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1	Verhalten von ausgewählten Tetrazyklinen und Sulfonamiden in Wirtschaftsdünger und in Böden / Behaviour of selected tetracyclines and sulfonamides in organic fertilizers and soils Engels, Heike 15 July 2004 (has links) No description available. 630 Landwirtschaft Veterinärmedizin Agricultural Sciences Tierarzneimittel Antibiotika Tetrazykline Sulfonamide Wirtschaftdünger Gülle Sickerwasser animal drugs antibiotics tetracyclines sulfonamides organic fertilizer liquid manure leachate 48.00
2	Applicability of a computational design approach for synthetic riboswitches Domin, Gesine, Findeiß, Sven, Wachsmuth, Manja, Will, Sebastian, Stadler, Peter F., Mörl, Mario 25 January 2017 (has links) (PDF) Riboswitches have gained attention as tools for synthetic biology, since they enable researchers to reprogram cells to sense and respond to exogenous molecules. In vitro evolutionary approaches produced numerous RNA aptamers that bind such small ligands, but their conversion into functional riboswitches remains difficult. We previously developed a computational approach for the design of synthetic theophylline riboswitches based on secondary structure prediction. These riboswitches have been constructed to regulate ligand dependent transcription termination in Escherichia coli. Here, we test the usability of this design strategy by applying the approach to tetracycline and streptomycin aptamers. The resulting tetracycline riboswitches exhibit robust regulatory properties in vivo. Tandem fusions of these riboswitches with theophylline riboswitches represent logic gates responding to two different input signals. In contrast, the conversion of the streptomycin aptamer into functional riboswitches appears to be difficult. Investigations of the underlying aptamer secondary structure revealed differences between in silico prediction and structure probing. We conclude that only aptamers adopting the minimal free energy (MFE) structure are suitable targets for construction of synthetic riboswitches with design approaches based on equilibrium thermodynamics of RNA structures. Further improvements in the design strategy are required to implement aptamer structures not corresponding to the calculated MFE state. Genexpression Galactosidase Bindungsprotein Proteinstruktur Streptomyzin Tetrazykline Theophyllin RNA Molekül gene expression galactosidase ligands logic protein structure secondary streptomycin thermodynamics tetracycline theophylline rna escherichia coli free energy binding (molecular function) molecule ddc:572
3	Applicability of a computational design approach for synthetic riboswitches Domin, Gesine, Findeiß, Sven, Wachsmuth, Manja, Will, Sebastian, Stadler, Peter F., Mörl, Mario January 2016 (has links) Riboswitches have gained attention as tools for synthetic biology, since they enable researchers to reprogram cells to sense and respond to exogenous molecules. In vitro evolutionary approaches produced numerous RNA aptamers that bind such small ligands, but their conversion into functional riboswitches remains difficult. We previously developed a computational approach for the design of synthetic theophylline riboswitches based on secondary structure prediction. These riboswitches have been constructed to regulate ligand dependent transcription termination in Escherichia coli. Here, we test the usability of this design strategy by applying the approach to tetracycline and streptomycin aptamers. The resulting tetracycline riboswitches exhibit robust regulatory properties in vivo. Tandem fusions of these riboswitches with theophylline riboswitches represent logic gates responding to two different input signals. In contrast, the conversion of the streptomycin aptamer into functional riboswitches appears to be difficult. Investigations of the underlying aptamer secondary structure revealed differences between in silico prediction and structure probing. We conclude that only aptamers adopting the minimal free energy (MFE) structure are suitable targets for construction of synthetic riboswitches with design approaches based on equilibrium thermodynamics of RNA structures. Further improvements in the design strategy are required to implement aptamer structures not corresponding to the calculated MFE state. info:eu-repo/classification/ddc/572 ddc:572

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