Thèse doctorat : Biologie Végétale et Forestière : Nancy 1 : 2006. / Titre provenant de l'écran-titre.

Zugl.: Saarbrücken, Univ., Diss., 2007

Zugl.: Köln, Univ., Diss., 2007

Contemporary, mainstream moral education in North America teaches students to be compliant rather than skilled in moral consciousness. The Myths of Moral Education, illustrated as ‘Necessity,’ ‘Neutrality,’ ‘Liberal Subjectivity,’ ‘The Good,’ and ‘Dissent,’ maintain a system of control. Through a Foucaudian lens, I address, within common teaching practices, the presence of productive power and oppression, possibilities for subversive action, and propose an abandonment of liberal moral education. Counter-arguments are explored in a discussion on the control/chaos dichotomy and a dismantling of dissent as a tool of a liberal paradigm. Using a pedagogical framework influenced by bell hooks and Paulo Freire, I argue that what must occur, before taking on pedagogical change, is a teacher transformation through a Praxis of ‘Letting Go’. Finally, I present an alternative to education’s preoccupation with compliance through Pedagogies of Resistance; a three-part approach that assesses power, promotes compassion, and creates space for empowerment. / Graduate / 0727 / 0998 / 0530 / lcharvey@uvic.ca

The Best MPhil Thesis in the Faculties of Dentistry, Engineering, Medicine and Science (University of Hong Kong), Li Ka Shing Prize / published_or_final_version / Pathology / Master / Master of Philosophy

An understanding of plant pathogen-host interactions is essential to design efficient strategies to control disease in crops. Magnaporthe oryzae, an ascomyceteous fungus and causal agent of rice blast disease, is a model organism to study host-microbe interactions. The overall aim of this dissertation project was to identify genes involved in pathogenicity through the construction and characterization of a random insertional mutagenesis library. In order to saturate the genome with DNA inserts, a collection of >54,000 insertion lines of the M. oryzae strain 70-15 was generated via two transformation methods, PEG/CaCl2 (polyethylene glycol)-mediated protoplast transformation and Agrobacterium tumefaciens-mediated transformation. The first part of this dissertation describes the optimization of both transformation approaches, compares their efficiency and provides a description of the high-throughput processing and phenotypic analysis of the insertion lines. An in vitro appressorium assay of 12,000 T-DNA insertion strains allowed the identification of 135 lines that were classified as morphologically or functionally different than wild-type. Rice infection assays demonstrated that 112 of these strains exhibited defects in pathogenicity.The second part of this dissertation project analyzed the T-DNA integration patterns in a subset of pathogenicity mutants. This section aimed to identify the disrupted genes via recovery of M. oryzae sequences adjacent to the sites of T-DNA insertion. Genomic mapping of 61 T-DNA insertions in pathogenicity mutants via rescuing M. oryzae chromosomal T-DNA flanking sequences using inverse PCR resulted in the identification of 22 conserved hypothetical genes with predicted function, 11 predicted open reading frames without a GenBank significant match, two unannotated regions of the genome assembly and seven intergenic regions. The final part of this dissertation describes the characterization of a M. oryzae pathogenicity mutant that contains a T-DNA insertion in the upstream region of two divergently transcribed genes that encode the vacuolar type-ATPase subunit c`` and the general transcription factor TFIIA subunit γ. Genetic complementation demonstrated the insertion of the T-DNA in the promoter region of the general transcription factor TFIIA subunit γ is responsible for observed defects in conidiation, appressorium morphogenesis, and appressorium function. This is the first report relating the function of TFIIA subunit γ to pathogenicity.

There are thousands of students being examined in universities each year. Most of those knowledge inspections are fulfilled in written form. It means that a lot of works must be checked. So many lecturers like to do examinations in a form of testing. Test - a systematic procedure that helps to measure some model of behaviour of individual for valuing that behaviour according to particular standards, norms. Tests render quantitative evaluation of skills or studies advancement and show what is being learned. Testing helps to evaluate students faster or more objective and to minimize the probability of the false evaluation. But it takes quite a lot of time to prepare tests. It’s difficult to create problematic or simulation situations in the paper test. The better way is to use computerised testing systems. Department of Practical Informatics uses small plane and 3-D geometric transformations testing system, which is pretty limited and works only in AutoCAD environment. There is no possibility of saving students’ results. This system fulfils only a part of lecturer’s requirements. The goal of the work is to create plane and 3-D geometric transformations testing (creation of tests and testing process itself) system according to definite specification. The tasks which help to reach the goal are: to review and to analyse all plane and 3-D geometric transformations; to formulate the task of the test creation and testing process support for geometric transformations; to formulate... [to full text]

This paper focuses on the concept of a subjectivized grammatical metaphor and its practical application in the translation of English fiction. At first, it explains the theoretical concept of a grammatical metaphor in general, following Halliday and other scholars. Later it gives the wide array of translation transformations of subjectivized grammatical metaphor to illustrate this theoretical overview with the examples of its practical application. / Šis darbas pristato gramatinės metaforos savoką ir atvejus, kai ji eina sakinyje veiksniu, bei jų vertimą į lietuvių kalbą iš anglų grožinės literatūros kūrinių. Darbo pradžioje aptariama teorinė gramatinės metaforos sąvoka, remiantis Halliday ir kitais mokslininkais. Antroje darbo dalyje nagrinėjami konkretūs gramatinės metaforos vertimo pavyzdžiai, iliustruojantys šią teorinę apžvalgą.

Oncogenically transnformed mammalian cells irrespective of their origin synthesize and secrete osteopontin (OPN), a sialic acid rich, adhesive, phosphoglycoprotein, not only in excessive amounts but also in different molecular forms, as compared to their non-transformed counterparts. It has been postulated that OPN has important functional roles in oncogenesis, but its mechanism of action remains obscure. In the present study this question was addressed by using Rat-1 cells transformed by a temperature-sensitive mutant of Rous sarcoma virus (tsB77 cells) which secrete two discrete molecular forms of OPN, a 69-kDa OPN at the non-permissive temperature (41°C and a 62-kDa form at the permissive temperature (34°C). Investigations were aimed to determine how the two isoforms of OPN secreted by transformed and non-transformed cells originate, whether the two forms have different functional properties, and the effects of specific inhibition of OPN synthesis on the transformed state of the cells. The latter was achieved by transfecting tsB77 cells with antisense OPN cDNA at the permissive temperature. Immunoprecipitation, V8 protease mapping, tryptic peptide analysis, and thrombin digestion confirmed that 62-kDa and 69-kDa proteins are two isoforms of OPN. It was also observed that tsB77 cells at both temperatures transcribe a single 1.6 kb OPN mRNA and contain only the 69-kDa OPN intracellularly, suggesting that 69-kDa OPN is modified to its 62-kDa form prior to or immediately after secretion by cells at 34ºC. Proteolytic cleavage, differential phosphorylation, or lack of N- or O-linked carbohydrates as the possible mechanism for the generation of 62-kDa OPN were ruled out, but it was observed that 62-kDa OPN contains significantly reduced levels of sialic acid residues, as compared to its 69-kDa form. The binding assays using 32P-labeled OPN revealed that only the 69-kDa OPN, not its 62-kDa form, undergoes receptor-mediated localization on the cell surface,

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