The first objective of this thesis was compiling research about currently produced pistons for internal combustion engines and about additive manufacturing and based on the acquired information deciding which type of piston makes the most of the advantages. Following this research, the next step was creating a design adjustment of a piston, which was previously designed with the conventional methods of manufacturing in mind, in a way that would make the best use of the different possibilities of additive manufacturing. There was also an optimisation carried out, which depicted the densities of material elements in the piston, showing where the material was less important. There were three variants of the additive manufactured piston created, ranging from a relatively conservative design, which could be theoretically produced by conventional methods with a more substantial subtraction of material added, to a design which could only be produced via additive manufacturing. A thermo-structural analysis at maximum engine load was carried out for all these piston models. Based on these data, a conclusion was made, whether the use of additive manufacturing was justified over the use of the conventional subtractive methods.

The master‘s thesis deals in theoretical part with the casting classification, ductile iron casting, its inoculation and modification and quality evaluation using thermal analysis, image analysis and testing of mechanical properties. The aim of the practical part was to test the effects of preconditioning on the properties of ductile iron and to find out whether it is able to replace the current method of inoculation or to increase the mechanical properties of ductile iron castings. The state of the ductile iron was under control by spectral and thermal analysis. The mechanical properties and image analysis were checked on finished casting.

The aim of this work is to describe news in Autodesk Inventor Professional 2008. In second part of the work there are described the possibilities of technical drawing in Autodesk Inventor. In third part there is mentioned basic information about ANSYS Workbench. There is thermal simulation of asynchronous motor too.

In its first part this Master´s thesis describes creation of electronic database design in Raster Design 2009 program as an effective form of storage only for paper documentation. The second part of my thesis provides calculation of primary and secondary winding of current instrument transformer of over-current conduit through the winding and influence on other parts of transformer i.e. error of transformer or increasing magnetic induction core and others. The last third part is focusing on usage of ANSYS Workbench in power electrical engineering. The thesis shows specific example of warming of current instrument transformer (thermal analysis) and comparing the results with theoretical (calculated) values.

This work deals with design of thermal source of panel shape for scientific biological application. Panel has adjustable surface temperature and thermal gradient. The first part of this work concern with design and numerical modeling of the thermal system, application of materials and arrangement of thermal and cooling devices – Peltier elements. Second part of this work is about construction of the panel and experimental measurement.

This thesis is focus on thermal analysis of aircraft electronic devices and their cooling possibilities. The two analytic methods of thermal analysis are applied on two particular technical objects. The laboratory experiment of non-contact temperature measurement method is applied on real unit. The results of simulation are compared with results of experiment.

The subject of my master’s thesis is creating a computational model of a vertical turning centre frame. The frame is influenced by the heat generated in a motion system. The computational model was created to evaluate a temperature distribution in machine frame structure and thermal deformations caused by temperature gradients.

This diploma thesis investigated thermal stability and the degradation of hyaluronan (HA) in HA with a molecular mass of 90–130 kDa and in HA with a molecular mass of 1 500–1 750 kDa. The following methods were used for the research: rheology, SEC-MALLS, TGA and DSC. Low-molecular HA was subject to time dependency of degradation investigation, where it was dried at a temperature of 90 °C for a period of 30 minutes and 60 minutes prior to the preparation of the solutions itself. High-molecular HA was investigated not only from the point of view of time but from the point of view of temperature dependency of degradation as well. In the case of investigating the time dependency of degradation, high-molecular HA was dried at a temperature of 75 °C at a time range from 15 minutes to 120 minutes prior to the preparation of the solutions. During the preparation of the solutions for discovering the temperature dependency of degradation, the high-molecular HA was then dried for a period of 30 minutes at a temperature range from 60 °C to 90 °C. For low-molecular HA, thermal stability was proven. Therefore, there is no decrease in the molecular mass and the solutions did not demonstrate a significant decrease of viscosity. For high-molecular HA, thermal stability was not proven. Degradation due to the temperature of drying as well as the time of drying occurred, which was demonstrated by a significant decrease in molecular mass and viscosity of the solutions. While in the case of using a drying temperature of 60 °C, a decrease in the molecular mass occurred by approximately 5 %, the molecular mass decreased by approximately 20 % at a drying temperature of 90 °C compared to undried HA. Due to this reason, high-molecular HA was also further investigated by means of the TGA method, where the decrease of humidity of HA samples in relation to the drying temperature was observed. The DSC method was also used. The objective of the DSC method was to find out temperatures, at which evaporation of humidity contained in an HA sample in relation to its form (undried HA, dried HA and lyophilized HA) occurs. This method further finds out the heat necessary to evaporate humidity from an HA sample. To conclude this research, the results obtained for high-molecular HA were compared with the results of other drying processes – lyophilized proved to be a very gentle drying method because a decrease in the molecular mass for lyophilized HA compared with undried HA almost did not occur.

This diploma thesis deals with the utilization of physico-chemical analysis in study on microbial stress–response. The main content of this work was to propose and optimize techniques and methods which are generally used in different industries. Two bacterial strains, Cupriavidus necator H16 and its mutant strain Cupriavidus necator PHB-4 producing polyhydroxyalkanoates (PHA) under certain conditions, were used as model microorganisms. Initially, microscopic teques such as transmission electron microscopy (TEM), cryo scanning electron microscopy (cryo SEM) and atomic force microscopy (AFM) were proposed and tested for the characteriozation of morphological differences of bacteria. Furthermore, viscoelastic properties of bacteria were determined and compared by the oscillatory tests of the rheology method considering another type of samples. Thermal analysis methods, differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA) in particular, focused on water transport and behavior of intracellular water influenced by presence of the granules of PHB in bacteria. The last technique, utilized in the study, was dynamic and electrophoretic light dispersion during pH changes for characterization of the surface properties of bacteria such as size, zeta potential and the isoelectric point.

This diploma thesis in the theoretical part deals with classification of cast iron, problems of gray cast iron production, methods of inoculation and valuation of the gray iron quality by means of thermal analysis. The aim of practical part was to investigate effectiveness of inoculation of two different inoculants using thermal analysis. In terms of mechanical properties, hardness and ultimate tensile strength were measured. Metallographic specimen were evaluating by means of image analysis software.