Human activities have dramatically altered the functioning of ecosystems through the ages. Urbanization illustrates the effect of anthropogenic activity by the transformation of natural areas to ecologically disturbed regions (development of towns, cities and settlements). The growing need for urban employment in South Africa has led to an increase in the number of informal settlements on the periphery of urban areas. These settlements result in fragmentation and sprawling of cities, which intensifies strain on the natural environment. Fragmentation in urban regions then leads to the formation of 'patches' of land which exhibit different disturbance levels and are generally typified as either urban, suburban or rural areas. These land use types may be ecologically studied along an urbanization gradient, with the intention of obtaining meaningful comparisons. An urbanization gradient contains an urban landscape which consists of a densely built and developed core surrounded by an area of decreasing development and increasing 'naturalness'. The use of urbanization gradients has been proven world-wide as a useful tool for the study of changes in ecological patterns and processes across landscapes. This approach has been used to examine many different impacts of urbanization, namely on invertebrate communities, bird community composition and plant community composition. Using biological indicators to determine the degree of anthropogenic impact on the environment has proven effective in past studies. These indicators can be used to monitor ecological change following habitat disturbance, identify changing trends over time, provide early warning systems of degradation and diagnose the cause of existing problems. Several authors have supported the use of arthropods as suitable indicators of disturbance. The aim of this study was to determine what impact disturbance, due to urbanization, may have had on the diversity and abundances of epigeal (surface roaming) arthropods (focussing on ants, beetles and spiders) following an urbanization gradient approach. In addition, plant and soil data were combined with the arthropod analysis for each site studied, in order to obtain a better picture of how arthropod community composition would change in relation to these factors. The ant group were the numerically dominant group of the arthropods studied, although the beetles did have the highest number of species captured. Spiders were caught in low abundances, but were also represented by a high number of species. Dramatic decreasing trends were observed with respect to the ant abundances and diversity from rural to the more urbanized sites. Quite the opposite, seemed to occur with the beetles and spiders, who were dominant in species and numbers in the urbanized areas. This trend may be explained on account of the occurrence of generalists and opportunistic beetle and spider species, which seem to thrive in these heterogenous urban habitats. When considering environmental components, percentage bare-ground and sand concentration seemed to be the determining factors in the rural sites, around which the ant group aggregated. Sandy habitats with patches of bare-ground provide more favourable micro-habitats for the ant species to roam and scavenge in, and are advantageous for nest building. Clay concentration and abundance of fruit seemed to assist in providing favourable habitats for the opportunistic and generalist beetle species, in the urbanized areas. High clay concentrations in the urban areas provided ideal conditions for abundant organic covering which would favour saprophagous (feed on decaying organic matter) beetle species and support diverse prey for the predatory beetle and spider species to feed on. Abundance of fruit may have attracted numerous herbivorous beetles (frugivorous beetles). Urbanization seemed to have a more pronounced effect on ant diversity and abundances in comparison to the beetles and spiders, and therefore recommended for future utilization as a suitable "Bio-indicator" group for further local disturbance studies. / Thesis (M. Environmental Science)--North-West University, Potchefstroom Campus, 2008.

Human activities have dramatically altered the functioning of ecosystems through the ages. Urbanization illustrates the effect of anthropogenic activity by the transformation of natural areas to ecologically disturbed regions (development of towns, cities and settlements). The growing need for urban employment in South Africa has led to an increase in the number of informal settlements on the periphery of urban areas. These settlements result in fragmentation and sprawling of cities, which intensifies strain on the natural environment. Fragmentation in urban regions then leads to the formation of 'patches' of land which exhibit different disturbance levels and are generally typified as either urban, suburban or rural areas. These land use types may be ecologically studied along an urbanization gradient, with the intention of obtaining meaningful comparisons. An urbanization gradient contains an urban landscape which consists of a densely built and developed core surrounded by an area of decreasing development and increasing 'naturalness'. The use of urbanization gradients has been proven world-wide as a useful tool for the study of changes in ecological patterns and processes across landscapes. This approach has been used to examine many different impacts of urbanization, namely on invertebrate communities, bird community composition and plant community composition. Using biological indicators to determine the degree of anthropogenic impact on the environment has proven effective in past studies. These indicators can be used to monitor ecological change following habitat disturbance, identify changing trends over time, provide early warning systems of degradation and diagnose the cause of existing problems. Several authors have supported the use of arthropods as suitable indicators of disturbance. The aim of this study was to determine what impact disturbance, due to urbanization, may have had on the diversity and abundances of epigeal (surface roaming) arthropods (focussing on ants, beetles and spiders) following an urbanization gradient approach. In addition, plant and soil data were combined with the arthropod analysis for each site studied, in order to obtain a better picture of how arthropod community composition would change in relation to these factors. The ant group were the numerically dominant group of the arthropods studied, although the beetles did have the highest number of species captured. Spiders were caught in low abundances, but were also represented by a high number of species. Dramatic decreasing trends were observed with respect to the ant abundances and diversity from rural to the more urbanized sites. Quite the opposite, seemed to occur with the beetles and spiders, who were dominant in species and numbers in the urbanized areas. This trend may be explained on account of the occurrence of generalists and opportunistic beetle and spider species, which seem to thrive in these heterogenous urban habitats. When considering environmental components, percentage bare-ground and sand concentration seemed to be the determining factors in the rural sites, around which the ant group aggregated. Sandy habitats with patches of bare-ground provide more favourable micro-habitats for the ant species to roam and scavenge in, and are advantageous for nest building. Clay concentration and abundance of fruit seemed to assist in providing favourable habitats for the opportunistic and generalist beetle species, in the urbanized areas. High clay concentrations in the urban areas provided ideal conditions for abundant organic covering which would favour saprophagous (feed on decaying organic matter) beetle species and support diverse prey for the predatory beetle and spider species to feed on. Abundance of fruit may have attracted numerous herbivorous beetles (frugivorous beetles). Urbanization seemed to have a more pronounced effect on ant diversity and abundances in comparison to the beetles and spiders, and therefore recommended for future utilization as a suitable "Bio-indicator" group for further local disturbance studies. / Thesis (M. Environmental Science)--North-West University, Potchefstroom Campus, 2008.

The diversity of microorganisms and the influence of their enzymatic activities in soil are critical to the maintenance of good soil health. Changes in these parameters may be the earliest predictors of soil quality changes, potentially indicating anthropogenic influences. The goal of this study was to investigate the soil microbial diversity and function of grasslands along an urbanization gradient. Soil samples were collected in the Potchefstroom municipal area, South Africa, at specific sites. Sampling sites were described as urban, suburban and rural - according to the V-I-S (Vegetation-Impervious surface-Soil) model of Ridd (1995). Soil samples were collected over a warmer, wet season (May) and a colder, dry season (August) over two years (2007 and 2008). Collected soil samples were characterised using certain physical and chemical parameters. Plant species composition and abundance were determined at each site, along with basic site data (soil compaction, percentage ground cover, percentage bare ground, percentage organic material present). The Shannon-Weaver diversity index was used to calculate biodiversity values for all the investigated sites regarding collected plant species composition. The microbial component of the soil was quantified and characterized using culture-dependent and culture-independent techniques. Culture-dependent techniques included the investigation of the aerobic heterotrophic bacteria and fungi. Organisms were plated out on different media, and the bacterial component was broadly grouped using morphology. Dominant organisms were identified by sequencing of PCR amplified 16S ribosomal DNA fragments. Shannon-Weaver index for bacterial diversity was determined for each of the sites. Denaturing gradient gel electrophoresis (DGGE) profiling of selected bacterial communities were also conducted. Microbial community function was determined using enzyme assays of five major groups of enzymes, namely (i) dehydrogenase; (ii) β-glucosidase; (iii) acid phosphatase, (iv) alkaline phosphatase and (v) urease. Plant species results were then brought into context with microbiological diversity and functionality results using multivariate statistics. Physical and chemical parameters of the collected soil samples revealed patterns present along the urbanization gradient. The pH values were mostly higher in the sub-urban and urban sites than in the rural sites. Electrical conductivity values were generally highest in the sub-urban sites. Plant species composition revealed trends along the urbanization gradient. Ordinations clearly grouped the plant species into rural, sub-urban and urban groups regarding plant species composition. Rural sites had the highest number of plant species. Shannon-Weaver values regarding the plant diversity supported the plant species composition data indicating higher plant diversity in the rural areas, followed by the sub-urban and the urban areas. Plant structural data indicated that forbs were most numerous in the rural sites, and less so in the urban sites. Higher average aerobic heterotrophic bacterial levels were present in the urban soil samples. The bacterial levels were lower in the sub-urban and rural soil samples. Subsequent identification of the dominant bacteria in the soil samples revealed organisms of the genus Bacillus dominated the aerobic heterotrophic bacterial communities in the soil samples. Bacillus species dominated the soil samples along the urbanization gradient. Shannon-Weaver indices based on culture-dependent methods indicated that urban sites had the highest biodiversity. These results could have been exaggerated, because of an overestimation of the number of bacterial morphotypes present in samples. Fungal levels were higher in the soil from samples collected at the rural samples sites. The culture-independent method (DGGE) was not optimized and inconclusive results were obtained. Enzyme assays revealed that potential dehydrogenase, β-glucosidase and urease activity followed a trend along the urbanization gradient, with urban samples registering the highest values and rural sites the lowest. Enzymes involved in carbohydrate catabolism (β-glucosidase and dehydrogenase) registered significantly higher potential activity in urban sites than the sub-urban and rural sites. The results could indicate that urban sites have the potential to lose carbon at higher rates than the rural sites. This aspect may need further investigation. Higher potential urease activity could indicate higher N-cycling in the urban soil environment. Ordination results for soil-, plant- and microbial diversity as well as microbial functionality indicated certain trends along the urbanization gradient. Plant species composition and structure data indicated that urbanization has a definite effect on the plant communities in the urban ecosystem. Results regarding aerobic heterotrophic bacteria populations and potential enzyme activity of the dehydrogenase, β-glucosidase (both active in the carbon cycle) and urease (active in the nitrogen cycle) illustrated clear trends along the urbanization gradient. In conclusion, results indicated that urbanization has an effect on plant species composition, and the population and function of aerobic heterotrophic bacteria and the fungal population. Furthermore, this study demonstrated the potential of using microbial diversity and activity as tools to investigate carbon utilization and storage along an urban-rural gradient. / MSc (Environmental Sciences), North-West University, Potchefstroom Campus, 2011

The diversity of microorganisms and the influence of their enzymatic activities in soil are critical to the maintenance of good soil health. Changes in these parameters may be the earliest predictors of soil quality changes, potentially indicating anthropogenic influences. The goal of this study was to investigate the soil microbial diversity and function of grasslands along an urbanization gradient. Soil samples were collected in the Potchefstroom municipal area, South Africa, at specific sites. Sampling sites were described as urban, suburban and rural - according to the V-I-S (Vegetation-Impervious surface-Soil) model of Ridd (1995). Soil samples were collected over a warmer, wet season (May) and a colder, dry season (August) over two years (2007 and 2008). Collected soil samples were characterised using certain physical and chemical parameters. Plant species composition and abundance were determined at each site, along with basic site data (soil compaction, percentage ground cover, percentage bare ground, percentage organic material present). The Shannon-Weaver diversity index was used to calculate biodiversity values for all the investigated sites regarding collected plant species composition. The microbial component of the soil was quantified and characterized using culture-dependent and culture-independent techniques. Culture-dependent techniques included the investigation of the aerobic heterotrophic bacteria and fungi. Organisms were plated out on different media, and the bacterial component was broadly grouped using morphology. Dominant organisms were identified by sequencing of PCR amplified 16S ribosomal DNA fragments. Shannon-Weaver index for bacterial diversity was determined for each of the sites. Denaturing gradient gel electrophoresis (DGGE) profiling of selected bacterial communities were also conducted. Microbial community function was determined using enzyme assays of five major groups of enzymes, namely (i) dehydrogenase; (ii) β-glucosidase; (iii) acid phosphatase, (iv) alkaline phosphatase and (v) urease. Plant species results were then brought into context with microbiological diversity and functionality results using multivariate statistics. Physical and chemical parameters of the collected soil samples revealed patterns present along the urbanization gradient. The pH values were mostly higher in the sub-urban and urban sites than in the rural sites. Electrical conductivity values were generally highest in the sub-urban sites. Plant species composition revealed trends along the urbanization gradient. Ordinations clearly grouped the plant species into rural, sub-urban and urban groups regarding plant species composition. Rural sites had the highest number of plant species. Shannon-Weaver values regarding the plant diversity supported the plant species composition data indicating higher plant diversity in the rural areas, followed by the sub-urban and the urban areas. Plant structural data indicated that forbs were most numerous in the rural sites, and less so in the urban sites. Higher average aerobic heterotrophic bacterial levels were present in the urban soil samples. The bacterial levels were lower in the sub-urban and rural soil samples. Subsequent identification of the dominant bacteria in the soil samples revealed organisms of the genus Bacillus dominated the aerobic heterotrophic bacterial communities in the soil samples. Bacillus species dominated the soil samples along the urbanization gradient. Shannon-Weaver indices based on culture-dependent methods indicated that urban sites had the highest biodiversity. These results could have been exaggerated, because of an overestimation of the number of bacterial morphotypes present in samples. Fungal levels were higher in the soil from samples collected at the rural samples sites. The culture-independent method (DGGE) was not optimized and inconclusive results were obtained. Enzyme assays revealed that potential dehydrogenase, β-glucosidase and urease activity followed a trend along the urbanization gradient, with urban samples registering the highest values and rural sites the lowest. Enzymes involved in carbohydrate catabolism (β-glucosidase and dehydrogenase) registered significantly higher potential activity in urban sites than the sub-urban and rural sites. The results could indicate that urban sites have the potential to lose carbon at higher rates than the rural sites. This aspect may need further investigation. Higher potential urease activity could indicate higher N-cycling in the urban soil environment. Ordination results for soil-, plant- and microbial diversity as well as microbial functionality indicated certain trends along the urbanization gradient. Plant species composition and structure data indicated that urbanization has a definite effect on the plant communities in the urban ecosystem. Results regarding aerobic heterotrophic bacteria populations and potential enzyme activity of the dehydrogenase, β-glucosidase (both active in the carbon cycle) and urease (active in the nitrogen cycle) illustrated clear trends along the urbanization gradient. In conclusion, results indicated that urbanization has an effect on plant species composition, and the population and function of aerobic heterotrophic bacteria and the fungal population. Furthermore, this study demonstrated the potential of using microbial diversity and activity as tools to investigate carbon utilization and storage along an urban-rural gradient. / MSc (Environmental Sciences), North-West University, Potchefstroom Campus, 2011

Urban open spaces are of strategic importance to the quality of life of our increasingly urbanized society. Trees and related vegetation are planted and managed within the communities and cities to create or add value to the busy lives of the city dwellers. Trees in towns and cities form an important part of complex urban ecosystems and provide significant ecosystem services and benefits for urban dwellers, for example: reducing particulate pollution, carbon sequestration, decreasing air temperature, decreasing water runoff, aesthetic value and an increase in human health. Trees are solarpowered technology that can help restore balance to dysfunctional urban ecosystems. Trees form strands in the urban fabric that connect people to nature and to each other. The urban environment puts tremendous strain on trees by trenching, limited space for root growth and emission of pollutants into the atmosphere, water and soil. The problem is that the real impact of the urban environment on the trees within our community is unknown. The aim of this investigation was to assess the overall anthropogenic and environmental impacts on urban trees by measuring the tree vitality of Acacia karroo using chlorophyll fluorescence kinetics (JIP-test) and the leaf water potential using a pressure chamber. Tree vitality was quantified as the chlorophyll fluorescence-based performance index (PIABS)T. ree vitality measurements were also correlated with soil physical and chemical data. In the comparative study, an urbanization gradient approach was followed in which results of trees in rural areas were regarded as controls. The gradient approach is used worldwide and provides a background for questions of ecological structure and function. The urbanization gradient was quantified using the V-I-S model, based on % cover of vegetation, impervious surface and soil. Additionally, a model to determine the monetary value of trees in urban environments (SATAM) was tested. All this information could eventually contribute to develop an urban tree management program for Potchefstroom. It was evident from the current study that urbanization has a negative impact on tree vitality. The leaf water potential of a tree was, however, not necessarily negatively impacted upon. Although trees in urban environments did not always have a high vitality (PIABS)t, hey still played a major role in the urban environment. According to the tree appraisal method (SATAM), some of these trees have a value of R60 000. / Thesis (M. Environmental Science (Ecological Remediation and Sustainable Utilisation))--North-West University, Potchefstroom Campus, 2007.

Thesis (M. Environmental Science (Ecological Remediation and Sustainable Utilisation))--North-West University, Potchefstroom Campus, 2007.

Urban open spaces are of strategic importance to the quality of life of our increasingly urbanized society. Trees and related vegetation are planted and managed within the communities and cities to create or add value to the busy lives of the city dwellers. Trees in towns and cities form an important part of complex urban ecosystems and provide significant ecosystem services and benefits for urban dwellers, for example: reducing particulate pollution, carbon sequestration, decreasing air temperature, decreasing water runoff, aesthetic value and an increase in human health. Trees are solarpowered technology that can help restore balance to dysfunctional urban ecosystems. Trees form strands in the urban fabric that connect people to nature and to each other. The urban environment puts tremendous strain on trees by trenching, limited space for root growth and emission of pollutants into the atmosphere, water and soil. The problem is that the real impact of the urban environment on the trees within our community is unknown. The aim of this investigation was to assess the overall anthropogenic and environmental impacts on urban trees by measuring the tree vitality of Acacia karroo using chlorophyll fluorescence kinetics (JIP-test) and the leaf water potential using a pressure chamber. Tree vitality was quantified as the chlorophyll fluorescence-based performance index (PIABS)T. ree vitality measurements were also correlated with soil physical and chemical data. In the comparative study, an urbanization gradient approach was followed in which results of trees in rural areas were regarded as controls. The gradient approach is used worldwide and provides a background for questions of ecological structure and function. The urbanization gradient was quantified using the V-I-S model, based on % cover of vegetation, impervious surface and soil. Additionally, a model to determine the monetary value of trees in urban environments (SATAM) was tested. All this information could eventually contribute to develop an urban tree management program for Potchefstroom. It was evident from the current study that urbanization has a negative impact on tree vitality. The leaf water potential of a tree was, however, not necessarily negatively impacted upon. Although trees in urban environments did not always have a high vitality (PIABS)t, hey still played a major role in the urban environment. According to the tree appraisal method (SATAM), some of these trees have a value of R60 000. / Thesis (M. Environmental Science (Ecological Remediation and Sustainable Utilisation))--North-West University, Potchefstroom Campus, 2007.

No description available.

L'urbanisation croissante de ces dernières décennies nécessite une connaissance approfondie de la dynamique de la biodiversité en milieu urbain afin d’aider à la mise en place de politiques de développement urbain favorisant le maintien de cette biodiversité. Ce travail de doctorat a évalué l'homogénéisation taxonomique et la composition fonctionnelle des communautés de flore spontanée le long d’un gradient d’urbanisation en prenant comme unité d’étude le jardin privatif, ainsi que la structure génétique d’une espèce de plante, Parietaria judaica.L'homogénéisation taxonomique varie le long du gradient d’urbanisation. Les jardins privatifs des centres villes étudiés seraient moins riches, mais favoriseraient la diversité taxonomique au regard de la composition spécifique des communautés de par leurs expositions, aménagements et pratiques. L'étude des traits des communautés met en évidence des variations de la composition fonctionnelle le long du gradient d’urbanisation. Les zones de forte densité de bâti filtrent des traits, notamment liés à la dispersion et à la persistance des espèces. L'endozoochorie pourrait permettre le franchissement des bâtis qui entourent les jardins.Enfin, l’absence de structure génétique de Parietaria judaica à l’échelle locale des villes suggère des échanges génétiques quelle que soit la densité de bâti, tandis qu’une structure génétique marquée est observée à l’échelle régionale, entre les trois sites.Le jardin privatif qui à l’instar des autres habitats du milieu urbain, présentent des communautés végétales qui peuvent participer aux dynamiques de la végétation urbaine. / Increasing urbanization in recent decades requires a thorough understanding of the dynamics of urban biodiversity to aid in the establishing of urban development policies to promote the biodiversity. This doctoral work evaluated the taxonomic homogenization and functional composition of spontaneous flora communities along a gradient of urbanization taking as the unit of study the private garden, and the genetic structure of a plant species, Parietaria judaica.Taxonomic homogenization varies along the urbanization gradient. The private gardens of the centers studied cities are less rich but would enhance taxonomic diversity in terms of species composition of communities through their exposure, planning and practices.The study of traits communities highlights changes in the functional composition along the urbanization gradient. High built-up density areas filter traits, in particular linked to the dispersion and persistence of species. The endozoochorie could allow the crossing of built around the gardens.Finally, the lack of genetic structure of Parietaria judaica at local scale suggests genetic exchange regardless of the built-up density, while a significant genetic structure was observed at the regional level between the three sites.The private gardens, which like the other green patchs in urban areas, have plant communities that can participate in the dynamics of urban vegetation.

L'écologie urbaine, qui a émergé depuis une trentaine d'années, s'intéresse notamment à l'étude de la biodiversité et du fonctionnement des écosystèmes en ville. Ce travail de thèse porte plus particulièrement sur l'analyse de l'écosystème "corridor fluvial", et son rôle dans la dynamique des communautés végétales en fonction d'un gradient d'urbanisation. Les corridors fluviaux assurent en effet une connexion structurelle entre la ville et la campagne, et leur conservation en milieu urbain implique d'en comprendre précisément la fonctionnalité écologique. L'objectif de ce travail est donc de mettre en évidence l'effet du gradient d'urbanisation sur la composition et la structure des communautés végétales le long d'un cour d'eau et de vérifier si l'existence en ville de corridors fluviaux contribue efficacement à la dispersion et à la rapidité de colonisation des milieux adjacents. Deux cours d'eau, la Bruche et l'hydrosystème Rhin Tortu - Ziegelwasser, ont été étudiés dans leur partie aval la plus urbaine au sein de l'Eurométropole de Strasbourg. [...] / Urban ecology, which has emerged over the last thirty years, focused in particular on studying thebiodiversity and on functioning of ecosystems in cities. This work explores urban riparian corridors and their rolein the dynamic of plant communities based on a gradient of urbanization. Indeed, riparian corridors provide astructural connection between the city and the countryside, and their conservation involves preciselyunderstanding their ecological functionality. The objectives of this work are to highlight the effect of the urbanization gradient on the composition and structure of plant communities along a river to determine whether the existence of urban riparian corridors effectivelycontributes to the colonization of adjacent areas. Two rivers, the Bruche and the Rhin Tortu - Ziegelwasser, arestudied in their most urban downstream part in the metropolitan area of Strasbourg, eastern France. [...]