Made available in DSpace on 2015-05-14T12:59:32Z (GMT). No. of bitstreams: 1 arquivototal.pdf: 730230 bytes, checksum: c9ea66b757014e0b2d6d2b07b2de3af1 (MD5) Previous issue date: 2012-02-14 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / Acinetobacter species have gained importance in recent years due to their increased involvement in serious infections and antimicrobial resistance. Since multidrug resistant bacteria pose a challenge to the treatment of infections, it is necessary to find new substances with antimicrobial properties in combating these microorganisms. Thus, medicinal plants are studied, since they are used for treatment of various illnesses. Therefore, many studies on biological activity have been performed with essential oils obtained from medicinal plants, such as the essential oil from Citrus limon (EOCL), attempting to help overcome this problem. Thus the present study aimed to evaluate the effect of the essential oil of EOCL against multidrug resistant strains of Acinetobacter spp. Its chemical composition was determined by GC/MS and its antibacterial activity was assessed by determining their MIC and MBC by microplate bioassay, its Time kill too was determinated. Also was analyzed the effect of modulating action of conventional antibiotics by microplate bioassay. Among phytochemicals, Neral (29.4%) presented as the major essential component of EOCL. The oil caused growth inhibition in 16 (67%) of 24 strains tested, showing a MIC of 625 μg/ml and MBC of 1250 μg/mL. In a time kill study, the oil displayed a concentration-dependent antibacterial activity. Results of combining antibiotics and EOCL had shown us a synergistic and additive effect with EOCL /antibiotics combinations. The results of this study suggest that essential oil of C. limon may suppress the growth of Acinetobacter species and could be a source of metabolites with modified antibacterial activity. / Espécies do gênero Acinetobacter ganharam importância nos últimos anos devido ao seu envolvimento crescente em infecções graves e resistência antimicrobiana. Uma vez que bactérias resistentes a múltiplas drogas representam um desafio para o tratamento de infecções, é necessário encontrar novas substâncias com propriedades antimicrobianas no combate a estes microrganismos. Para tentar superar este panorama, várias pesquisas vêm sendo desenvolvidas em busca de novos produtos antibacterianos, dentre elas estão os estudos com os metabólitos de plantas, como o óleo essencial de Citrus limon (OECL). Assim, o presente estudo teve como objetivo avaliar o efeito do óleo essencial de Citrus limon contra cepas de Acinetobacter multidroga resistentes. Sua composição química foi determinada por CG/EM e sua atividade antibacteriana avaliada pela determinação de sua CIM e CBM pela técnica de microdiluição, sua cinética de morte microbiana também foi analisada. Verificou-se também seu efeito modulador da ação dos antibióticos convencionais por técnicas de microdiluição. Entre os fitoconstituintes, neral (29,4%) apresenta-se como o principal componente de OECL. O referido óleo inibiu o crescimento de 67% das 24 cepas ensaiadas, tendo sua CIM estabelecida em 625 μg/mL e a CBM em 1250 μg/mL Este óleo possui atividade antibacteriana dependente de sua concentração e do tempo de exposição no microrganismo e é um modulador da ação dos antibióticos convencionais. Os resultados deste estudo sugerem que o referido óleo pode suprimir o crescimento de espécies de Acinetobacter e poderia ser uma fonte de metabólitos com atividade modificadora antibacteriana.

Cement bonded particleboards contain organic base and as they are set outside, extreme weather condition, such as frost, chemical defrosting substances or sulphates, cause their degradation. The presence of the organic base in the matrix of the material could be the reason why there is higher probability of degrading when compared with materials containing an inorganic base. It could be partially eliminated by an appropriate surface modification of the slab, which main goal is to weaken the impact of an aggressive environment and to extend the lifespan of the board. On the other hand, the surface modifications based on a polymer base are prone to degrade because of other unfavourable conditions such as UV radiation when compared with the cement bonded particleboard. The classification of the surface modifications and the way of their degradation caused by the aggressive environment in real applications are presented in the theoretical part of the thesis. In the experimental part, appropriate methodical ways of testing the resistance of cement bonded particleboard’s coating system were chosen. Then, the testing and comparing of the physical, mechanical and aesthetic properties of different types of coatings were done. Moreover, a spectrophotometric analysis of the colour shade changes for the different aggressive environment were carried out. Furthermore, microstructures of chosen surface modifications were assessed by using an optical and an electron microscope.

This diploma thesis is aimed at assessing the existence of synergistic effects of the selected consolidated entities in the Czech Republic. Using specific financial metrics, the objective of this thesis is to identify the synergistic effects which occur among five consolidated entities operating in the area of property. Measurement and evaluation are performed pursuant to analysis of financial statements as well as qualitative data and are supposed to lead to assessing the existence of synergy in given sphere of national economy.

No description available.

This thesis is focused on the issue of achieving synergistic effect in a consolidating entity. Consolidation unit and proposed financial indicators for monitoring the synergistic effect of the parent company and the consolidated group are defined based on the theoretical findings. Based on the analysis of these indicators is evaluated whether the synergistic effect is achieved.

The thesis focuses on the methods of assessment of synergies established in a consolidated entity. The text includes a definition of a consolidated unit based on theoretical grounds and a draft set of indicators suitable for the analysis of the achievement of synergies. Synergies are monitored through a comparison between the consolidated unit and the mother company made in several selected areas. Results of the analysis of the draft indicators are assessed to determine whether synergies were achieved.

The diploma thesis deals with assessing the synergistic effects of the selected consolidated entity. The analytical part consists of a valuation of the current economical situation in the particular consolidated entity. The thesis determines appropriate indicators of evaluating the synergic effect. The results of these indicators demonstrate the existence of synergy.

The aim of this thesis is evaluation of synergy effects existence in a chosen consolidations subject. Joining of enterprises focuses on attaining certain advantage – synergy effect. This effect should be achieved in various fields. Financial indicators were chosen from theoretical presumptions and it will be finding, if there is a synergy effect in chosen consolidated groups.

This diploma thesis is focused on an evaluation of existence of synergic effects within consolidating subjects. Consolidated groups are defined by theoretical knowledge. Suitable indicators for evaluation of achieved synergic effects are suggested in the next step. Synergic effects are being observed by comparison of parent undertaking and consolidated group in chosen sphere. Based on an analysis, results are evaluated if the synergic effects are achieved.