The development of a non-invasive method of visualizing gene expression in larger animals could revolutionize some aspects of gene research by opening up a wider variety of animal systems to explore; some of which may be better models of human systems. Presently, most gene expression studies employ Green Fluorescent Protein (GFP) transfected into the genome of the animal system. For larger animals, an x-ray equivalent of GFP would be desirable due to the high penetrating power of x-rays. A model gene modification system is to use the Sodium (Na) Iodide Symporter (NIS) which will cause the accumulation of iodine in cells which express the NIS. To non-invasively observe the dilute iodine accumulated by the cancer cells transfected with NIS in the head of small animals, such as a rat, two synchrotron-based imaging methods were studied: K-Edge Subtraction (KES) imaging and Fluorescence Subtraction Imaging (FSI).<p> KES needs wide monochromatic x-ray beams at two energies bracketing the K-edge of the contrast agent existing or injected in the tissues. The monochromatic beam in the synchrotron facility normally is prepared by a double crystal monochromator. The appearance of the azimuthal angle (tilt error) in the double crystal monochromator creates intensity variations across the imaging field. This misalignment was studied through another two synchrotron-based imaging methods, Diffraction Enhanced Imaging (DEI) and Multi-Image Radiography (MIR), which show this problem clearly in their processed images. The detailed analysis of the effect of the tilt error, how it affects the resulting images, and how to quantify such an error were presented in the thesis. A post processing method was implemented and the artifacts caused by the improper experimental settings were discussed.<p> With the wide monochromatic beam prepared by the double crystal monochromator, a sequence of KES experiments were done and the detection limit of KES was quantified at a projected amount of 17.5mM-cm iodine in a physical model of a rat head with a radiation dose of 2.65mGy. With the raster scan of the object relative to the monochromatic pencil beam, FSI was studied to obtain higher Signal to Noise Ratio (SNR) for local area and better detection limit compared to KES. The detection limit of FSI was measured as a projected amount of 2.5mM-cm iodine in the same physical rat head with a tolerable radiation dose of 24mGy. According to the comparison of these two imaging techniques with references to imaging time and area, radiation dose, spatial resolution, and SNR, it was concluded that these two imaging techniques can be used complementarily in imaging dilute contrast material. Due to the short imaging time and large imaging area, KES is used first to provide a global view of the object, locate the area of interest, do the preliminary diagnosis, and decide whether the further FSI is necessary. Due to its high SNR for the dilute sample, FSI can be used when the area of interest is known. The combination of these two imaging techniques will be very promising and powerful. To facilitate the comparison of KES and FSI, a quality factor was developed to evaluate the performance of the imaging system.<p> The measured detection limits in our experiments are far beyond the thyroidal iodine concentration of a rat (around 1mM). To further improve the performance of KES, a bent Laue crystal monochromator was designed to do the simultaneous iodine KES imaging which overcomes the artifacts in the iodine image caused by the temporal difference for a single set of images. The designed monochromator can provide two separated x-ray beams bracketing the K-edge of iodine at the same time with a very high spatial resolution which is only depends on the source size, a very high energy resolution which can almost compete with that of the double crystal monochromator, and an acceptable photon flux.

The development of a non-invasive method of visualizing gene expression in larger animals could revolutionize some aspects of gene research by opening up a wider variety of animal systems to explore; some of which may be better models of human systems. Presently, most gene expression studies employ Green Fluorescent Protein (GFP) transfected into the genome of the animal system. For larger animals, an x-ray equivalent of GFP would be desirable due to the high penetrating power of x-rays. A model gene modification system is to use the Sodium (Na) Iodide Symporter (NIS) which will cause the accumulation of iodine in cells which express the NIS. To non-invasively observe the dilute iodine accumulated by the cancer cells transfected with NIS in the head of small animals, such as a rat, two synchrotron-based imaging methods were studied: K-Edge Subtraction (KES) imaging and Fluorescence Subtraction Imaging (FSI).<p> KES needs wide monochromatic x-ray beams at two energies bracketing the K-edge of the contrast agent existing or injected in the tissues. The monochromatic beam in the synchrotron facility normally is prepared by a double crystal monochromator. The appearance of the azimuthal angle (tilt error) in the double crystal monochromator creates intensity variations across the imaging field. This misalignment was studied through another two synchrotron-based imaging methods, Diffraction Enhanced Imaging (DEI) and Multi-Image Radiography (MIR), which show this problem clearly in their processed images. The detailed analysis of the effect of the tilt error, how it affects the resulting images, and how to quantify such an error were presented in the thesis. A post processing method was implemented and the artifacts caused by the improper experimental settings were discussed.<p> With the wide monochromatic beam prepared by the double crystal monochromator, a sequence of KES experiments were done and the detection limit of KES was quantified at a projected amount of 17.5mM-cm iodine in a physical model of a rat head with a radiation dose of 2.65mGy. With the raster scan of the object relative to the monochromatic pencil beam, FSI was studied to obtain higher Signal to Noise Ratio (SNR) for local area and better detection limit compared to KES. The detection limit of FSI was measured as a projected amount of 2.5mM-cm iodine in the same physical rat head with a tolerable radiation dose of 24mGy. According to the comparison of these two imaging techniques with references to imaging time and area, radiation dose, spatial resolution, and SNR, it was concluded that these two imaging techniques can be used complementarily in imaging dilute contrast material. Due to the short imaging time and large imaging area, KES is used first to provide a global view of the object, locate the area of interest, do the preliminary diagnosis, and decide whether the further FSI is necessary. Due to its high SNR for the dilute sample, FSI can be used when the area of interest is known. The combination of these two imaging techniques will be very promising and powerful. To facilitate the comparison of KES and FSI, a quality factor was developed to evaluate the performance of the imaging system.<p> The measured detection limits in our experiments are far beyond the thyroidal iodine concentration of a rat (around 1mM). To further improve the performance of KES, a bent Laue crystal monochromator was designed to do the simultaneous iodine KES imaging which overcomes the artifacts in the iodine image caused by the temporal difference for a single set of images. The designed monochromator can provide two separated x-ray beams bracketing the K-edge of iodine at the same time with a very high spatial resolution which is only depends on the source size, a very high energy resolution which can almost compete with that of the double crystal monochromator, and an acceptable photon flux.

The objective of this thesis is to build a phrase recognition system under noisy environment that can be used in real-life. In this system, the noisy speech is first filtered by the enhanced spectral subtraction method to reduce the noise level. Then the MFCC with cepstral mean subtraction is applied to extract the speech features. Finally, hidden Markov model (HMM) is used in the last stage to build the probabilistic model for each phrase. A Mandarin microphone database of 514 company names that are in Taiwan¡¦s stock market is collected. A speaker independent noisy phrase recognition system is then implemented. This system has been tested under various noise environments and different noise strengths.

No description available.

This study was aimed at discovering the differences in how children responded to word problems involving an operation in which they had received formal instruction (subtraction) and word problems involving an operation in which they have not received formal instruction. Nineteen children were individually interviewed and were asked to attempt to solve 6 subtraction and 6 division word problems. Their solution strategies were recorded, and analysed with respect to whether or not they were appropriate, as to whether or not they modeled the structure of the problem, and as to how consistent the strategies were, within problem types. It was found that children tended to model division problems more often than subtraction problems, and also that the same types of errors were made on problems of both operations. It was also found that children were more likely to keep the strategies for the different interpretations separate for the operation in which they had not been instructed (division) than for the operation in which they had been instructed (subtraction). For division problems, the strategies used to solve one type of problem were seldom, if ever used to solve the other type of problem. For subtraction problems, children had more of a tendency to use the strategies for the various interpretations interchangeably. In addition, some differences in the way children deal with problems involving the solution of a basic fact, and those involving the subtraction of 2-digit numbers, were found. The 2-digit open addition problems were solved using modeling strategies about half as often as any other problem type. The same types of errors were made for both the basic fact and the 2-digit problems, but there were more counting errors and more inappropriate strategy errors for the 2-digit problems, and more incorrect operations for the basic fact problems. Finally, some differences were noted in the problem-solving behaviour of children who performed well on the basic fact tests and those who did not. The children in the low group made more counting errors, used more modeling strategies, and used fewer incorrect operations than children in the high group. These implications for instruction were stated: de-emphasize drill of the basic facts in the primary grades, delay the formal instruction of the operations until children have had a lot of exposure to word problem situations involving these concepts, use the problem situations to introduce the operations instead of the other way around, and leave comparison subtraction word problems until after the children are quite familiar with take away and open addition problems. / Education, Faculty of / Curriculum and Pedagogy (EDCP), Department of / Graduate

A research report submitted to the Faculty of Science, in partial fulfilment of the requirements for the degree of Master of Science, University of the Witwatersrand. Johannesburg, October 2016. / This research study makes an attempt at intervening in the Grade6learners’ use of models and strategies in solving addition and subtraction word problems based on Realistic Mathematics Education (RME) theory. RME theory advocates for the provision of understandable contexts that learners can relate with to support them in developing models and strategies, with specific reference to the empty number line model in assisting learners to develop an understanding of the structure of number and to work flexibly in solving addition and subtraction word problems. It is in understanding the models and strategies learners are using that we can begin to understand how the learners need to be supported in order to operate at the appropriate mathematics levels for their grade. Participants in this research study, forty boys and girls doing grade six, all with a weaker mathematical background, wrote the same tests in the form of pre test, post test and the delayed post test. After writing the pre test, the learners attended a series of six intervention lessons before writing the post tests. The intervention lessons encouraged learner engagement with word problems and the development of models as representations of problem situations and strategies which represent learner’s manipulation of models in an RME-advocated approach. Learner responses were analysed aiming at the identification of models and strategies they employed, as well as the correctness and success in solving the problems. The analysis found out that mainly there have been some improvements in the repeat sittings from predominantly using the column model with a lot of incorrect answers to using the empty number line with more correct answers. I would therefore encourage the maximum participation of teamwork amongst teachers for identifying and using efficient models and strategies in order to promote performance levels in mathematics through developing an understanding of the structure of number and working flexibly in solving addition and subtraction word problems. / LG2017

As complicações peri-implantares, posteriormente a colocação do implante em função, são frequentes e sua identificação precoce pode significar uma terapêutica adequada e consequentemente garantir o sucesso do terapêutica. A proposta neste trabalho foi avaliar longitudinalmente a densidade ótica do osso alveolar peri-implantar na região cervical, analisada, por meio da técnica de subtração radiográfica de um acervo de radiografias digitais adquiridas de pacientes previamente reabilitados com trinta e sete implantes osteointegrados imediatos na região anterior da maxila. Os controles radiográficos foram realizados em cinco momentos: na instalação das próteses, após 15,90,180 e 360 dias, e as radiografias intrabucais foram obtidas pela técnica do paralelismo de cilindro longo, com posicionadores do tipo Hanshin individualizados de forma a garantir a padronização das imagens. As imagens digitais obtidas foram alinhadas e equalizadas e por fim submetidas ao procedimento de subtração radiográfica. Os resultados indicaram que a metodologia de subtração radiográfica é válida para a avaliação da perda óssea peri-implantar, assim como demonstra a literatura. No entanto, a reprodução das angulações verticais e horizontais, apresentam-se como críticas na avaliação da densidade óptica da crista óssea e demandam grande habilidade do operador para a correta aplicação. Por meio dessa técnica, conseguimos avaliar de forma quantitativa e qualitativa as alterações na faces proximais nas imagens radiográficas do acervo analisado. A subtração radiográfica, por meio da ferramenta de histograma, permitiu quantificar de forma objetiva as diferenças encontradas nas análises. / The peri-implant complications, in addition to implant placement in function, are frequent and their early identification could mean a therapy appropriately and consequently guarantee the success of the treatment. The aim of this research was to longitudinally evaluate of the optical density of the peri-implant alveolar bone at the cervical region, analyzed by means of the radiographic subtraction technique, a collection of digital radiographs acquired from patients previously rehabilitated with thirty seven osseointegrated implants. The radiographic controls performed in five moments, at the installation of the prostheses, after 15,90,180 and 360 days, and the intraoral radiographs were obtained by parallelism technique, with Hanshin-type positioners individualized in order to guarantee the standardization of the images. The obtained digital images were aligned and equalized and finally submitted to radiographic subtraction procedure. The results indicated that the methodology of radiographic subtraction is valid for an evaluation of the peri-implant bone loss, as well as to demonstrated at the literature. However, the reproduction of the vertical and horizontal angles, are presented as critical in the evaluation of the optical density, and demand a great ability of the operator for a correct application. Through the technique we obtained a quantitative and qualitative evaluation in the area of proximal images in the radiographic images of the analyzed data. The histogram tool applied on the radiographic subtraction images allowed to quantify objectively as the differences found in the analyzes.

Recent advances in DNA sequencing technology have enabled entire genomes to be sequenced quickly and accurately, resulting in an exponential increase in the number of organisms whose genome sequences have been elucidated. While the genome sequence of a given organism represents an important starting point in understanding its physiology, the functions of the protein products of many genes are still unknown; as such, computational methods for studying protein function are becoming increasingly important. In addition, this wealth of genomic information has created an unprecedented opportunity to compare the protein content of different organisms; among other applications, this can enable us to improve taxonomic classifications, to develop more accurate diagnostic tests for identifying particular bacteria, and to better understand protein content relationships in both closely-related and distantly-related organisms.<p> This thesis describes the design, evaluation, and use of a program called Proteome Subtraction and Intersection (PSI) that uses an idea called genome subtraction for discovering protein-phenotype relationships and for characterizing differences in protein content in groups of organisms. PSI takes as input a set of proteomes, as well as a partitioning of that set into a subset of "included" proteomes and a subset of "excluded" proteomes. Using reciprocal BLAST hits, PSI finds orthologous relationships among all the proteins in the proteomes from the original set, and then finds groups of orthologous proteins containing at least one orthologue from each of the proteomes in the "included" subset, and none from any of the proteomes in the "excluded" subset.<p> PSI is first applied to finding protein-phenotype relationships. By identifying proteins that are present in all sequenced isolates of the genus <i>Lactobacillus</i>, but not in the related bacterium <i>Pediococcus pentosaceus</i>, proteins are discovered that are likely to be responsible for the difference in cell shape between the lactobacilli and <i>P. pentosaceus</i>. In addition, proteins are identified that may be responsible for resistance to the antibiotic gatifloxacin in some lactic acid bacteria.<p> This thesis also explores the use of PSI for comparing protein content in groups of organisms. Based on the idea of genome subtraction, a novel metric is proposed for comparing the difference in protein content between two organisms. This metric is then used to create a phylogenetic tree for a large set of bacteria, which to the author's knowledge represents the largest phylogenetic tree created to date using protein content. In addition, PSI is used to find the proteomic cohesiveness of isolates of several bacterial species in order to support or refute their current taxonomic classifications.<p> Overall, PSI is a versatile tool with many interesting applications, and should become more and more valuable as additional genomic information becomes available.

This thesis is related to the broad subject of automatic motion detection and analysis in videosurveillance image sequence. Besides, proposing the new unique solution, some of the previousalgorithms are evaluated, where some of the approaches are noticeably complementary sometimes.In real time surveillance, detecting and tracking multiple objects and monitoring their activities inboth outdoor and indoor environment are challenging task for the video surveillance system. Inpresence of a good number of real time problems limits scope for this work since the beginning. Theproblems are namely, illumination changes, moving background and shadow detection.An improved background subtraction method has been followed by foreground segmentation, dataevaluation, shadow detection in the scene and finally the motion detection method. The algorithm isapplied on to a number of practical problems to observe whether it leads us to the expected solution.Several experiments are done under different challenging problem environment. Test result showsthat under most of the problematic environment, the proposed algorithm shows the better qualityresult.

Recent advances in DNA sequencing technology have enabled entire genomes to be sequenced quickly and accurately, resulting in an exponential increase in the number of organisms whose genome sequences have been elucidated. While the genome sequence of a given organism represents an important starting point in understanding its physiology, the functions of the protein products of many genes are still unknown; as such, computational methods for studying protein function are becoming increasingly important. In addition, this wealth of genomic information has created an unprecedented opportunity to compare the protein content of different organisms; among other applications, this can enable us to improve taxonomic classifications, to develop more accurate diagnostic tests for identifying particular bacteria, and to better understand protein content relationships in both closely-related and distantly-related organisms.<p> This thesis describes the design, evaluation, and use of a program called Proteome Subtraction and Intersection (PSI) that uses an idea called genome subtraction for discovering protein-phenotype relationships and for characterizing differences in protein content in groups of organisms. PSI takes as input a set of proteomes, as well as a partitioning of that set into a subset of "included" proteomes and a subset of "excluded" proteomes. Using reciprocal BLAST hits, PSI finds orthologous relationships among all the proteins in the proteomes from the original set, and then finds groups of orthologous proteins containing at least one orthologue from each of the proteomes in the "included" subset, and none from any of the proteomes in the "excluded" subset.<p> PSI is first applied to finding protein-phenotype relationships. By identifying proteins that are present in all sequenced isolates of the genus <i>Lactobacillus</i>, but not in the related bacterium <i>Pediococcus pentosaceus</i>, proteins are discovered that are likely to be responsible for the difference in cell shape between the lactobacilli and <i>P. pentosaceus</i>. In addition, proteins are identified that may be responsible for resistance to the antibiotic gatifloxacin in some lactic acid bacteria.<p> This thesis also explores the use of PSI for comparing protein content in groups of organisms. Based on the idea of genome subtraction, a novel metric is proposed for comparing the difference in protein content between two organisms. This metric is then used to create a phylogenetic tree for a large set of bacteria, which to the author's knowledge represents the largest phylogenetic tree created to date using protein content. In addition, PSI is used to find the proteomic cohesiveness of isolates of several bacterial species in order to support or refute their current taxonomic classifications.<p> Overall, PSI is a versatile tool with many interesting applications, and should become more and more valuable as additional genomic information becomes available.