strategies being advocated. If used consistently and correctly, male condoms are regarded as an effective preventive tool of HIV transmission. However, changing individual’s sexual behaviors and lifestyle to make condom use part of their sexual life seems to be the challenge. In Namibia most of the research in the area of HIV and AIDS were done among the youth and population aged between 15 – 49 years. Hence, this study looks at older men as a neglected population. Aim: The aim of the current study was to assess knowledge, attitudes and practices of condom use among male employees age 50 (fifty) years and older at a mining company in Southern Namibia. Methodology: A descriptive, cross-sectional survey utilizing quantitative research approach was applied. Data was collected through face-to-face interviewing male employees, age fifty years and older, while at work at the Mine Area 1 (MA1). Data was captured in excel and imported into SPSS version 16.0. Chi-square test was used to determine the association between KAP variables and the socio-demographic characteristics of the respondents. The level of statistical significance was set at 0.05. Results: Among 105 respondents (males, mean age 54.5 years), more than half lived in the singe sex male hostels. Knowledge about condom use was good but there remained a significant number of the respondents whose knowledge was insufficient. A high percentage of the respondents suggested that HIV positive individuals should always use condom every time they have sexual intercourse. Conclusion: This study showed that respondents are not at a high risk of contracting HIV infection due to lack of knowledge on condom use though some of them possessed insufficient knowledge. Thus, to minimize the likelihood of HIV infection targeted interventions including peer education programmes were suggested, and working with the community in the area. Moreover, a more comprehensive knowledge attitude and practice study among mine employees from various sections of the mine would be beneficial to identify the level of risk within the total employee population.

The topic of the study is The Impact of HIV/AIDS and STIs in Secondary Schools in the Limpopo Province. The impact of HIV/AIDS and STDs on educators and learners in secondary schools is problematic. These epidemics are associated with an increase in absenteeism, requests for time off, emotional problems, changes in attitudes, overload on the side of teachers, unexpected deaths, increasing demands being made on medical aid schemes, as well as disability and malfunctioning of people and structures. The standard of education is deteriorating because of these diseases. Similarly, there is the need for managing the effects of these epidemics. This study employs participatory action research. The quantitative method is utilized in the study. The questionnaire is used as an instrument of measurement. The most important findings are as follows: • There is an increase in absenteeism for both learners and educators. • Learners’ performance is deteriorating. • Participation of learners in sport and extra-mural activities is decreasing. • Teachers’ productivity is deteriorating. • Depression is taking its toll both among learners and educators. • There is a high rate of dropouts. / Dr. M.C. van Loggerenberg

Background: Sexually transmitted diseases (STD's) have a major impact on sexual and reproductive health worldwide. Each year, the World Health Organization (WHO) estimates 448 million new cases of curable STD's are diagnosed. The emergence of drug resistance in STD related microorganisms and potential side effects demand the discovery of newer drugs. The exploration of newer anti-microbial substances from natural sources may serve as promising alternatives. In this study, twelve medicinal plant species used traditionally in the treatment of STD's are investigated in this regard. Methods: Ethanol plant extracts and three flavonoids were evaluated for their antimicrobial properties against one fungi and three bacteria, through the micro-dilution assay. To determine the anti-inflammatory activities of the extracts and compounds, the inhibitory effect was measured on the pro-inflammatory enzyme lipoxygenase, 15-LOX. Extracts were further evaluated for their inhibitory effect on the supercoiling activity of bacterial DNA gyrase by using the DNA gyrase kit. The extracts and compounds were lastly investigated for their anti-HIV activities against recombinant HIV-1 enzyme using non-radioactive HIV-RT colorimetric assay. Results: Acacia karroo and Rhoicissus tridentata extracts showed good antimicrobial activity with MIC values ranging between 0.4 and 3.1 mg/ml. Extracts of Jasminum fluminense, Solanum tomentosum and flavonoid 2 and 3 had good anti-inflammatory activity with IC50 less than the positive control quercetin (IC50 = 48.86 ug/ml). Extracts of Diospyros mespiliformis, Peltophorum africanum, Rhoicissus tridentata and flavonoids 1 and 2 showed the best inhibitory activity against the bacterial DNA gyrase. A. karroo and flavonoid 3 exhibited moderate HIV RT inhibition activity of 66.8 and 63.7 % respectively. R. tridentata and Terminalia sericea had the best RT inhibition activity (75.7 and 100 %) compared to the positive control doxorubicin (96.5%) at 100 ug/ml concentration. Conclusion: The observed activities may lead to new multi-target drugs against sexually transmitted diseases. / Dissertation (MSc)--University of Pretoria, 2017. / Plant Science / MSc / Unrestricted

Thesis advisor: Summer S. Hawkins / Thesis advisor: David Takeuchi / Adolescence is a critical developmental stage where the health behaviors and choices that adolescents make have the potential to affect their long-term health and well-being (Centers for Disease Control and Prevention, 2014). This dissertation contributes three distinct studies on the contextual influences that shape adolescents' health behaviors. The first study, "The Role of Psychosocial Conditions on Sexually Transmitted Infection (STI) Risk among U.S. Young Adults," grounded in life course and syndemics theory, utilized logistic regressions to examine the role of co-occurring psychosocial conditions (childhood sexual abuse and physical abuse; depression and illicit drug use in adolescence) on STI infection (chlamydia and trichomoniasis) and sexual risk behaviors among U.S. young adults. Multiple co-occurring psychosocial conditions had an additive effect on sexual risk behaviors but no effect was observed on STIs. The second study, "Sexually Transmitted Infections and Neighborhood Poverty: The Role of Individual Resilience and Social Connectedness," utilized resilience and ecological systems theory, and logistic regressions to test if individual resilience and social connectedness (maternal, peer, and school) moderate the association between concentrated neighborhood poverty and STIs (chlamydia and trichomoniasis) among U.S. young adults. The study's main finding is that youth who reported more school connectedness and lived in high concentrated poverty in adolescence were less likely to test positive for chlamydia but were more likely to test positive for trichomoniasis. Utilizing a similar framework, "Sleeping in a Digital World: The Role of Excessive Media Use on Sleep Inadequacy Among U.S. Adolescents," examined family and neighborhood determinants that shape adolescent sleep behaviors. Grounded in the ecological systems theory and social learning theory, logistic regressions, stratified by age (aged 10-12 vs. 13-17), were used to examine the associations between excessive media use and sleep inadequacy. The study found that among older adolescents, sleep inadequacy was associated with excessive computer use. Older adolescents who watched television excessively and had media present in the bedroom were more likely to be sleep inadequate. Together, these three studies shed light on the different contextual environments in which adolescents experience health risks and resilience and will help to inform interventions that promote adolescent health and well-being. / Thesis (PhD) — Boston College, 2015. / Submitted to: Boston College. Graduate School of Social Work. / Discipline: Social Work.

Submitted in partial fullfillment of the requirements of a Masters degree in Clinical Psychology University of Zululand, 2003. / Research shows that prevention campaigns have been successful in raising awareness about AIDS and even in teaching people how to protect themselves, i.e. "Abstain, Be Faithful, Condomise" where they have failed dismally is in convincing people especially the youth of today as to how to apply it. The aim of the study was to investigate qualitatively the sexual practices of adolescents in relation to sexually transmitted diseases and acquired immuno-deficiency syndrome. The study investigated the determinants of condom using behaviour amongst school going adolescents, in the Durban and Verulam metropolitan region of Kwa-Zulu Natal. The motivation for the study was contexualised within the preventative health model which views the consistent and correct use of condoms as a primary strategy in preventing the spread of STD's and HIV/AIDS. In South Africa the health promotion campaigns focusing on safe sex practices and condom use have met with limited success as evidenced in this study. The sexual behaviour of adolescents is a key factor in influencing HIV transmission and sexually transmitted diseases. The reason for this behaviour is that most youths are inconsistent condom users and those who become sexually active at an early stage, run a greater risk to being exposed to HIV and STD's, both because of multiple partners and increased episodes of unprotected sex. The results indicate that on a very superficial level, South African adolescents possess good knowledge about transmission and prevention. The reason for this is that in order to produce behavioural changes, education programmes on sex and AIDS should include cognitive and behavioural skills training, addressing interpersonal problem solving, planning and assertive communication. Furthermore the under-resourced health sector, inaccessible health facilities, poor education and preventative measures do not empower the youth to make health choices responsibly It is envisaged that the recommendations from the research would ensure that existing health facilities are accessible to the youth today. Family planning clinics which are decentralised and offer a youth day programme in respect of sexuality education and proper use of contraceptives and condoms, ensuring full confidentiality. It is further hoped that this research would show that prevention and education messages are more effectively conveyed to the youth in South Africa, emphasising cognitive and behavioural skills training, with the co-operation and co-ordinated efforts of all departments and non governmental organisation, as well as by those infected themselves.

Trichomonas vagina/is is a common sexually transmitted disease, affecting women more often than men. It has only been seen to undergo mitosis, even though published studies have confinned the organism has meiotic proteins. These meiotic proteins are known to function in other organisms with a key protein in homologous recombination, DMCl. RT-PCR analysis shows low expression ofDMCl in mitotically-growing cultures, and we found that some stresses on the organism increase DMCl expression. Polyclonal antibodies raised against DMCl protein have been used to test whole celllysates of the Tl and G3 strains of Trichomonas vagina/is but no obvious expression has been detected. We also used western blot analysis to show that superoxide dismutase is expressed in the standard lab strains Tl and G3 and immunocytochemistry studies showed that HA-tagged SOD6 protein localizes in the cytoplasm. Lastly, we found that SOD protein abundance increased in the CDC085 strain compared to Tl and G3, especially under aerobic conditions.

No description available.