Global ETD Search

1	Organ and primary culture of medaka (Oryzias latipes) testis: Test systems for the analysis of cell proliferation and differentiation / Organ und Primärzellkultur von Medaka Testis: Test Systeme zur Untersuchung des Zellproliferation und Zelldifferenzierung Song, Miyeoun 22 June 2003 (has links) (PDF) In cultured medaka testis fragments, cells remained viable for the entire culture period (17h), and spermatids that developed from spermatocytes were viable and motile. Primary cultures were characterized over a period of two days with respect to cell viability and the distribution of adherent and suspended cells. These two cell populations were maintained in dynamic equilibrium in vitro for several days. Proliferating cells were predominant among clusters of suspended cells, as determined by BrdU labeling, and CFSE and propidium iodide PI labeling. Based on cytological criteria, the proliferating cells were mostly spermatogonia and possibly also preleptotene spermatocytes. Differentiation of spermatocytes into spermatids or spermatozoa was also observed, mainly among the suspended cells. These results suggest that the organ and primary culture systems are suitable systems for studying the effects of substances that interfere with spermatogenesis in the medaka, a model vertebrate. The organ and primary culture systems were used to analyze the effects of a synthetic estrogen, EE2, on cell proliferation in medaka testis. Both organ and primary culture were suitable for this purpose consistently small concentrations (0.01 and 1 nM) of EE2 stimulated cell proliferation slightly, while higher concentrations (100 nM) had an inhibitory effect. To investigate the effect of phytoestrogens on cell proliferation in spermatogenesis, selected flavonoids [genistein (1, 10, 100 µg/ml), quercetin (0.01, 1, 100 µM), and 8-prenylnarigenin (0.001, 0.1, 1, 10 µM)] were added to medaka testis primary cultures. Genistein and quercetin inhibited cell proliferation in the cultures while 8-prenylnarigenin had no effect. In a second series of experiments the addition of genistein (10 µg/ml) to primary cultures significantly inhibited both cell proliferation and cell differentiation as determined by flow cytometry using CFSE/PI labeling. Durchflusszytometrie EE2 Genistein Medaka Spermatogenese Spermatogonien Zelldifferenzierung Zellproliferation EE2 cell proliferation flow cytometry genistein primary culture spermatogenesis spermatogonia ddc:32 rvk:WX 5500 Diole Durchflusscytometrie Endokrin wirksamer Stoff Genistein Japankärpfling Proliferation Spermatogenese Spermatogonium Zelldifferenzierung
2	Organ and primary culture of medaka (Oryzias latipes) testis: Test systems for the analysis of cell proliferation and differentiation Song, Miyeoun 18 July 2003 (has links) In cultured medaka testis fragments, cells remained viable for the entire culture period (17h), and spermatids that developed from spermatocytes were viable and motile. Primary cultures were characterized over a period of two days with respect to cell viability and the distribution of adherent and suspended cells. These two cell populations were maintained in dynamic equilibrium in vitro for several days. Proliferating cells were predominant among clusters of suspended cells, as determined by BrdU labeling, and CFSE and propidium iodide PI labeling. Based on cytological criteria, the proliferating cells were mostly spermatogonia and possibly also preleptotene spermatocytes. Differentiation of spermatocytes into spermatids or spermatozoa was also observed, mainly among the suspended cells. These results suggest that the organ and primary culture systems are suitable systems for studying the effects of substances that interfere with spermatogenesis in the medaka, a model vertebrate. The organ and primary culture systems were used to analyze the effects of a synthetic estrogen, EE2, on cell proliferation in medaka testis. Both organ and primary culture were suitable for this purpose consistently small concentrations (0.01 and 1 nM) of EE2 stimulated cell proliferation slightly, while higher concentrations (100 nM) had an inhibitory effect. To investigate the effect of phytoestrogens on cell proliferation in spermatogenesis, selected flavonoids [genistein (1, 10, 100 µg/ml), quercetin (0.01, 1, 100 µM), and 8-prenylnarigenin (0.001, 0.1, 1, 10 µM)] were added to medaka testis primary cultures. Genistein and quercetin inhibited cell proliferation in the cultures while 8-prenylnarigenin had no effect. In a second series of experiments the addition of genistein (10 µg/ml) to primary cultures significantly inhibited both cell proliferation and cell differentiation as determined by flow cytometry using CFSE/PI labeling. info:eu-repo/classification/ddc/32 ddc:32

Search results

Organ and primary culture of medaka (Oryzias latipes) testis: Test systems for the analysis of cell proliferation and differentiation / Organ und Primärzellkultur von Medaka Testis: Test Systeme zur Untersuchung des Zellproliferation und Zelldifferenzierung

Organ and primary culture of medaka (Oryzias latipes) testis: Test systems for the analysis of cell proliferation and differentiation