Transverse cotyledonal cracking (TVC) was characterised at physiological, biochemical and molecular levels in three common bean (Phaseolus vulgaris L.) cultivars: Imbali, Tongaat and Tokai. The incidence of TVC was determined visually on ten-day old seedlings and was expressed as the number of cracks per plant. The effect of Ca++ on TVC incidence was examined by enhancing the calcium content of seed cotyledons using calcium salts in seed priming and coating. EDAX was used to quantify the cotyledonal calcium content. Activities of the pectinolytic enzymes polygalacturonase (PG) and pectin methylesterase (PME) were assayed at dry seed, VC, R4 and R6 phenophases of the bean plant, and zymogram electrophoresis was used to identify the two enzymes. The results showed that cultivars Imbali, Tongaat, and Tokai were susceptible resistant and intermediate, respectively, with respect to TVC incidence. Calcium uptake was higher in Tongaat cultivar (P= 0.05) compared with Imbali and Tokai, irrespective of calcium salt enhancement. Vigour was significantly reduced (P< 0.01) by both coating and priming. TVC significantly lowered yield (P= 0.05) in the susceptible Imbali cultivar. Zymogram analysis identified lytic bands at ~45kDa (PG gel) and ~30kDa (PME gel). The activity profile of PME was similar for all cultivars. However, PG activity of susceptible Imbali was high in dry seeds and at the R4 and R6 stages of plant development, whereas the more resistant cultivars displayed high activity at the VC stage only. It is concluded that high PG activity at R4 stage is a more reliable determinant of green bean propensity to cotyledonal cracking. / Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2005.

Soil mineral N and soil water content at planting, biomass accumulation, yield and grain quality parameters (hectolitermass and protein percentage) were measured on an unfertilized and recommended-N-application treatment during two consecutive growing seasons (1997-1998). The trials were planted in a fallow-wheat-wheat cropping system at three representative localities in the summer rainfall region of South Africa. High levels of available soil water and mineral N were measured following the fallow period preceding the start of the trials in 1997. For example, soil water content was 81.7%, 69.6%, and 78.2% of DUL at Bethlehem, Kroonstad and Petrusburg respectively. Although comparable total soil profile water contents to 1997 were measured in 1998 at all three sites, the cultivation zone (0-400 mm) had a substantially lower soil water content. This was due to erratic rainfall distribution during the fallow period, which prevented effective soil cultivation management, subsequent soil water conservation and residue decomposition. Undecomposed residue in the cultivation layer at planting appeared to affect availability of soil mineral N to the growing crop. At planting in 1998, undecomposed crop residue amounted to 53.6% at Bethlehem, 32.5% at Kroonstad and 46.9% at Petrusburg of that added at harvest in 1997. Soil mineral N was lower at planting in 1998 compared to 1997 due to decomposing residue (C:N ratio of above 73) in the cultivation zone immobilizing soil mineral N. This reduced initial growth, N accumulation, yield, and grain protein percentage without additional fertilizer N. Distribution of soil mineral N showed notable amounts in the 600-1200 mm soil layers, with limited changes over the trial period. This was linked to low root exploration of these soil layers (10-15% of total root distribution). The ratios of soil mineral NH(4+):N0(3)- for the different soil layers indicated similar values over the trial period. Climatic data for the localities indicated differences in the amount and distribution of rainfall and temperatures during the study period, which influenced crop development, yield and grain protein percentage. At Bethlehem above average in-season rainfall was measured during 1997, at Kroonstad average rainfall and at Petrusburg below average in-season rainfall. Response to applied N at the localities varied in magnitude during 1997. Nitrogen application significantly increased N concentrations of plant components, N uptake, yield and grain protein percentage, although values for all these parameters were lower in 1998 than in 1997. Indeed higher yields were produced in 1997 (mean=1.838 t ha(-1)) compared to 1998 (mean=0.980 t ha(-1)). A significant yield response to applied N was measured at the two higher yielding localities in both cropping years, but there was no significant response at the lower yielding locality. The limiting factors appeared to be the availability of soil water and residual soil mineral N. From the calculated response functions, the variables soil water content at planting, soil mineral N content at planting, in-season rainfall, and added fertilizer N explained the bulk of the variations in grain protein percentage, plant N uptake, and yields. It was concluded that the present fertilizer N recommendation system for dryland wheat production, which is based on fertilizer response curves for specific yield potentials, should be augmented by using initial soil mineral N and water contents in the profile measured prior to planting. / Thesis (M.Sc.Agric.)-University of Natal, Pietermaritzburg, 2002.

Flooding cycle in wetland rice (Oryza sativa L.) production systems is often subject to seasonal and cultural variations which may affect the availability and uptake of nitrogen in different ways. These factors may more or less influence the physiological and growth responses of the plant. In an effort to improve productivity in rice cropping systems, two controlled environment studies and a field trial were conducted to evaluate the growth and yield responses of rice to different flooding regimes and nitrogen fertilizer management strategies. In the first glasshouse trial, an upland cultivar (GM-1) was used to study the effects of four flooding regimes and three nitrogen application rates on the tillering, yield components and grain yield of rice. The field study determined the applicability of the results of the glasshouse trial to out-door environmental conditions, with the aim of gaining further insight into the impact of nitrogen application strategy on tiller and grain qualities. Two wetland cultivars (FKR-19 and N-19) and GM-1 were used to evaluate the effects of two flooding regimes and two nitrogen topdressing patterns. The second glasshouse trial determined the effect of hydro-priming on the establishment of direct seeded rice, and the effect of flooding on aerenchyma formation in rice roots. Results of the studies showed that flooding with standing water of 5 cm above the soil surface, irrespective of when it occurred, and nitrogen application increased the number of tillers and panicles, above ground dry matter, nitrogen uptake and grain yield of rice. However, late flooding and high nitrogen application rate of 220 kg ha-1 were found to encourage the production of late tillers, thereby reducing the efficiency of nitrogen use for grain production. Nitrogen application in three split doses tended to increase plant nitrogen content at heading; it slightly increased the protein content of the grains at maturity, but reduced the amylose content of the starch granules. Nitrogen application in two split doses led to increased grain yield in non-flooded plants, while the three-split treatment increased nitrogen uptake and grain yield in the flooded plants. Flooding significantly increased aerenchyma formation in the cortical tissues of rice roots, particularly at 50 mm behind the root tips. Hydro-priming seeds for 48 h improved plant establishment by shortening the germination and emergence times, and increasing the height and dry matter accumulation of seedlings, thereby ameliorating the susceptibility of rice to flooding stress. Overall, the thesis affirmed that controlled flooding is beneficial to rice production since it enhanced the growth and yield of the plant. It further revealed that early flooding and appropriate timing of moderate nitrogen application can ensure the conservation of water and nitrogen resources, including the quality of the environment, with no significant consequence for yield and productivity of the crop. / Thesis (M.Sc.Agric.)-University of KwaZulu-Natal, Pietermaritzburg, 2010.

Crop rotation is not commonly practised in the sugarcane industry in South Africa. It has, however, proven to be beneficial to other crops in South Africa. The objective of this study was to determine the impact of soybean-sugarcane crop rotation on selected physiological and phenological indicators of sugarcane performance and its subsequent effect on cane and estimated recoverable crystal (ERC) yields. A field trial was conducted at Mount Edgecombe, where soybean cultivar A5409RG and sugarcane cultivar NCo376 were planted under drip irrigation with different management practices. After the soybean crop, the following sugarcane crop was planted and fertilized with different levels of nitrogen (N) fertilizer (50% and 100% of the recommended N rate). The effects on sugarcane growth were recorded by taking into consideration date of emergence, plant height, tiller population, leaf N, plant performance index and chlorophyll content. Sugarcane yield and quality at harvest were also evaluated. Tiller population in all crop rotation treatments at Mount Edgecombe weresignificantly (P<0.05) higher than the monocrop treatment. There was a trend of increased leaf N in all of the cane-after-soya (crop rotation) crops compared to the cane-after-cane (monocrop) treatment, although this was not significant. A similar pattern was obtained with respect to the chlorophyll content and plant performance index. Sugarcane yields at Mount Edgecombe did not differ significantly between monocrop and crop rotation treatments. Crop rotation with soybean is beneficial for cane production, but its long term impact on soil quality and farm economy requires further investigation. / M.Sc.Agric. University of KwaZulu-Natal, Pietermaritzburg 2013.

The wild watermelon, Citrullus lanatus L. was among the most important foodstuffs to a number of African communities, until the colonists introduced their own foodstuffs in a process that was highly supported by the laws of the time. However, there is now a growing realization by government and other stakeholders of the importance of indigenous crops (including the wild watermelon) as substitute food stuff to improve food security. Wild watermelon is an adaptable crop, which can contribute to food security as it has a potential for commercialization. However, there are no records on the production of wild watermelon with reference to optimum planting density and the effects of mulch on the growth and development of the crop. To investigate this issue, which the smallholder farmers are faced with, a study that designed to (a) determine the effects of population density on growth and yield of wild watermelon and (b) investigate the effects of mulching on growth and yield of wild watermelon under field conditions. The study was undertaken over two seasons during which two different types of propagules, namely seed and seedlings, were used. A field study of wild watermelon establishment and yield using seeds and seedlings to compare the effects of different population densities (3000, 6000, 9000 and 12000 plants/ha) and mulching rates (0, 2.5 and 5 t/ha) based on the availability of grass on soil water, temperature, vine length (height), number of branches and leaves per plant, fruit number, total yields, fruit size and weed distribution was conducted at Dohne Agricultural Development Institute (Lat-32.52521; Long – 27.46119, alt. 907 m above sea-level) over a two year period ( 2009 – 2011 growing season). Results on data collection and analysis of growth and yield parameters are that: When seed was used as means of propagation, there were significant effects (p 0.05) of mulching and population density, on soil temperature and volumetric water content. However, no significant differences were found with regards to vine length, number of branches and leaves per plant. Concerning yield, there were no significant differences recorded on any of the measured parameters in response to mulch. Yet, with population density, significant differences were noted on fruit number per hectare and total yield at p 0.05. The number of fruits and total yield per hectare increased as plant population increased, resulting in high yields to range from 9000 -12 000 plants per hectare with both seed and seedling propagules used during the study period with or without mulching. Seedling propagules were associated with differences in soil temperature and volumetric water content with regards to mulching and population density (p 0.05). Results obtained from this study in both years, revealed that yield is more influenced by plant population density than by mulching. Mulching has been found to be ineffective as far as growth and yield are concerned, but it was found to influence soil temperature and volumetric water content. / Thesis (M.Sc.Agric.)-University of KwaZulu-Natal, Pietermaritzburg, 2012.

Cowpea (Vigna unguiculata (L.) Walp) is an important protein-rich grain legume of major economic importance. It is widely grown by small-scale farmers in the arid and semi-arid regions of the world where it is cultivated for its leaves, fresh immature pods and dry grains. However, it is also an underutilized grain legume. In sub-Saharan Africa where most of the cowpea is produced, drought stress is one of the major factors limiting its productivity. Despite the inherent capacity to survive drought stress, several cowpea varieties are affected by mid and late season drought. Therefore, varieties with a higher tolerance to drought stress are required to obtain higher and more stable yields. The objectives of this study were: (i) to determine morphological responses of four dual-purpose cowpea landraces to water deficits during vegetative and reproductive stages (ii) to determine physiological responses of four dual-purpose cowpea landraces to water deficits and recovery during the reproductive stage (iii) to determine yield performance of cowpea landraces after recovery from water stress and how this relates to (ii) above. Four cowpea landraces namely; Lebudu, Lehlodi, Sejwaleng and Morathathane collectedfrom Kgohloane and Ga-Mphela villages, Limpopo Province, South Africa were used in the study. Pot experiments were conducted under glasshouse conditions at the Controlled Environment Facility (CEF), University of KwaZulu-Natal. The first pot experiment evaluated the morphological responses of four cowpea landraces to water stress and recovery. The study was conducted as a single factor experiment laid out in randomized complete block design (RCBD). The treatments (four cowpea landraces) were each planted in 40 pots giving a total of 160 experimental units (drained polyethylene pots with a 5 litre capacity). Each plant in each pot was treated as a replicate. Plants were well-watered until the formation of six fully expanded trifoliates, then irrigation was withheld for 28 days to simulate drought stress during the vegetative growth. The imposition of drought stress was terminated by re-watering all plants after 28 days. The cowpea plants were re-watered sufficiently and allowed to grow until the four landraces reached 50% flowering stage. Watering was withheld again at 50% flowering for a two-week period for all the four landraces to simulate drought stress during the reproductive growth. The second experiment was conducted to investigate physiological responses of the four cowpea landraces to water stress during the reproductive stage. The experiment was laid out as a 4 x 2 factorial treatment structure in randomized complete design (CRD) with the following three factors: cowpea landraces – 4 levels (Lebudu, Lehlodi, Sejwaleng and Morathathane), water regimes – 2 levels (stressed and well-watered) treatment combinations each replicated 20 times (20 pots each containing one plant) giving a total of 160 experimental units (drained polyethylene pots with a 5 litre capacity). Data on morphological responses were collected and included: number of green vs. senesced leaves, visual assessment of leaf greenness, stem, branch greenness and survival percentage. Physiological responses to water stress were determined during the reproductive stage and included: leaf water potential, relative water content, stomatal conductance, proline content, chlorophyll content, carotenoid content, chlorophyll a content, phenolics (free and membrane-bound), total antioxidant capacity and chlorophyll fluorescence parameters (Fv/Fm). Genstat 14th edition (VSN International, UK) was used to perform analyses of variance (ANOVA) and differences between means were determined by the Least Significant Differences (LSD) at the 5% probability level. Landraces showed different morphological responses during both vegetative and reproductive growth stages. Lebudu, Lehlodi and Sejwaleng displayed a strong ability to maintain stem greenness longer as compared to Morathathane during vegetative growth. Lebudu delayed leaf senescence more than other landraces; no differences in survival were observed. All landraces survived for 28 days without water and resumed growth after re-watering. During the reproductive stage, Lebudu displayed a strong ability to maintain leaf, branches and stem greenness longer and showed relatively higher tolerance to drought stress compared to other three landraces. Water stress caused a decline in leaf water potential, relative water content, carotenoid content, chlorophyll content, stomatal conductance and increased proline content, phenolics, chlorophyll a content, total antioxidant capacity and while chlorophyll fluorescence parameter, Fv/Fm, was not affected. All landraces maintained higher relative water content above a critical threshold with Sejwaleng maintaining a significantly higher RWC (69%) than Lehlodi, Lebudu and Morathathane. Morathathane developed a more negative leaf water potential at maximum stress than Lebudu, Lehlodi and Sejwaleng. Stomatal closure was observed in all cowpea landraces during water stress, but re-opened after re-watering. Chlorophyll content was considerably reduced in Morathathane as compared to Lebudu, Lehlodi and Sejwaleng. No significant differences were observed between the cowpea landraces with respect to carotenoid content at maximum stress. Chlorophyll a content increased significantly for Morathathane as compared to Lebudu, Lehlodi and Sejwaleng. High accumulation of proline was observed for Lebudu, Lehlodi and Morathathane as compared to Sejwaleng, which showed a very slow accumulation of proline. Lebudu, Lehlodi and Sejwaleng showed an increase in phenolic compounds while a decline was observed for Morathathane. Total antioxidant capacity (TAOC) was high in all cowpea landraces during water stress. Also, all chlorophyll fluorescence parameters showed that cowpea landraces had efficient photo-protection mechanisms during drought stress. After re-watering, relative water content, leaf water potential, stomatal conductance, chlorophyll content, carotenoids, chlorophyll a, proline content and TAOC recovered and reached the same level as that of well-watered plants. All four landraces were re-watered after the imposition of stress and above ground biomass, pod mass and number and seed yield determined. Although there was a reduction in the total above-ground biomass, pod mass and number in all four landraces under water stress compared to the well–watered treatment; this was not statistically significant (P > 0.05). Furthermore, no significant differences (P > 0.05) were observed between the four landraces with respect to seed yield under stressed and well-watered conditions. This study established that cowpea landraces vary with respect to the various morphological and physiological adaptive mechanisms in response to water deficits. Such adaptive mechanisms probably ensure their survival under severe water stress conditions until the next rainfall and therefore allowing them to produce reasonably relatively higher leaf and seed yield. Detailed knowledge of these mechanisms in the landraces could be useful in the genetic enhancement and breeding for drought tolerance in the existing cowpea germplasm. / Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2013.

An experiment was conducted at Pongola (27°24´S, 31°25´E; 308m altitude) in South Africa to study the effects of season on growth and potential biomass and sucrose yields on nine commercial sugarcane cultivars. The treatments that were the focus in this study consisted of the cultivars NCo376, N25 and N26 ratooned in March, April, May, August and December. The crops were well fertilized and kept free of weeds and diseases. Irrigation applications were scheduled with a computer programme to keep the crops free of stress at all times. Shoot populations were counted regularly to study shoot density dynamics. Leaf appearance rates, sizes, numbers and senescence were measured to study the development of green leaf area. Green foliage, dead trash and stalk mass were measured at 4, 8, 10, 11 and 12 months in each of the starting times and also at 13 months in the March, April and May ratoon crops. The fibre, sucrose and non-sucrose content of stalks were determined on these harvesting occasions. Yields were calculated in terms of individual shoots and area (m‾²). The fraction of PAR intercepted by the developing canopies was measured until full canopy and daily intercepted solar radiation was interpolated for the entire crop. An automated meteorological station adjacent to the experiment site provided daily weather data. Shoot densities were described by thermal time, however, average peak shoot densities were lowest in the May ratoon (31.8 m‾²) and highest in the December ratoon (48.7 m‾²). Shoot senescence was most rapid in August and December ratoons. At the final harvest shoot densities were highest in the March, April and May ratoon (14.8 to 14.2 m‾²) crops. NCo376 (16.4 m‾²) and N25 (13.6 m‾²) had higher final shoot densities than N26 (10.5 m‾²). Leaf appearance rate was also well described by thermal time, however the first twelve leaves took longer to appear in crops started in December i.e. the first phyllochron was longer (109.5°C d) than in crops started at other times (80.4 to 94.5°C d). Leaves produced during the early stages of December and August ratoon crops were larger (e.g leaf number 13 of N26 was 443 to 378 cm²) than in other crops. April and May ratoon crops produced much smaller leaves (e.g leaf number 9 of N26 was 170 to 105 cm²). Leaf senescence was slower in April and May ratoon crops (0.36 to 0.46 leaves per 100°C d) than in March (0.51 to 0.59 leaves per 100°C d) or August and December ratoon crops (0.60 to 0.68 leaves per 100°C d). December ratoon crops produced very high green leaf area indexes (LAI) (>7.0) at the age of four months; all other crops had lower LAI (3.3 to 6.0) and most peaked later (8 to 11 months of age). The LAI of N25 peaked at the age of 8 months while NCo376 and N26 peaked when 10 to 11 months old. Seasonal fraction of solar radiation intercepted was high in the March ratoon crops (0.84) and declined to 0.63 in the May ratoon crops and was highest in the December ratoon crop (0.88). N26 intercepted lower fractions of PAR than NCo376 and N25, particularly in the May and August ratoon crops. Biomass accumulation, although initially slow, tended to be linear in the March, April and May ratoon crops in relation to intercepted radiation. In August and particularly in the December ratoons biomass accumulation was initially rapid, and RUEs were high (2.65 g MJ‾¹ at 114 days in the December ratoon crops). However, biomass accumulation slowed when these December ratoon crops experienced winter. Low growth rates after winter, as well as low shoot densities resulted in December ratoon crops having produced significantly lower above-ground biomass yields (4 886 g m‾² at the age of 12 months) than March, April and May ratoon crops (6 760 to 5 715 gm‾² at the age of 12 months). The December ratoon crops responded poorly to the better growing conditions in spring and second summer and accumulated little biomass after winter. N26 shoots grew rapidly during the first 6-8 months of the December ratoon crop and it yielded better than NCo376 and N25 at harvesting (biomass yields were 5.8 and 13.3% higher at the age of 12 months, respectively). April ratoons produced significantly higher biomass yields (6 760 g m‾²) than March, August and December ratoons. May ratoon crops produced the highest cane fresh mass yields (18 151 g m‾²) and April, May and August ratoons produced significantly higher sucrose yields than March and December ratoons. The highest sucrose yield was produced by the April ratoon crop of N26 (2 385 g m‾²). On average, across the five ratoon dates, NCo376, N25 and N26 produced similar sucrose yields (1 902 to 1 959 g m‾²). Foliage production was severely limited during winter while sucrose accumulation was less affected by the low temperatures, resulting in accumulation of sucrose in the top sections of the culm. Low temperatures slowed the development of canopies in March, April and May ratoon crops, but these crops were able to recover their growth rates and produced high biomass and sucrose yields at the age of 12 months. The December ratoons experienced low winter temperatures (<12°C) when they had already accumulated relatively high yields and became moribund during winter. They were unable to accumulate any significant amounts of biomass during final four months before the final harvest at the age of 12 months. NCo376, N25 and N26 all yielded poorly in the December ratoon crop. However, there are cultivars that appear to be less sensitive to the low winters and are able to yield relatively well when they are ratooned in December. Sucrose yields of March, April and May ratoons were increased substantially (10.6 to 22.7%) by harvesting at the age of 13 months rather than at the age of 12 months. The poor growth of December ratoon crops after winter is possibly due to the recently revealed feedback signaling by high sugar levels induced by low temperatures on photosynthesis. The incorporation of the effects of low temperature and the feedback signaling with the objective of better simulating yields of December ratoons is a proposed study at the South African Sugarcane Research Institute. Annual mean sucrose yields of NCo376, N25 and N26 crops were estimated to be 17% higher in March than in December ratoons. The suggested short term remedy therefore of the poor December yields is to shift milling seasons to include March and exclude December harvested crops in the northern irrigated regions. March crops grow vigorously during the months close to harvesting and therefore have lower levels of sucrose content which can be corrected with chemical ripeners. / Thesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2009.

Despite the importance of taro (Colocasia esculenta L. Schott) as a food security crop, scientific research on it is scanty in South Africa. Production site, planting date and fertiliser regime affect crop performance and quality, particularly that of cultivars, because they tend to be adapted to specific localities. Storage temperature and packaging method on the other hand affect the shelf-life. To investigate performance and quality of three taro cultivars in response to planting date and fertilisation, a study was carried out at two sites in KwaZulu-Natal, South Africa (Ukulinga and Umbumbulu), during the 2007/2008 growing seasons. The effect of two storage temperatures (12oC and ambient temperature) and three packaging methods (polyethylene bags, mesh bags and open boxes) on cormel quality following storage was also investigated for three cultivars. Delayed planting negatively affected the number of cormels plant-1 and fresh cormel mass plant-1. Fertilisation and cultivar affected the number of cormels plant-1 and fresh cormel mass plant-1 only when planting was done in October and November at both sites. Fertilisation increased the number of cormels plant-1 for all cultivars except Dumbe-dumbe. Dumbe-dumbe had the lowest number of cormels plant-1 but the highest number of marketable cormels plant-1. Dumbe-dumbe showed the lowest fresh cormel mass plant-1 in October and the highest in November at Ukulinga. Fertisation increased fresh cormel mass plant-1 in October at Umbumbulu. Dry matter content was negatively affected by fertilisation at Ukulinga. The response of dry matter content, specific gravity, protein, minerals, reducing sugars and starch content was variable depending on cultivar. Delayed planting negatively affected starch content for Dumbe-dumbe and Pitshi at Ukulinga. Fertilisation decreased starch content of Pitshi, while delayed planting increased sugar content for Dumbe-dumbe and decreased it for Mgingqeni and Pitshi at Umbumbulu. Dumbe-dumbe had higher starch content and higher reducing sugars. Considering all growth and quality parameters, it is recommended that Dumbe-dumbe is the best taro cultivar for crisping and the best time to plant it is October with 160 kg N ha-1 of organic fertiliser and November with 320 kg N ha-1 at Ukulinga whereas at Umbumbulu the best time to plant Dumbe-dumbe is October with 320 kg N ha-1 of the fertiliser. Starch granules degradation, alpha-amylase activity and sprouting increased with storage time and storage temperature. Cormels of Mgingqeni stored in polyethylene bags showed highest alpha-amylase activity and sprouting. Reducing sugar content increased and starch content decreased with time in storage and decline in storage temperature. It is recommended that taro cormels be stored in mesh bags at 12oC. The chapters of this thesis represent different studies presented as different papers. Chapter 1 is a general introduction to explain the study background and hypothesis. Chapter 2 is a general review of literature. Chapter 3 is on growth, development and yield of taro in response to planting date and fertilisation. Chapter 4 is on the influence of planting date and organic fertiliser on crisping quality of taro cormels. Chapter 5 is on changes in the surface morphology of starch granules and alpha-amylase activity of taro during storage. Chapter 6 is on the effects of pre- and post-harvest practices on starch and reducing sugars of taro. The last chapter is a general discussion and conclusions. / Thesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2009.

Cowpea (Vigna unguiculata. L) is an important African crop. However, it is also an underutilized grain legume. Consequently, there is not enough research data on cowpea seed physiology. Whereas there is evidence of cowpea being a drought tolerant crop, there is no evidence to associate plant drought tolerance with seed quality in response to water stress. This study sought to understand the effect of production site and water stress on cowpea seed quality development with respect to germination capacity and vigour. Patterns of raffinose family of oligosaccharides (RFO) during seed development to mature dry stage were used to physiologically relate seed performance to water stress. The effect of water stress and exogenous ABA on the accumulation of stress LEA proteins (dehydrins) in relation to seed quality development and germination was investigated. RFOs are known for their roles in desiccation sensitivity but no studies have shown their significance in cowpeas. Seeds of six cowpea cultivars were produced at two distinct growth sites characterised by irrigated and dry land conditions. The seeds were assessed during six developmental stages, for water content, dry matter accumulation, and performance. Harvested seeds were then planted in a pot experiment under controlled conditions to examine the effect of water stress on seed quality development and data collected during three developmental stages. Harvested seeds from the pot experiment were subsequently analyzed for changes in RFO accumulation during development using gas chromatography. The seeds were also used to investigate the effect of water stress and ABA on the accumulation of stress LEA proteins (dehydrins) in relation to seed quality development in cowpea. In addition, this study evaluated the use of image analysis as a method that can be used to objectively determine seed coat colour variation in cowpea. Statistical variation in individual seed’s solute leakage for cowpea cultivars differing in seed coat colour and produced under different environmental conditions was explored and correlations were done between seed conductivity test with other aspects of seed performance during germination. Furthermore the results of the conductivity test were compared with accelerated aging test, in relation to seed performance. The study provided evidence that cowpea seed lots produced under different environmental, and possibly management conditions may not differ with respect to seed quality as determined by germination capacity and vigour. However, significant differences between sites with respect to seed maturation patterns determined by water content and dry matter accumulation were observed. Adverse maternal environmental effects on the subsequent performance of seeds in a drought tolerant crop may not necessarily lead to poor performance. Cultivar differences in response to simulated drought conditions at the whole plant and tissue level can be considerable and highly variable; however, these differences may not have adverse effects on the germination and vigour of the seeds. Drought avoidance mechanisms at the whole plant level in cowpea are quite efficient in allowing the species to adapt to simulated drought conditions. These mechanisms may allow the cowpea cultivars to maintain metabolism and restore conditions for their continued growth under water stress; and produce few seeds of high germination capacity and vigour. Stachyose was found to be the predominant member of the raffinose family of oligosaccharides in cowpea. It is suggested that stachyose accumulation could be used as an indicator of stress tolerance in cowpea. However, the relationship between RFO concentration and the acquisition of desiccation remained as a matter of speculation in the present study and is still generally inconclusive. There was no evidence to suggest the acquisition of maximum desiccation tolerance is associated with maximum seed vigour. It is suggested in cowpea, which is drought tolerant, that maximum vigour does not necessarily imply the acquisition of maximum desiccation tolerance; rather there is a minimum level of desiccation tolerance that is required for the development of optimal seed vigour. The use of an in vivo approach in the study of LEA function in cowpea enabled the accurate comparison of two different groups of LEA proteins in developing cowpea seeds under conditions of water stress and in relation to germination and vigour. Both group 1 LEA and group 2 LEA (dehydrin) were shown to increase in concentration in response to water stress. In addition group 1 LEA protein was observed to be relatively abundant in cowpea seeds. A maternal influence on LEA protein gene expression under conditions of water stress, which may induce dehydrin accumulation vii during the earlier stages of seed development, was implied by the observation that dehydrin-like proteins were induced after two weeks of development in cowpea plants subjected to stress during the vegetative phase. In addition, the exogenous application of ABA delayed radicle protrusion; this was associated with a delay in the disappearance of LEA proteins and is suggestive of a relationship between LEA protein accumulation and the acquisition of desiccation tolerance. The study has demonstrated that image analysis can objectively discriminate seed coat colour variation in cowpea. Dark coloured seeds in general performed better than light coloured seeds; however seed coat colour was not always associated with better performance. A newly developed Aging Stress Differential Index (ASDI) has been used in this study to demonstrate a link between seed coat colour and sensitivity to water stress. The ASDI correlated well with the observations relating stress tolerance to stachyose accumulation. The skewed distribution patterns in individual electrical conductivity and the presence of extreme values may have implications with respect to the suitability of using standard statistical analyses which compare mean values to evaluate such data. In addition variation in individual electrical conductivity may also be influenced by cultivar differences and the chemical composition of the seed coat. Therefore associations between seed coat colour and electrical conductivity as a measure of performance should be treated with caution. The AA test does reflect changes in seed vigour, however ranked electrical conductivity values after AA did not consistently reflect differences in seed performance between cultivars and sites, and they did not correlate well with other aspects of performance. / Thesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2007.

The aim of the study was to determine the effect of day/night temperatures (22/l6°C, 2712l oC and 33/27°C) and phosphorus levels (0, 0.12 and 1.2g per 20 kg soil) on seedling establishment and seed viability during three stages of seed development (15, 22 and 33 days after flowering) for seed of normal and quality protein maize cultivars. Soluble carbohydrate accumulation and mineral element content were determined using environmental scanning electron microscopy (ESEM) in relation to seed phytate levels and seed germination capacity at different stages of development. Leaf emergence rate and plant height during seed development were significantly (P < 0.05) influenced by temperature and phosphorus nutrition. Phosphorus in seed is stored primarily in the form of phytic acid, also known as phytate. Accumulation of phytate takes place during maturation phase of seed development. Phosphorus nutrition and temperature also caused a.significant (P < 0.05) increase in seed germination at all stages of seed development. Furthermore, phosphorus nutrition and temperature influenced occurrence of soluble carbohydrates in seeds. Myo-inositol, the sugar alcohol that forms the basic structure of phytate, was increased by P nutrition and increasing growth temperature. Whereas, QPM maize was generally found to perform poorly than normal maize, with respect to phytate content, seed germination and seedling establishment, both cultivars displayed the same responses to phosphorus nutrition and temperature. In both cultivars, globoids, the sites of phytate synthesis and storage, were found only in the embryonic axis. Subsequently, there were significantly low levels of mineral elements (P, Mg and K) found in the endosperm, compared with embryonic axis. This finding suggested that the embryonic axis plays a major role in seed performance, through its effects emanating from phyate metabolism. Myo-inositol plays a role in membrane biogenesis during stress conditions such as temperature by maintaining the integrity of the cell wall and minimizes the leaching of cations essential during germination. Myo-inositol, although it occurs in small concentrations, could be used to indicate seed quality in maize, because its accumulation was found to be associated with enhanced phyate levels and better seed germination in a wide range of temperatures. Low vigour seeds are associated with high electrolyte leakage during imbibition. Mineral elements form a significant portion of the imbibition leachate, which causes seeds to lose nutrients for early seedling growth. This study provided evidence that phosphorus nutrition can alleviate poor seed vigour of maize by improving phytate levels. / Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2006.