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Mycoplasma gallisepticum (MG) is the organism that causes avian respiratory mycoplasmosis, leading to chronic respiratory disease in chickens and infectious sinusitis in turkeys (Stipkovits and Kempf, 1996; Levisohn and Kleven, 2000). It is also responsible for reductions in egg production and other economic losses in the poultry industry (Stipkovits and Kempf, 1996; Levisohn and Kleven, 2000). Commercially, layer chickens are vaccinated against MG in the pullet phase before lay. In this dissertation, the potential application of in ovo vaccination in layer embryos for the subsequent early protection of pullets against field-strain MG infections was investigated. The use of different sites of injection [air cell (AC) or amnion (AM)] and various dosages of live attenuated 6/85 (6/85MG) and ts-11 (ts-11MG) vaccine strains of MG delivered in ovo at 18 days of incubation were evaluated. Vaccine dosages of ts-11MG up to 7.25 x 105 CFU did not have negative effects on the hatch and posthatch results. However, only the 7.25 x 105 CFU dosage had a negative effect on overall BWG. The ts-11MG was not transmitted from vaccinated to sentinel birds at both the hatch and posthatch periods, and no subsequent MG DNA or serology responses were detected in response to the vaccine. Administration of the 6/85MG vaccine at dosages up to 1.73 x 103 CFU did not negatively affect hatchability or other posthatch variables. Antibody production against 6/85MG through d 42 posthatch with no associated hatch or posthatch mortalities were likewise observed. The high dose of 6/85MG (1.73 x 104 CFU) resulted in a greater than 15 % mortality at hatch and a greater than 40 % mortality during the first 2 wk posthatch. Trachea and bronchi lesion scores in the pullets were significantly increased when they were challenged at d 28 of age with RMG. However, birds that were in ovo-vaccinated with 6/85MGV exhibited no significant microscopic lesions due to the RMG challenge. The 1.66 x 103 CFU dosage of 6/85MGV is proposed to offer the best protection in layer pullets against field-strain MG infections.

This work analyzes the design and implementation of waveguides used to measure stress waves in solid mechanics via explicit finite element analysis and experimentation. Many areas of physics use waveguides where control of timing, location, or frequency of waves is imperative to functionality of a system. Split Hopkinson pressure bars (Kolsky bars) traditionally utilize straight waveguides during testing. Prior research produced the first bent wave guide for use in such an application, the coaxially embedded serpentine bar (CESB). Explicit finite element analysis (FEA) provides a modeling approach to understand the effects of pass and joint geometry and boundary conditions on the functionality of solid-mechanic waveguides like the CESB. FEA and experimentation also contrasts the functionality of welded joints and threaded joints. Novel waveguide designs that do not feature tubes are also detailed for use in dynamic mechanical testing and dynamic hardness indentation experiments. These designs feature acoustic lengths up to two orders of magnitude greater than their physical lengths.

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This research presents a simplified framework for the analysis of deployable trusses using the concentrated strain approach and uses it to provide key insights into the many design decisions to be made in the development of concentrated strain architectures. The framework uses Euler Column Theory to derive closed form solutions to estimate truss performance. The results are compared to a classical solution and shown to give similar results. A range of strut and hinge hierarchy choices are considered. Trusses composed of solid rods with rectangular flexures are shown to have significant axial and bending stiffness reductions due to the smaller cross-sectional areas and lower modulus of the flexures. Trusses composed of tubes are less sensitive to this because the flexure cross-sectional area does not dramatically change from that of the tube. A hinge material metric that properly weights flexure strain and modulus is presented to provide a basis for the comparison and selection of proper hinge materials. However, based on this metric, new materials with higher folding failure strain and higher modulus are needed. Finally, a concentrated strain deployable truss of solid rods was designed, manufactured, and tested. A truss performance index for column loading was used to compare this system with a distributed strain ATK-ABLE GR1 coilable boom system and an articulated ATK-ABLE SRTM boom system. It was demonstrated that the concentrated strain approach has the potential to achieve a higher linear compaction ratio and truss performance index for mass efficient deployable trusses than the distributed strain approach and the articulated approach. / Ph. D.

The mechanical behaviour of the three advanced high strength steel grades, Docol 600DP, Docol 1200M and HyTens 1000, has been experimentally investigated under various types of deformation, and material models have been developed, which account for the experimentally observed behaviour. Two extensive experimental programmes have been conducted in this work. In the first, the dual phase Docol 600DP steel and martensitic Docol 1200M steel were subjected to deformations both under linear and non-linear strain paths. Regular test specimens were made both from virgin materials, i.e. as received, and from materials pre-strained in various directions. The plastic strain hardening, as well as plastic anisotropy and its evolution during deformation of the two materials, were evaluated and modelled with a phenomenological model. In the second experimental program, the austenitic stainless HyTens 1000 steel was subjected to deformations under various proportional strain paths and strain rates. It was shown experimentally that the material is sensitive both to dynamic and static strain ageing. A phenomenological model accounting for these effects was developed, calibrated, implemented in a Finite Element software and, finally,validated. Both direct methods and inverse analyses were used in order to calibrate the parameters in the material models. The agreement between the  numerical and experimental results are in general very good. This thesis is divided into two main parts. The background, theoretical framework and mechanical experiments are presented in the rst part. In the second part, two papers are appended.

Protective clothing ensembles are worn by workers as a barrier to chemical and physical hazards, but can restrict heat loss and increase worker heat stress. The question of whether a respirator adds to heat stress or strain burden is a continuing concern among occupational health professionals. The purpose of this study was to determine if there are differences in heat stress or strain among the current Toxicological Agent Protective (TAP) ensemble and two ensemble variations used in demilitarization of chemical weapons. Four acclimatized adult males wore five ensembles in a balanced design while walking in a climatic chamber at a metabolic rate of about 170 W m-2. Heat stress (critical wet bulb globe temperature-WBGTcrit, evaporative resistance-Re,T,a, Clothing Adjustment Factor [CAF]) and heat strain (physiological strain index [PSI]) were compared against work clothes (WC) without respirator (a baseline ensemble); the current TAP apron over cloth coveralls with respirator (TAP+CA); the current TAP apron over cloth coveralls with respirator plus Tychem F® chemical barrier pants (TAP+CA+P); and Tychem F® Coveralls over cloth coveralls with respirator (VB+CA). A no-respirator comparison with the Tychem F coveralls (VB+CA-noR) was added to evaluate the contribution of a full-face negative pressure air-purifying respirator to heat stress. A progressive heat stress protocol was used to determine WBGTcrit, Re,T,a, CAF, and PSI. The results (WBGTcrit [°C-WBGT], Re,T,a [kPa m2 W-1], and PSI) were WC (35.5, 0.0112, 2.0), TAP (31.6, 0.0175, 1.8), TAP+P (27.7, 0.0240, 1.9), VB+CA (25.9, 0.0287, 1.8), and VB+CA-noR (26.2, 0.0293, 1.8). Mixed effects ANOVA was used to assess ensemble effects. Tukey's test was used to determine where significant differences occurred. WBGTcrit was the WBGT at the upper limit of thermal balance. Re,T,a increased while WBGTcrit progressively decreased going from WC to TAP+CA to TAP+CA+P to VB+CA. WBGTcrit was different between Work Clothes and TAP+CA and between WC and TAP+CA and the other ensembles. Re,T,a was different among all ensembles, except no differences in WBGTcrit and Re,T,a were observed between the presence and absence of a respirator with VB+CA. There were no differences among all ensembles for rectal temperature, heart rate, and PSI. Based on both WBGTcrit and Re,T,a, there were significant increases in heat stress going from WC to TAP+CA to TAP+CA+P to VB+CA. No differences in WBGTcrit, Re,T,a, and PSI were found for the presence or absence of a respirator, indicating no additional heat stress or strain burden. CAF is the WC WBGTcrit minus the ensemble WBGTcrit.. The recommended clothing adjustment factors (CAFs) are 0°C-WBGT for WC, 4 °C-WBGT for TAP+CA, 8 °C-WBGT for TAP+CA+P and 10 °C-WBGT for VB+CA. As vapor-barrier ensembles are sensitive to humidity, adding 2 °C-WBGT to VA+CA for a CAF of 12 °C-WBGT is recommended. This implicates the type of protective clothing ensemble worn will play a much bigger role in workplace heat stress effects and risk than the wear of a respirator.

This thesis analyzes and solves the problems of monitoring of constructions strains (small resistance changes) in a number of points of the structure. The essence of investigated systems is test of the strain gauges small resistance changes proportional to displacement or strain and reaching only thousandths of parts of percent. The main objects of research is strain detection circuits working under automatic multipoint balanced Wheatstone bridge and the current balancing methods. The introduction reveals the importance of investigated problem, formulates the aim and tasks, introduces publications and presentations of the author’s and the structure of the dissertation. The first chapter is a review of literature. It analyzes practical use of strain measurement methods, their advantages and disadvantages, opportunities to use these methods in automated systems. The chapter ends with formulated conclusions. The second section presents balanced methods of the strain monitoring. Analysis of balanced Wheatstone bridge device is made. New strain monitoring device by balancing currents are proposed and investigated. There are two possible ways: with two current sources and one current source. The third and fourth sections provide results of computer simulation and experimental models. The proposed devices of balancing currents has been modeled and tested in practice. Stability of the devices was tested and their work simulating in real conditions. Conclusions, references and the... [to full text] / Disertacijoje nagrinėjamos ir sprendžiamos konstrukcijų deformacijų (varžų mažų pokyčių) nustatymo daugelyje konstrukcijos taškų problemos. Tiriamų sistemų funkcionavimo esmė yra įtempių jutiklių varžos mažų pokyčių proporcingų poslinkiui ar deformacijai ir siekiančių tik tūkstantąsias procento dalis nustatymas. Pagrindiniai tyrimo objektai yra automatinių daugiataškių balansuojamo Vitstono tiltelio ir srovių balansavimo metodais veikiantys deformacijų nustatymo grandynai. Įvadiniame skyriuje nagrinėjamas problemos aktualumas, formuluojamas darbo tikslas ir uždaviniai, pristatomi autoriaus pranešimai ir publikacijos, pateikiama disertacijos struktūra. Pirmajame skyriuje pateikta literatūros apžvalga. Jame analizuojami praktikoje naudojami deformacijų nustatymo metodai, pateikiami jų privalumai ir trūkumai, galimybės naudoti šiuos metodus automatizuotose sistemose. Antrajame skyriuje pateikti konstrukcijų deformacijų balansuojami nustatymo būdai. Yra atlikta balansuojamo Vitstono tiltelio metodo analizė. Pateiktas naujas deformacijų stebėjimo balansuojant sroves metodas ir atlikta jo analizė. Jame išskiriami du galimi būdai: su dviem srovės šaltiniais ir su vienu srovės šaltiniu. Trečiajame ir ketvirtajame skyriuose pateikti kompiuterinio modeliavimo ir eksperimentinių tyrimų rezultatai. Buvo sumodeliuotas ir praktiškai patikrintas pasiūlyto srovės balansavimo metodo praktinis veikimas. Patikrintas įrenginių stabilumas ir jų darbas imituojant realias sąlygas. Disertacijos... [toliau žr. visą tekstą]