Tendinopatias são distúrbios comuns com uma variedade de tratamentos e terapias e a Laserterapia aparece como uma alternativa no tratamento destas doenças. Nós investigamos os efeitos da irradiação laser no modelo de tendinite induzida por colagenase, em tendões calcâneo de ratos, verificando sua ação em importantes marcadores inflamatórios. Métodos: Foram utilizados ratos machos (Wistar) divididos em grupos: Salina e tendinite tratados com Diclofenado de Sódio ou Laser (1J, 3J ou 6J). A tendinite foi induzida por colagenase (100<font face=\"Symbol\">mg/tendão) sobre o tendão calcâneo que foi removido para análises. Foi verificado a expressão de COX-1, 2, TNF-a, TGF-<font face=\"Symbol\">b, IL-10, MMP-3, 9 e 13 (RT-PCR), a produção de PGE2 (ELISA) e a expressão de NT, COX-2 e NK-1 (Western Blotting). Resultados e discussão: A laserterapia (810nm, 100mW, 3 J), utilizada no tratamento da tendinite induzida por colagenase em tendão calcâneo de ratos, foi eficaz na redução de importantes marcadores inflamatórios, tornando-se uma ferramenta promissora para o tratamento de lesões tendíneas. / Tendinopathy is a common disease with a variety of treatments and therapies. Laser therapy appears as a alternative treatment. We research the effects of the laser irradiation in tendinitis model induced by collagenase injection on Achilles tendon rats, verified its action in important inflammatory markers. Methods: The male rats (Wistar) were used and divided in groups: Saline and tendinitis, treated with Sodium diclofenac or Laser (1J, 3J or 6J). The tendinitis was induced by collagenase (100<font face=\"Symbol\">mg/tendon) on the Achilles tendon which was removed for analyzes. The genic expression for COX-1, 2; TNF-a, TGF-<font face=\"Symbol\">b; IL-10; MMP-3, 9, 13 (RT-PCR); PGE2 production (ELISA kits) and NT, COX-2, NK-1 expression (Western Blotting) was measured. Results and discussion: The Laser therapy (810nm, 100mW, 3 J) used in the treatment of the tendinitis induced by collagenase in Achilles tendon in rats, was effective in the reduction of important inflammatory markers, becoming a promising tool for the treatment of tendon diseases.

Tendinopatias são distúrbios comuns com uma variedade de tratamentos e terapias e a Laserterapia aparece como uma alternativa no tratamento destas doenças. Nós investigamos os efeitos da irradiação laser no modelo de tendinite induzida por colagenase, em tendões calcâneo de ratos, verificando sua ação em importantes marcadores inflamatórios. Métodos: Foram utilizados ratos machos (Wistar) divididos em grupos: Salina e tendinite tratados com Diclofenado de Sódio ou Laser (1J, 3J ou 6J). A tendinite foi induzida por colagenase (100<font face=\"Symbol\">mg/tendão) sobre o tendão calcâneo que foi removido para análises. Foi verificado a expressão de COX-1, 2, TNF-a, TGF-<font face=\"Symbol\">b, IL-10, MMP-3, 9 e 13 (RT-PCR), a produção de PGE2 (ELISA) e a expressão de NT, COX-2 e NK-1 (Western Blotting). Resultados e discussão: A laserterapia (810nm, 100mW, 3 J), utilizada no tratamento da tendinite induzida por colagenase em tendão calcâneo de ratos, foi eficaz na redução de importantes marcadores inflamatórios, tornando-se uma ferramenta promissora para o tratamento de lesões tendíneas. / Tendinopathy is a common disease with a variety of treatments and therapies. Laser therapy appears as a alternative treatment. We research the effects of the laser irradiation in tendinitis model induced by collagenase injection on Achilles tendon rats, verified its action in important inflammatory markers. Methods: The male rats (Wistar) were used and divided in groups: Saline and tendinitis, treated with Sodium diclofenac or Laser (1J, 3J or 6J). The tendinitis was induced by collagenase (100<font face=\"Symbol\">mg/tendon) on the Achilles tendon which was removed for analyzes. The genic expression for COX-1, 2; TNF-a, TGF-<font face=\"Symbol\">b; IL-10; MMP-3, 9, 13 (RT-PCR); PGE2 production (ELISA kits) and NT, COX-2, NK-1 expression (Western Blotting) was measured. Results and discussion: The Laser therapy (810nm, 100mW, 3 J) used in the treatment of the tendinitis induced by collagenase in Achilles tendon in rats, was effective in the reduction of important inflammatory markers, becoming a promising tool for the treatment of tendon diseases.

Submitted by Nadir Basilio (nadirsb@uninove.br) on 2015-07-20T18:29:39Z No. of bitstreams: 1 Romildo Torres da Silva.pdf: 1197534 bytes, checksum: 02ae9c343d597dbbc37f2a71f43ad453 (MD5) / Made available in DSpace on 2015-07-20T18:29:39Z (GMT). No. of bitstreams: 1 Romildo Torres da Silva.pdf: 1197534 bytes, checksum: 02ae9c343d597dbbc37f2a71f43ad453 (MD5) Previous issue date: 2015-12-12 / Tendinopathy is a common disease with a variety of treatments and therapies. Laser therapy appears as a alternative treatment. We research the effects of the laser irradiation in tendinitis model induced by collagenase injection on Achilles tendon rats, verified its action in important inflammatory markers. Methods: The male rats (Wistar) were used and divided in groups: Saline (C), Tendinitis (NT) and tendinitis treated with Sodium diclofenac (D) or Laser (1J) and (3J). The tendinitis was induced by collagenase (100μg/tendon) on the Achilles tendon which was removed for analyzes. The gene expression for COX-2; TNF-α; il-6 and IL-10 (RT-PCR) was measured. Results and discussion: The Laser therapy (660nm, 100mW, 3J) used in the treatment of the tendinitis induced by collagenase in Achilles tendon in rats, was effective in the reduction of important inflammatory markers, becoming a promising tool for the treatment of tendon diseases. / Tendinopatias são distúrbios comuns com uma variedade de tratamentos e terapias e a Laserterapia aparece como uma alternativa no tratamento destas doenças. Nós investigamos os efeitos da irradiação laser no modelo de tendinite induzida por colagenase, em tendões calcâneo de ratos, verificando sua ação em importantes marcadores inflamatórios. Métodos: Foram utilizados ratos machos (Wistar) divididos em grupos: Controle (C), Tendinite Não Tratado (NT), Tratamento com Diclofenado de Sódio (D) ou Laser (1J) e (3J). A tendinite foi induzida por colagenase (100μg/tendão) sobre o tendão calcâneo que foi removido para análises. Foi verificado a expressão gênica de COX-2, TNF-α, IL-6 e IL-10 (RT-PCR). Resultados e discussão: A laserterapia (660nm, 100mW, 3J), utilizada no tratamento da tendinite induzida por colagenase em tendão calcâneo de ratos, foi eficaz na redução de importantes marcadores inflamatórios, tornando-se uma ferramenta promissora para o tratamento de lesões tendíneas.

Submitted by Nadir Basilio (nadirsb@uninove.br) on 2018-06-21T18:42:55Z No. of bitstreams: 1 Heliodora Leao Casalechi.pdf: 2679810 bytes, checksum: f981677bdc05834caca5e26029e42566 (MD5) / Made available in DSpace on 2018-06-21T18:42:55Z (GMT). No. of bitstreams: 1 Heliodora Leao Casalechi.pdf: 2679810 bytes, checksum: f981677bdc05834caca5e26029e42566 (MD5) Previous issue date: 2015-06-24 / Tendinitis is a common disorder of the musculoskeletal system with multiple pathological manifestations. Although most tendons have the ability to spontaneously repair after injury, scar tissue is formed that can prevent the tendon to perform normal functions. Studies have been conducted using phototherapy in different health areas, using low-intensity lasers and LEDs. However, there are few studies with LEDs therapy. The aim of this study was to investigate the effect of phototherapy with Laser (830nm) and LED (945nm) in the repair process of the calcaneus tendonitis in rats. The inflammation was induced by controlled contusion in the medial region of the Achilles tendon of the animals. Was used 112 young male Wistar rats (240 ± 20g), divided into 14 groups with two different experimental periods. Groups CONT (control group), TEND (tendinitis group) during periods 7 and 14 days, LEDT (LED therapy, from th to 7th day and from 1th to 14th day), and LEDT delay (LED therapy from 7th to 14th day). The therapy was initiated 12h after the tendinitis induction, with a 48h interval between irradiations (dose 6J/point). The control animals received simulation of treatment; the same experimental design was used for Laser treatment. All groups were euthanized on the 7th or 14th day after the induction. The tendons were dissected, extracted, and sent for analysis. Were performed biomechanical and histological analysis to evaluate neutrophils and collagen, were also quantitated the inflammatory mediators by real-time polymerase chain reaction (RT-PCR) and the IL-6 and TNF- α levels by ELISA. The results showed that treatments with low-intensity lasers in the parameters used and the times studied reduces migration of inflammatory cells and improves the quality of repair, but the LED even decreasing the inflammation was not effective of biomechanical properties of tendon tissue. / A tendinite é uma desordem comum do sistema musculoesquelético. Embora os tendões tenham habilidade de reparação espontânea após ferimento, o tecido da cicatrização que é formado pode impedir o tendão de executar as funções normais. Estudos vêm sendo realizados utilizando a fototerapia em diferentes áreas da saúde, empregando tanto laseres quanto LEDs de baixa intensidade. Contudo, existe um número reduzido de estudos com a utilização da terapia com LEDs. O objetivo deste estudo foi investigar os efeitos anti-inflamatórios e as propriedades mecânicas do tecido após a ação da fototerapia utilizando LASER (830nm) e LEDs (945nm), na região do infravermelho, na tendinite induzida por trauma mecânico em tendão de Aquiles de ratos. Foram utilizados 112 ratos machos albinos da linhagem Wistar (240 ± 20g), distribuídos em 14 grupos e dois períodos experimentais diferentes. Grupos CONT (grupo controle), os grupos TEND (grupos tendinite) nos períodos de 7 e 14 dias, o LEDT (grupos terapia LED), do 1º ao 7º dia e do 1º ao 14º dia) e o grupo LEDT delay (terapia LED do 7º ao 14º dia). A terapia teve início 12 horas após a indução da tendinite, utilizando uma dose de 6J no ponto de aplicação, com intervalos de 48 em 48 horas. A fototerapia foi aplicada transcutaneamente em um único ponto sobre a região lesionada. Os animais dos grupos controle foram submetidos à simulação da aplicação com o aparelho desligado, o mesmo desenho experimental foi utilizado feito para o tratamento Laser. As eutanásias ocorreram ao 7º e 14º dia do experimento. Foi realizada análise biomecânica, histopatológica para avaliação de neutrófilos e colágeno, também foram quantificados os mediadores inflamatórios por meio da RT-PCR e os níveis de IL-6 e TNF- α foram avaliados pelo teste imunoenzimático (ELISA). Por meio dos resultados encontrados, é possível inferir que os tratamentos com Laser de baixa intensidade, nos parâmetros utilizados e nos tempos estudados melhora a qualidade do reparo do tecido tendíneo, a biomecânica e reduz a migração de células inflamatórias, já o LED de baixa intensidade apesar de diminuir a inflamação não foi eficiente na manutenção da biomecânica do tendão.

Title: The analysis of risk factors for tendinopathy in runners Objectives: The aim of this thesis is to find out the most important risk factor for development of Achilles tendinopathy, detect the most relevant diagnostic approach with preventive monitoring for Achilles tendinopathy and evaluate the best treatment for Achilles tendinopathy in runners. Methods: The diploma thesis is in form of literary review. The Scopus, PubMed and Web of science databases were used to search for studie using a combination of keywords. A total of 1084 titles were identified. Due to duplicates 402 titles were excluded. On the basis of the name of the article 51 titles were included. On the basis of the availability 50 titles were included. On the basis of the abstract 46 titles were included. Finally 29 titles were included in this review. Results: The most important risk factor for development of Achilles tendinopathy was training intensity for distances 1500-3000 m, week running volume >65 km or being new to running. The most relevant diagnostic approach for Achilles tendinopaty was clinical examination assessing pain (Achilles tendon pain, positive palpation test) and function (pain during physical aktivity) of Achilles tendon. This approach serves as indication for sonographic assessment of Achilles tendon...

Title Comparison between the effectiveness of extracorporeal shockwave therapy (ESWT) and the surgical approach in the treatment of the rotator cuff tendinopathy. Objective The main goal of this research work is comparing the effectiveness of extracorporeal shock wave therapy and the surgical intervention (arthroscopic approach) in the treatment of calcific rotator cuff tendinopathy depending on the outcome measures of the Constant-Murley Score (CMS), and the Visual Analogue Scale (VAS) from the recent scientific publications. Methodology This thesis is systemic (literature) review. The studies which recruited in this research were randomized controlled trials, prospective studies, and retrospective case series studies which written in English language form, and published between the year 2000 to the year 2018. The studies were retrieved from following databases: EMBASE, PubMed, CINAHL, Medline, SpringerLink, ProQuest using different integrations of the key words. The participants in the studies were from both genders and all ages. The type of the pathology which included in this research was chronic calcific rotator cuff tendinopathy. Regarding the intervention types both Radial, and Focused ESWT with low, medium, and high intensities and the arthroscopic interventions were the main types of...

Indiana University-Purdue University Indianapolis (IUPUI) / Tendinopathy is a common degenerative pathology that is characterized by activity related pain, focal tendon tenderness, intratendinous imaging changes, and typically results in changes in the histological, mechanical, and molecular properties of the tendon. Tendinopathy is difficult to study in humans, which has contributed to limited knowledge of the pathology, and thus a lack of appropriate treatment options. However, most believe that the pathology is degenerative as a result of a combination of both extrinsic and intrinsic factors. In order to gain understanding of this pathology, animal models are required. Because each tendon is naturally exposed to different conditions, a universal model is not feasible; therefore, an appropriate animal model must be established for each tendon susceptible to degenerative changes. While acceptable models have been developed for several tendons, a reliable model for the Achilles tendon remains elusive. The purpose of this dissertation was to develop an animal model of Achilles tendinopathy by investigating the individual and combined effects of an intrinsic and extrinsic factor on the rodent Achilles tendon. Rats selectively bred for high capacity running and Sprague Dawley rats underwent uphill treadmill running (an extrinsic factor) to mechanically overload the Achilles tendon or served as cage controls. Collagenase (intrinsic factor) was injected into one Achilles tendon in each animal to intrinsically break down the tendon. There were no interactions between uphill running and collagenase injection, indicating that the influence of the two factors was independent. Uphill treadmill running alone failed to produce any pathological changes in the histological or mechanical characteristics of the Achilles tendon, but did modify molecular activity. Intratendinous collagenase injection had negative effects on the histological, mechanical, and molecular properties of the tendon. The results of this dissertation demonstrated that the combined introduction of uphill treadmill running and collagenase injection did not lead to degenerative changes consistent with human Achilles tendinopathy. Intratendiouns collagenase injection negatively influenced the tendon; however, these changes were generally transient and not influenced by mechanical overload. Future studies should consider combinations of other intrinsic and extrinsic factors in an effort to develop an animal model that replicates human Achilles tendinopathy.