Orientador: Cláudia Valéria Seullner Brandão / Resumo: O objetivo deste estudo foi comparar e avaliar a aplicação de célula tronco mesenquimal de tecido adiposo (CTM-TA) e plasma rico em plaquetas aquecido alogênico (PRP-AA) perilacrimal em cães com ceratoconjuntivite seca (CCS), bem como, se há diferença na resposta segundo o grau de gravidade da afecção. Foram analisados 20 cães com produção lacrimal <15mm/min e distribuídos aleatoriamente em dois grupos (n=10). O grupo 1 tratado com CTM-TA (5x106 e 3x106 células) (GCTM-TA) e com grupo 2 PRP-AA (0,7mL e 0,3mL) (GPRP-AA), ambos injetados perilacrimal nas glândulas lacrimal principal e da terceira pálpebra, respectivamente. Todos os olhos foram avaliados em quatro momentos (M0 M15, M30, M60 dias). As variáveis avaliadas foram: osmolaridade da lágrima; teste lacrimal de Schirmer (TLS); sensibilidade corneal; tempo de ruptura do filme lacrimal (TRFL); pressão intraocular; espessura corneal; e biopsia da conjuntiva bulbar, além das variáveis clínico-oftalmológicas. Houve melhora nos dois grupos a partir de M15 (p<0,05) na qualidade do filme lacrimal, avaliada por meio da osmolaridade e TRFL, sem diferença entre os grupos. A produção de lágrima aumentou, entretanto notou-se diferença significativa nos animais com CCS discreta no GPRP-AA a partir do M30, e nos cães com CCS grave no GCTM-TA a partir do M30 e no GPRP-AA a partir do M60. Verificou-se melhora dos sinais clínicos da inflamação em ambos os grupos. Aplicação única perilacrimal de CTM-TA e PRP aquecido alogênicos normaliza a ... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: The aim of the study was to compare and evaluate the application of allogenic mesenchymal stem cell adipose tissue derived (MSCs-AD) and inativate platelet-rich plasma allogenic (PRP-IA), perilacrimal in dogs with keratoconjunctivitis sicca (KCS), and if there is difference in the response according to the degree of severity of the condition. Twenty dogs with lacrimal production <15mm/min randomly assigned to two groups were used. Group treated with MSCs-AD (5x106 and 3x106) (GMSCs-AD) and with PRP-IA (0.7ml and 0.3ml) (GPRP-IA), both intralacrimal in the main lacrimal glands and third eyelid, respectively. The eyes were evaluated in four moments after application (M0 M15, M30, M60). The variables evaluated were: tear osmolarity e; Schirmer's tear test (STT); sensitivity corneal; tear film break time (TBUT); intraocular pressure; corneal thickness; and biopsy of the bulbar conjunctiva, in addition to clinical-ophthalmologic variables. There was improvement in two groups at M15 (p <0.05) on tear film quality, assessed by osmolarity and TBUT, with there was no difference between groups. STT increased, however, statistical difference was only observed in animals with mild KCS in the GPRP-IA from the M30 and in the intense KCS at M30 in the G MSCs-AD and at M60 in the GPRP-IA. There was an improvement in the clinical signs of inflammation in both groups. The unique Intralacrimal application of MSCs-AD and PRP-IA normalizes tear film quality and improves clinical signs of inflamma... (Complete abstract click electronic access below) / Doutor

Syfte: Syftet med studien var att utvärdera NIBUT (Non-Invasive Break-Up Time) och TBUT (Tear Break-Up Time) hos heltidsstuderande vid Linnéuniversitetet samt bidra till en ökad förståelse av ögonhälsan hos denna grupp. Metod: Tårfilmens stabilitet kontrollerades med NIBUT och TBUT, tiden mättes direkt efter en blinkning till första uppsprickningen av tårfilmens yta eller ny blinkning. NIBUT utfördes icke invasivt i Sirius och TBUT mättes i biomikroskop med fluorescein. Deltagarna fick inledningsvis svara på en enkät som gjorde det möjligt att analysera insamlad data utifrån ålder, kön, termin, skärmtid och poäng på DEQ-5 (5-Item Dry Eye Questionnaire). Svaren från DEQ-5 möjliggjorde att resultaten från NIBUT och TBUT mätningarna kunde jämföras mot upplevda symtom på torra ögon. Resultat: Studien inkluderade 36 studenter i åldersspannet 18–39 år (medianålder 23 år, IQR 5,25 år). NIBUT och TBUT visade en positiv signifikant korrelation (p &lt;0,001). En kortare TBUT korrelerade negativt med en högre totalpoäng på DEQ-5 (p = 0,004) samt antalet terminer som studerats (p = 0,025). NIBUT visade inte några liknande samband. Referensvärdet för torra ögon diagnostisering med hjälp av NIBUT och TBUT är en tid &lt;10 sekunder. Över hälften av deltagarna i studien, NIBUT 58% och TBUT 53%, hade en nedsatt break-up time &lt;10 sekunder. Vilket kan vara en indikation för torra ögon problematik. Slutsats: Denna studie visar att det finns ett signifikant samband mellan NIBUT och TBUT mätningarna. Kortare TBUT har visat en signifikant korrelation med högre totalpoäng på DEQ-5, likaså har signifikanta samband mellan TBUT och fler lästa terminer konstaterats. Trots korrelationen mellan NIBUT och TBUT påverkades resultaten av skillnader mellan de två mätmetoderna. / Purpose: The purpose of the studies was to evaluate NIBUT (Non-Invasive Break-Up Time) and TBUT (Tear Break-Up Time) in full-time students at Linnaeus University and to contribute to an increased understanding of the eye health of this group. Method: The stability of the tear film was checked with NIBUT and TBUT, the time was measured directly after a blink to the first break-up of the tear film surface or a new blink. NIBUT was performed non-invasively in Sirius and TBUT was measured in a biomicroscope with fluorescein. The participants initially had to answer a questionnaire that made it possible to analyze collected data based on age, sex, number of semesters studied, screen time and score on the DEQ-5 (5-Item Dry Eye Questionnaire). The responses from the DEQ-5 allowed the results from the NIBUT and TBUT measurements to be compared against perceived symptoms of dry eye. Results: The study included 36 students in the age range 18–39 years (median age 23 years, IQR 5.25 years). NIBUT and TBUT showed a positive significant correlation (p &lt;0.001). A shorter TBUT correlated negatively with a higher DEQ-5 total score (p = 0.004) as well as the number of semesters studied (p = 0.025). NIBUT did not show any similar correlations. The reference value for dry eye diagnosis using NIBUT and TBUT is a time &lt;10 seconds. Over half of the participants in the study, NIBUT 58% and TBUT 53%, had a reduced break-up time &lt;10 seconds. Which can be an indication of dry eye problems. Conclusion: This study shows that there is a significant connection between the NIBUT and TBUT measurements. Shorter TBUT has shown a significant correlation with higher total scores on the DEQ-5, likewise significant connections between TBUT and more semesters read have been established. Despite the correlation between NIBUT and TBUT, the results were influenced by differences between the two measurement methods.