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Proper design of bridge structures requires an appreciation for the possible failure mechanisms that can develop over the lifetime of the bridge, many of which are related to natural hazards. For example, scour is one of the most common causes of bridge failures. Scour occurs due to the erosion of soil and sediment within a channel with flowing water. During a flood event, the extent of scour can be so great that it can destabilize an existing bridge structure. In order to evaluate the scour potential of a bridge, it is necessary to have information regarding the substructure, particularly the bridge foundations. However, as of 2011 there are more than 40,000 bridges across United States with unknown foundations. Generally for these bridges there are no design or as-built plans available to show the type, depth, geometry, or materials incorporated into the foundations. Several non-destructive testing (NDT) methods have been developed to evaluate these unknown foundations. The primary objective of this research is to identify the most current and widely used NDT methods for determining the embedment depth of unknown bridge foundations and to compare these methods to an ultrasonic P-wave reflection imaging system. The ultrasonic P-wave reflection system has tremendous potential to provide more information and address several short-comings of other NDT methods. A laboratory study was initiated to explore various aspects related to the P-wave system performance, in order to characterize the limitations of the system in evaluation of unknown foundations prior to deployment in field studies. Moreover, field testing was performed using the P-wave system and a number of the current NDT methods at two selected bridge foundations to allow comparison between the results. / Civil Engineering

The overall objective of this research was to understand the mechanims by which growth hormone (GH) regulates insulin-like growth factor-I (IGF-I) gene expression in liver. Previous studies have suggested that GH regulation of IGF-I gene expression in liver is mediated by binding of the transcription factor signal transducer and activator of transcription (STAT) 5 to four binding sites located distantly from the IGF-I promoter. The first specific objective of this research was to determine whether additional STAT5 binding sites were involved in GH stimulation of IGF-I gene expression in liver. Sequence analysis of 170 kb of mouse genomic DNA revealed nineteen consensus STAT5 binding sequences corresponding to fourteen ~200 bp chromosomal regions that were conserved in the corresponding human DNA sequence. Eight of these chromosomal regions were able to mediate STAT5 activation of reporter gene expression in cotransfection experiments. Two of these chromosomal regions corresponded to those previously identified. Gel-shift assays indicated that the eight new STAT5 binding sites and three of the four previously identified STAT5 binding sites could bind GH-activated STAT5 from mouse liver. Together, these results suggest that GH stimulation of IGF-I gene transcription in the mouse liver may be mediated by at least eleven STAT5 binding sites located distantly from the IGF-I promoter. In a previous study, I found that liver expression of liver-enriched transcription factor hepatocyte nuclear factor 3γ (HNF-3γ) was increased by GH in cattle. Therefore, the second specific objective of this research was to determine how GH stimulates HNF-3γ gene expression and whether the increased HNF-3γ mediates GH stimulation of IGF-I gene expression in bovine liver. Sequence analysis of the bovine HNF-3γ promoter revealed the presence of two putative binding sites for STAT5. The proximal putative STAT5 binding site appears to be conserved in other mammals. Chromatin immunoprecipitation (ChIP) assays demonstrated that GH increased the binding of STAT5 to the HNF-3γ promoter in bovine liver and that this binding was associated with increased HNF-3γ expression. Gel-shift assays demonstrated that the proximal STAT5 binding site in the HNF-3γ promoter could bind GH-activated STAT5 from bovine liver. Cotransfection analyses showed that the proximal STAT5 binding site was necessary for the HNF-3γ promoter to be activated by GH. The promoter of the bovine IGF-I gene contains three putative HNF-3 binding sites that seem to be evolutionarily conserved. ChIP assays indicated that GH stimulated the binding of HNF-3γ to the IGF-I promoter in bovine liver. Gel-shift assays showed that one of the putative HNF-3 binding sites could bind HNF-3γ protein from bovine liver. Co-transfection analyses demonstrated that this HNF-3 binding site was necessary for HNF-3γ activation of reporter gene expression from the IGF-I promoter. In summary, the results of this dissertation research suggest that GH-activated STAT5 directly stimulates IGF-I gene transcription in liver by binding to at least eleven distantly located STAT5 binding sites in the IGF-I locus and indirectly stimulates IGF-I gene transcription by enhancing HNF-3γ gene expression in the liver. / Ph. D.

Η παρούσα διδακτορική διατριβή εστιάστηκε στη μελέτη συστημάτων ακουστικής εκπομπής για απευθείας αναπαραγωγή ψηφιακού ήχου. Η ερευνητική διαδικασία βασίστηκε στον προσδιορισμό και βελτίωση των δυνατοτήτων δύο διαφορετικών υλοποιήσεων ακουστικής μετατροπής: i. Ένα υβριδικό πρωτότυπο θερμοακουστικό στοιχείο και ii. Μια συστοιχία 32 ηλεκτροδυναμικών μεγαφώνων σχεδιασμένη, ώστε να αναπαράγει ψηφιακά ηχητικά σήματα. Η θερμοακουστική μετατροπή προσφέρει μια εναλλακτική τεχνική για υλοποιήσεις ακουστικών στοιχείων. Είναι βασισμένη στο μετασχηματισμό των διακυμάνσεων της θερμικής ενέργειας σε ακουστικό κύμα που προκαλούνται από τη ροή του ηλεκτρικού σήματος ήχου σε μια συσκευή στερεάς κατάστασης που λειτουργεί χωρίς τη χρήση οποιουδήποτε κινούμενου τμήματος ή μηχανισμού. Η υλοποίηση αυτής της τεχνικής ηχητικής αναπαραγωγής, μελετάται με τη χρήση ενός πρωτότυπου μετατροπέα ο οποίος αναπτύχθηκε πάνω σε πλακέτα κρυσταλλικού πυριτίου (silicon wafer). H απόδοση της συσκευής αυτής βελτιώνεται ιδίως όσον αφορά στις μη γραμμικές παραμορφώσεις που προσθέτει ο φυσικός μηχανισμός κατά την αναπαραγωγή των ακουστών συχνοτήτων. Για τις ανάγκες της ερευνητικής μελέτης κατασκευάσθηκε εξειδικευμένο στάδιο οδήγησης, ενώ επίσης αναπτύχθηκαν εργαλεία που προσομοιώνουν την απόδοση αυτών των συσκευών. Οι ψηφιακές συστοιχίες μεγαφώνων (DLAs) σήμερα βασίζονται σε μικρούς μετατροπείς κινούμενου πηνίου για την ανακατασκευή ακουστικών σημάτων από ροές ψηφιακού ήχου. Τα σημαντικά ζητήματα απόδοσης για τα συστήματα αυτά αναλύονται από την παρούσα διατριβή, με στόχο να ερμηνευθεί η απόκριση συχνότητας και οι ρυθμοί των διακριτών (on/off) μεταβάσεων των μεγαφώνων, εξαιτίας των ψηφιακών σημάτων. Λεπτομερείς προσομοιώσεις που επιτρέπουν την πραγματοποίηση συγκρίσεων για μια πανομοιότυπη συστοιχία 32 μετατροπέων η οποία τροφοδοτείται από αναλογικά σήματα, σε παρόμοια τοποθέτηση και ενεργοποίηση των στοιχείων. Οι μελέτες αυτές παράγουν πρωτότυπα αποτελέσματα για τις απαιτήσεις σε ηλεκτρική ενέργεια και την ευαισθησία της συστοιχίας, καταλήγοντας στο συμπέρασμα ότι αυτά τα δύο συστήματα επιτυγχάνουν συγκρίσιμες επιδόσεις. / The present Phd Thesis is focused on the study of acoustic transduction systems for direct digital audio signal emission. The research process was based on the evaluation and optimization of the behavior of two different implementations: i. A novel hybrid thermoacoustic device and ii. A loudspeaker array consisting of 32 moving coil speakers designed for digital audio reproduction. Thermoacoustic transduction offers an alternative technique for transducer implementations, based on the transformation of thermal energy fluctuations into sound after the direct application of the electrical audio signal on a solid state device which operates without the use of any moving/mechanical components. Here, an implementation of this sound generation technique is studied based on a prototype developed on silicon wafer and its performance is optimised, especially with respect to non-linear distortions within the audio band. For the purposes of the research study a specialised driving circuit was constructed and also the appropriate tools were developed to simulate the performance of these devices. Digital loudspeaker arrays currently are based on small moving-coil speakers to reconstruct acoustic signals out of binary audio streams. An overview of significant performance issues for such systems is given here to explain frequency response and speaker discrete transition rates due to the digital data. Detailed simulations provided comparisons for a 32-speaker DLA with similar arrangements of speakers driven by analogue signals. These tests produce novel results for electrical power requirements and array sensitivity, concluding that these two systems achieve comparable performance.

International Telemetering Conference Proceedings / October 27-30, 1997 / Riviera Hotel and Convention Center, Las Vegas, Nevada / This paper describes a Smart Sensor Network System for applications requiring sensors connected in a multidrop configuration in order to minimize interconnecting cables. The communication protocol was optimized for high speed data collection. The Smart Sensor Network System was developed with the following goals in mind: cost reduction, reliability and performance increase.

In biomechano-transducing, cellular generated tension can be measured by soft substrates based on polymers but these techniques are limited either by spatial resolution or ability to detect localised cell traction forces (CTF) due to their non-linear viscous behaviour under shear rates. A newly developed cell traction force transducer system based on cholesteryl ester lyotropic liquid crystals (LCTFT) was developed to sense localised traction forces of human keratinocyte cell lines (HaCaTs), in which the length of the deformation line induced represents the intensity of the CTF exerted. The physical properties of the cholesteryl ester based lyotropic liquid crystals (LLC) were characterised by using polarising microscopy, rheology, atomic force microscopy (AFM) based nano-indentation, spherical indentation, and micro-tensile tests. The interactions of LLC with cells were studied by using cell viability studies, cytochemical treatments, widefield surface plasmon resonance (WSPR) microscopy and various immuno-staining techniques. The results show that LLC is thermally stable (0-50 °C) and linearly viscoelastic below 10% shear strain at shear rates of < 1 s⁻¹. AFM nano and spherical indentations show a good agreement on the Young's modulus of both determined at ~110 kPa which is close to the elastic modulus of the epidermis. The Poisson's ratio of LLC was determined at ~0.58 by using micro tensile tests. The biophysical interaction studies indicated that LLC is biocompatible and allowed cell attachment. Cell relaxation technique by cytochalasin-B treatment suggested that the attachment and contraction of cells on LLC was due to the contractile activity of actin cytoskeletons that are mediated by focal adhesions. The staining experiments showed that cells consistently expressed the same suites of integrins (α2, α3, α5 and β1) and ECM proteins (collagen type IV, laminin and fibronectin) on both glass and LLC coated substrates. Interfacial interaction of cells with LLC observed via the staining of actin and vinculin, and WSPR imaging suggest the association of marginal actin filaments and focal adhesions in attaching HaCaT cells to the LLC. Linear static analysis applied in the Finite Element model of focal adhesion-LC confirmed the compressive force patterns induced by cells. By applying cell relaxation techniques and Hooke's theorem, the force-deformation relationships of the LLC were derived and used for direct quantification of CTF in culture. The sensitivity of the LCTFT was implied by a wide range of CTF (10 - 140 nN) measured at high resolutions (~2 μm). Nonetheless, a custom-built cell traction force measurement and mapping software (CTFM) was developed to map CTF of single cells. Reliability of the LCTFT was evaluated by using a known pharmacological active cytokine, TGF-β1, in inducing contraction of human keratinocytes. This study inferred internal consistency and repeatability of the LCTFT in sensing contraction responses of HaCaT cells in a concentration dependent manner of TGF-β1. The overall LCTFT and CTFM software had shown good potential for use in the study of contraction and migration of keratinocytes.

Within the context of natural language processing, a lemmatiser is one of the most important core technology modules that has to be developed for a particular language. A lemmatiser reduces words in a corpus to the corresponding lemmas of the words in the lexicon. A lemma is defined as the meaningful base form from which other more complex forms (i.e. variants) are derived. Before a lemmatiser can be developed for a specific language, the concept "lemma" as it applies to that specific language should first be defined clearly. This study concludes that, in Setswana, only stems (and not roots) can act independently as words; therefore, only stems should be accepted as lemmas in the context of automatic lemmatisation for Setswana. Five of the seven parts of speech in Setswana could be viewed as closed classes, which means that these classes are not extended by means of regular morphological processes. The two other parts of speech (nouns and verbs) require the implementation of alternation rules to determine the lemma. Such alternation rules were formalised in this study, for the purpose of development of a Setswana lemmatiser. The existing Setswana grammars were used as basis for these rules. Therewith the precision of the formalisation of these existing grammars to lemmatise Setswana words could be determined. The software developed by Van Noord (2002), FSA 6, is one of the best-known applications available for the development of finite state automata and transducers. Regular expressions based on the formalised morphological rules were used in FSA 6 to create finite state transducers. The code subsequently generated by FSA 6 was implemented in the lemmatiser. The metric that applies to the evaluation of the lemmatiser is precision. On a test corpus of 1 000 words, the lemmatiser obtained 70,92%. In another evaluation on 500 complex nouns and 500 complex verbs separately, the lemmatiser obtained 70,96% and 70,52% respectively. Expressed in numbers the precision on 500 complex and simplex nouns was 78,45% and on complex and simplex verbs 79,59%. The quantitative achievement only gives an indication of the relative precision of the grammars. Nevertheless, it did offer analysed data with which the grammars were evaluated qualitatively. The study concludes with an overview of how these results might be improved in the future. / Thesis (M.A. (African Languages))--North-West University, Potchefstroom Campus, 2006.

Résumé : Les systèmes de surveillance de santé structurale sont proposés pour la détection d’endommagement dans les infrastructures qui dépassent leur durée de vie en utilisant les ondes guidées (GW). Les ondes guidées peuvent parcourir de longues distances et sont sensibles à une variété d’imperfections. Les transducteurs piézoélectriques sont communément utilisés pour générer et mesurer les ondes guidées dans des structures minces. Comme la détection du défaut et sa localisation sont souhaitées, la nature de la génération des ondes guidées sous forme de plusieurs modes implique une complexité supérieure dans le traitement du signal. Pour remédier à cette limitation, une nouvelle méthode est présentée ici pour la génération des ondes guidées par sélection de mode, et un nouveau transducteur piézoélectrique est ensuite conçu, fabriqué et testé. Tout d'abord, la génération des ondes guidées par optimisation systématique du profil interfacial de la contrainte de cisaillement en mode sélectif est étudiée. En utilisant le principe de superposition, une méthode d'analyse est d'abord développée pour la modélisation de la génération des ondes guidées par un nombre fini de segments de contrainte de cisaillement uniforme, chacun contribuant à un profil élémentaire d’une contrainte constante de cisaillement. Sur cette base, deux fonctions coût sont définies afin de minimiser les modes indésirables et amplifier le mode sélectionné et le problème d'optimisation est résolu avec un cadre d'optimisation d’algorithme génétique parallèle. Les avantages de cette méthode par rapport à d'autres approches de conception de transducteurs classiques sont (1) la contrainte de cisaillement peut être explicitement optimisée à la fois pour exciter un mode et supprimer d'autres modes indésirables, (2) la taille de la zone d'excitation n’est pas limitée et l’excitation en mode sélectif est toujours possible, même si la largeur d'excitation est inférieure à toutes les longueurs d'onde excitées, et (3) la sélectivité est accrue et la largeur de bande est étendue. La méthode analytique et les fonctions coût sont ensuite développées pour concevoir un transducteur piézoélectrique à éléments multiples (MEPT) simple et performant. Une méthode numérique est tout d'abord mise au point pour extraire la contrainte interfaciale entre un seul élément piézocéramique et une structure d'accueil et ensuite utilisée comme entrée d'un modèle analytique pour prédire la propagation des ondes guidées à travers l'épaisseur d'une plaque isotrope. Deux nouvelles fonctions coût sont proposées pour optimiser la contrainte de cisaillement interfaciale pour supprimer le(s) mode(s) indésirable(s) et maximiser un mode désiré. Simplicité et faible coût de fabrication sont deux principales cibles visées dan la conception du MEPT. Un prototype TPEM est ensuite fabriqué à l'aide de micro-usinage laser. Une procédure expérimentale est présentée afin de valider les performances de la TPEM comme une nouvelle solution pour la génération des ondes guidées en mode sélectif. Des essais expérimentaux illustrent la forte capacité du TPEM pour la génération des ondes guidées en mode sélectif, puisque le mode indésirable est supprimé par un facteur allant jusqu'à 170 fois par rapport aux résultats obtenus avec un seul piézocéramique. / Abstract : Structural Health Monitoring (SHM) systems are being proposed for damage detection of infrastructures that exceed their life using ultrasonic Guided waves (GWs). GWs can travel over long distances and are sensitive to variety of defects. Piezoelectric transducers (PZTs) are commonly used to generate and measure GWs in plate-like structures. As damage detection and localization is sought, the multi-mode nature of GW generation involves higher complexity in signal processing. To overcome this limitation, a new method is presented here for modeselective GW generation, and a novel mode-selective PZT is then designed, manufactured and tested. First, mode-selective generation of GWs by systematic optimization of the interfacial shear stress profile is investigated. Using the superposition principle, an analytical method is first developed for modeling GWs generation by a finite number of uniform shear stress segments, each contributing with a constant elementary shear stress profile. Based on this, two cost functions are defined in order to minimize the undesired modes and amplify the selected mode and the optimization problem is solved with a parallel Genetic Algorithm (GA) optimization framework. Advantages of this method over more conventional transducers tuning approaches are that (1) the shear stress can be explicitly optimized to both excite one mode and suppress other undesired modes, (2) the size of the excitation area is not constrained and mode-selective excitation is still possible even if excitation width is smaller than all excited wavelengths, and (3) the selectivity is increased and the bandwidth extended. The analytical method and objective functions are then developed to design a novel and costeffective multi-element piezoelectric transducer (MEPT). A numerical method is first developed to extract the interfacial stress between a single piezoceramic element and a host structure and then used as the input of an analytical model to predict the GW propagation through the thickness of an isotropic plate. Two novel objective functions are proposed to optimize the interfacial shear stress for both suppressing unwanted mode(s) and maximizing a desired mode. Simplicity and low manufacturing cost are two main targets driving the design of the MEPT. A prototype MEPT is then manufactured using laser micro-machining. An experimental procedure is presented to validate the performances of the MEPT as a new solution for mode-selective GW generation. Experimental tests illustrate the high capability of the MEPT for mode-selective GW generation, as unwanted mode is suppressed by a factor up to 170 times compared with the results obtained with a single piezoceramic.

Les réseaux de nanotrous sont des structures plasmoniques ayant un énorme potentiel en tant que transducteurs pour la conception de biocapteurs. De telles structures sont prometteuses pour l’élaboration de biocapteurs capable d’effectuer du criblage à haut débit. L’intérêt de travailler avec des réseaux de nanotrous est dû à la simplicité d’excitation des polaritons de plasmons de surface en transmission directe, à la sensibilité et à la facilité de fabrication de ces senseurs. L’architecture de tels réseaux métalliques permet la conception de nanostructures ayant de multiples propriétés plasmoniques. L’intensité, la signature spectrale et la sensibilité du signal plasmonique sont grandement affectées par l’aspect physique du réseau de nanotrous. L’optimisation du signal plasmonique nécessite ainsi un ajustement du diamètre des trous, de la périodicité et de la composition métallique du réseau. L'agencement de l'ensemble de ces paramètres permet d'identifier une structure optimale possédant une périodicité de 1000 nm, un diamètre des nanotrous de 600-650 nm et un film métallique ayant une épaisseur de 125 nm d'or. Ce type de transducteur a une sensibilité en solution de 500-600 nm/RIU pour des bandes plasmoniques situées entre 600-700 nm. L'intérêt de travailler avec cette structure est la possibilité d'exciter les plasmons de polaritons de surface (SPPs) selon deux modes d'excitation : en transmission exaltée (EOT) ou en réflexion totale interne par résonance des plasmons de surface (SPR). Une comparaison entre les propriétés plasmoniques des senseurs selon les modes d'excitation permet de déterminer expérimentalement que le couplage de la lumière avec les ondes de SPP de Bloch (BW-SPPs) en transmission directe résulte en un champ électromagnétique davantage propagatif que localisé. D'un point de vue analytique, la biodétection de l'IgG en SPR est 6 fois plus sensible par rapport au mode EOT pour une même structure. Une étude du signal plasmonique associé au BW-SPP pour un certain mode de diffraction démontre que la distance de pénétration de ces structures en EOT est d'environ 140 nm. La limite de détection de l'IgG humain pour un réseau de nanotrous de 1000 nm de périodicité est d'environ 50 nM en EOT. Ce mémoire démontre la viabilité des réseaux de nanotrous pour effectuer de la biodétection par criblage à haut débit lors de prochaines recherches. L'investigation de l'effet de l'angle d'excitation en transmission exaltée par rapport au signal plasmonique associé au mode (1,0) d'un réseau de nanotrous de 820 nm d'or démontre que la sensibilité en solution n'est pas proportionnelle à la sensibilité en surface du senseur. En fait, une optimisation de l'angle d'incidence pour le mode (1,0) de diffraction des BW-SPP permet d'amplifier la sensibilité en surface du senseur jusqu'à 3-fois pour un angle de 13,3°. Ce mémoire démontre ainsi la nécessité d'optimiser l'angle d'excitation et les propriétés physiques du senseur afin de développer un transducteur de grande sensibilité basé sur l'excitation en transmission de réseaux de nanotrous. / This research aims at developing a multiplexed biosensor for protein detection based on the nanohole array technology. Gold nanohole arrays exhibit distinct plasmonics properties depending on the excitation mode of the surface plasmon polaritons (SPPs). The interest of working with nanohole arrays is related to their high sensitivity, ease of fabrication and simple setup of excitation in transmission. The architecture of nanohole arrays leads to a nanostructure having multiple plasmonics properties. The intensity, the spectral signature and the sensitivity of the plasmonic signal were highly affected by the shape of the nanohole arrays. Varying the diameter of the holes, the periodicity and the metallic composition of the array were used to optimize the plasmonic signal. The optimal structure was found to have a periodicity of 1000 nm, a diameter of 600-650 nm and a metallic film with a thickness of 125 nm of gold. Such a transducer exhibits a bulk refractive index sensitivity of 500-600 nm/RIU for plasmonic bands absorbing around 600-700 nm. Surface plasmon resonance (SPR) in the Kretschmann configuration and enhanced optical transmission (EOT) mode were compared using large gold nanohole arrays (1000 nm periodicity, 600 nm diameter and 125 nm depth) in order to assess their relative analytical performance. Biodetection of IgG was found to be 6 times more sensitive with SPR in the Kretschmann configuration than in EOT mode for the same structure. The decay length of the electromagnetic field in EOT mode was determined experimentally to be around 140 nm with a layer-by-layer polyelectrolyte deposition. This results suggests that the plasmonic properties of EOT for nanohole arrays is much more associated to a Bloch wave SPPs mode rather than a localized SPR. Variation of the incident angle of excitation of the BW-SPPs in transmission leads to a higher surface sensitivity for the (1,0) diffraction mode for gold nanohole arrays of 820 nm periodicity. Optimization of the physical properties and the excitation angle of the nanohole arrays is essential in order to develop a transducer having a potential towards multiplexed biosensors.