Submitted by Renata Lopes (renatasil82@gmail.com) on 2016-02-12T14:32:02Z No. of bitstreams: 1 luisfelipemarquesderesende.pdf: 659358 bytes, checksum: e233497985b7b59bfcd9389824074d23 (MD5) / Approved for entry into archive by Adriana Oliveira (adriana.oliveira@ufjf.edu.br) on 2016-02-26T12:17:06Z (GMT) No. of bitstreams: 1 luisfelipemarquesderesende.pdf: 659358 bytes, checksum: e233497985b7b59bfcd9389824074d23 (MD5) / Made available in DSpace on 2016-02-26T12:17:06Z (GMT). No. of bitstreams: 1 luisfelipemarquesderesende.pdf: 659358 bytes, checksum: e233497985b7b59bfcd9389824074d23 (MD5) Previous issue date: 2014-08-26 / O objetivo deste estudo experimental foi avaliar a influência de diferentes bebidas (água destilada, café, chá, vinho tinto e whisky) na estabilidade de cor de quatro diferentes resinas compostas e a efetividade do polimento final para recuperação da coloração inicial. As resinas utilizadas foram Filtek Z250 (microhíbrida), Filtek Z350 (nanoparticulada), IPS Empress Direct (nanohíbrida) e Tetric N-Ceram (nanohíbrida). Foram confeccionados 200 corpos de prova (8 mm x 2 mm), sendo 50 para cada tipo de resina e polidos com disco Sof-Lex em motor elétrico. Um aparelho espectrofotômetro digital foi utilizado para verificação da coloração inicial, após essa avaliação as amostras foram separadas de acordo com a resina composta utilizada em grupos (n=10) e permaneceram imersas nas respectivas soluções durante 14 dias. Após esse período os corpos de prova foram lavados e secos para nova avaliação da cor. Posteriormente os corpos de prova eram polidos e lavados para nova medição no espectrofotômetro. O sistema de parâmetro da coloração utilizado foi o CIEL*a*b*. As alterações de coloração foram verificadas a partir de ΔE1 após 2 semanas e ΔE2 seguinte ao repolimento. Valores acima de 3,3 foram considerados inaceitáveis clinicamente. As diferenças de cor foram estatisticamente comparadas pelo ANOVA two-way e test de Tukey (p<0,05). Com os resultados foi possível observar que o vinho tinto apresentou a maior variação de ΔE seguido pelo café, chá, whisky e água destilada. A resina microhíbrida Filtek Z250 apresentou a melhor estabilidade de cor em todas as substâncias líquidas. O repolimento final não foi efetivo para que as resinas compostas retornassem a sua coloração inicial e nenhuma das resinas apresentou estabilidade de cor clinicamente aceitável em qualquer das bebidas empregas neste estudo. / The aim of this study was to evaluate the influence of different beverages (distilled water, coffee, tea, red wine and whisky) on color stability of four different composites and the effectiveness of the polish to recover the initial color. The resins used were Filtek Z250 (microhybrid), Filtek Z350 (nanoparticles), IPS Empress Direct (nanohybrid) and Tetric N-Ceram (nanohybrid). A total of 200 specimens (8 mm x 2 mm), 50 for each type of resin were prepared and polished with Sof-Lex disc and electric motor. A digital spectrophotometer device was used to verify the initial color, after this evaluation samples were separated in accordance with the composite resin in groups (n = 10) and remained immersed in the respective solutions for 14 days. After this period the specimens were washed and dried for other evaluation of color. Subsequently, the composites were polished and washed to new measurement in the spectrophotometer. The system parameter staining was used CIEL * a * b *. The color changes were observed from ΔE1 after two weeks and following the repolishing ΔE2, values ΔE≥3.3 are considered clinically unacceptable. Color differences were statistically compared using ANOVA two-way and Tukey test (p <0.05). With the results was observed that red wine had the highest variation of ΔE followed by coffee, tea, whisky and distilled water. The microhybrid Filtek Z250 showed better color stability in all beverages. The repolishing was not sufficient to composite resins return to its original color and neither resin was color stability clinically acceptable in any of the drinks incorporate in this study.

Não consta resumo na publicação. / Abstract not available.

In couples diagnosed with unexplained infertility, the woman has normal ovulatory cycles and no organic pathology, and the man has normal semen. The period during which the implantation of an embryo can occur is defined as the window of implantation (WOI). For infertile women, disturbances in the endometrium maturation are hypothesized to impair the uterine receptivity for embryo implantaition. However, there are still no methods found to predict endometrial receptivity. Hemoglobin related-proteins hypothesized to be involved in embryo implantation have been found in the endometrium in both fertile and infertile women. The aim of this study was to determine whether there were differences in the expression of hemoglobin-related proteins, hemoglobin-α, cytoglobin, fetal hemoglobin and haptoglobin in the endometrium of fertile and infertile women. Immunohistchemical staining was used. Haptoglobin showed negative expression in glands, luminal epithelium and strong expression in stroma for both gruops. The expression of the hemoglobin-related proteins, hemoglobin-α, cytoglobin, fetal hemoglobin intensity were stronger in fertile than infertile women. More studies are required to find other factors that may have an effect on fertility.

Background. Immunohistochemistry is a useful method in the differential diagnosis between pleural mesotheliomas and metastatic adenocarcinomas in the pleura. Cytokeratin 20 and 7 have been used successfully as markers in studies determining primary location of adenocarcinomas from metastases. The current study is a complementary research of archived paraffininbedded material of cases with cancer origin. This study contributes a bigger statistical material that may facilitate the search for unknown primary site of adenocarcinoma by identification of metastatic cells in the pleura. Methods. Cells from the pleura taken from fifteen patients with diagnosed cancer of different types and eleven patients with cancer of unknown origin, were stained with antibodies against the tumour markers: Ber EP 4, calretinin, cytokeratin 20 and 7, estrogen receptor α, thyroid transcription factor, prostate-specific antigen and Cdx2.The staining was conducted in an automated immunohistochemical system. The staining of each kind of antibody was confirmed by a control section staining. Results. All control staining ended perfect The whole panel of antibodies used on mammary cancer showed the same pattern for every antibody. Of the patients with cancer of unknown origin there were four that gave the same pattern, two men and two women. The women are deceased. To make a more careful evaluation more information and clinic background is needed. The number of samples is too small to draw any statistical conclusions. Comment. Although the control staining was perfect the negative result of CK20 in the cases of diagnosed colon cancer was unexpected. This staining should be performed again to confirm the result. In some cases the number of cells were to few for a certain evaluation. The slides and the results of this work will be archived for further research.

Northern pike or pike Esox lucius is total piscivorous fish species found in the brackish and freshwater ecosystems of the Northern hemisphere. It is a top predator and keystone species that causes cascading effects in these ecosystems. Otoliths have been used to estimate age and growth rates in most fishes. Cleithra have generally have been used in Pike due to opaque nature of the otoliths. The aim of this study was to develop a method for age and growth estimation of pike using sagittal otoliths and carry out age and growth rates estimation of four sub populations; Dunöbäcken, Kårehamn, Oknebäcken and Väderön that represent freshwater and brackish water spawning sites. Determination of growth rates differences between sub populations was done for the first 2 years of their life. The methods included burning of the otoliths, fixing the otoliths in the epoxy resin, polishing of otoliths and staining with Alizarin red, Silver nitrate solution, Tetracycline and alkaline Lugol's solution with observation under light, dissecting and epifluorescence microscopes. Four methods tested based on staining with Alizarin red, Silver nitrate, alkaline Lugol's solution and burning otoliths. Provided useful results and not the method with Tetracycline. Alkaline Lugol's solution staining method was chosen and further developed. There was a significant difference in the relative growth rates between the sub population from brackish water spawning site of Väderön and the sub population from freshwater spawning site of Kårehamn in the first year (One-way ANOVA:F value 3.18, p value 0.0356) and after Tukey post hoc test (p value 0.0362). No difference among sub populations from the freshwater spawning sites. There was no difference in growth rates between any other sub populations in the second year. The importance of the study is successful application of Lugol's solution staining method for age and relative growth estimation studies with otoliths in pike. / EcoChange

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / This study aimed to evaluate the optical and surface behaviors of composite resin, when submitted to brushing and immersion in different medium. There were fabricated 60 specimens (cps) of a microhybrid resin (Filtek Z250) and of a nanoparticulate resin (Filtek Z350XT), using a metalized matrix (8 mm diameter x 2 mm thickness). The cps were photo activated with a LED (800mW/cm2) for 40s, then, they were polished with sandpaper disks and felt disks with diamond paste, washed with water and dried with absorbent paper. After 24 hours of hydration in deionized water at 37ºC, they divided into six experimental groups (n=10) according to the storage medium and type of treatment (stored in deionized water or red wine: not brushed, brushed with water and brushed with toothpaste). The initial reading (after 24h of hydration in water) about the surface roughness (Ra) was done with the surface roughness gauge (Mitutoyo Surftest SJ-201P) and the CIE L* a* b* parameter with the spectrophotometer (SP60 EX-Rite). Only the top surface was exposed to the staining process, the other faces were isolated with white acrylic resin, after that, they were immersed into the storage medium. Three immersion cycles were done (5, 10 and 15 days) with 5 days of duration. After the immersion periods, the resin was removed, the cp was again evaluated according to the CIE L* a* b* parameters and the surface roughness. The top surface was submitted to 585 brushing cycles in each 5 days. Successive readings were done after 5th and 15th days of immersion and after the first and third brushings. The data were submitted to Variance Analysis and Tukey test. The red wine had more impact than the deionized water on the color change of composite resins. The simulated brushing did not totally remove the pigments and its accumulation was dependent on the material composition, being the nanoparticulate resin more susceptible to this action. The composite resin showed similar behavior in relation to the surface roughness, and the toothpaste brushing promoted more abrasion than the water brushing. / Este estudo se propôs avaliar o comportamento óptico e superficial de resinas compostas, quando submetidas à imersão em diferentes meios e à escovação. Foram confeccionados 60 corpos-de-prova (cps) de uma resina microhíbrida (Filtek Z250) e uma resina nanoparticulada (Filtek Z350XT), utilizando matriz metálica nas seguintes dimensões 8 mm diâmetro x 2 mm espessura. O cps foram fotoativados com um LED (800mW/cm2) por 40s, polidos com discos de lixa e disco de feltro com pasta diamantada, lavados com água e secos com papel absorvente. Após 24 horas de hidratação em água deionizada a 37ºC, foram divididos em seis grupos experimentais (n=10) de acordo com o meio de armazenamento e tipo de tratamento (armazenados em água deionizada ou vinho tinto suave: não escovado, escovado com água e escovado com dentifrício). A leitura inicial (após 24h de hidratação em água) da rugosidade superficial (Ra) foi realizada com o rugosímetro (Mitutoyo Surftest SJ-201P) e dos parâmetros CIE L* a* b* com espectrofotômetro (SP60 EX-Rite). Somente a superfície de topo foi exposta ao processo de manchamento, as demais faces foram isoladas com cera acrílica branca, após os cps foram imersos nos meios de armazenamento. Foram realizados 3 ciclos de imersão (5, 10 e 15 dias) com 5 dias de duração. Após os períodos de imersão, a cera foi removida, o cp foi novamente aferido quanto aos parâmetros CIE L* a* b* e rugosidade superficial. A superfície de topo foi submetida a 585 ciclos de escovação simulada a cada 5 dias. Sucessivas leituras foram realizadas após o 5º e 15° dia de imersão e após a primeira e terceira escovação. Os dados foram submetidos à Análise de Variância e teste de Tukey. O vinho tinto teve maior impacto do que a água deionizada sobre a alteração de cor das resinas compostas. A escovação simulada não realizou a remoção total de pigmentos e o manchamento foi dependente da composição do material, sendo a resina nanoparticulada mais susceptível. As resinas compostas apresentaram comportamentos semelhantes quanto à rugosidade de superfície e a escovação com dentifrício promoveu maior abrasão que a escovação com água.

>Magister Scientiae - MSc / Risk factors such as intrauterine and vaginal infection put pregnant women at risk for delivering preterm. Bacterial vaginosis (BV) is a polymicrobial clinical syndrome commonly diagnosed in women of reproductive age, with women of African descent with low socioeconomic status and previous preterm delivery at high risk. Although frequently isolated from healthy women, Gardnerella vaginalis has been most frequently associated with BV. There is limited data available on the prevalence of BV in Southern Africa; therefore, we embarked on a study to determine the prevalence of BV and G. vaginalis in predominantly black communities in the Western Cape, in order to establish the role of G. vaginalis in BV. Women attending various Maternity and Obstetrics units (MOU) in the Cape Peninsula with and without a history of pre-term delivery (PTD) were invited to participate in the study. Several factors were statistically associated with pregnancy history, including location of study population, parity, smoking and presence of clinical symptoms. The presence of G. vaginalis was determined by culture in 51.7% of the preterm delivery group (PTDG) and 44% of the full-term delivery group (FTDG) women. BV was detected in 31.13% of PTDG and 23.67% of FTDG by Gram stained analysis according to Nugent scoring criteria, with age and HIV status posing as risk factors. When comparing PTDG and FTDG for an association between the presence of G. vaginalis and BV, a stronger association was observed in the PTDG but it was not statistically significant. In both PTDG and FTDG, G. vaginalis was isolated significantly more often in women diagnosed with BV at 24.5% (p < 0.05). Antibiogram studies revealed both Metronidazole and Clindamycin resistant strains of G. vaginalis. G. vaginalis Biotype 7 is specifically associated with BV, while Biotype 2 appears to be associated with BV in women with a history of PTD. Accuracy of diagnostic tools were tested and it was determined that Nugent scoring is more sensitive in diagnosing BV (76.04%), but culture for G. vaginalis is more specific (83.21%). Although this study was limited in that we were unable to follow-up pregnancy outcomes, we were able to confirm the perceived role of G. vaginalis in BV. / South Africa

Direct Write (DW) defines an emerging group of technologies that allow the printing of electronic and other functional components out of vacuum, directly onto conformal surfaces. Both ink-jet and nozzle deposition technologies, which are seen to be complementary for the wide range of materials and processing required by industry, are employed in this project.Silver neodecanoate salt is sensitive to both light source, including ultra violet light (UV) and heat source, and is a good inkjet printing precursor when dissolved in xylene. We have studied the electrical properties of inkjet printed silver samples, derived from silver neodecanoate ink, and investigated the influence of UV treatment before thermal curing the silver samples. UV exposure at room temperature is believed to control the nucleation of silver particles. In addition we have studied the influence of thermal pre-treatment on the printed samples. This pre-treatment is thought to assist the neodecanoate precursor to form a uniform distribution of silver nanoparticles. The influence of UV exposure, thermal pre-treatment and the thermal curing conditions on subsequent track microstructure, and its influence on electrical resistivity is reported for glass substrates. Furthermore, a series of extruding experimental at different nozzle offset and pumping pressure settings are conducted based on the rheological property of silicone oil and silver paste to find the ideal condition for producing continuous tracks with good shape. These findings are used to simulate a computer model for further applications.

>Magister Scientiae - MSc / The aim of this study was to investigate the color stability of two provisional restorative materials used in crown and bridge cases upon exposure to different tea staining solutions and to evaluate the effectiveness of a polishing technique in removing the tea stains from the stained provisional restorative materials.

This diploma thesis deals with problems of dirtiness of surface layers of ETICS (External thermal insulation contact system). In the theoretical part, soil damping agents and processes occurring when adhering dirt are described. The practical part then evaluates the impact of the aspects described below on their dirtyness. At the same time, it monitors the differences in staining depending on the type of material and the manufacturer. A methodology for artificial staining with controllable and repeatable conditions was developed in a special simulator, and this methodology was also verified practically. Simultaneously, individual plasters were exposed to the natural environment, for comparison of laboratory and actual conditions.