PhD (Psychology) / Department of Psychology

MSc (Botany) / Department of Botany / See the attached abstract below

Bibliography: leave 85-90. / Health care systems play an important role in maintaining good health in communities. In South Africa, Africans are continually faced with the dilemma of choosing western or traditional values. The literature has shown that African people use both western and traditional systems simultaneously. The South African government is also considering to include traditional healers in the national health policy .This study examines the opinions of African parents or caretakers of children at Red Cross War Memorial Children' s Hospital regarding their use of western and traditional health systems and their opinion regarding their linkage. The research method for this exploratory study was a focus group and structured interview. The results indicated that in the communities from which the respondents were drawn there are many Africans who consult traditional healers. The results further indicated that many Africans consult both western doctors and traditional healers for the same medical problem. The results also indicated that the respondents considered it necessary to link traditional healers to western health settings. Recommendations for future research are included.

Thesis (M. Sc. (Microbiology)) -- University of Limpopo, 2022 / Sexually transmitted infections represent one of the main health problems related to reproductive and sexual function, constituting one of the main causes of infertility. Plants have been employed traditionally in treating diseases for centuries. Elephantorrhiza burkei, Securidaca longepedunculata and Wrightia natalensis medicinal plants were selected for this study based on their use in traditional medicine to treat sexual health problems in Limpopo Province. The aim of the study was to determine antimicrobial and antioxidant properties of the selected plants. Different extraction procedures coupled with solvents of varying polarities were used in the extraction of the plant materials. Possible microbial contaminants were identified using the VITEK 2 instrument. Qualitative phytochemical analysis was determined using standard chemical tests and Thin-Layer Chromatography. Total polyphenol content was quantified calorimetrically. Antioxidant activity was quantified using 2, 2-diphenyl- 1-picrylhydrazyl (DPPH) and ferric reducing power assays. In vitro antimicrobial activities were determined using a broth micro-dilution assay and bioautography. Combinational effects of the addition of multiple plant species on antimicrobial activity were investigated for additive, synergistic and antagonistic interactions. The plant species phytochemical profile contained polyphenols that are known to have antimicrobial effects against two Gram-positive bacteria, Staphylococcus aureus (ATCC 29213) and Enterococcus faecalis (ATCC 29212), a Gram-negative bacterium, as well as the fungus, Candida albicans (ATCC 10231). Elephantorrhiza burkei exhibited high antioxidant activity and antimicrobial activity against the test pathogens, although most of the interactions were antagonistic and indifferent. Toxicity was found in the herbal treatment and acute toxicity was found in the selected plants. Based on the good correlations which were found in E. burkei, it was selected for bioactivity guided by an isolation of antibacterial compounds. Elephantorrhiza burkei was not successfully isolated, but the fraction collected showed exceptional biological activity that validates its usage against disease-causing pathogens related to sexually transmitted infections, which compromises male sexual health. This study suggests that the evaluated plants are potential sources of novel anti-infective agents. Further in vivo and in vitro studies are recommended for all the plants, respectively. / University of Limpopo and CSIR

Thesis (M. A. (Psychology)) -- University of Limpopo, 2020 / Though recent South African legislation and policy documents have called for closer collaboration between Western-trained and traditional health practitioners, there is little evidence to show that there is a formal collaboration between the two categories of health care providers. Located within the interpretivist paradigm, and using an exploratory descriptive design, the researcher sought to explore the perceptions of Western-trained health practitioners (WTHPs) in Sekhukhune District (Limpopo Province) towards collaboration between themselves and traditional health practitioners (THPs) in treating mental illness. Seventeen WTHPs (males = 07; females = 10) from three hospitals in Sekhukhune District were selected through purposive sampling and requested to take part in the study. The sample comprised of five clinical psychologists, five medical officers working in psychiatric units, and seven psychiatric nurses. Data were collected using semi-structured interviews and analysed through thematic analysis. Specifically, Renata Tesch’s eight steps were used to analyse the data. The following psychological themes emerged from the study: (a) shared goals on collaboration; (b) a good effect on collaboration is anticipated; (c) managing interdependence between traditional and Western-trained practitioners; (d) proposed ideal structures of governance to govern the collaboration; (e) recommended legislations and policies on collaboration; (f) suggested factors that may foster collaboration; (g) proposed factors that hinder collaboration; and, (h) referral systems that exist in the health care. The findings suggest that some WTHPs are willing to collaborate with THPs, especially if proper guidelines for collaboration could be provided by the government. Some recommendations on an ideal structure of governance and legislation on collaboration were made by the WTHPs. The WTHPs highlighted factors that may hinder or facilitate closer collaboration between themselves and THPs in providing mental health services to communities.

Thesis (M. Sc. (Biochemistry)) --University of Limpopo, 2017 / Diabetes mellitus is a chronic metabolic disorder characterised by perpetual hyperglycaemia. Various oral pharmacological theraputic management strategies currently exist but are too expensive and having a host of undesirable side effects. Therefore people resort to the use of traditional medicinal plants as they offer a cost effective and readily available health care avenue. Despite the wide-spread use of traditional medicinal plants, several worrisome concerns about their effectiveness, clinical modes of action and safety have been raised. Leaves of five selected plants (Toona celliata, Seriphium plumosum, Schkuhria pinnata, Olea africana, Opuntia ficus-indica) were collected from Mankweng area, Capricon Local Municipality, Limpopo province, South Africa. Ground plant materials were exhaustively extracted by maceration in methanol, acetone or hexane. The presence of different plant secondary metabolites in the crude extracts was determined using various standard chemical tests and thin layer chromatography (TLC). A myriad of compounds which represented various secondary plant metabolites groups were observed on the TLC plates and were best resolved in the non-polar (BEA) and intermediate (CEF) mobile phases. The total phenolic content and total flavonoids of the different extracts were determined spectrophotometrically using the Folin-Ciocalteu`s phenol reagent method and Aluminium chloride colorimetric assay respectively. The plants contained comparatively higher amounts of total phenolic compounds as compared to the flavonoids. The antiglycation activity of the plant extracts were determined using the bovine serum albumin assay. The acetone extract of Seriphium plumosum (SPlA) exhibited the most glycation inhibitory activity among all the examined extracts, as it resulted in 2,22% glycation. The antioxidant potential of each of the different extracts was quantitatively determined spectrophotometrically using the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay and the ferric ion reducing power assay. The methanol extract of Seriphium plumosum showed the best antioxidant activity among all the extracts in this study. It exhibited the lowest EC50 values of 0.72 mg/ml and 2.31 mg/ml for the DPPH scavenging activity and the ferric reducing power assay respectively. The cytotoxicity profiles of the different plant extracts on C2C12 cell line were determined using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium xiii bromide (MTT) assay. It was concluded that since the all the extracts investigated had CC50 values greater than 50 μg/ml they were generally non-toxic. The amount of glucose taken up by differentiated C2C12 cells was quantified using the glucose uptake assay. Treatment of the C2C12 cells with the hexane extract of Seriphium plumosum resulted in the best glucose utilisation effect of 35,77% which was higher than that of insulin which was 26,06% after 6 hours. The translocation assay was used to determine the effect of the plant extract on GLUT4 translocation while the expression of various mitogen activated protein kinases in the cells was determined using the human MAPK profiler assay. It was established that treatment with Seriphium plumosum hexane extract resulted in increased GLUT4 translocation from the intracellular vesicular stores to the cell surface membrane. The increase in GLUT4 translocation may have resulted from the upregulation of expression of phosphorylated Akt-1, Akt-2, GSK3β, ERK1, ERK2 p70S kinase and MKK3 under the influence of Seriphium plumosum hexane extract. The study documents a probable insulin-mimetic activity of the hexane extract of Seriphium plumosum. This activity may be responsible for its hypoglycaemic capability and may occur via the augmentation of proximal mitogen activated protein kinases involved in the GLUT4 translocation pathway. Further investigations need to be conducted to ascertain this novel finding which may help provide a cost-effective and readily available antidiabetic therapeutic agent. / National Research Foundation (NRF)

Thesis (Ph.D. (Psychology)) -- University of Limpopo, 2023 / With the realisation that both the traditional and western health care systems are consulted in South Africa, the Traditional Health Practitioners Act, No 22 of 2007 was promulgated. In addition to the Act, there was a growing body of literature proposing the integration of the two health systems to improve the overall South African health system. This study explored the possibility for such collaboration by investigating case formulation by western-trained clinical psychologists and traditional health practitioners regarding selected cases of mental disorders. A qualitative research design, specifically a case study method was used in the present study. Six clinical psychologists and six traditional health practitioners were selected through purposive sampling and were requested to participate in the study. The researcher further sampled two vignettes of Major Depressive Disorder and Schizophrenia for data collection purposes. Data was collected through semi-structured interviews and vignettes and analysed through Braun and Clarke‟s thematic content analysis steps. Six main themes that related to case formulations by clinical psychologists emerged. These themes were: a). Collateral information as a major determinant in the assessment and treatment of mental illness; b). Classifications of mental illness; c). The symptomatology of mental illness; d). Causes of mental illness; e). The multidisciplinary approach in the treatment of mental illness; and f). Views regarding the collaboration of Clinicians and Traditional Health Practitioners. On the other hand, eight themes that related to the case formulations by traditional health practitioners were identified. These themes were: a) Divination as the main process of enquiry, b) Descriptive names of mental illnesses, c) Conceptualisations of stress related and depressive disorders by Traditional Health Practitioners, d) Views on causes of mental illness, e) The effectiveness of the Western approach in the treatment of some forms of mental illness, f) The relationship between religion and African traditional practices; g) A calling as symbolised by symptoms of mental illness, and, h) Traditional health practitioners‟ views on the collaboration between themselves and western health practitioners. The study has further revealed that there are some similarities as well as differences in the way clinical psychologists and traditional health practitioners formulate cases. The convergences in their formulations were revealed in the following themes; a). The conceptualisation of stress and related conditions; b). The western system is the most appropriate in the management of stress-related and depressive disorders and c). The benefits of the collaboration between western and African healing systems. The following divergences were further identified from the way clinical psychologists and traditional health practitioners formulate their cases: a). The conceptualisation of the presenting symptoms from the two cases; b). The causal factors of mental health conditions; c). The initial methods of enquiry; and d). The management of mental health conditions. The study has further revealed that both traditional health practitioners and clinical psychologists have positive views regarding the collaboration of western and African traditional health systems, especially in the management of mental disorders. The study has further uncovered the convergences and divergences in the conceptualisation of mental health conditions between traditional health practitioners and clinical psychologists.

Traditional medicines prepared from Sutherlandia frutescens are used to manage diseases including HIV and cancer. This study aimed at isolating and identifying biologically active compounds isolated from S. frutescens. Sutherlandia frutescens plants were collected in Petrusburg and Paarl. Powdered plant material was extracted using ethanol or water and their metabolite composition was compared using UPLC-MS. A novel cycloartane, an acetylated variant of this compound as well as a Sutherlandioside B triterpenoid was isolated and characterised using chromatographic and analytical techniques such as NMR and UPLC-MS. Preliminary biological studies were conducted to assess the activity of plant extracts on cell toxicity, herpes virus replication and cytokine expression. The results of this study suggest that aqueous extracts from S. frutescens do not appear to be cytotoxic or show anti-herpetic activity, but may activate the immune system by increasing expression of IL-6, IL-10 and TNFα. Further research should be conducted to confirm and optimise these results. / Life and Consumer Sciences / M. Sc. (Life Science)

Thesis (M. Tech. (Biomedical Technology)) -- Central University of Technology, Free State, 2014. / Traditional medicine has been known by mankind since ancient times as a healthcare system. All cultures have used herbs throughout history and it was an integral part of the development of modern civilization. Primitive man observed and appreciated the great diversity of plants available to him. Plants provided food, clothes, shelter and medicine and still play a vital role in rural villages of South Africa. The medicinal uses of plants and animals have been developed through trial and error. Herbal plants produce and contain a wide variety of chemical substances that act upon the body’s immune system. The medicinal properties of Ipomoea oblongata (E.Mey.ex Choisy) in this study was to determine the phytochemical content, antioxidant, anti-cancer and anti-diabetic properties of I. oblongata (Mothokho), a medicinal plant used by traditional medical practitioners of Thaba-Nchu in the Free State. The study was carried out in order to validate the ethnomedicinal claims made by the traditional medical practitioners and to evaluate the plant’s potential as a novel therapeutic agent. Twelve traditional medical practitioners of the Kopanang Dingaka Association were interviewed on the knowledge and use of I. oblongata (Mothokho). The phytochemical constituents of the plant were determined using standard screening methods. Testing for antioxidant properties (free radical scavenging activity) was carried out by means of the 2, 2- diphenylpicrylhydrazyl (DPPH) assays. The Sulforhodamine B assay was used to screen for anti-cancer activity in breast (MCF7), colon (HCT116) and prostate (PC3) cancer cell lines. Glucose uptake in C2C12 muscle cells was used to evaluate the anti- iii diabetic potential of I. oblongata. Methanol extract was fractionated using Ultra-Pure Liquid Chromatography (UPLC) to identify active compounds. The traditional medical practitioners cited the plant roots as the main ingredient in the treatment of respiratory infections, sexually transmitted infections, postnatal womb treatments, inflammation, arthritis, wounds and cancer. The methanol extract of I. oblongata showed remarkable (99.03%, 98.39, 71.31%) antioxidant potential in all triplicates tested which explains its use in oxidative stress-related diseases such as arthritis and cancer. Phytochemical tests showed the presence of carbohydrates, glycosides, steroids, terpenoids, alkaloids, flavonoids and tannins. However, the extracts were inactive against the cancer cell lines used. Glucose uptake by the C2C12 muscle cells was increased by over 150% and was comparable to that of insulin and metformin, suggesting good anti-diabetic activity. Predicted compounds found were dihydroquercetin pentaacetate, actinorhodin and actinorhodine, using Chemspider analysis. These are possible compounds that could be found in the Ipomoea oblongata extracts when using nuclear magnetic resonance (NMR) to determine the structure and names of the active compounds. Ethnobotanical uses of Ipomoea oblongata were found to have a link with other ethnobotanical studies to capture the indigenous knowledge, culture and therapeutic uses. The phytochemical results of Ipomoea oblongata can contribute to the knowledge of new drug development as indicated by literature (Harbone; 1973, Soforowa, 1993; De et al., 2010) that plants contain phytochemical contents and phenolic compounds that are valuable as medicinal properties. Methanolic extract of Ipomoea oblongata presented high levels of antioxidant activity respectively, which can become a potential iv antimicrobial agent and remedy oxidative stress related diseases. The anticancer properties were inactive and the cytotoxicity levels were found to below in I. oblongata. Low levels of cytotoxicity allow medicinal plants to be consumed by humans with fewer side effects however doses must be standardized. High levels of glucose uptake in muscle cells were indicated from the aqueous extract of Ipomoea oblongata, therefore showing great potential as an antidiabetic agent. Many medicinal plants have been validated as antidiabetic therapeutic agents in South Africa and the world. New treatment of cancer, diabetes, bacterial and viral infections is required from natural products that are cost effective with minimal side effects that can be used in health care systems. There is a correlation between the medicinal properties found in I. oblongata and the ethnomedicinal uses cited by the traditional medical practitioners. The plant I. oblongata has six ethno-pharmaceutical uses. I. oblongata is a good source of anti-diabetic and antioxidant agents that can be developed further. However the plant is not a good source of anticancer properties. Future research will be into the isolation and identification of the active compounds.

Traditional healers are one among professionals that are viewed as the most significant people in African Society. They are accorded the highest esteem because of the role they play in saving people’s lives and promoting cultural aspects such as ukuhlonipha amasiko in isiZulu. It should be remembered that this word ukuhlonipha which means to respect, is used across cultures to maintain peace within family and society. It all depends on which culture promotes it more than others. It is sociolinguistic truism that ukuhlonipha is essentially linked to the ubuntu philosophy of African people. However, traditional healers more than other people follow ukuhlonipha culture in numerous ways, especially when they meet, being consulted and during traditional ceremonies. They serve and promote what in IsiZulu is known as ukuhlonipha, which to the uninitiated and sometimes even to the scholars of this philosophy is a mystery. It is for this reason and others that people view them as the soul of society. This study looks at relevant sociolinguistic theories especially the politeness theory to analyze most of the hlonipha features shown by traditional healers, apprentice and people during consultation. / African Languages / M.A. (African Languages)