In the past few years, there has been a growth in Unmanned Aircraft Systems (UAS) numbers in segregated airspace. However, although there is an interest in integrating large UAS into non-segregated airspace, the safety challenges on its integration arise from the inclusion of new ways of reaching unsafe states into the airspace. Furthermore, Air Traffic Controllers (ATCo) aim to o?er appropriate levels of safety and efficiency and to solve issues present in complex situations. Although the UAS technology may be used in di?erent situations and brings several advantages to the airspace (e.g. efficiency), it may bring uncertainties due to the fact that ATCos may not be familiar with them. Throughout the years, this impact may be lower then it is nowadays due to the fact that the present lack of familiarity in the relationship between UAS and ATCo contributes to higher workload levels. Furthermore, Terminal Maneuvering Area (TMA), which composes the controlled airspace and in which the final sector in contained, is a critical control area normally established at the confluence of Air Traffic Service (ATS) routes in which the aircraft tend to be closer to each other. Thus, operations in this particular area are conducted carefully and, in order to achieve desirable levels of safety and efficiency, standard procedures are established. In some cases, however, standard procedures cannot be followed and the sequencing of the aircraft during the approach, which is a highly challenging task due to complex maneuvers constraints, must be performed by the ATCo in a manner to respect the minimum separation of aircraft and to avoid flights through cumulonimbus (CB). Finally, the main goal of defining a final arrival segment is to deliver the set of aircraft from the final sector of the TMA to the final phase of its landing procedure, i.e., the final approach, considering the operation efficiency and safety. The main objective of this research is to propose a parallel swarm-based method for optimizing final aircraft arrival segments design, i.e., routes that connects the final sector to the Initial Approach Fix (IAF), considering the UAS presence. This is conducted from two perspectives: ATCo workload, which is related to safety, and sequencing duration, which is related to efficiency. Furthermore, di?erent phases of UAS integration are considered, i.e., from early stages of its integration to a mature stage of its operation by means of the Technology Maturity Level (TML) usage, which is a scale that measure the familiarity between the ATCo with the aircraft. Finally, the solutions consider airspace restrictions such as minimum separation between aircraft and bad weather conditions, i.e., the presence of cumulonimbus (CB). The experiments conducted show that this approach is able to build safe and efficient solution even in situations with a high number of aircraft. / Nos últimos anos, houve um crescimento, no espaço aéreo segregado, nos números do Veículos Aéreos Não-Tripulados (VANT). No entanto, embora exista interesse em integrar grandes VANT em espaço aéreo não-segregado, os desafios de segurança decorrem da inclusão de novas formas de alcançar estados inseguros no espaço aéreo (ATCo) tem como objetivo oferecer níveis adequados de segurança e eficiência e resolver problemas presentes em situações complexas. Embora VANTs possam ser usados em diferentes situações e trazem várias vantagens para o espaço aéreo (por exemplo, eficiência), podem trazer incertezas devido ao fato de que os ATCos não estão familiarizados com essa tecnologia. Ao longo dos anos, esse impacto pode ser menor, e atualmente a falta de familiaridade na relação entre VANT e ATCo contribui para níveis mais altos de carga de trabalho. Além disso, a Área Terminal (TMA), que compõe o espaço aéreo controlado, é uma área de controle crítico normalmente estabelecida na confluência de rotas do Servi¸co de Tráfego Aéreo (ATS), nas quais as aeronaves tendem a estar mais próximas umas das outras. Assim, as operações nesta área particular são realizadas com cuidado e, para alcançar níveis desejáveis de segurança e eficiência, os procedimentos padrão são estabelecidos. Em alguns casos, no entanto, procedimentos padrão não podem ser seguidos e o sequenciamento da aeronave durante a aproximação, que é uma tarefa desafiadora por conta das restrições de manobras complexas, deve ser realizada pelo ATCo de forma a garantir separação mínima entre aeronaves e evitar voos através de cumulonimbus (CB). Finalmente, o principal objetivo de definir um segmento de chegada final ´e entregar o conjunto de aeronaves do setor final, da TMA, para a fase final do seu procedimento de pouso, ou seja, a aproximação final, considerando a eficiência e a segurança da operação. O objetivo desta pesquisa é propor um método paralelo baseado em enxame para otimizar o projeto final de segmentos de chegada de aeronaves, ou seja, rotas que conectem o setor final com o Fixo de Aproximação Inicial (IAF), considerando a presença de VANTs. Esse processo ´e conduzido a partir de duas perspectivas: a carga de trabalho do ATCo, que est´a relacionada à segurança, e a duração da sequenciamento, que está relacionado à eficiência. Além disso, são consideradas diferentes fases da integração de VANTs, ou seja, desde os primeiros estágios de sua integra¸c~ao at´e um estágio maduro de sua operação por meio do uso do Nível de Maturidade Tecnológica (TML), que é uma escala que mede a familiaridade entre o ATCo e a aeronave. Finalmente, as soluções consideram as restrições do espaço aéreo, como a separação mínima entre aeronaves e condições climáticas adversas, isto é, a presença de cumulonimbus (CB). Os experimentos realizados mostram que essa abordagem é capaz de criar soluções seguras e eficientes mesmo em situações com um grande número de aeronaves.

Modelos de arquitetura têm sido utilizados para permitir o desenvolvimento mais adequado e estruturado de sistemas, desde os mais simples até os mais complexos. A utilização desses modelos em sistemas embarcados, principalmente quando se trata de sistemas embarcados críticos, como é o caso de veículos aéreos não tripulados (VANT), visam a permitir conformidades de padrões, redução no tempo de produção, redução e facilidade no processo de manutenção e desenvolvimento. Sistemas embarcados críticos possuem requisitos específicos, tais como alta confiabilidade e resposta em tempo real, segurança e desempenho. A definição de um modelo arquitetural que permita que esses quesitos sejam levados em consideração, propicie o atendimento aos padrões, além de permitir o desenvolvimento correto e acelerado é inovador, permitindo que não só a comunidade científica venha a ter benefícios com a sua concepção, mas também a indústria brasileira possa ganhar. Nesse sentido, este trabalho desenvolveu um modelo de arquitetura para a interconexão de sistemas aéreos não tripulados (SANTs) em Unified Modeling Language (UML)/System Modelling Language (SysML) denominado LARISSA (Layered ARchitecture model for Interconnection of SystemS in uAs). Como resultado deste trabalho foi possível a modelagem e especificação completa de um SANT fazendo uso desse modelo e a realização de diversos experimentos que permitiram validar o LARISSA. Os experimentos, focados na parte de comunicação, permitiram a concepção de um simulador de redes de VANTs. Os resultados obtidos demonstraram a eficiência e a eficácia do modelo de arquitetura LARISSA, além da sua flexibilidade em permitir que diferentes experimentos possam ser realizados, o que auxilia na obtenção de dados que facilitam o processo de certificação desses VANTs. / Architecture models have been used to allow more adequate and structured development of systems, from the simplest to the most complex. The use of models in embedded systems are used to enable compliance standards, reduction in production time and ease of maintenance and development, especially when it comes to critical embedded systems such as unmanned aerial vehicles. Critical embedded systems have specific requirements, such as high reliability and real-time response, security and performance. The definition of an architectural model that deals with these requirements and standards and provides the service to standards allowing the correct and fast development is innovative and will provide benefits to the scientific and industrial communities. In this sense, this work developed an architectural model for the interconnection of Unmanned Aerial Systems (UAS) in Unified Modeling Language (UML)/System Modelling Language (SysML) called LARISSA (Layered ARchitecture model for Interconnection of SystemS in uAs). As a result of this development it was possible the modelling and specification of a complete UAS and the conduction of several experiments that allowed LARISSA validation. The experiments focused on the communication enabled the design of a UAV networks simulator. The results showed the efficiency and effectiveness of LARISSA architecture model, as well as its flexibility in carrying out different experiments, which helps in getting data to facilitate the certification process of these UAVs.

In the past few years, there has been a growth in Unmanned Aircraft Systems (UAS) numbers in segregated airspace. However, although there is an interest in integrating large UAS into non-segregated airspace, the safety challenges on its integration arise from the inclusion of new ways of reaching unsafe states into the airspace. Furthermore, Air Traffic Controllers (ATCo) aim to o?er appropriate levels of safety and efficiency and to solve issues present in complex situations. Although the UAS technology may be used in di?erent situations and brings several advantages to the airspace (e.g. efficiency), it may bring uncertainties due to the fact that ATCos may not be familiar with them. Throughout the years, this impact may be lower then it is nowadays due to the fact that the present lack of familiarity in the relationship between UAS and ATCo contributes to higher workload levels. Furthermore, Terminal Maneuvering Area (TMA), which composes the controlled airspace and in which the final sector in contained, is a critical control area normally established at the confluence of Air Traffic Service (ATS) routes in which the aircraft tend to be closer to each other. Thus, operations in this particular area are conducted carefully and, in order to achieve desirable levels of safety and efficiency, standard procedures are established. In some cases, however, standard procedures cannot be followed and the sequencing of the aircraft during the approach, which is a highly challenging task due to complex maneuvers constraints, must be performed by the ATCo in a manner to respect the minimum separation of aircraft and to avoid flights through cumulonimbus (CB). Finally, the main goal of defining a final arrival segment is to deliver the set of aircraft from the final sector of the TMA to the final phase of its landing procedure, i.e., the final approach, considering the operation efficiency and safety. The main objective of this research is to propose a parallel swarm-based method for optimizing final aircraft arrival segments design, i.e., routes that connects the final sector to the Initial Approach Fix (IAF), considering the UAS presence. This is conducted from two perspectives: ATCo workload, which is related to safety, and sequencing duration, which is related to efficiency. Furthermore, di?erent phases of UAS integration are considered, i.e., from early stages of its integration to a mature stage of its operation by means of the Technology Maturity Level (TML) usage, which is a scale that measure the familiarity between the ATCo with the aircraft. Finally, the solutions consider airspace restrictions such as minimum separation between aircraft and bad weather conditions, i.e., the presence of cumulonimbus (CB). The experiments conducted show that this approach is able to build safe and efficient solution even in situations with a high number of aircraft. / Nos últimos anos, houve um crescimento, no espaço aéreo segregado, nos números do Veículos Aéreos Não-Tripulados (VANT). No entanto, embora exista interesse em integrar grandes VANT em espaço aéreo não-segregado, os desafios de segurança decorrem da inclusão de novas formas de alcançar estados inseguros no espaço aéreo (ATCo) tem como objetivo oferecer níveis adequados de segurança e eficiência e resolver problemas presentes em situações complexas. Embora VANTs possam ser usados em diferentes situações e trazem várias vantagens para o espaço aéreo (por exemplo, eficiência), podem trazer incertezas devido ao fato de que os ATCos não estão familiarizados com essa tecnologia. Ao longo dos anos, esse impacto pode ser menor, e atualmente a falta de familiaridade na relação entre VANT e ATCo contribui para níveis mais altos de carga de trabalho. Além disso, a Área Terminal (TMA), que compõe o espaço aéreo controlado, é uma área de controle crítico normalmente estabelecida na confluência de rotas do Servi¸co de Tráfego Aéreo (ATS), nas quais as aeronaves tendem a estar mais próximas umas das outras. Assim, as operações nesta área particular são realizadas com cuidado e, para alcançar níveis desejáveis de segurança e eficiência, os procedimentos padrão são estabelecidos. Em alguns casos, no entanto, procedimentos padrão não podem ser seguidos e o sequenciamento da aeronave durante a aproximação, que é uma tarefa desafiadora por conta das restrições de manobras complexas, deve ser realizada pelo ATCo de forma a garantir separação mínima entre aeronaves e evitar voos através de cumulonimbus (CB). Finalmente, o principal objetivo de definir um segmento de chegada final ´e entregar o conjunto de aeronaves do setor final, da TMA, para a fase final do seu procedimento de pouso, ou seja, a aproximação final, considerando a eficiência e a segurança da operação. O objetivo desta pesquisa é propor um método paralelo baseado em enxame para otimizar o projeto final de segmentos de chegada de aeronaves, ou seja, rotas que conectem o setor final com o Fixo de Aproximação Inicial (IAF), considerando a presença de VANTs. Esse processo ´e conduzido a partir de duas perspectivas: a carga de trabalho do ATCo, que est´a relacionada à segurança, e a duração da sequenciamento, que está relacionado à eficiência. Além disso, são consideradas diferentes fases da integração de VANTs, ou seja, desde os primeiros estágios de sua integra¸c~ao at´e um estágio maduro de sua operação por meio do uso do Nível de Maturidade Tecnológica (TML), que é uma escala que mede a familiaridade entre o ATCo e a aeronave. Finalmente, as soluções consideram as restrições do espaço aéreo, como a separação mínima entre aeronaves e condições climáticas adversas, isto é, a presença de cumulonimbus (CB). Os experimentos realizados mostram que essa abordagem é capaz de criar soluções seguras e eficientes mesmo em situações com um grande número de aeronaves.

Unmanned aerial systems (UASs) often require multiple human operators fulfilling diverse roles for safe and correct operation. Reliably designing the human interaction, autonomy, and decision making aspects of these systems requires the use of modeling. We propose a conceptual model that models human machine interaction systems as a group of actors connected by a network of communication channels. We present a simulation framework implemented in Java, with an optional XML model parser that can be analyzed using the Java Pathfinder (JPF) model checker. We propose two human workload metrics based on a taxonomy extracted from the relevant literature. Using the simulator to produce a workload profile over time for each human actor, we conducted a case study by modeling a UAS integrated into the National Airspace System. Additionally we adapted an existing cognitive workload metric to act as a baseline. The results of this case study were consistent with known workload events and the results of our baseline metric.

Small Unmanned Aerial Vehicles (SUAVs) are an attractive choice for many surveillance tasks. However, video from an SUAV can be difficult to use in its raw form. In addition, the limitations inherent in the SUAV platform inhibit the distribution of video to remote users. To solve the problems with using SUAV video, we propose a system to automatically create geo-referenced mosiacs of video frames. We also present three novel techniques we have developed to improve ortho-rectification and geo-location accuracy of the mosaics. The most successful of these techniques is able to reduce geo-location error by a factor of 15 with minimal computational overhead. The proposed system overcomes communications limitations by transmitting the mosaics to a central server where there they can easily be accessed by remote users via the Internet. Using flight test results, we show that the proposed mosaicking system achieves real-time performance and produces high-quality and accurately geo-referenced imagery.

Excessive erosion and fine sediment delivery to river corridors and receiving waters degrade aquatic habitat, add to nutrient loading, and impact infrastructure. Understanding the sources and movement of sediment within watersheds is critical for assessing ecosystem health and developing management plans to protect natural and human systems. As our changing climate continues to cause shifts in hydrological regimes (e.g., increased precipitation and streamflow in the northeast U.S.), the development of tools to better understand sediment dynamics takes on even greater importance. In this research, advanced geomatics and machine learning are applied to improve the (1) monitoring of streambank erosion, (2) understanding of event sediment dynamics, and (3) prediction of sediment loading using meteorological data as inputs. Streambank movement is an integral part of geomorphic changes along river corridors and also a significant source of fine sediment to receiving waters. Advances in unmanned aircraft systems (UAS) and photogrammetry provide opportunities for rapid and economical quantification of streambank erosion and deposition at variable scales. We assess the performance of UAS-based photogrammetry to capture streambank topography and quantify bank movement. UAS data were compared to terrestrial laser scanner (TLS) and GPS surveying from Vermont streambank sites that featured a variety of bank conditions and vegetation. Cross-sectional analysis of UAS and TLS data revealed that the UAS reliably captured the bank surface and was able to quantify the net change in bank area where movement occurred. Although it was necessary to consider overhanging bank profiles and vegetation, UAS-based photogrammetry showed significant promise for capturing bank topography and movement at fine resolutions in a flexible and efficient manner. This study also used a new machine-learning tool to improve the analysis of sediment dynamics using three years of high-resolution suspended sediment data collected in the Mad River watershed. A restricted Boltzmann machine (RBM), a type of artificial neural network (ANN), was used to classify individual storm events based on the visual hysteresis patterns present in the suspended sediment-discharge data. The work expanded the classification scheme typically used for hysteresis analysis. The results provided insights into the connectivity and sources of sediment within the Mad River watershed and its tributaries. A recurrent counterpropagation network (rCPN) was also developed to predict suspended sediment discharge at ungauged locations using only local meteorological data as inputs. The rCPN captured the nonlinear relationships between meteorological data and suspended sediment discharge, and outperformed the traditional sediment rating curve approach. The combination of machine-learning tools for analyzing storm-event dynamics and estimating loading at ungauged locations in a river network provides a robust method for estimating sediment production from catchments that informs watershed management.

Intelligence, surveillance, and reconnaissance unmanned aircraft systems (UAS) are the most common variety of UAS in use today and provide invaluable capabilities to both the military and civil services. Keeping the sensors centered on a point of interest for an extended period of time is a demanding task requiring the full attention and cooperation of the UAS pilot and sensor operator. There is great interest in developing technologies which allow an operator to designate a target and allow the aircraft to automatically maneuver and track the designated target without operator intervention. Presently, the barriers to entry for developing these technologies are high: expertise in aircraft dynamics and control as well as in real- time motion video analysis is required and the cost of the systems required to flight test these technologies is prohibitive. However, if the research intent is purely to develop a vehicle maneuvering controller then it is possible to obviate the video analysis problem entirely. This research presents a solution to the target tracking problem which reliably provides automatic target detection and tracking with low expense and computational overhead by making use of the infrared sensor from a Nintendo Wii Remote Controller.

Les considérations environnementales occupent une place croissante dans l'opinion publique ainsi que dans les politiques nationales et internationales. Le cas de la rivière en est un exemple particulièrement frappant : au centre d'enjeux multiples et pour certains hautement stratégiques (énergie, irrigation), elle est le théâtre d'un conflit d'usages donnant lieu à l'établissement de compromis particulièrement fins pour la gestion de la ressource. Dans les approches les plus courantes, la description de l'état et du fonctionnement d'un hydrosystème tel que celui de la rivière est effectuée par la mesure de variables physiques ou la mise en relation des variables d'intérêt avec les paramètres physiques quantifiables caractérisant le milieu (modèles hydrauliques, modèles hydrobiologiques). L'objectif de ce travail est donc de proposer une réponse à la question de la mesure de la géométrie de la rivière par télédétection. On s'attache tout d'abord à décrire le contexte particulier de la rivière mentionné plus haut puis à effectuer un état de l'art faisant ressortir une lacune pour la restitution du relief immergé par stéréo à travers l'eau avec des moyens légers. On propose ensuite une méthode complète d'acquisition d'images et de traitements permettant d'estimer le relief immergé de rivière. Le travail effectué repose sur la mise en oeuvre de moyens technologiques légers permettant une grande flexibilité pour l'acquisition des données. On se base sur les méthodes de stéréorestitution du relief, pour lesquelles on propose une adaptation au contexte spécifique de la photogrammétrie à travers l'eau. Enfin, on met en place des méthodes de correction des mesures brutes intégrant des contraintes a priori (cohérence hydraulique ; cohérence spatiale) pour la correction de l'effet de réfraction. L'application de la méthode développée en conditions opérationnelles sur une rivière peu profonde à fond de galets montre que la mesure du relief immergé par stéréophotogrammétrie est possible, et permet une représentation de la topographie du lit d'une qualité compatible avec les modèles hydrobiologiques (modèles habitat-poisson). On a ainsi développé, testé, validé et determiné les conditions d'application d'une méthode de mesure de la géométrie de la rivière par télédétection originale et complémentaire des technologies existantes.

Soy contains many bioactive molecules known to elicit anti-cancer effects. One such peptide, Lunasin, has been shown to selectively act on newly transformed cells while having no cytotoxic effect on non-tumorigenic or established cancer cell lines. In this study we attempt to understand the developmental effects of Lunasin overexpression in vivo and create reagents that will help us understand Lunasin’s anti tumorigenic effects in an intact organism. cDNA encoding lunasin and EGFP-lunasin were cloned into pUAST and microinjected into Drosophila embryos. Tissue-specific overexpression of EGFP-Lun in the resulting transgenic lines was accomplished by crossing transgenics to various GAL4 driver lines. Progeny were assessed for phenotypic alterations and no phenotypic abnormalities were observed in tissues expressing EGFP-Lunasin, supporting current studies that show Lunasin does not affect normal cells. Previous studies have localized Lunasin to the nuclear compartment. To test if this was the case for EGFP-Lun, subcellular localization of EGFP-Lun was determined via fluorescence microscopy. Salivary glands from EGFP-Lun expressing individuals were dissected, fixed, and mounted in Vectashield® with the nuclear stain, DAPI. Our results demonstrate that EGFP-Lun, like native Lunasin, is localized to the nucleus. Eight transgenic lines were mapped to specific chromosomes and EGFP-Lun transgenic line GEJ1-L2 was balanced in preparation for use in tumor suppression studies. In summary, we have created and characterized transgenic flies capable of overexpressing Lunasin under the control of the GAL4/UAS system. Localization of EGFP-Lunasin to the nucleus and data on the phenotypic consequence of its overexpression in flies is presented. Finally, reagents created as part of this thesis will aid experiments aimed at understanding the effects of Lunasin on benign and invasive tumors.

Unmanned aerial vehicles (UAVs) have been used increasingly over the past few years. Special Forces of various countries utilise these systems successfully in war zones such as Afghanistan. The biggest advantage is rapid information gathering without endangering human lives. The South African National Defence Force (SANDF) also identified the need for local short range aerial reconnaissance and information gathering. A detailed literature survey identified various international players involved in the development of small hand-launch UAV systems. Unfortunately, these overseas systems are too expensive for the SANDF. A new system had to be developed locally to comply with the unique requirements, and budget, of the SANDF. The survey of existing systems provided valuable input to the detailed user requirement statement (URS) for the new South African development. The next step was to build a prototype using off-the-shelf components. Although this aircraft flew and produced good video images, it turned out to be unreliable. The prototype UAV was then replaced with a standard type model aircraft, purchased from Micropilot. Some modifications were needed to ensure better compliance with the URS. Laboratory and field tests proved that the aircraft can be applied for aerial images, within range of 10 km from the ground control station (GCS). The major limitation is that it can only fly for 40 minutes. Furthermore, the airframe is not robust, needing repairs after only 15 flights. Although the system has shortcomings, it has already been used successfully. It is expected that improved battery technologies and sturdier light-weight materials will further help to improve the system beyond user specifications. / Thesis (MIng (Electrical Engineering))--North-West University, Potchefstroom Campus, 2010.