SKIP and BIR/Survivin are evolutionarily conserved proteins. SKIP is a known transcription and splicing cofactor while BIR-1/Survivin regulates cell division, gene expression and development. Loss of function of C. elegans SKIP (SKP-1) and BIR-1 induces overlapping developmental phenotypes. In order to uncover the possible interactions of SKP-1 and BIR-1 on the protein level, we screened the complete C. elegans mRNA library using the yeast two-hybrid system. These experiments identified partially overlapping categories of proteins as SKP-1 and BIR-1 interactors. The interacting proteins included ribosomal proteins, transcription factors, translation factors and cytoskeletal and motor proteins suggesting involvement of the two studied proteins in multiple protein complexes. To visualize the effect of BIR-1 on the proteome of C. elegans we induced a short time pulse BIR-1 overexpression in synchronized L1 larvae. This led to a dramatic alteration of the whole proteome pattern indicating that BIR-1 alone has the capacity to alter the chromatographic profile of many target proteins including proteins found to be interactors in yeast two hybrid screens. The results were validated for ribosomal proteins RPS-3, RPL-5, non-muscle myosin and TAC-1, a transcription cofactor and a centrosome associated...

Accurately delivering and precisely timing sprinkler irrigation improves peanut yield and profitability, but there are no data on how to achieve this in the mid-southern USA where furrow-irrigation dominates. This research was conducted to determine if soil water potential could be manipulated through land preparation method, irrigation delivery, and irrigation scheduling. The effects of land preparation method (flat vs bed), furrow-irrigation delivery (every vs every-other furrow), and irrigation scheduling [Food and Agriculture Organization and drainage paper 56 (FAO-56), - 50 kPa, -75 kPa, and -100 kPa] on peanut yield, net returns above irrigation costs, and irrigation water use efficiency were investigated near Stoneville, MS on a Bosket very fine sandy loam. Our data indicate that regardless of land preparation method, peanut yield, net returns above irrigation costs, and irrigation water use efficiency are most often optimized in the mid-southern USA by irrigating every other furrow at a threshold of -50 kPa.

Radio map estimation (RME) is the task of predicting radio power at all locations in a two-dimensional area and at all frequencies in a given band. This thesis explores four deep learning approaches to RME: dual path autoencoders, skip connection autoencoders, diffusion, and joint learning with transmitter localization.

This study examines the experiences of young adult women raised in non-resident father households in Tlokwe Local Municipality, North West. Focus groups and face- to-face interviews were conducted with 34 young women between the ages 18-25. Five important, nuanced themes emerged, these are: a) fathers who are physically present but emotionally absent, b) fathers who are physically present but uninvolved, c) fathers who are physically absent but involved, d) fathers who are physically present and involved and e) fathers who are absent and uninvolved. Non-resident fatherhood remains a challenge in the Tlokwe Municipality, most of the participants had never met their biological fathers. The study revealed the frustration young women feel towards their mothers and maternal grandparents for caring more about maintenance than they do about a healthy father-daughter relationship. The study further revealed a deep- seated need for close proximity and an involved type of fathering among the young women. / Sociology / M.A.(Sociology)

Magister Curationis / Binary search trees (BSTs) are important data structures which are widely used in various guises. Splay trees are a specific kind of binary search tree, one without explicit balancing. Skip lists use more space than BSTs and are related to them in terms of much of their run-time behavior. Even though binary search trees have been used for about half a century, there are still many open questions regarding their run-time performance and algorith mic complexity. In many instances, their worst-case, average-case, and best-case behaviors are unknown and need further research. Our analysis provides a basis for selecting more suitable data structures and algorithms for specific processes and applications. We contrast the empirical behavior of splay trees and skip lists with their theoretical behavior. In particular we explore when splay trees outperform skip lists and vice versa. The performance of a splay tree depends on the history of accesses to its el ements. On the other hand, the performance of a skip list depends on an indepen dent randomization of the height of links that lead to specific elements. Therefore, probabilistic methods are used to analyze the operation of splay trees and skip lists. Our main results are that splay trees are faster for sorted insertion, where AVL trees are faster for random insertion. For searching, skip lists are faster than single class top-down splay trees, but two-class and multi-class top-down splay trees can behave better than skip lists.

This master’s thesis deals with a strength analysis of the main frame and loading arms of a skip loader. For a calculation of load states is used a dynamic simulation and its results are used as a boundary conditions for the strength analysis using finite element method (FEM). The results of the analysis is evaluated and on theirs basis are designed a construction improvements. The part of the thesis is drawings documentation of the construction improvements.

No description available.

Die Notwendigkeit von Bx42 für Drosophila Entwicklung und seine Beteiligung an unterschiedlichen zellulären Prozessen wurde mit Hilfe von RNA Interference (RNAi) demonstriert. Das ubiquitäre Ausschalten oder die Reduktion der Bx42 Expression mittels RNAi führte dabei zu embryonaler Letalität. Weiterhin führte eine gewebespezifische Induktion von Bx42 in Abhängigkeit der verwendeten Treiberlinien bei unterschiedlichen Temperaturen zu mehreren verschiedenen adulten Phänotypen. Diese Phänotypen waren die Grundlage für die Annahme, dass Bx42 eine Rolle in der Regulation mehrerer verschiedener Zellsignalwege spielt. In der Tat interagiert Bx42 mit den Proteinen des Notch-Signalweges Suppressor of hairless [Su(H)] und Notch intracellular domain (N-IC). Zusätzlich werden bei einer Verminderung von Bx42 die Notch Zielgene cut (ct) und enhancer of split m8 [e(Spl)m8] reprimiert (Negeri et al., 2002). In dieser Arbeit wurde die Beteiligung von Bx42 am TGF-ß/Dpp Signalweg untersucht. Es wurde gezeigt, dass Bx42 mit den TGF-ß/Dpp-Signalweg Proteinen Mad und Medea sowohl in vitro als auch in vivo interagiert. Die dabei verwendeten Methoden waren das Hefe-Zwei Hybrid-Sytem und Ni-NTA-Pulldown-Assays. Domänen der Smad Proteine (Mad und Medea), die für die Interaktion mit Bx42 notwendig sind, wurden mit Hilfe von Deletionskonstrukten untersucht. Es konnte gezeigt werden, dass die stark konservierte MH2-Domäne dieser Proteine für die Interaktion notwendig ist. Zudem belegten Versuche die genetische Interaktion zwischen Bx42 und Medea, in denen ein Bx42-RNAi-Phänotyp durch die gleichzeitige Überexpression von Medea gerettet werden konnte. Es ist bekannt, dass das humane Bx42-Homolog Skip sowohl mit den Proteinen Smad2 und 3 des TGF-ß/Activin Signalweg, als auch mit den Onkogenen Sno und Ski interagiert. Skip wirkt hier als Antagonist der Ski/Sno-Wirkung auf den TGF-ß/Activin-Signalweg und fungiert als Koaktivator (Leong et al., 2001). Die Interaktion zwischen Bx42 und der TGF-ß/Activin-Signalweg Komponente dSmad2, sowie mit dem Onkogen dSno konnte in dieser Arbeit auch für Drosophila bewiesen werden. Die Bedeutung dieser Wechselwirkung muss noch in weiteren Arbeiten analysiert werden. Der Einfluss der Bx42-RNAi-Induktion auf die TGF-ß/Dpp Zielgene distal-less (dll), optomotor blind (omb) und spalt (sal) wurde anhand von Reportergen Untersuchungen mit enhancer-trap-Linien und RNA in situ Hybridisierung untersucht. Es konnte gezeigt werden, dass das Ausschalten von Bx42 die Expression dieser Gene in ähnlicher Weise reprimiert, wie eine Elimination des TGF-ß/Dpp-Signals. Diese Ergebnisse unterstützen die Annahme, dass Bx42 in der Lage ist, TGF-ß/Dpp Zielgene durch eine Wechselwirkung mit Mad und Medea zu aktivieren. / The importance of Bx42 in Drosophila development was demonstrated using Bx42-RNA interference. The ubiquitous downregulation of Bx42 generated embryonic lethality, indicating the importance of this protein in early development. The tissue specific induction of Bx42-RNAi resulted in several different phenotypes depending on the driver line and the temperature at which animals were raised. The phenotypes obtained were the key point for the assumption that Bx42 may play a role in the regulation of a number of different cellular signalling pathways. Indeed, within the Notch signalling pathway Bx42 interacts genetically with Suppressor of hairless [Su(H)] and Notch intracellular domain (N-IC). Additionally, the reduction of Bx42 negatively affected the expression of the Notch target Genes cut (ct) and enhancer of split m8 [e(Spl)m8] (Negeri et al., 2002). In this work, the involvement of Bx42 in the Dpp signalling pathway was investigated. It was shown that Bx42 interacts both in vitro and in vivo, as demonstrated by yeast two hybrid protein-protein studies and Ni-NTA pull-down assays, with the TGF-ß/ Dpp components Mad and Medea. Domains of Smads (Mad and Medea) required for Bx42 interaction were examined using deletion constructs of Smads and the importance of the well conserved MH2 domains of Mad and Medea for this interaction was revealed. Moreover, the rescue of the Bx42-RNAi phenotype by the simultaneous overexpression of Medea demonstrated the genetic interaction between Bx42 and Medea. Furthermore, evidences for the interaction of Bx42 with the TGF-ß/Activin pathway component dSmad2 and with the oncogene protein dSno were obtained from interaction assays. The human homologue of Bx42, Skip, also interacts with Smad2/3 or Sno. The meaning of this interaction in Drosophila has yet to be analysed. The influence of Bx42-RNAi induction on the expression of Dpp target genes distal less (dll), optomotor blind (omb) and spalt (sal) was also investigated using enhancer trap lines and RNA in situ hybridisation. In this way it was proven that these genes are suppressed as they are by elimination of Dpp signalling. These results suggest that Bx42 may be able to modulate positively TGF-ß/Dpp signalling through an interaction with the signalling transducer Mad and Medea.

Submitted by Evandro Lopes (dalbem.evandro@gmail.com) on 2016-04-04T03:14:32Z No. of bitstreams: 1 dissertacao_skip_gram.pdf: 1559216 bytes, checksum: c9487105e0e9341acd30f549c30d4dc9 (MD5) / Approved for entry into archive by Janete de Oliveira Feitosa (janete.feitosa@fgv.br) on 2016-07-19T19:55:35Z (GMT) No. of bitstreams: 1 dissertacao_skip_gram.pdf: 1559216 bytes, checksum: c9487105e0e9341acd30f549c30d4dc9 (MD5) / Approved for entry into archive by Marcia Bacha (marcia.bacha@fgv.br) on 2016-07-25T17:47:32Z (GMT) No. of bitstreams: 1 dissertacao_skip_gram.pdf: 1559216 bytes, checksum: c9487105e0e9341acd30f549c30d4dc9 (MD5) / Made available in DSpace on 2016-07-25T17:47:47Z (GMT). No. of bitstreams: 1 dissertacao_skip_gram.pdf: 1559216 bytes, checksum: c9487105e0e9341acd30f549c30d4dc9 (MD5) Previous issue date: 2015-06-30 / There is a representation problem when working with natural language processing because once the traditional model of bag-of-words represents the documents and words as single matrix, this one tends to be completely sparse. In order to deal with this problem, there are some methods capable of represent the words using a distributed representation, with a smaller dimension and more compact, including some properties that allow to relate words on the semantic form. The aim of this work is to use a dataset obtained by the Media Cloud Brasil project and apply the skip-gram model to explore relations and search for pattern that helps to understand the content. / Existe um problema de representação em processamento de linguagem natural, pois uma vez que o modelo tradicional de bag-of-words representa os documentos e as palavras em uma unica matriz, esta tende a ser completamente esparsa. Para lidar com este problema, surgiram alguns métodos que são capazes de representar as palavras utilizando uma representação distribuída, em um espaço de dimensão menor e mais compacto, inclusive tendo a propriedade de relacionar palavras de forma semântica. Este trabalho tem como objetivo utilizar um conjunto de documentos obtido através do projeto Media Cloud Brasil para aplicar o modelo skip-gram em busca de explorar relações e encontrar padrões que facilitem na compreensão do conteúdo.

This study examines the experiences of young adult women raised in non-resident father households in Tlokwe Local Municipality, North West. Focus groups and face-to-face interviews were conducted with 34 young women between the ages 18-25. Five important, nuanced themes emerged, these are: a) fathers who are physically present but emotionally absent, b) fathers who are physically present but uninvolved, c) fathers who are physically absent but involved, d) fathers who are physically present and involved and e) fathers who are absent and uninvolved. Non-resident fatherhood remains a challenge in the Tlokwe Municipality, most of the participants had never met their biological fathers. The study revealed the frustration young women feel towards their mothers and maternal grandparents for caring more about maintenance than they do about a healthy father-daughter relationship. The study further revealed a deep-seated need for close proximity and an involved type of fathering among the young women. / Sociology / M.A. (Sociology)