Submitted by Luciana Ferreira (lucgeral@gmail.com) on 2015-05-05T12:18:03Z No. of bitstreams: 2 Dissertação - Jhonathan Gonçalves da Rocha - 2014.pdf: 2415674 bytes, checksum: 4a8413e486c281e025f18b39fa5ad0c3 (MD5) license_rdf: 21267 bytes, checksum: 73e23c2acaaf13389e092bd813e3223d (MD5) / Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2015-05-05T12:20:02Z (GMT) No. of bitstreams: 2 Dissertação - Jhonathan Gonçalves da Rocha - 2014.pdf: 2415674 bytes, checksum: 4a8413e486c281e025f18b39fa5ad0c3 (MD5) license_rdf: 21267 bytes, checksum: 73e23c2acaaf13389e092bd813e3223d (MD5) / Made available in DSpace on 2015-05-05T12:20:02Z (GMT). No. of bitstreams: 2 Dissertação - Jhonathan Gonçalves da Rocha - 2014.pdf: 2415674 bytes, checksum: 4a8413e486c281e025f18b39fa5ad0c3 (MD5) license_rdf: 21267 bytes, checksum: 73e23c2acaaf13389e092bd813e3223d (MD5) Previous issue date: 2014-09-01 / Fundação de Amparo à Pesquisa do Estado de Goiás - FAPEG / Leishmaniasis is neglected infectious and parasitic diseases caused by protozoa of the genus Leishmania, having different clinical forms according to the type of host-parasite relationship established, the main ones being: Leishmaniasis Visceral and Cutaneous Leishmaniasis, object of this study. The fact that conventional treatments present limitations and difficulties for patients coupled with increasing use of low level laser therapy in the treatment of skin lesions of various etiologies, specifically with Low power laser, led us to evaluate the possible consequences of such treatment in American cutaneous leishmaniasis in both murine experimental models (in vivo), with the aim of evaluating the dynamics of healing of ulcerated lesions, as in cultured parasites (in vitro), with the use of techniques that allowed us to evaluate the interference of therapy in cultures of the parasites isolated from the MAB-6 Leishmania (Leishmania) amazonensis Leishmanias belonging to the Bank of the Midwest, with regard to the ability to multiply, viability, integrity and infective capacity. The tests "in vivo" concluded that the use of low-power laser is an interesting tool in the treatment of ulcerative lesions caused by cutaneous leishmaniasis since it leads to decreased edema, stimulates microbicidal action of macrophages and promotes the maturation of collagen. Already in essays "in vitro", it was found that the action of the treatment leads to a static effect on the growth curve of the parasites. The viability tests showed a small increase in the number of parasites killed after treatment and reduction in mitochondrial activity. The low level laser therapy proved incapable of promoting the apoptotic process and modify the composition of the plasma membrane of the parasite, which suggests that the laser treatment did not affect the ability of infection. Instead, there was a rise in titers of viable parasites present in the paws of mice infected with Leishmania treated. / As leishmanioses são doenças infecto-parasitárias negligenciadas, causadas por protozoários do gênero Leishmania, possuindo diferentes formas clínicas de acordo com o tipo de relação parasito-hospedeiro estabelecida, sendo as principais: Leishmaniose Visceral e Leishmaniose Tegumentar Americana, objeto deste estudo. O fato dos tratamentos convencionais apresentarem limitações e dificuldades aos pacientes aliado ao crescente uso da laserterapia de baixa potência no tratamento de lesões dermatológicas de diferentes etiologias, especificamente com o Laser de baixa potência, levou-nos à avaliação das possíveis consequências deste tipo de tratamento na leishmaniose tegumentar americana, tanto em modelos experimentais murinos (in vivo), com o objetivo de avaliar a dinâmica da cicatrização das lesões ulceradas, quanto em culturas de parasitos (in vitro), com o emprego de técnicas que permitiram avaliar a interferência da terapia nas culturas dos parasitos provenientes do isolado MAB-¨6 de Leishmania (Leishmania) amazonensis pertencentes ao Banco de Leishmanias do Centro-Oeste, no que se refere à capacidade de multiplicação, viabilidade, integridade e capacidade infectante. Os ensaios “in vivo” permitiram concluir que o uso do laser de baixa potência é uma interessante ferramenta no tratamento das lesões ulceradas provocadas pela leishmaniose tegumentar americana uma vez que leva à diminuição do edema, estimula a ação microbicida dos macrófagos e favorece a maturação do colágeno. Já nos ensaios “in vitro”, foi verificado que a ação do tratamento leva a um efeito estático sobre a curva de crescimento dos parasitos. Os testes de viabilidade mostraram um pequeno aumento no número de parasitos mortos após o tratamento e na atividade redutora mitocondrial. A laserterapia de baixa potência mostrou-se incapaz de favorecer o processo apoptótico e de modificar a composição da membrana plasmática do parasito, o que permite supor que tratamento com o laser não prejudica a capacidade de infecção. Ao invés disso, verificou-se uma elevação nos títulos de parasitos viáveis presentes nas patas dos camundongos infectados com leishmânias tratadas.

Yes / Soft silicone's flexibility, adhesive capacity and non-toxic, non-odourous and hypoallergenic nature have made it an established material for adhesive and protective therapeutic devices. In wound care, silicone is a component of contact layer dressings for superficial wounds and silicone gel sheeting for reducing the risk of scarring, as well as of barriers for incontinence-associated dermatitis. Regarding stoma accessories, silicone is established in barrier films to prevent contact dermatitis, adhesive removers to prevent skin stripping and filler gels to prevent appliance leaks. Until recently, silicone has not been used in stoma appliances flanges, as its hydrophobic nature has not allowed for moisture management to permit trans-epidermal water loss and prevent maceration. Traditional hydrocolloid appliances manage moisture by absorbing water, but this can lead to saturation and moisture-associated skin damage (MASD), as well as increased adhesion and resultant skin tears on removal, known as medical adhesive-related skin injury (MARSI). However, novel silicone compounds have been developed with a distinct evaporation-based mechanism of moisture management. This uses colloidal separation to allow the passage of water vapour at a rate equivalent to normal trans-epidermal water loss. It has been shown to minimise MASD, increase wear time and permit atraumatic removal without the use of adhesive solvents. Trio Healthcare has introduced this technology with a range of silicone-based flange extenders and is working with the University of Bradford Centre for Skin Sciences on prototype silicone-based stoma appliance flanges designed to significantly reduce the incidence of peristomal skin complications, such as MARSI and MASD. It is hoped that this will also increase appliance wear time, reduce costs and improve patient quality of life.