No estado do Espírito Santo (ES), uma das principais áreas produtoras de mamão do país, a eliminação sistemática de plantas doentes tem sido aplicada desde a década de 1980 para o controle do mosaico do mamoeiro (Papaya ringspot virus - type P; PRSV-P). O uso permanente dessa prática nos últimos 25 anos levou a uma aparente seleção e predominância de isolados fracos do vírus. Os objetivos deste trabalho foram: investigar a prevalência desses isolados fracos, bem como a estabilidade e o efeito protetor contra isolados severos do vírus; estudar a infecção natural de abobrinha de moita (Cucurbita pepo cv. Caserta) e abóbora moranga (C. maxima cv. Exposição) com o PRSV-P quando plantadas ao lado de mamoeiros infectados e caracterizar um isolado do Alfalfa mosaic virus (AMV) em infecção natural em mamoeiro. A detecção de possíveis isolados fracos do vírus foi realizada por PTAELISA, microscopia eletrônica e RT-PCR. Todos os isolados também foram inoculados mecanicamente em mamoeiro cv. Golden para avaliação de sintomas. Sequências de nucleotídeos e de aminoácidos deduzidos do gene da proteína capsidial de alguns isolados fracos mostraram identidades superiores a 89% e 90%, respectivamente, com isolados do PRSV-P. De 119 amostras de mamoeiros analisadas, 86 estavam infectadas com o PRSV-P, mas somente 75 induziram sintomas fracos em mamoeiros. Quatro isolados fracos foram selecionados ao acaso para estudos de estabilidade, e de proteção em casa de vegetação. Apenas dois isolados fracos induziram sintomas estáveis em mamoeiros até a oitava transferência. A proteção total só foi obtida com plantas premunizadas com dois isolados fracos e desafiados com o isolado PRSV-PES. Plantas de mamoeiros cv. Golden premunizadas com vários isolados fracos do PRSV-P foram expostas em condições de campo na ESALQ/USP, Piracicaba, SP, em dois experimentos independentes. Poucas plantas permaneceram com sintomas fracos de mosaico até o final dos experimentos. Uma terceira exposição foi realizada em Linhares, ES, com mamoeiros cvs. Sunrise Solo e Golden premunizados com oito isolados fracos, coletados nos experimentos em campo na ESALQ/USP. Apenas uma planta premunizada com um isolado fraco permaneceu com sintomas leves da doença até a última avaliação. Tentativas de detectaçao de infecções naturais de cucurbitáceas com o PRSV-P foram realizados em dois plantios de abobrinha de moita e dois de abóbora moranga, na ESALQ/USP, Piracicaba, SP. A detecção do vírus foi feita por meio da inoculação de extratos foliares das cucurbitáceas em mamoeiros cv. Golden. Os mamoerios foram avaliados por meio de sintomas, PTA-ELISA e RT-PCR. Nenhuma planta de mamoeiro inoculada com extratos foliares das duas cucurbitáceas exibiu sintomas de mosaico, embora o gene ci, mas não o cp, tenha sido detectado em uma amostra de folhas de mamoeiro, indicando que ao menos uma planta de abobrinha de moita estava infectada. Finalmente, no decorrer dos ensaios de campo na ESALQ/USP, constatou-se uma planta de mamoeiro apresentando sintomas severos de mosaico amarelo, deformação foliar e necrose sistêmica, diferente daqueles induzidos pelo PRSV-P. Análises biológicas, sorológica e moleculares confirmaram tratar-se do AMV. Este é o primeiro relato de infecção natural de mamoeiro com esse vírus. / Papaya ringspot virus type P (PRSV-P) causes the major disease in Brazilian papaya orchards that result in significant yield losses. In Espírito Santo state systematic rouging of infected plants has been applied since early 1980s for the control of this disease. Its permanent use over the last 25 years has lead to an apparent selection and predominance of mild strains throughout papaya orchards. The objectives of this work were to investigate the prevalence of mild isolates, as well the stability and protective effect against severe isolates of the virus; The aim of this work was to study the natural infecction of zucchini squash (Cucurbita pepo cv. Caserta) and pumpkin (C. maxima cv. Exposição) grown near to papaya trees infected with PRSV-P and characterize an isolate of Alfalfa mosaic virus (AMV) in natural infection in papaya. The detection of possible mild isolates of the virus was performed by PTA-ELISA, electron microscopy and RT-PCR. All isolates were inoculated mechanically in papaya cv. Golden for symptoms evaluation. Nucleotides and deduced amino acids sequences of the coat protein gene of some mild isolates showed identities above 89% and 90%, respectively, with isolates of PRSV-P. Of 119 samples from papaya plants analyzed, 86 were infected with PRSV-P and 75 induced mild symptoms on papaya. Four mild isolates were randomly selected for stability and protection studies under greenhouse. Only two isolates induced mild symptoms on papaya and remained stables until the eighth transference. Full protection was obtained with preimmunized plants with two mild isolates and challenged with the isolate PRSV-P-ES. Plants of papaya cv. Golden preimmunized with several mild isolates of PRSV-P were exposed under field conditions at ESALQ/USP, Piracicaba,SP, in two independent experiments. Few plants remained with mild mosaic symptoms at the end of the experiments. A third field exposition was held in Linhares,ES, with papaya cvs. Golden and Sunrise Solo preimmunized with eight mild isolates, collected in field experiments at ESALQ/USP. Only one plant preimmunized with a mild isolate remained with mild symptoms of the disease until the last evaluation. Attempts of detection of natural infections of cucurbits with PRSV-P were carried out in two plantations of zucchini squash and pumpkin at ESALQ/USP, Piracicaba,SP. The detection of the virus was made by inoculation of leaf extracts of cucurbits in papaya cv. Golden. The papaya plants were assessed by symptoms, PTAELISA and RT-PCR. None of papaya plants exhibited symptoms of mosaic, while the ci gene, but not the cp, was detected in a sample of leaves of papaya, indicating that at least one clump of zucchini squash plant was infected. Finally, during the field test at ESALQ/USP, a papaya plant was found showing severe symptoms severe yellow leaf mosaic, leaf distortion and systemic necrosis, different from those induced by PRSV-P. Biological, serological and molecular tests confirmed the infection with AMV. This is the first report of natural infection of papaya with this virus.

The goal of this thesis is to optimize current marketing mixes in sports facilities. As such, this thesis will suggest appropriate optimization strategies for these mixes and recommend methods for improving marketing communication practices and service portfolios, in order that facilities may heighten their competitiveness, increase facility attendance, and enhance client satisfaction. The theoretical section of this thesis analyses marketing mixes, services, sports product, CRM, CEM, and facility environment. The practical component first presents the facility itself: a squash centre, where the analysis was done. An analysis and assessment of marketing activities of the squash centre was carried out afterwards. Quantitative forms of research, especially interviews, were used. Informal conversations were also held with the management of the squash centre. The thesis results in some recommendations for the squash centre which are applicable for sports facilities in general.

This master thesis deals with the design of a new sports center. The capacity of the sports center is 90 visitors and 12 employees. The building is designed as a two-storey, for disabled people and constructed by sandstone brick block with a flat roof. On the first floor is a main and staff entrance, a café, squash, 3 saunas (men, women, mixed), 2 rehabilitation rooms and staff room. On the second floor is a gym, HEAT, spinning, a mirror and gym hall, gallery and staff room. Part of the proposed building is a car park with a capacity of 70 parking spots (4 parking spots for disabled people) and 1 bus stop. Car park for staff has a 16 parking spot.

This diploma thesis deals with designing of a leisure sport centre in Partizánske in Slovak Republic. It is a detached object on a flat terrain. The object of the leisure sport center has two floors, or specifically the sport center stretches through two floors. In the building there is a cafe with bowling, three badminton courts, five squash courts and commercial area intended for massages. The object is designed from constructive system from lightweight concrete block. The second part is constructed from precast concrete frame with infill wall from lightweight concrete block. The ceiling in one part of the object is designed to be assembled of prestressed precast floor slab and in second part of trapezoidal sheet. The roof is made like flat green roof with extensive grassing. The work includes project documentation for the construction.

This Master´s Thesis deals with design and execution of project documentation for building of sport and wellness center. The site is situated in the outskirts of Vsetín in Zlín region. The structural system of the building is walled with porotherm blocks. Ceilings are made up of ceiling panels spiroll. Roof is mainly flat, pitched roof is only designed above squash courts. The buildings’ shape is composed of three blocks which together form a functional complex. The sport center offers these kinds of activities: spinning bike rides, exercising, weight lifting, group training and squash. The recreation centre offers massages electro and lymfo massages, pedicure, manicure, sauna, rehabilitation and solarium. Part of the center is also cafe providing also snacks, sport counseling, nutrition counseling and office.

Object SPORT & RELAX CENTER is a new building made by reinforced concrete monolithical skeleton. Filling masonry is creating with porous concrete blocks YTONG. Thermal insulation is attended by blocks made from mineral wool. Facade is ventilated and made from fibre cemented blocks. Object has two floors and cellar. The foundations are reinforced concreting monolithical stretches and foots. The stairs are three-arm monolithical reinforced concrete. Object is roofed by flat one-housing roof, over restaurant part is intensive green roof and terrace with ceramic tile.Object is supposed to use for sports and relaxation needs for the public of Chotěbor’s city and vicinity. In first floor we can find two courts for squash, restaurant with the terrace, in second floor is fitness center and in 1S we can find wellness center (massages, relaxing baths, whirlpool, relaxing room, 3 kinds of sauna-finland, steam and steam solt sauna). On the east and the south part of the parcel will be providing parking lot for visitors and employees. On the west part of the parcel is enter for supply. On the north are situated 2 courts and a volleyball pitch. The rest of the parcel cover light grass.

The theme of master’s thesis is the newly built sports center in Rosice. This work aids to develop design documents for building construction. The building is partial basement, four-storey. The building is designed of systém Heluz with cast-in-place reinforced concrete floors. The roof construction is single-flat, over part of the building is composed of timber truss Gang-nail. In the building there is the bar with bowling alley, squash, small multipurpose sports hall, fitness room, table tennis and spinning. In the basement are designed three saunas. Tennis courts with playground are outside next to the building.

Diploma thesis „Sport centre“ is made as a project documentation. The designed building structure is detached on a plane terrain in Lipník nad Bečvou. It has two floors and a basement. This building construction is based on the footings. The load-bearing walls are bricked up by Porotherm blocks. The ceilings are made up of panels Spiroll and PZD. The roof construction is designed as a single-flat. In building is squash, dance hall, gym, spinning, massages, snack bar and bowling with two lanes. Car park is next to the building.

The subject of my thesis is the design of new sports center in Hlučín. The sports center has three squash courts, a gym, spinning, two exercise rooms, a sauna and a bowling alley with a bar. The building is a basement, has two floors and is covered by a single casing flat roof. The structural system of brick Porotherm. The proposal respects the sports center city plan Hlučín existing street and buildings. Further emphasizes the layout including wheelchair access, collateral design for the static, architectural, energy conservation, fire safety and safety in use of the object.

This is detached sports center. The building is situated in the town of Ji­kovice town in Brno â Country Distric. This sports centre has two floors and it is partially with a cellar. The building is based on the footings of concrete. It is built from the building system POROTHERM and PREFA with contact insulation system from company ISOVER and ventilated facade. The roof construction is single-flat, on part is designed vegetation roof. Area buildings is 792,25 m2.