Water resources for outdoor areas, such as lawns and landscapes, continues to become limited in many urban areas, especially in times of acute drought stress. Lawn species selection and cultural practices, such as mowing height, can strongly influence overall seasonal water needs. While previous research has reported various lawn species water use rates and differences in the ability of some cultivars to maintain green coverage during acute drought stress, little is known regarding the irrigation requirements of cool-season lawn species when using a deficit irrigation strategy based on a green coverage target threshold (e.g. 60-80% green) approach. Two greenhouse studies were conducted to screen various candidate species and seed mixtures in a sandy media. The highest water use and worst appearance/green coverage was associated with an inexpensive commercial lawn mixture; and the lowest water use and best appearance was generally associated with improved Kentucky bluegrass (<i>Poa pratensis</i> L.: KBG) cultivars. Field studies were conducted to quantify the irrigation requirements of drought susceptible (DS) and improved, drought tolerant (DT) KBG and tall fescue [<i>Schedonorus arundinaceus</i> (Schreb.): TF] cultivars, blends and mixtures at two mowing heights (5.1 or 8.9 cm). Results from a 74-day field study using a deficit irrigation replacement approach with a 70% green coverage threshold (GCT₇₀) irrigation trigger, demonstrated water savings of approximately 73 to 78% when using a DT TF (60.3 mm) as compared to 100% evapotranspiration (ET­_o) replacement (223.4 mm) and a conventional lawn irrigation approach (268.5 mm), respectively. The time to reach the GCT₇₀ generally ranked: TF=TF:KBG mixture>KBG and ranged from 18.0 days for DS ‘Right’ KBG and 52.5 days for DT ‘RainDance’ TF. Among TF and KBG cultivars using the GCT­₇₀irrigation approach, DT TF required 35 to 68% less supplemental irrigation compared to DT and DS KBG cultivars (92.1 vs. 187.3 mm), respectively. Within KBG cultivars, the DT ‘Desert Moon’ required one-half the irrigation of DS Right (92.1 vs. 187.3 mm), while there were no differences among TF cultivars for irrigation needs. Mowing height did not affect KBG irrigation needs, but TF at 5.1 cm showed increased visual quality and green coverage, and significantly reduced irrigation requirements. Field research also compared species mixtures and blends using DS and DT KBG and TF to determine the amount of a DT species/cultivar that would enhance drought performance with ratios ranging from 25-100% DT as well as 90:10 TF:KBG mixtures. The quantity of a DT KBG in a blend, and DT TF in a TF:KBG mixture reduced irrigation needs, whereas the drought rating of the KBG cultivar in a TF:KBG mixture had no significant effect. In summary, these studies continue to demonstrate that significant supplemental lawn irrigation savings can be achieved by the selection of superior DT species and cultivars combined with a deficit irrigation replacement approach compared to other cool-season species and conventional irrigation practices.

Thesis (M.S.)--Mississippi State University. Department of Entomology and Plant Pathology. / Title from title screen. Includes bibliographical references.

Grasslands comprise the greatest biome by land area, are sensitive to environmental factors affected by climate change, and can impact future climate change through their ability to store and release greenhouse gasses (GHGs). I performed two studies: 1) evaluated the effects of increased temperature and precipitation on slug herbivory/abundance and pasture forage production; 2) quantified different homeowner lawn system effects on soil-to-atmosphere GHG emissions. Climate change will likely affect pasture forage production, with implications for slug herbivory and abundance. I found little evidence that slugs have or will have significant effects on pasture production or plant community. Warming altered the abundance of slugs and modified seasonal trends, increasing slug abundance in spring/winter and reducing it in late-summer/fall, through both direct effects and changes in plant community and forage quality. Home lawns vary in levels of management, influencing the exchange of GHGs. I quantified the effects of three common home lawn systems of central Kentucky on GHG emissions, but found no significant differences in CO2, N2O, and NH3 fluxes. My research suggests that slug herbivory is not a dominant ecological process in Kentucky pastures and that common home lawn systems have similar soil-to-atmosphere GHG emissions.

As water demand increases it will become more imperative for golf course superintendents, landscape managers, and other industry professionals to improve water use efficiency in the management of recreational turfgrass. Scheduling irrigation according to actual turfgrass evapotranspiration rates (ETT) is an integral component of efficient irrigation practices. Impracticality of field derived ETT for industry use, however, directs the need of weather station derived reference (predicted) evapotranspiration (ET0). To accurately predict (estimate) ETT of turf and other crops, scientifically derived landscape (crop) coefficients (Kc values) are used in conjunction with mathematical models that incorporate local meteorological data. Research is limited, however, in identifying Kc values and subsequent ET0 for turfgrass species selected and maintained under high intensity recreational practices congruent of golf courses and sports fields in the cool-humid northeast climate. Therefore, objectives of this study were to (i) observe and record ETT of three commonly selected recreational turfgrass species; 'Exacta' Perennial ryegrass (Lollium perenne L.), 'Touchdown' Kentucky bluegrass (Poa pratensis L.), and 'Memorial' Creeping bentgrass (Agrostis stolinifera L.) maintained as golf and sports turf, (ii) analyze the impact various management practices (nitrogen fertility and height of cut) have on ETT, (iii) develop accurate Kc values appropriate for use with the recommended FAO 56 Penman-Monteith mathematical model for accurate ET0 of recreational turf maintained in the cool-humid northeast. Four heights of cut (HOC) and two nitrogen fertility rates (N) were evaluated to determine their impact on turfgrass growth and subsequent water use and ETT of three recreational turfgrass species. Golf turf (creeping bentgrass) maintained at a lower height of cut than sports turf exhibited a smaller leaf area component and a significantly lower (20%) ETT. N applied as slow release (82%) throughout the growing season increased ETT by 5%, particularly with perennial ryegrass sports turf. Taller HOC also increased ETT by 10% due to increased leaf area indices and subsequent decreased resistance to ET. Predicted ET0 according to FAO 56 for all three years of the study (79 observations) captured 71% of ETT. Yearly and monthly calculations suggest less variable (cloudy) weather yielded more accurate ET0. Crop coefficient (Kc) values established in conjunction with FAO 56 ET0 ranged from 0.90 to 1.00 for shorter golf course turf (creeping bentgrass), and 1.15 to 1.25 for taller sports turf (Kentucky bluegrass and perennial ryegrass). Results indicate shorter grass exhibits a lower ETT than taller grass due to various factors, and in the case of industry application, FAO 56 ET0 can accurately estimate ETT of recreational turf in the cool-humid northeast when fitted with appropriate Kc values.

Insect species complexes challenge entomologists in a variety of ways ranging from quarantine protection to pest management. Billbugs (Coleoptera: Curculionidae: <i>Sphenophorus</i> spp. Schönherr) represent one such species complex that has been problematic from a pest management perspective. These grass-feeding weevils reduce the aesthetic and functional qualities of turfgrass. Sixty-four species of billbugs are native to North America, and at least ten are associated with damage to turfgrass. Billbug species are sympatric in distribution and their species composition and seasonal biology varies regionally. Since their management relies heavily on proper choice of insecticide active ingredients and timing of insecticide applications that target specific life stages, understanding billbug seasonal biology underpins the development of efficient management programs. However, billbug seasonal biology investigations are currently hindered by our inability to identify the damaging larval stage to species level. DNA barcoding, which involves the use of short DNA sequences that are unique for each species, represents one potential tool that can aid these efforts. By combining DNA-based species identification with morphometric measures capable of serving as a proxy of larval development, it may be possible to gain a more holistic understanding of billbug seasonal biology. In this study, we developed a DNA barcoding reference library using cytochrome oxidase subunit 1 (COI) sequences from morphologically identified adult billbugs collected across Indiana, Missouri, Arizona, and Utah. Next, we applied our reference library for comparison and identification of unknown larval specimens collected across the growing season in Utah and Indiana. We then used a combination of DNA barcoding and larval head capsule diameters acquired from samples collected across a short span of the growing season to produce larval phenology maps. Adult billbug COI sequences varied within species, but the variation was not shaped by geography, indicating that this locus itself could resolve larval species identity. Overlaid with head capsule diameter data from specimens collected across the growing season, a better understanding of billbug species composition and seasonal biology emerged. This knowledge will provide researchers with the tools necessary to fill critical gaps in our understanding of billbug biology thereby improving turfgrass pest management. Using this approach researchers will be able to support efforts to provide growers with the information necessary to develop more prescriptive, location-based management programs and reduce the ecological footprint of turfgrass pest management.

Plants require N to complete their life cycle. Without adequate concentration of N, crops will not produce their potential yields. For turfgrass systems, N fertilizer application allows for the maintenance of functional, aesthetic, and recreational properties. However, fertilizer mismanagement is common and leads to N pollution in the environment. Controlled-release and slow-release fertilizers can enhance nitrogen (N)-use efficiency, reduce N pollution, minimize the need for repeated fertilizer applications, and reduce turfgrass shoot growth and associated costs. In order to evaluate the effectiveness of these fertilizers in the Intermountain West, research is needed. The timing of N release was evaluated for seven urea fertilizers: uncoated, sulfur coated (SCU), polymer-sulfur coated (PSCU), and four polymer-coated (PCU) with release timings of 45, 75, 120, and 180 d estimated release. These products were placed on bare soil, a Kentucky bluegrass (Poa pratensis L.) thatch layer, and incorporated into soil. These three placement treatments were replicated to allow for enough samples to be placed in two locations. The first was outside in a field to represent field conditions with diurnal fluctuating temperatures and the second was placed in a storage facility to replicate laboratory conditions with static diurnal temperatures. The PCU prills incorporated into soil under field conditions generally released N over the estimated release period. However, when applied to bare soil or thatch, N from PCU had 80% or greater N release by 35 d after application regardless of expected release time. Fertilizers under laboratory conditions had minimal N release despite having similar average daily temperatures, suggesting that fluctuating temperatures impact N release. The PSCU and SCU treatments were no different from uncoated urea, showing no slow release properties for this particular product. Spring-applied N fertilizer trials were conducted over two years to determine the optimal N rate for Kentucky bluegrass. Similar PCU120 products were applied at 50, 75, and 100% of the recommended full rate, while also being compared to an unfertilized control and urea applied either all at once or split monthly. Spring-applied PCU showed minimal initial N response while urea applied all at once resulted in an initial spike of N uptake. Once PCU began to release N, there was minimal difference for all rates compared to urea split monthly for biomass growth, verdure, and shoot tissue N. Although at the 50% rate, there were a few sampling dates with slower growth and lower verdure. The decrease in verdure at this low rate was slight, and it is recommended that PCU could be applied effectively at a reduced rate between 50 and 75%. Although for better results, additional quick release N is required to compensate for early season lag in N release.

Sclerotinia homoeocarpa (F.T. Bennett) is one of the most economically important pathogens on high amenity cool-season turfgrasses where it causes dollar spot. Due to decades of over-reliance and repeated chemical treatments, S. homoeocarpa has developed resistance and insensitivity to multiple classes of fungicides. To understand the genetic mechanisms of fungicide resistance, the whole genomes of two strains with varying resistance levels to fungicides, were sequenced. In unpublished data (Sang et al.), a RNA-sequencing analysis revealed three CYP450s that were validated to play a functional role in S. homoeocarpa’s resistance against different fungicide classes. We also identified CYP450 metabolic action on the multi-site mode of action fungicide chlorothalonil. Chlorothalonil is an extensively used contact fungicide and has been known to be persistent in soils. Yet, S. homoeocarpa resistance to chlorothalonil has not been reported in the field. High Performance Liquid Chromatography (HPLC) indicated faster rates of chlorothalonil biotransformation by CYP450 overexpression strains when compared to the wild-type. We show by GC-MS that the primary transformation intermediate found in soils, 4-hydroxy-2,5,6 trichloroisophthalonitrile is produced by CYP450s’ metabolism.

A change in surface condition of a watershed, which is usually caused by development, can have measured effects on the naturally occurring hydrologic cycle and nitrogen cycle. This could result in environmental problems, such as reduced springflow and eutrophication. In an effort to address these issues, a combination of best management practices (BMPs) can be adhered to. The practice of using excess stormwater as a source for irrigation is proposed as a BMP for the minimization of impacts by development to the hydrologic and nitrogen cycles. To study the proposed BMP, a field experiment was installed in an outdoor location on the UCF main campus in Orlando, Florida. The experiment consists of three soil chambers, (2x2x4 ft, L:W:H), filled with compacted soil and covered with St. Augustine grass to simulate a suburban lawn. The grass was irrigated up to twice a week with detained stormwater with a nitrate nitrogen concentration of up to 2 mg/L. A mass balance and a total nitrogen balance were performed to determine evapotranspiration (ET) and impacts on groundwater nitrogen content. It was determined that the groundwater characteristics are largely dependent on the characteristics of the soil. The input nitrogen (precipitation and irrigation) was mostly in the form of nitrate and the output nitrogen (groundwater) was mostly in the form of ammonia. A total nitrogen mass balance indicated the mass output of nitrogen was significantly larger than mass input of nitrogen, which was due to ammonia leaching from the soil. Only small concentrations of nitrate were detected in the groundwater, resulting in an estimated nitrate removal (conversion to ammonia) of 97 percent at a depth of four feet when the input nitrate concentration was 2 mg/L. The average ET of the three chambers was compared to the estimated ET from the modified Blaney-Criddle equation on a monthly basis and a yearly basis. The modified Blaney-Criddle equation was proven to be accurate for estimating the actual ET for this application: irrigated St. Augustine grass in the Central Florida climate. In conclusion, using the available literature and the data collected from the field experiment, it was shown through an example design problem that the proposed BMP of using excess stormwater as a source for irrigation can help achieve a pre- versus postdevelopment volume balance and can help control post-development nitrate emissions.

Buffalograss [Buchloe dactyloides (Nutt.) Engelm.] is a warm season, perennial grass native to the Great Plains from southern Canada to Mexico (Beetle, 1950). This newly developed, low input, turf-type grass is recommended for use on low maintenance sites (Falkenberg-Borland and Butler, 1982; Pozarnsky, 1983; Wu and Harivandi, 1989; Shearman et al., 2005). Recently, the use of buffalograss as a turfgrass has increased due to its drought tolerance, low nutrient requirements, and low growing height (Harivandi and Wu, 1995; Frank et al., 2004). It is an excellent choice in California where water use is limited. Unsightly winter dormancy of buffalograss can be overcome by growing mixtures of buffalograss and fine fescue (Festuca spp.). Overtime species composition can be overtaken by the fine fescue, unintentionally converting the mixed turfgrass stand to a fine fescue monostand (Severmutlu, et al., 2005). Research on buffalograss establishment in fine leaved fescues from seed or by vegetative methods was completed from 2007 to 2009 at the California Polytechnic State University Horticulture Unit in San Luis Obispo, California. Comparisons were made between mixtures of eight cultivars of buffalograss (Prairie, Prestige, UC Verde, 609, Bowie, Cody, Texoka, and Bison) and three fine leaved fescue species [hard fescue (Festuca trachyphylla Thuill.), sheep fescue (Festuca ovina L), and red fescue (Festuca rubra L.)] to determine which combination and establishment strategy provides the highest quality turf for the California central coast region. Evaluations made on buffalograss establishment and competitive ability when grown in pre-existing fine fescue turfgrasses showed seeded cultivars (Bowie, Cody, Texoka, and Bison) were unsuccessfully established (zero percent coverage in two growing seasons), and vegetative cultivar (Prairie, Prestige, 609 and UC Verde) establishment was greatly dependent on the type of cultivar planted. After two growing seasons, buffalograss cultivar UC Verde had the highest establishment rate (38.6 percent living ground cover) and Prestige had the lowest (11.4 percent living ground cover). Results from this study do not recommend establishing seeded buffalograss cultivars into pre-existing fine leaved fescue turfgrass stands. Vegetative buffalograss cultivars can be established into pre-existing fine leaved fescue turfgrass stands; however, this process is too slow for most turfgrass practitioners and is quite unsightly in winter dormancy during the establishment process.

Sustainable weed control in managed agricultural systems requires the judicious use of multiple weed control tactics and prevents over-reliance on any one tactic. In this context, sustainable weed management plays a critical role in the mitigation of one of agriculture's most pressing problems- herbicide resistance. Research conducted in Virginia sought to explore the effects of integrating multiple weed management tactics in corn and cool-season turfgrass. Additionally, research was conducted to confirm nicosulfuron and glyphosate herbicide resistance in Virginia johnsongrass and elucidate the molecular mechanisms conferring those resistances. Rye and hairy vetch cover crop residues, combined with reduced rates of preemergence herbicide and postemergence glyphosate applications, were shown to provide sufficient weed control and corn yield. Cover crop type or residue level did not augment weed control in corn production systems, but the use of glyphosate was essential for late-season weed control. Rye and vetch biculture as a cover crop increased corn yield compared to rye cover crop alone. In cool-season turfgrass, the addition of reduced preemergence herbicide rates to corn gluten meal, an organic herbicide product, reduced crabgrass 25%. Moreover, control was dependent on herbicide choice. Herbicides applied at half of recommended labeled rates or less did not control crabgrass at a commercially-acceptable level, regardless of corn gluten meal addition. In field experiments, Virginia johnsongrass expressed resistance to nicosulfuron and glyphosate. Glyphosate at 0.88 kg ae ha-1 controlled johnsongrass 65%. Nicosulfuron at 0.14 kg ai ha-1 controlled the same population 10%. Greenhouse experiments confirmed differential sensitivity of putative herbicide-resistant johnsongrass seedlings to nicosulfuron and glyphosate when compared to a susceptible population. Herbicide resistance was not conferred via target-site mutation. Five ALS-gene site mutations were confirmed absent in Virginia johnsongrass, while three others were located in coding regions that could not be elucidated in johnsongrass. Further investigations showed glyphosate resistance was not conferred via reduction in herbicide absorption or translocation. The susceptible johnsongrass caused an increase in a polar metabolite at Rf = 0.17 with concomitant reduction in glyphosate over time. Although the mechanism is not clear, these data suggests that glyphosate resistance in johnsongrass may be associated with differential metabolism. / Ph. D.