Reports and consultative documents published at national level since about 1980 have indicated that British Industry must look to modern technology and also educate and train its workers on a 'broad base', with an 'integrated' approach. Traditionally, and still very much the mode of operation, teaching has been confined within subject boundaries. A research group was established by Professor Bajpai consisting of the author, Mr Rod Bond (Burleigh Community College, Loughborough) and a few others working overseas to investigate a teaching strategy based on an interrelated approach to teaching mathematics. Measurement was chosen as the first topic of investigation using this approach which then formed the basis for further research undertaken by the two research workers of the group whose work is reported in the form of two theses. This thesis aims to show that mathematics is naturally related to science and technology in industrial practice and that when taught in an interrelated way it would be more interesting and have more relevance to real applications in technology-based employment at craft and technician levels. To help establish the case experiments carried out by the author are referred to; these include a few case studies, a questionnaire survey and results analysed from more than five hundred basic mathematics tests. The various kinds of mathematics taught in further education are described and compared with mathematics in a practical context as seen from a case study within an engineering training school. Next a survey of mathematics at work shows that, like the training school, there is a task associated with the mathematics which is also related to science or technology or both. Another case study in the pharmaceutical industry lends further support to the way mathematics is used in industry. Much of the mathematics also seems to be basic and used in association with measurement and a particular task. It was decided by the research group that a tape/slide programme on measurement for students and educators should be developed by the author and tested in different situations. Teaching modules on relevant mathematical topics based on the interrelated approach were constructed for students with strong support from industry in the form of materials and advice. Testing of these modules, in their original and revised forms after feedback, is described. These trials were also carried out in other establishments. Modules based upon the interrelated approach developed by the author formed a basis for promoting the underlying philosophy behind this approach. These were presented to educators in in-service training and staff development programmes in the north western region of the UK with success. Observations and conclusions drawn clearly indicate that this type of method makes mathematics more interesting and relevant for students of different abilities and backgrounds. Finally pointers are given in the thesis as to the wider use and promotion of this approach for teaching mathematics in further education.

L'ADN est au cœur de nombreux processus biologiques. De ce fait, il est la cible de nombreuses molécules pharmacologiques employées notamment dans les thérapies anticancer. Parmi celles-ci, la daunomycine va interagir avec la double hélice d'ADN en s'intercalant entre deux paires de bases, bloquant ainsi la réplication. Malgré l'efficacité reconnue des molécules intercalantes, et de nombreuses études à ce sujet, le mécanisme du processus d'intercalation n'est pas clairement déterminé à l'heure actuelle. Basés sur l'utilisation de la dynamique moléculaire avec une représentation tout atome des systèmes en conditions de solvant explicite, ces travaux ont tout d'abord visé à caractériser l'influence de la flexibilité conformationnelle de la daunomycine vis à vis de l'ADN, ainsi que son importance dans les interactions entre ces deux molécules. Dans un deuxième temps nous nous sommes focalisés sur le chemin réactionnel menant la daunomycine à son site d'intercalation par umbrella sampling. Cette étude, en plus de fournir des énergies libres en accord avec les données expérimentales, a permis de pointer du doigt un mécanisme d'intercalation impliquant une étape préliminaire de liaison de la daunomycine au petit sillon de l'ADN, suivie d'une étape intermédiaire de réorientation du ligand. Finalement, la réalisation de simulations de glissement de la daunomycine le long du petit sillon de l'ADN nous a permis de nous intéresser au mécanisme permettant à ce ligand de localiser son site d'intercalation le long de l'ADN / DNA is at the heart of many biological processes. Therefore, it is the target of numerous pharmacological molecules used in cancer therapies. Among them, daunomycin will interact with the double helix by intercalation between DNA’s base pairs, thus blocking replication. Despite the proven efficiency of intercalating molecules, and many studies on this subject, the mechanism of intercalation process is not yet clearly understood. Based on molecular dynamics with an all atoms representation in explicit solvent conditions, this work, in one hand, aim to characterize the influence of the conformational flexibility of daunomycin during interactions with DNA. In a second hand, we focused on the reaction pathway leading daunomycine to its intercalation site using umbrella sampling. This study, in addition to providing free energies in agreement with experimental data, has allowed to highlight an intercalation mechanism involving a preliminary step where daunomycin bind to the minor groove of DNA, followed by a intermediate step of reorientation of the ligand. Finally, the realization of sliding simulations of daunomycin along DNA minor groove, has allowed us to focus on the mechanism that allow a ligand to locate its intercalation site on DNA

Semantic segmentation as an approach to recognizing and localizing objects within an image is a major research area in computer vision. Now that convolutional neural networks are being increasingly used for such tasks, there have been many improve- ments in grand challenge results, and many new research opportunities in previously untennable areas. Using fully convolutional networks, we have developed a semantic segmentation pipeline for the identification of melanocytic tumor regions, epidermis, and dermis lay- ers in whole slide microscopy images of cutaneous melanoma or cutaneous metastatic melanoma. This pipeline includes processes for annotating and preparing a dataset from the output of a tissue slide scanner to the patch-based training and inference by an artificial neural network. We have curated a large dataset of 50 whole slide images containing cutaneous melanoma or cutaneous metastatic melanoma that are fully annotated at 40× ob- jective resolution by an expert pathologist. We will publish the source images of this dataset online. We also present two new FCN architectures that fuse multiple deconvolutional strides, combining coarse and fine predictions to improve accuracy over similar networks without multi-stride information. Our results show that the system performs better than our comparators. We include inference results on thousands of patches from four whole slide images, reassembling them into whole slide segmentation masks to demonstrate how our system generalizes on novel cases.

The purpose of this Master thesis is to verify migration of mesenchymal stem cells on the principle known as chemotaxis. First part of this study is focused on cell migration in order to explain the whole migration process. Next part describes various chemotaxis methods and selected studies dealing with clinical applications of mesenchymal stem cells in different medical and biomedical fields. The following step describes confocal microscopy, which is used for acquiring images of the cells. The experimental part is focused on cultivation of mesenchymal stem cells in a laboratory, which is necessary for cell vitality. Furthermore, there are designed two main experiments. Firstly there is a 2D experiment with adherent cells for chemotaxis using -Slide Chemotaxis. Secondly Transwell migration test is designed and executed. Finally, the acquired images from confocal microscope are used for image processing, which was done in Matlab R2020a programming environment. The result of this processing is evaluation of cell confluence and migration. In the end, experimental part of this study was optimized according to recommended studies. The results are summarized in the conclusion with proposal for improvements of those methods.

This diploma thesis deals with the design and optimization of a headstock for machine tool. Thesis was focused to comparison of several types of slide-ways and linear guides of headstock, considering their static and dynamic rigidity. An overview in the field of machine tools, their static and dynamic properties, use materials and design of headstocks, considering types of guideways, types of guideways drives and types of drives of spindle was made. Among the other main goals is included design of several types of guideway of headstock for horizontal machine tool, elaboration of computational models and evaluation of results.

Subject of this diploma thesis is manufacturing of the guiding rail HB 02150 made of metal sheets and used for table slide rail in drawer system. This thesis was made in cooperation with Hettich ČR Company which is producer of furniture hardware. Theses contains theoretical part which explains matters of cutting and bending technology. Marginally will be mentioned matters of spot welding. Practical part contains explanation of production of guiding rail, lay-out of bending roll and cutting tools. In this work there is also described solution of problem with wastrels caused by faulty welding of metal sheets and the draft of welding fixture which solves this problem. Economical and technical summary of this manufacturing process is the last part of this thesis.

This diploma work is dealing with the progressive production technology proposal of the valve body type S43 components. This is the high-pressure valve for the classical power supply. Here in the work is described the structural design of the valves, the material of the valve body and its machinability. Following an analysis of the existing production technology is the new production technology proposal for this part. All is designed with regards to the business possibilities of the Armaturka Vranová Lhota company. In conclusion of the work is presented the technical economic evaluation of both the existing and the proposed manufacturing technology.

This thesis deals with the control of grid of lift machine and rigid hanging of ropes for elevator with a capacity of 675 kg. The main focus is strength analysis using finite element method. Furthermore, a calculation was performed also for anchoring of slide-ways and strength check was done according to standard ČSN EN 81-1. The thesis contains also the assembly drawing of grid of lift machine and it was executed in cooperation with LIFTMONT CZ s.r.o.

The aim of the diploma thesis is to design the technical solution of minimization of frictional losses, which occur when using a sine pump at position of the shaft locking and on the impeller wheel. The losses originating from the frictional forces have a negative influence on the lifetime of individual parts and economy of the pump. In the first part the author describes sine pumps from the constructional point of view and the main producers are mentioned. The second part contains suggestions on the parameters of the pump and various possibilities of solutions. The last part covers the experiment and conclusions.

The Master thesis deals with the possible production of the plastic unit with thread. As the most suitable material was chosen the polyformaldehyde (POM), its production label is Delrin 500P NC010 by Du Pont. The thesis consists of a part with the information about the technology of injection molding of plastics and a part where processing parameters for the production of the plastic unit are designed. Part of the solution was to create an assembly design of injection mold. Since the plastic unit with thread is very specificly shaped the form is handled as a slide mold. The mold is pieced together from normalized parts by the Hasco and DME company. The serie of 100 000 pieces would be delivered in 5 months. Because of the cost savings a solution of the cold-runner system was suggested. A suitable injection molding machine was chosen from the calculated values from Arburgfroms – Type 370s Allrounder 500-170.At the end of the thesis costs were evalueted for acquire per one piece of adapter.