Orientador: Ronaldo da Silva Viana / Resumo: A cultura do sorgo é de grande importância para o país, principalmente por propiciar várias formas de utilização, destacando-se a alimentação humana e de animais, produção de energia renovável, além da alta resistência a seca e outros fatores climáticos adversos. Assim, objetivou-se avaliar a aplicação de doses de fósforo (P) e de Ethephon no desenvolvimento, produtividade, qualidade tecnológica e bromatológica dos cultivares de sorgo Silotec 20 (dupla aptidão) e BRS 508 (sacarino). Os experimentos foram conduzidos na Fazenda de Ensino, Pesquisa e Extensão-FEPE, localizada no município de Selvíria-MS. O solo da área experimental é classificado como LATOSSOLO VERMELHO distrófico, textura argilosa. O delineamento experimental foi blocos ao acaso, em esquema fatorial 5x4, sendo cinco doses de fósforo (0, 40, 80, 120 e 160 kg ha-1 de P2O5), utilizando como fonte, superfosfato simples e quatro doses do Ethephon (0, 330, 660 e 1.320 mL ha-1), com quatro repetições. As variáveis analisadas no sorgo Silotec 20 e BRS 508 foram: altura de plantas, diâmetro do colmo, stand aos 15 e 60 dias após a aplicação (D.A.A) de Ethephon, volume de biomassa fresca e seca, produtividade e análise de macronutrientes. Além dessas, foram avaliadas no sorgo Silotec 20 as variáveis bromatológicas: matéria seca (MS), cinzas (CZ), extrato etéreo (EE), fibra em detergente neutro (FDN), fibra em detergente ácido (FDA), celulose (CEL) e lignina (LIG). No sorgo BRS 508 foram analisadas as variáveis tecnológica... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Sorghum crop have great importance for the country, mainly because it provides various forms of use, including human and animal nutrition, renewable energy production, as well as high resistance to drought and other adverse climatic factors. Thus, the objective of this study was to evaluate the application of phosphorus (P) and Ethephon doses in the development, productivity, technological and bromatological quality of sorghum cultivars Silotec 20 (double suitability) and BRS 508 (saccharin). The experiments were conducted at the Teaching, Research and Extension Farm-FEPE, located in Selvíria-MS. The soil of the experimental area is classified as OXISOL, clay texture. Both experimental design was randomized blocks in a 5x4 factorial scheme, with five phosphorus doses (0, 40, 80, 120 and 160 kg ha-1 of P2O5), using simple superphosphate as source and four doses of Ethephon (0, 330, 660 and 1,320 mL ha-1), with four replications. The variables analyzed in Silotec 20 and BRS 508 sorghum were: plant height, stem diameter, stand at 15 and 60 days after Ethephon application (D.A.A), fresh and dry biomass volume, yield and macronutrient analysis. In addition, it were evaluated in sorghum Silotec 20 the bromatological variables: dry matter (MD), ash (AH), ether extract (EE), neutral detergent fiber (FDN), acid detergent fiber (FDA), cellulose (CEL), lignin (LIG). And in sorghum BRS 508 were analyzed: the technological variables: soluble solids content (BRIX%); sucrose content (%); re... (Complete abstract click electronic access below) / Mestre

Submitted by Patrícia Costa Silva (patypcs@yahoo.com.br) on 2018-07-30T19:35:52Z No. of bitstreams: 1 Tese_Patricia_Costa_Silva_2018_final.pdf: 1846241 bytes, checksum: b3112d63566f4acc8bf90ba51cfb28d2 (MD5) / Approved for entry into archive by Ana Lucia de Grava Kempinas (algkempinas@fca.unesp.br) on 2018-07-30T20:54:23Z (GMT) No. of bitstreams: 1 silva_pc_dr_botfca.pdf: 1846241 bytes, checksum: b3112d63566f4acc8bf90ba51cfb28d2 (MD5) / Made available in DSpace on 2018-07-30T20:54:23Z (GMT). No. of bitstreams: 1 silva_pc_dr_botfca.pdf: 1846241 bytes, checksum: b3112d63566f4acc8bf90ba51cfb28d2 (MD5) Previous issue date: 2018-05-14 / O cultivo de sorgo sacarino surgiu como alternativa para produção de massa destinada à fabricação de etanol, na entressafra, e em áreas de reforma do canavial e em áreas de rotação à outras culturas. Ainda são escassos estudos sobre a irrigação em sorgo sacarino, bem como os estudos que correlacionam lâminas de irrigação e a dinâmica dos nutrientes no solo e propriedades físicas. Este estudo teve como objetivos analisar o efeito da aplicação de lâminas de irrigação sob características tecnológicas e agronômicas de variedades de sorgo sacarino e nas propriedades do solo, e avaliar a eficiência do uso da água. O experimento foi conduzido em campo na área experimental da Universidade Estadual de Goiás, Câmpus de Santa Helena de Goiás. O delineamento adotado foi o de blocos casualizados com esquema fatorial 5x3, com 5 tratamentos, 3 cultivares de sorgo sacarino e 4 repetições. Os tratamentos foram constituídos por 5 lâminas de irrigação: 25, 50, 75, 100 e 125 % da evapotranspiração de cultura (ETc) e as variedades avaliadas foram BRS 511, CVSW 80007 e Silotec 20. O sistema de irrigação empregado foi o localizado por gotejamento. Os dados foram submetidos à análise de variância pelo teste F a 5% de significância. As médias para o fator variedades e propriedades do solo foram comparadas pelo teste de Tukey e para o fator lâminas de irrigação empregou-se a análise de regressão. Verificou-se que as lâminas de irrigação exerceram influência significativa nas características agronômicas e tecnológicas. A variedade BRS 511 adaptou-se melhor às condições edafoclimáticas locais com reflexo em maior altura de plantas, diâmetro de colmo, número de folhas e internódios, porcentagem de folhas, produtividade de colmos, massa verde da parte aérea, rendimento de caldo, produtividade de etanol e sólidos solúveis totais; e menor peso do bagaço úmido e teor de fibra industrial. Comparando-se as variedades em cada lâmina verificou-se variação a partir da lâmina 75% de reposição da evapotranspiração de cultura com ajustes linear e quadrático. A eficiência do uso da água (EUA) em todas as variedades e lâminas estudadas apresentou resposta linear decrescente, com maior EUA na lâmina de 25% da ETc. A variedade BRS 511 foi a mais recomendada para as condições edafoclimáticas da região avaliada e a lâmina mais indicada foi a equivalente a 100% da ETc. As lâminas de irrigação exerceram influência sobre a dinâmica das propriedades químicas no solo. Os maiores teores dos nutrientes foram encontrados na camada de 0-0,10 m, à medida que aumentou a disponibilidade hídrica; para as lâminas de 100 e 125 % da ETc houve aumento dos teores de nutrientes e de matéria orgânica do solo; entre as variedades e as camadas de solo houve variação somente para o potássio e o alumínio. As propriedades físicas não foram afetadas pelo cultivo das variedades e lâminas de irrigação, apenas pelas camadas amostradas. / Cultivation of sorghum emerged as an alternative for the production of mass destined to the manufacture of ethanol, in the off season, and in areas of reforestation of cane fields and in areas of rotation to other crops. There are still few studies on irrigation in sorghum, as well as studies that correlate irrigation slides and the dynamics of soil nutrients and physical properties. The objective of this study was to analyze the effect of the application of irrigation slides under the technological and agronomic characteristics of sorghum varieties and soil properties, and to evaluate the efficiency of water use. The experiment was conducted in the experimental area of the State University of Goiás, Campus of Santa Helena de Goiás. The experiment was a randomized complete block design with 5x3 factorial, with 5 treatments, 3 sorghum cultivars and 4 replicates. The treatments consisted of 5 irrigation slides: 25, 50, 75, 100 and 125% of crop evapotranspiration (ETc) and the varieties evaluated were BRS 511, CVSW 80007 and Silotec 20. The irrigation system was located by drip. The data were submitted to analysis of variance by the F test at 5% of significance. The averages for the factor varieties and soil properties were compared by the Tukey test and for the irrigation lamina factor the regression analysis was used. It was verified that the irrigation slides exerted significant influence on the agronomic and technological characteristics. The BRS 511 variety was better adapted to local edaphoclimatic conditions with higher plant height, stem diameter, number of leaves and internodes, leaf percentage, shoot yield, shoot shoot mass, broth yield, seed yield ethanol and total soluble solids; and lower weight of the wet cake and industrial fiber content. Comparing the varieties on each slide, a variation was observed from the 75% replacement blade of the crop evapotranspiration with linear and quadratic adjustments. Efficiency of water use in all varieties and slides studied presented a linear decreasing response, with a higher USA in the 25% ETc blade. The BRS 511 was the most recommended for the soil and climatic conditions of the evaluated region and the most indicated blade was the equivalent to 100% of the ETc. Irrigation slides influenced the dynamics of soil chemical properties. The highest levels of nutrients were found in the 0-0.10 m layer, as water availability increased; for the slides of 100 and 125% of the ETc there was an increase in nutrient and soil organic matter contents; between varieties and soil layers there was variation only for potassium and aluminum. The physical properties were not affected by cultivation of the irrigation varieties and slides, only by the sampled layers.

Philosophiae Doctor - PhD / Drought is the most complex phenomenon that remained to be a potential and historic challenge to human welfare. It affects plant productivity by eliciting perturbations related to a pathway that controls a normal, functionally intact biological process of the plant. Sorghum (Sorghum bicolor (L.) Moench), a drought adapted model cereal grass is a potential target in the modem agricultural research towards understanding the molecular and cellular basis of drought tolerance. This study reports on the genomic and proteomic findings of drought tolerance in sorghum combining the results from in silica and experimental analysis. Pipeline that includes mapping expression data from 92 normalized cDNAs to genomic loci were used to identify drought tolerant genes. Integrative analysis was carried out using sequence similarity search, metabolic pathway, gene expression profiling and orthology relation to investigate genes of interest. Gene structure prediction was conducted using combination of ab initio and extrinsic evidence-driven information employing multi-criteria sources to improve accuracy. Gene ontology was used to cross-validate and to functionally assign and enrich genes. An integrated approach that subtly combines functional ontology based semantic data with expression profiling and biological networks was employed to analyse gene association with plant phenotypes and to identify and genetically dissect complex drought tolerance in sorghum. The gramene database was used to identify genes with direct or indirect association to drought related ontology terms in sorghum. Where direct association for sorghum genes were not available, genes were captured using Ensemble Biomart by transitive association based on the putative functions of sorghum orthologs in closely related species. Ontology mapping represented a direct or transitive association of genes to multiple drought related ontology terms based on sorghum specific genes or orthologs in related species. Correlation of genes to enriched gene ontology (GO)-terms (p-value < 0.05) related to the whole-plant structure was used to determine the extent of gene-phynotype association across-species and environmental stresses.

The present work aims to determine the physiological and sanitary quality of Sweet sorghum (Sorghum bicolor (L.) Moench) seeds, as well as to identify pathogens associated with seed, their transmission to seedlings and the subsequent pathogenicity of isolates obtained, In addition, molecularly identify the fungal species pathogenic to this crop. The experiments were carried out in the Teaching and Seed Research Laboratory (TSRL), of the Plant Engineering Department; In the Elocy Minussi Phytopathology Laboratory, Department of Plant Protection, and at the Biological Institute of São Paulo. Sweet sorghum seeds were used, all without chemical treatment. Sanitary quality was evaluated by sanity test, and physiological characteristics by germination and vigor tests (seedling length, dry mass, emergence, rate of emergence and accelerated aging). It was performed the test of transmission of the pathogens associated to the seeds and the subsequent pathogenicity of the obtained isolates, culminating with the molecular characterization of the identified pathogens, in which were sequenced the Internal Transcribed Spacer (ITS) genomic regions and the Elongation Factor 1 - alpha (TEF1-α) gene. The design used was the completely randomized design, with four cultivars of Sweet sorghum (BRS 506, F19, BRS 511 and BRS 509); For the evaluation of pathogenicity, the factorial scheme is represented by four cultivars and three isolates of Fusarium spp., besides the witness. The seeds of the BRS 509 cultivar were considered to have lower physiological quality than the other cultivars. The DNA sequencing allowed identifying the Fusarium thapsinum species as a pathogenic agent in the sweet sorghum crop, and proven its transmission via seeds. / O presente trabalho teve como objetivo determinar a qualidade fisiológica e sanitária de sementes de cultivares de sorgo sacarino (Sorghum bicolor (L.) Moench), bem como identificar os patógenos associados à semente, sua transmissão às plântulas e a posterior patogenicidade de isolados obtidos, além disso, identificar molecularmente as espécies fúngicas patogênicas a esta cultura. Os experimentos foram realizados no Laboratório Didático e de Pesquisas em Sementes (LDPS), do Departamento de Fitotecnia; no Laboratório de Fitopatologia Elocy Minussi, do Departamento de Defesa Fitossanitária e, no Instituto Biológico de São Paulo. Foram utilizadas sementes de sorgo sacarino, todas sem tratamento químico. A qualidade sanitária foi avaliada pelo teste de sanidade, e as características fisiológicas por meio dos testes de germinação e de vigor (comprimento de plântulas, massa seca, emergência, índice de velocidade de emergência e envelhecimento acelerado). Foi realizado o teste de transmissão dos patógenos associados à semente e a posterior patogenicidade dos isolados fúngicos obtidos, culminando com a caracterização molecular dos patógenos identificados, na qual foram sequenciadas as regiões genômicas Internal Transcribed Spacer (ITS) e o gene do fator de elongação 1-α (TEF1α). O delineamento utilizado foi o inteiramente casualizado com quatro cultivares de sorgo sacarino (BRS 506, Fepagro 19, BRS 511 e BRS 509); já para a avaliação da patogenicidade, o esquema fatorial foi representado pelas quatro cultivares e três isolados de Fusarium sp., além da testemunha. As sementes da cultivar BRS 509 foram consideradas de qualidade fisiológica inferior as demais cultivares. O sequenciamento de DNA permitiu identificar a espécie Fusarium thapsinum como agente patogênico na cultura do sorgo sacarino, sendo comprovada sua transmissão via sementes.