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Predictive value of normal sperm morphology in intrauterine insemination (IUI) : a structured literature reviewVan Waart, J. (Johannes) 12 1900 (has links)
Thesis (MMed)--Stellenbosch University, 2001. / ENGLISH ABSTRACT: The aim of the study was to conduct a structured review of the literature published on the
use of normal sperm morphology, as an indicator of male fertility potential in intrauterine
insemination (M) programs. Published literature in which normal sperm morphology
was used to predict pregnancy outcome in lUI during the period 1984 - 1998 was
reviewed.
Four hundred and twenty one articles were identified. Eighteen provided data that could
be tabulated and analyzed. Eight of the analyzed studies provided sufficient data for
statistical analysis. Six studies used the Tygerberg strict criteria and two the WHO
guidelines (1987, 1992). A meta-analysis of the six studies in the strict morphology
group yielded a risk difference (RD) between the pregnancy rates achieved in the patients
below and above the 4% strict criteria threshold of -0.07 (95% CI: -0.11 to -0.03;
p< 0.001). WHO criteria group (1987,1992) had insufficient data to be analysed.
Meta-analysis showed a significant improvement in pregnancy rate above 4% threshold
for strict criteria. Accurate evaluation of normal sperm morphology results should be an
integral part of evaluating the male factor. / AFRIKAANSE OPSOMMING: Die doel van die studie was om 'n gestruktureerde literatuuroorsig van die gepubliseerde
data oor normale sperm morfologie uit te voer om vas te stelof dit enige waarde het as
voorspeller van manlike fertiliteitspotensiaal in intra uteriene inseminasie (lUI)
programme. Gepubliseerde literatuur waar normale sperm morfologie gebruik IS om
swangerskapsuitkoms te voorspel met IUI in die tydperk 1984 - 1998 is nagegaan.
Vierhonderd een en twintig artikels is geïdentifiseer. Agtien het genoeg data gehad om te
kan tabuleer en analiseer. Agt van die geanaliseerde studies het voldoende data gehad vir
statistiese analise. Ses studies het die Tygerberg streng kriteria gebruik en twee die WGO
(1987, 1992) riglyne. 'n Meta-analise van die ses studies in die streng kriteria groep het
'n risiko verskil tussen swangerskapstempo in pasiënte onder en bo die 4% streng kriteria
afsnypunt, van -0,07 (95% betroubaarheidsindeks: -0.11 tot -0.03; p<O.OOl) getoon.
Die WGO kriteria (1987,1992) groep het onvoldoende data gehad om te kan analiseer.
Meta-analise het 'n bekenisvolle verbetering in swangerskapuitkoms bo die 4% afsnypunt
getoon vir die streng kriteria. Akkurate evaluasie van normale sperm morfologie
resultate behoort 'n integrale deel te wees van die proses om die manlike faktor in
infertilteitsbehandeling volledig te evalueer.
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Studies on the effect of compatible solutes, epididymal compounds, and antioxidants on the post-thaw motility and fertility of pellet frozen ram spermatozoa / by Luis Gabriel Sanchez Partida.Sanchez-Partida, Luis Gabriel January 1995 (has links)
Includes bibliographical references (leaves 232-257). / xv, 257 leaves : ill. ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Aims to determine if the compatible solutes (proline, glycine betaine, and trehalose), the epididymal compounds (taurine, hypotaurine and inositol) or the antioxidants (carnosine and ascorbic acid) in tris-citrate based diluents could improve the post-thaw survival and/or fertility of ram spermatazoa. / Thesis (Ph.D.)--University of Adelaide, Dept. of Animal Science, 1996?
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Investigating the effects of nicotine on the male reproductive systemMaartens, Pieter Johann 12 1900 (has links)
Thesis (MScMedSc)-- Stellenbosch University, 2013. / ENGLISH ABSTRACT: Much has been documented about the detrimental effects of adverse lifestyle factor
exposure on the body. Exposure to factors, such as cigarette smoke, have proved to not
only be a burden on global health and economy, but have also led to growing concerns
about effects on systemic functions such as reproduction. The aim of the present study was
to determine the effects of in utero and in vitro nicotine exposure on spermatozoal function
and the antioxidant enzyme activity and lipid peroxidation (LPO) status of the male
reproductive system. A better understanding of this process is necessary to combat the
respective burdens of smoking and male infertility and for the prospective development of
treatment strategies.
Two experimental models were employed: Wistar rats were exposed to nicotine in utero
while human and rat spermatozoa were exposed to nicotine in vitro. In utero studies were
achieved by selecting healthy pregnant rats and treating them with 1 mg/kg-bodyweight/day
nicotine or 1 ml/kg-bodyweight/day 0.85% physiologic saline throughout gestation and
lactation. Male rat pups were selected and sacrificed at each of the following age groups
(n=6): 42 days, 84 days and 168 days old. The pups were only exposed to the
treatment/saline via placental uptake or lactation. Biochemical analyses of the tissue
comprised of measurement of LPO and antioxidant enzyme activity. Results indicated a
significant association of maternal nicotine exposure to decreased levels of primary
antioxidant enzymes in rat testes. Of particular note was the observation that the treatment
group, of which each of the respective antioxidant enzyme levels were significantly less than
the control group, was the oldest (d168) rat group.
In vitro studies were achieved by collecting sperm samples from healthy human donors
(n=12), healthy rats (n=6) and obese rats (n=6). Samples were washed and exposed to
different concentrations of high levels of nicotine (Control, 0.1mM, 1mM, 5mM, 10mM) in
vitro. Semen parameters such as motility, viability and acrosome reaction were monitored at
different time points (30min, 60min, 120min, 180min). Results revealed increasing in vitro nicotine concentrations were associated with decreased viability and acrosomal status of
human spermatozoa and decreased progressive motility and viability of rat spermatozoa.
Obesity was also associated with decreases in progressive motility and viability of rat
spermatozoa.
These results indicate that the acute in vitro exposure of spermatozoa to high levels of
nicotine could adversely affect semen quality and may be an additive factor to the
impediment of male fertility. In utero results reveal maternal nicotine exposure adversely
affects male fertility in later life and seems to elicit more detrimental effects on the
reproductive system than that of direct nicotine exposure to spermatozoa. Obesity also
inhibits parameters of male fertility and these effects are exacerbated by nicotine exposure.
The authors believe these adverse effects on the reproductive system to be related to an
increased activation of leukocytes, excess production in reactive oxygen species (ROS) and
consequent onset of oxidative stress (OS). Nevertheless this study agrees with other studies
that nicotine exposure may be an additive factor to the impediment of male fertility. / AFRIKAANSE OPSOMMING: Daar is reeds baie bekend oor die moontlik newe effekte vir die liggaam wat met ‘n
ongesonde lewenstyl gepaard gaan. Menslike blootstelling aan sulke faktore, soos sigaret
rook, is wêreldwyd ‘n las vir gesondheid en ekonomie en het gelei tot geweldige kommer
onder navorsers oor die moontlike komplikasies vir liggaamlike funksies soos voortplanting.
Die doel van die betrokke projek was om die effekte van in utero en in vivo nikotien
blootstelling op die antioksiderende ensiem aktiwiteit en lipied peroksidasie status van
reproduktiewe weefsel en die funksionele parameters van spermatozoa te bepaal. ‘n Beter
begrip van hierdie proses is noodsaaklik om die las van rook en vetsug teen te werk en vir
die moontlike ontwikkeling van behandelingsstrategieë.
Twee eksperimentele modelle is ontwerp: Wistar rotte is in utero blootgestel aan nikotien
terwyl mens- en rot- spermatosoë ook in vitro aan nikotien blootgestel is. Vir die in utero
studie is gesonde dragtige rotte gedurende swangerskap en laktasie met 1 mg/kgliggaamsgewig/
dag nikotien of 1 ml/kg-liggaamsgewig/dag 0.85% fisiologiese soutoplossing
behandel. Manlike welpies is gekies en geoffer op elk van die volgende ouderdomme (n=6):
42 dae, 84 dae en 168 dae. Die welpies is slegs aan nikotien blootgestel deur plasentale
opname en laktasie. Biochemiese analise van die testikulêre weefsel het ‘n beduidende
assosiasie getoon tussen maternale nikotien blootstelling en verminderde vlakke van die
primêre antioksiderende ensieme. Die 168 dag oue groep het ‘n merkbare vermindering
getoon tussen kontrole en nikotien weefsel vir elk van die antioksiderende ensieme.
Vir die in vitro studie is sperm monsters verkry vanaf gesonde mans (n=12), gesonde rotte
(n=6) en vet rotte (n=6). Monsters is gewas en in vitro blootgestel aan verskeie hoë vlakke
van nikotien (kontrole, 0.1mM, 1mM, 5mM, 10mM). Seminale parameters soos motiliteit,
lewensvatbaarheid en akrosoom status is by verskei tydpunte gemeet (30min, 60min,
120min, 180min). Dit blyk dat verhoging in in vitro nikotien konsentrasies verband hou met
verlaagde lewensvatbaarheid en akrosoom status van menslike spermatosoë en verlaagde
progressiewe motilteit en lewensvatbaarheid van rot spermatosoë. Vetsug is ook geassosieer met verlagings in progressiewe beweeglikheid en lewensvatbaarheid van rot
spermatosoë.
In utero resultate openbaar dat maternale nikotien blootstelling manlike vrugbaarheid nadelig
beïnvloed in latere lewe en blyk dat dit meer van ‘n nadelige uitwerking op die
voortplantingstelsel het as dié van direkte nikotien blootstelling aan spermatosoë. In vitro
blootstelling van spermatosoë aan hoë vlakke van nikotien, het wel ook semen kwaliteit
nadelig beïnvloed. Vetsug inhibeer ook manlike vrugbaarheids parameters en hierdie effek
word vererger deur nikotien blootstelling.
Die outeure glo dat hierdie nadelige uitwerking op die voortplantingstelsel verband hou met
'n verhoogde aktivering van leukosiete, oortollige produksie van reaktiewe suurstof spesies
en die gevolglike aanvang van oksidatiewe stres bevorder. Hierdie studie stem wel ooreen
met ander studies wat nikotien blootstelling bestempel as ‘n bydraende faktor tot die
struikelblok van manlike onvrugbaarheid. / Harry Crossley Foundation (South Africa)
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The role of the cumulus oophorus complex during spermatozoa capacitational eventsRijsdijk, Michelle 12 1900 (has links)
Thesis (MScMedSc (Obstetrics and Gynaecology))--University of Stellenbosch, 2005. / Chapter 1 contains a review dealing with nuclear and morphological changes during spermatogenesis and spermatozoa transport with emphasis on the maturation of spermatozoa, capacitation, acrosome reaction and the interaction with the cumulus oophorus complex (COC). The oocyte and cumulus oophorus complex is also discussed particularly on the topic of maturity (oocyte and cumulus maturity). Also presented is a review of the fluorescent binding agents, namely Fluorescein Isothiocyanate labeled with Pisum sativum (FITC-PSA), Chlorotetracycline test (CTC) and Chromomycin A3 (CMA3). Chapter II describes all the materials and methods used during this study. Routine semen analysis is described with emphasis on normal spermatozoon morphology according to strict criteria. The evaluation of capacitation and acrosome reaction (AR) using the CTC and PSA-FITC staining methods as well as the evaluation of spermatozoon nuclear chromatin packaging using the CMA3 staining method is described. Chapter III represents the results recorded in this study. Compared with those spermatozoa cultured in medium alone, spermatozoa exposed to the cumulus mass were more likely to be capacitated and acrosome reacted, with a distinct increase in chromatin packaging quality. A general discussion of the results and future applications are discussed in Chapter IV. In short An in vitro model for spermatozoa penetration through the cumulus oophorus was established. The model can be applied to investigate the effect of the cumulus oophorus on sperm functions and to assist in the selection of functional sperm for intracytoplasmic sperm injection therapy. All relevant references are presented in Chapter V .
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Human gamete micromanipulation and intracytoplasmic sperm injection (ICI) : its impact on severe male infertilityWindt, Marie-Lena 12 1900 (has links)
Thesis (PhD)--Stellenbosch Uni versity, 2000. / ENGLISH ABSTRACT: Intracytoplasmic sperm injection (ICSI) introduced a revolutionary way of treatment for male
factor infertility. With the exception of some cases of non-obstructive azoospermia, all other
male factor infertility cases have the potential to be successfully treated with ICS!. The only
prerequisite seems to be the presence of a motile or viable immotile sperm cell for each oocyte.
In this study we report on our own experience with the development and implementation of the
ICSI method in the Reproductive Biology Unit at Tygerberg Hospita!. An analysis of 5 years of
ICSI experience showed that semen parameters, sperm morphology, motility and concentration
did not influence fertilization and pregnancy rates adversely. In most cases, patients who could
not be treated with in vitro fertilization (IVF) and gamete intrafallopian transfer (GIFT), due to poor
semen parameters or fertilization failure, were treated successfully with ICS!. Even a case of
globozoospermia was treated successfully with ICS!.
Testicular spermatozoa, fresh or frozen-thawed, also resulted in excellent fertilization and
pregnancy rates. Cryopreservation of testicular samples facilitated the management of the
infertile couple, aiding the coordination of the recovery of vital gametes from both partners and
also limiting the repetition of testicular biopsies. Incubation (maturation) of testicular spermatozoa
also induced an enhancement in pregnancy rates.
It can be concluded that ICSI proved to be a treatment method with success similar to that of in
vitro fertilization (IVF) and gamete intrafallopian transfer (GIFT), in spite of a severe male factor.
The study also indicated transfer route and embryo quality (viability) to be very important factors
in the success of ICS!. The tubal transfer route was shown to be a significant contributor to the
pregnancy success (compared to uterine transfer) as was the transfer of embryos that showed
early division to the 2-cell stage, 26 hours post injection. The transfer of early dividing embryos
into the fallopian tube resulted in a pregnancy rate of almost 40%, a result similar to that of GIFT
with a mild male factor.
The role of the oocyte in fertilization and pregnancy success was also revealed indirectly by the
introduction of ICS!. Visual observation of denuded oocytes was possible and many
morphological features, normal and abnormal, can be observed. Immature oocytes can also be
identified and it was shown that they could be successfully matured in vitro before injection.
In this study transmission electron microscopy (TEM) was used to study abnormalities in oocyte
morphology. The standard method was adapted and modified for single cell TEM. The
abnormalities observed included lysosomal and non-lysosomal degeneration (yellowish or darkly
coloured oocytes), degeneration and vacuole formation (vacuolated oocytes), large secondary
lysosomes filled with multiple small lipid droplets - lipofuscin body (refractile body) and a
fragmented oocyte. It was also possible to study at ultrastructural level, possible reasons for
fertilization failure in ICS!. Different stages of oocyte activation failure, cytoplasmic immaturity,
sperm cell extrusion, abnormal sperm cell decondensation, female spindle abnormalities and
technique related factors were observed. TEM was also successfully implemented to elucidate the reason for infertility in a patient with a
longstanding, unexplained history of infertility. TEM evaluation of two of the patient's unfertilized
oocytes revealed a spindle abnormality with contributing cytoskeletal anomalies at ultrastructural
level. The modified TEM technique offers a valuable tool to study this small, but important group
of patients with unexplained infertility. ThisTEM study opened up a new, valuable and interesting
avenue of research with both diagnostic and prognostic value for patients with unexplained
infertility.
ICSI is therefore a valuable method in the treatment of especially male factor infertility. It is the
most advanced fertilization technique developed in the last decade in this field. Not only can
almost all male factor patients be treated, but unexplained female infertility can also be exposed,
studied and hopefully in future also be treated with micromanipulation methods. / AFRIKAANSE OPSOMMING: Die ontwikkeling van die mikromanipulasie tegniek "Intracytoplasmic sperm injection" (ICSI)
het die behandeling van die manlike faktor in infertiliteit, revolusionêr verander. Met die
uitsondering van sommige gevalle van nie-obstruktiewe asoospermia, kan potensieel alle
ander manlike infertiliteits faktore suksesvol met ICSI behandel word. Die enigste voorvereiste
blyk "n bewegende of "n nie-bewegende, maar bewese lewende spermsel te wees.
In hierdie studie word verslag gedoen oor die ontwikkeling en toepassing van die ICSI metode in
die Eenhed vir Reproduktiewe Biologie by Tygerberg Hospitaal. 'n Analise van 5 jaar se resultate
na die implementering van die ICSI metode het gewys dat die semen parameters, sperm
morfologie, motiliteit en konsentrasie, nie "n effek op bevrugting- en swangerskapsyfers gehad
het nie. Pasiënte wat, as gevolg van ontoereikende semen parameters, nie met die klassieke
metodes, in vitro bevrugting (IVB) of gameet intrafallopiusbuis terugplasing (GIFT) behandel kon
word nie, kon suksesvol met ICSI behandel word. Daar was selfs "n geval van manlike infertiliteit
as gevolg van globosoospermie, wat suksesvol met ICS behandel is.
Die ICSI metode het dit ook moontlik gemaak om uitstekende bevrugting- en swangerskap
resultate met testikulêre spermatosoa .(vars en gevries) te bereik. Die bevriesing van
testisweefsel het ook bygedra tot beter hantering van sulke pasiënte. Herhaalde testisbiopsies
word uitgeskakel en die koórdinasie van die verkryging van die manlike en vroulike gamete, word
ook vergemaklik wanneer testisweefsel in gevriesde vorm beskikbaar is. Die studie het verder
getoon dat wanneer testikulêre weefsel geïnkubeer word (om spermatosoa te laat matureer), die
swangerskapsyfers verhoog was.
Dit is dus duidelik dat die ICSI metode net so suksesvol soos die IVB en GIFT metodes toegepas
kan word, selfs en veral in gevalle van erge manlike faktor infertiliteit.
Die studie het ook verder getoon dat die plek waar embrios teruggeplaas word, asook die
embriokwalitiet van teruggeplaasde embrios, belangrike bydraende faktore in die ICSI
swangerskapsukses was. Embrioterugplasing in die buis van fallopius en terugplasing van
embrios wat vroeë 2-sel deling, 26 uur na ICSI getoon het, is uitgewys as faktore wat ICSI
swangerskap betekenisvol verbeter het. Dit was moontlik om "n swangerskapsyfer van ongeveer
40%, sootgelyk aan die van GIFT sonder "n erge manlike faktor, te bereik met die terugplasing
van ten minste een vroeë deler embrio in die fallopiese buis.
Die ICSI tegniek het ook indirek bygedra tot nuwe insigte met betrekking tot die rol wat die
vroulike eiersel (oësief in ICSI bevrugting speel. Oósiete word gestroop van hulomringende selle
vir die ICSI proses en kan dan maklik vir hul normale en abnormale morfologiese eienskappe
evalueer word. Oësiete wat immatuur is kan ook so geïdentifiseer word en dit is moontlik om hulle
suksesvol te matureer voor mikro-inspuiting.
Transmissie-elektronmikroskopie (TEM) is in die studie gebruik om die ultrastruktuur van
onbevrugde en abnormale oësiete te bestudeer. Hiervoor is "n bestaande tegniek gemodifiseer vir die hantering van "n enkele sel, in hierdie geval die oosiet. Lisasomale en nie-lisosomale degenerasie (oósiete wat geelof donker van kleur voorkom), degeneratiewe tekens en vakuole
(oësiete met vakuole), groot sekondêre lisosome gevul met klein lipieddruppels ('refractile body')
en 'n gefragmenteerde oosiet was van die morfologies abnormale eienskappe wat ultrastruktureel
geïdentifiseer is. Moontlike faktore wat 'n rol kan speel in nie-bevrugting na ICSI kon ook op
ultrastrukturele vlak met die tegniek geïdentifiseer word. Hierdie faktore het die volgende
ingesluit: die onvermoë van verskillende stadiums van oosiet aktivering, sitoplasmatiese
immaturiteit, uitwerping van die spermsel na die periviteliene spasie, abnormale spermsel
dekondensasie, vroulike spoelvormings abnormaliteite en tegniekgekoppelde faktore.
Die TEM tegniek is ook suksesvol aangewend om die infertiliteitsprobleem van 'n pasiënt wat vir
etlike jare aan onverklaarbare infertiliteit gely het, te identifiseer. TEM het op die ultrastrukturele
vlak gewys dat daar 'n spoel abnormaliteit in twee van haar onbevrugde oëslete was. TEM kan
dus baie vrugbaar gebruik word in hierdie groep pasiënte om onverklaarbare infertiliteit, wat
andersins ongeïdentifiseerd sou bly, te verklaar.
Die ICSI metode is die mees revolusionêre tegniek wat die afgelope dekade vir die behandeling
van veral manlike infertiliteit ontwikkel en baie suksesvol toegepas is. Die metode ook kan 'n
bydraende rol speel in die hantering van onverklaarbare infertiliteit veral ten opsigte van die
vroulike gameet. In die toekoms is dit moontlik dat selfs hierdie probleem met nuwe
mikromanipulasietegnieke opgelos sal kan word.
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Investigations on the in vitro effects of aqueous Eurycoma longifolia Jack extract on male reproductive functionsErasmus, Nicolete January 2012 (has links)
<p>Eurycoma longifolia (Tongkat Ali / TA) is a Malaysian shrub used to treat various illnesses including male infertility. Considering that TA is also used to improve male fertility and no report  / regarding its safety has been published, this study investigated the effects of a patented, aqueous TA extract on various sperm and testicular functions. Materials and Methods This study  / encompasses two parts (part 1: on spermatozoa / part 2: on TM3-Leydig and TM4-Sertoli cells). Part 1: Semen samples of 27 patients and 13 fertile donors were divided into two groups,  / washed and swim-up prepared spermatozoa, and incubated with different concentrations of TA (1, 10, 20, 100, 2000 &mu / g/ml) for 1 hour at 37° / C. A sample without addition of TA served as control. After incubation with TA,  / the following parameters were evaluated: viability (Eosin-Nigrosin test), total and progressive motility (CASA), acrosome reaction (triple stain technique), sperm production of reactive oxygen  / species (ROS / dihydroethidium test / DHE), sperm DNA fragmentation (TUNEL assay) and mitochondrial membrane potential (&Delta / &psi / m) (Depsipher kit). Part 2: TM3-Leydig and TM4-Sertoli cells  / incubated with different concentrations of TA (0.4, 0.8, 1.6, 3.125, 6.25, 12.5, 25, 50 &mu / g/ml) and control (without extract) for 48 and 96 hours. After incubation with TA, the following parameters were  / evaluated: viability (XTT), cell proliferation (protein assay), testosterone (testosterone ELISA test) and pyruvate (pyruvate assay). Results Part 1: For washed spermatozoa, significant  / dose-dependent trends were found  / for viability, total motility, acrosome reaction and sperm ROS production. However, these trends were only significant if the highest concentrations were included in the calculation. In the swim-up spermatozoa, ROS production of spermatozoa showed a biphasic relationship with its lowest percentage at 10 &mu / g/ml, yet, no significance could be  / observed (P=0.9505). No influence of TA could be observed for sperm DNA fragmentation nor &Delta / &psi / m.</p>
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Investigations on the in vitro effects of aqueous Eurycoma longifolia Jack extract on male reproductive functionsErasmus, Nicolete January 2012 (has links)
<p>Eurycoma longifolia (Tongkat Ali / TA) is a Malaysian shrub used to treat various illnesses including male infertility. Considering that TA is also used to improve male fertility and no report  / regarding its safety has been published, this study investigated the effects of a patented, aqueous TA extract on various sperm and testicular functions. Materials and Methods This study  / encompasses two parts (part 1: on spermatozoa / part 2: on TM3-Leydig and TM4-Sertoli cells). Part 1: Semen samples of 27 patients and 13 fertile donors were divided into two groups,  / washed and swim-up prepared spermatozoa, and incubated with different concentrations of TA (1, 10, 20, 100, 2000 &mu / g/ml) for 1 hour at 37° / C. A sample without addition of TA served as control. After incubation with TA,  / the following parameters were evaluated: viability (Eosin-Nigrosin test), total and progressive motility (CASA), acrosome reaction (triple stain technique), sperm production of reactive oxygen  / species (ROS / dihydroethidium test / DHE), sperm DNA fragmentation (TUNEL assay) and mitochondrial membrane potential (&Delta / &psi / m) (Depsipher kit). Part 2: TM3-Leydig and TM4-Sertoli cells  / incubated with different concentrations of TA (0.4, 0.8, 1.6, 3.125, 6.25, 12.5, 25, 50 &mu / g/ml) and control (without extract) for 48 and 96 hours. After incubation with TA, the following parameters were  / evaluated: viability (XTT), cell proliferation (protein assay), testosterone (testosterone ELISA test) and pyruvate (pyruvate assay). Results Part 1: For washed spermatozoa, significant  / dose-dependent trends were found  / for viability, total motility, acrosome reaction and sperm ROS production. However, these trends were only significant if the highest concentrations were included in the calculation. In the swim-up spermatozoa, ROS production of spermatozoa showed a biphasic relationship with its lowest percentage at 10 &mu / g/ml, yet, no significance could be  / observed (P=0.9505). No influence of TA could be observed for sperm DNA fragmentation nor &Delta / &psi / m.</p>
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Odběr spermatu pomocí katetru a jeho využití při výtěru štiky obecné (Esox lucius L.)PLAŇANSKÝ, Tomáš January 2018 (has links)
The aim of this diploma thesis is to compare quality of northern pike sperm collected by different methods. First method is collection of stripped sperm by abdominal massage of the belly. Second method is collection of stripped sperm with special catheter to eliminate sperm contamination by urine. The last method is collection of testicular sperm. Differently collected sperm was evaluated and compared its quality. The main observed parameters were sperm volume, spermatozoa concentration, spermatozoa motility and velocity and osmolality of seminal fluid. Sperm samples were used for eggs fertilization. In fertilized eggs, the fertility of eggs and larvae hatching rate were observed.
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