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[Truncated abstract] Centrifugal pumps are designed to operate at a capacity that maximizes the efficiency of the pump. Operation below this design capacity results in reduction of pump efficiency as the geometric configuration of the impeller and casing no longer provide an ideal flow pattern. Consequently there are changes in the flow field within the pump, including flow separation and regions of localized, non-uniform, unsteady flow. This flow interacts with rotating and stationary components inside the pump creating additional disturbance and hydraulic excitation. It is anticipated that the local hydrodynamic and global hydroacoustic excitation due to partial-flow operation will affect the structural vibration measured on the pump. In this study, the effects of partial-flow operation on the vibration signal at the pump bearing housing are measured on a number of industrial double-suction pump units. These are a particular class of centrifugal pump commonly used in high volume applications such as water distribution. The aims are to understand how the vibration signals change at the different operating conditions and to determine which parameters are best suited to monitoring the observed changes. Comparison is made between the response, under similar operating conditions, of pumps both within sets of identical size and design, across sets of similar design and different sizes, and between different designs. The pumps are all in-service industrial units. In double-suction pumps the impeller motion in the axial direction (along the line of the shaft) is not constrained. Due to symmetry in impeller design, axial stability is maintained by equal and opposite hydraulic forces on the two opposing sides of the impeller. The potential for loss of axial hydraulic balance during partial-flow operation is examined from a theoretical perspective, based on a literature review, and by measurement of the axial displacement of the shaft. Structural vibration is measured using accelerometers mounted at the non-drive end bearing housing in the horizontal and axial orientations. Changes in signal contribution and characteristics are examined using a variety of qualitative and quantitative techniques. Test signals are used to assess the limitations of the techniques and the effect of parameter selection on the interpretation of the signals.

In this report, studies on interharmonics from three different measurement sites are performed. The first site is a wind park with three turbines, where the measurements are performed at the point of common coupling of these three. The second site is a network which consists of a PV inverter and two types of EV chargers – a DC charger or an AC charger. Measurements are performed with three different set-ups in this site – only AC charger connected, only DC charger connected, and AC charger and PV inverter connected simultaneously. The third site where measurements were made is a microgrid using frequency control in order to signal how the microgrid should operate at the moment. The interharmonic analysis was conducted using desynchronized processing technique (DP) and Sliding-Window Estimation of Signal Parameters via Rotational Invariance Techniques (SlidingWindow ESPRIT or SWESPRIT). The result from the wind park is that closely and evenly spaced interharmonics can be seen when the current suddenly increases (could be fast variations in wind speed). It is however uncertain if these interharmonics are caused by spectral leakage or not since SWESPRIT estimates the fundamental frequency to vary drastically when wind speed varies. It is observed that the SWESPRIT estimation of fundamental frequency could be caused by sudden changes in phase angle as the current varies. Further investigation and analysis are needed. The result from the measurements on the site with EV chargers and a PV inverter is that eight distinct patterns can be observed. Some patterns appear to come from the upstream grid, while some appear to be caused by either one of the EV chargers or the PV inverter, or interaction between them. Further studies are needed. The result from the microgrid measurements is that two distinct patterns at high frequencies (above 1000 Hz) can be observed during grid connected mode and island mode, respectively. During transitions between grid connection and island mode or vice versa, the fundamental frequency varies drastically, and it is therefore hard to analyse potential interharmonics and draw inferences. Further studies are needed. Advantages and disadvantages, as well as ideas for improvements, of the two applied signal processing methods are discussed throughout the different case-studies.

The main subject of this work represents the time-frequency signal analysis. Firstly, it intends to provide the most essential theoretical background with focus on the continuous wavelet transform, where also a comparison of the key features with its close relative the short-time Fourier transform is performed. Afterwards, there follows a demonstration of the purpose with a practical example. The particular aim is to create a phase vocoder solution for modifying the length of a sound record duration and pitch shifting. Here, in this place, the functional principles, design, procedure of assembling, outputs and achieved results are well documented.

The early diagnosis of CP (Cerebral Palsy) in infants is important for developing meaningful interventions. One of the major symptoms of the CP is lack of the coordinated movements of a baby. The bilateral coordinated movement (BCM) is that a baby shows in the early development stage. Each limb movement shows various ranges of speed and angle with fluency in a normal infant. When a baby has CP the movements are cramped and more synchronized. A quantitative method is needed to diagnose the BCM. Data is collected from 3-axis accelerometers, which are connected, to each limb of the baby. Signal processing the collected data using short time Fourier transforms, along with the formation of time-dependent transfer functions and the coherence property is the key to the diagnostic approach. Combinations of each limb's movement and their relationship can represent the correlated movement. Data collected from a normal baby is used to develop the technique for identifying the fidgety movement. Time histories and the resulting diagnostic tool are presented to show the regions of the described movement. The evaluation of the transduction approach and the analysis is discussed in detail. The application of the quantitative tool for the early diagnosis of CP offers clinicians the opportunity to provide interventions that may reduce the debilitating impact this condition has on children. Tools such as this can also be used to assess motor development in infants and lead to the identification and early intervention for other conditions. / Master of Science

Στα πλαίσια της διπλωματικής εργασίας αναπτύχθηκε μία εφαρμογή, η οποία προσδιορίζει τις τρεις πρώτες συχνότητες συντονισμού της φωνητικής κοιλότητας κατά τη διαδικασία της φώνησης φωνηέντων. Οι τρεις αυτές συχνότητες παρέχουν επαρκή πληροφορία για τον προσδιορισμό του φωνήεντου. Η φώνηση εξομοιώνεται με σήμα εισόδου το οποίο παρουσιάζει κορυφές σε αναμενόμενες περιοχές συχνοτήτων. Ο προσδιορισμός των συχνοτήτων συντονισμού στηρίζεται στη μέθοδο βραχύχρονης ανάλυσης Fourier. Η εφαρμογή αναπτύχθηκε σε περιβάλλον μVision της Keil, σε γλώσσα προγραμματισμού C, για τον μικροελεγκτή STM32F103RB της ST Microelectronics. / In the context of this thesis an application was developed, that is capable of estimating the first three formant frequencies (resonances of the vocal tract) in the event of voicing of vowels. These three frequencies provide us enough information to determine the vowel that is voiced. The human voice is being emulated by an input signal which has peaks in the anticipated frequency regions. The formant frequencies are being estimated based on the short-time Fourier analysis method. The application was developed in Keil μVision programming suite, in C programming language, for the STM32F103RB microcontroller by ST Microelectronics.

Le fuseau mitotique assure la ségrégation des chromatides sœurs et le maintien de la poïdie des cellules filles. Le fuseau est composé de microtubules dynamiques (qui polymérisent et dépolymérisent continuellement), de nombreux moteurs moléculaires, d'agents de réticulations et de régulateurs. Bien que la structure du fuseau au niveau moléculaire soit connue, son fonctionnement reste délicat à comprendre, et nécessite la prise en compte de la dynamique de ses composants et leurs interactions. Les approches utilisées pour répondre à ces problématiques sont jusqu'à maintenant plutôt des approches in silico et in vitro. Il manque aujourd'hui une caractérisation de la mécanique du fuseau dans son contexte physiologique. Nous proposons une méthode non invasive basée sur de l'analyse d'image, combiné à une modélisation heuristique pour mesurer les paramètres mécaniques durant toute la division. Nous suivons les pôles du fuseau marqués par protéine fluorescente avec un taux acquisition rapide et une bonne résolution spatiale ce qui nous permet d'accéder aux fluctuations de longueur du fuseau in vivo. Avec la transformée de Fourier aux temps courts, nous calculons leurs densités spectrales de puissances — leurs signatures mécaniques. Ces spectres sont alors ajustés avec un modèle Kelvin — Voigt avec inertie (un ressort, un amortisseur et un terme inertiel en parallèle). Nous avons validé la méthode par des expériences numériques où nous retrouvons les évolutions des paramètres sur des données simulées et la calibration a été réalisée par l'utilisation de la rupture du fuseau induite par micro chirurgie laser ou par la génétique. Nous avons caractérisé le fuseau de l'embryon unicellulaire du nématode C. elegans. La méthaphase apparaît dominée par l'amortisseur, ce qui est cohérent avec la lente élongation du fuseau que nous observons. Mais contraste l'idée répandue de l'existence d'un mécanisme de maintien de la longueur du fuseau durant la métaphase. Au passage en anaphase, les trois paramètres mécaniques chutent, avant de réaugmenter environ 50 secondes après la transition pour réatindre un régime dominé de nouveau par l'amortisseur, ce qui suggère que les microtubules interpolaires jouent un rôle mineur durant l'élongation du fuseau en début d'anaphase. Dans la perspective de comprendre le lien entre la mécanique du fuseau et les interactions des acteurs moléculaires, nous avons partiellement supprimé un gène par sous-structure du fuseau. Nous avons alors retrouvé des comportements connus avec une perspective augmentée offerte par notre méthode. Cette méthode, ne va pas seulement permettre la compréhension fondamentale de la mécanique du fuseau, en remplaçant la modélisation du fuseau basé uniquement sur la longueur, mais aussi d'aller vers la prise en compte de la robustesse de fonctionnement du fuseau mitotique face aux défauts tel que la polyou l'aneuploïdie. / The mitotic spindle ensures the correct segregation of the sister chromatids to maintain ploidy in daughter cells. The spindle comprises dynamical microtubules (alternating polymerizing and depolymerizing), a variety of molecular motors, crosslinker and the regulators. Although the molecular grounds of spindle structure is well known, the link to its functions remain elusive, calling for including the dynamics of its components and their interactions. These questions were mostly investigated by in silico or in vitro approaches. But a detailed characterizing of spindle mechanics, in physiological conditions, is missing. We propose an image processing based, non invasive, method combined to an heuristic model to measure mechanical parameters of the mitotic spindle along time. We tracked fluorescently labeled spindle pole at high temporal and spatial resolution and measured the variations of spindle length, in vivo. We computed their power density spectrum using short time Fourier transform (sliding window) — a blueprint of spindle mechanics. Such a spectrum is then fitted with a Kelvin —Voigt model with inertia (a spring, a damper, an inertial element in parallel). We validated this method by recovering the mechanical parameters over time from simulated data and calibrated it uses laser and genetically induced spinlde cut. We characterized the mitotic spindle of the one-cell embryo of nematode C. elegans. Metaphase appeared dominated by damping element, consistent with the slow spindle elongation observed. But in contrast with the common thought that a mechanism maintains the spindle length during metaphase. At anaphase onset, all three parameters collapsed, before increasing about 50s later to reach a regime where damping dominated again, suggesting the overlapping spinlde microtubules may play a minor role in early anaphase spinlde elongation. In perspective of understanding how spindle mechanics emerge of molecular players interactions, we depleted one gene per splindle sub-structure — overlapped microtubules, kinetochore microtubules, central spindle and astral microtubules. We succefully recovered some known behavior but with the augmented insight offered by our method. This method paves the way not only towards understanding the fundamentals of spindle mechanics, superseding the degenerated modeling based on the sole spindle length but also towards acounting for spindle functional robustness towards defect as polyor aneuploidy.

This thesis study focuses on the realization of an online cue based Motor Imagery (MI) Brain Computer Interface (BCI). For this purpose, some signal processing and classification methods are investigated. Specifically, several time-spatial-frequency methods, namely the Short Time Fourier Transform (STFT), Common Spatial Frequency Patterns (CSFP) and the Morlet Transform (MT) are implemented on a 2-class MI BCI system. Distinction Sensitive Learning Vector Quantization (DSLVQ) method is used as a feature selection method. The performance of these methodologies is evaluated with the linear and nonlinear Support Vector Machines (SVM), Multilayer Perceptron (MLP) and Naive Bayesian (NB) classifiers. The methodologies are tested on BCI Competition IV dataset IIb and an average kappa value of 0.45 is obtained on the dataset. According to the classification results, the algorithms presented here obtain the 4th level in the competition as compared to the other algorithms in the competition. Offline experiments are performed in METU Brain Research Laboratories and Hacettepe Biophysics Department on two subjects with the original cue-based MI BCI paradigm. Average prediction accuracy of the methods on a 2-class BCI is evaluated to be 76.26% in these datasets. Furthermore, two online BCI applications are developed: the ping-pong game and the electrical wheelchair control. For these applications, average classification accuracy is found to be 70%. During the offline experiments, the performance of the developed system is observed to be highly dependent on the subject training and experience. According to the results, the EEG channels P3 and P4, which are considered to be irrelevant with the motor imagination, provided the best classification performance on the offline experiments. Regarding the observations on the experiments, this process is related to the stimulation mechanism in the cue based applications and consequent visual evoking effects on the subjects.

Commercial Aircraft Corporation of China, Ltd (COMAC)and Chinese Scholarship Council. / The aim of this thesis is to develop a real time PIO detection and mitigation system that consists of a detector based on short time Fourier transform(STFT) and autoregressive model(ARX) with exogenous inputs, together with an adaptive controller based mitigation system. The system not only detects the traditional PIO characteristics but also focuses on the trend of pilot behaviour by calculating the rate of change in the open loop crossover frequency. In the detection system, a sliding windowed STFT method was applied to identify the frequency and phase characteristics of the system via processing the signal of pilot input and aircraft state. An ARX model was also applied to get the rate of change of the crossover frequency. After detection, a PIO cue was shown on the primary flight display. A scheduled gain controller was coupled to provide PIO mitigation by varying stick input gain. Compensatory and tracking tests for the evaluation of this system were performed using a quasi-linear Boeing-747 aircraft model including nonlinear command gearing and actuator rate-limiting. Bandwidth and Gibson criteria were used to design PIO prone control laws for system evaluation experiments. Results from PIO tests conducted on desktop PCs were presented. These were analyzed and compared with those obtained from implementing the Real-time Oscillation Verifier module available in literature.

Extracting the Electric Network Frequency (ENF) fluctuations from an audio recording and comparing it to a reference database is a new technology intended to perform forensic digital audio authentication. The objective of this thesis is to implement and design a range of programs and algorithms for capturing and extracting ENF signals. The developed C-program combined with a probe can be used to build the reference database. Our implementation of the Short-Time Fourier Transform method is intended for the ENF extraction of longer signals while our novel proposed use of the Autoregressive parametric method and our implementation of the zero-crossing approach tackle the case of shorter recordings. A Graphical User Interface (GUI) was developed to facilitate the process of extracting the ENF fluctuations. The whole process is tested and evaluated for various scenarios ranging from long to short recordings.