I studied a small, enigmatic, and imperiled black bear population in south-central Florida from 2004 - 2006. Annual home ranges of males (96.0 km2) were larger than those of females (32.2 km2). Female home ranges were smaller in winter than in summer or fall. At the landscape scale, bears selected forests, scrub, and citrus, but avoided urban areas. At the home range scale, bears selected bay swamp and hardwood hammock, but avoided urban areas and grassland. Bears selected bay swamp in winter, forests and scrub in summer, and forests, scrub, and marsh in fall. The bear’s diverse diet included citrus fruit. Important foods were acorn, saw palmetto fruit, and Florida carpenter ant. The local landscape is dominated by agriculture on private lands, as opposed to large contiguous forests on public land elsewhere in Florida black bear range. Mean patch size of forests was smaller, while edge density, diversity, and evenness were higher in south-central Florida than elsewhere in the state. Diversity of forest habitat may partially account for the persistence of the black bear in this fragmented landscape. Managers should encourage private landowners to adopt practices that promote bear habitat, and focus on habitat diversity, road crossings, and statewide metapopulation structure.

Wildlife managers use models to aid in predicting high risk areas for human and black bear (Ursus americanus) interactions (HBI). These tools help managers implement management strategies to minimize HBI. Over 3,000 incidents of HBI were compiled from management reports at Great Smoky Mountains National Park (GSMNP) during 1998-2011, a park with 9-10.2 million visitors per year and a black bear population of about 1,600 bears. We used data from bear management reports along with annual visitor use, mast and bear abundance data to develop a series of generalized linear models to assess the spatial and temporal factors affecting HBI. Although HBI occurred throughout the GSMNP, 50% of all HBI occurred in five areas. The best predictor variables of HBI across four subsets of models included interaction between mast production and number of park visitors, month, vegetation cover, visitor activity, and bear abundance. Although there was not a clear relationship between visitor use and mast abundance, the number of park visitors was always relatively high and HBI increased substantially in poor mast years. HBI was more frequent during summer months when park visitation rates and more people and food were present overnight in frontcountry and backcountry camping areas. Over 43% of HBI in hemlock forests were serious. Bear abundance data were not a strong predictor of HBI, and bear bait stations may not provide a sensitive index to bear abundance. GSMNP uses different strategies for managing HBI to protect visitors and bears. In 1991, bear proof waste disposal containers and food storage devices were placed in camping and picnic areas. In combination with aversive conditioning, HBI decreased in some areas of the park. We recommend that proactive bear management programs including education, enforcement of park regulations, and aggressive aversion conditioning of bears be implemented at the identified HBI high risk areas to provide a safer environment for both people and bears in GSMNP.

Understanding species’ distribution, density, and sources of bias in population estimates is critical for reliable conservation strategies. I assessed American black bear distribution, density, and abundance in southern Missouri. Using anecdotal occurrence data, I demonstrated support for a northward trend in extent of occurrences over time and a positive correlation between bear distribution and human–bear incidents. I also used GPS telemetry and camera traps to investigate detection biases in DNA hair snare methods and tested efficacy of two sampling designs for estimating density using spatial capture-recapture models. Results demonstrated that detection probability decreased following a negative asymptotic relationship with decreasing bear proximity to snares and that hair deposition rates decreased over time. Precision of estimates for low density populations with non-uniform distribution increased when using multiple arrays with intensive snare spacing. Optimizing the tradeoff among snare spacing, coverage, and sample size is important for estimating parameters with high precision.

Dens are a critical component of black bear (Ursus americanus) habitat, yet scale-dependent den-site selection has received limited attention. Natural and anthropogenic factors (e.g., vegetation, roads) may also influence bear den-site selection. I quantified black bear denning chronology and den use and evaluated multi-scale den-site selection in Mississippi, USA during 2005–2011. Females entered dens earlier than males and emerged later; multiple den use by both sexes in a single winter was common. I recorded equal numbers of tree and ground dens, with ground dens at higher elevations surrounded by dense vegetation. Chronology and other denning characteristics of bears in Mississippi were similar to other black bear populations in the southeastern United States. Bears exhibited scale-dependent den-site selection selecting sites with greater percentage horizontal cover and farther from roads. Greater percentage horizontal cover may provide security and increase energetic efficiency. Denning farther from roads likely decreases risk of human disturbance.

The population trajectory of woodland caribou (Rangifer tarandus caribou) in Newfoundland is currently determined by low calf survival due to high predation rates during the first 6-8 weeks after parturition. Most caribou in Newfoundland congregate and give birth in open calving grounds; consequently, in order to investigate predator-prey interactions, design research, and develop mitigation strategies, the geographic extent of the caribou calving grounds must be properly identified. We used VHF telemetry locations of caribou calves, collected from 2003-2010, to determine the spatial and temporal extent of caribou calving grounds in three study areas in Newfoundland. We put GPS collars on 47 black bears (Ursus americanus) in 3 caribou ranges where bears are having a significant impact on caribou recruitment by preying on calves during the calving season. Bear movements were greatest during the calving season, potentially increasing encounters with calves. Some bears migrated to the calving grounds just prior to caribou parturition, indicating deliberate broad-scale selection of areas of high calf density. Bears displayed interannual fidelity to calving ground usage patterns during the calving season, with some bears using the calving grounds every year, while others did not. We estimated the probability of a bear spending time in the calving grounds during the calving season as a function of the bear’s sex and mean distance to the calving grounds with logistic regression. We found that as distance increased, the odds of a bear spending time in the calving grounds decreased, and that at any given distance the odds were greater for male bears than for female bears. Our results indicate that some bears in Newfoundland are likely caribou calf predators, while others are not, and that the sex and broad-scale distribution of bears influenced the probability of a bear participating in calf predation during the calving season. The probability distribution of calf-visiting bears could be used to develop management practices to mitigate the impact of bear predation on declining caribou herds in Newfoundland.

For years, bear hunters have provided an unknown amount of food to black bears <i>(Ursus americanus)</i> in Virginia, supplementing their natural food supply. Possible effects of feeding bears can be negative, such as food conditioning and habituation to people, or positive, such as enhanced or sustained reproductive rates, especially in years of mast crop failure. In July 1999, the Virginia Department of Game and Inland Fisheries (VDGIF) adopted a regulation recommendation that prohibited the feeding of wildlife on national forest and department-owned lands. We mailed a survey to all members of the Virginia Bear Hunters Association (VBHA, n = 459) to determine the amount of food provided to bears by bear hunters in Virginia between 1 July 1998 and 30 June 1999. Survey response rate was 52%. One hundred thirteen of 238 (47.5%) survey respondents spent over $18,000 on supplemental food in one year, averaging $163/person. One hundred twenty-eight respondents reported cumulatively providing nearly 3 million kilograms of food to bears between 1 July 1998 and 30 June 1999. Whole-shelled corn, pastries, and grease accounted for 58% of the total food by weight; however, whole-shelled corn, pastries, and bread were the 3 most common foods offered. Feeding occurred in 25 counties in Virginia, primarily during July, August, and September. Rockingham County had the highest proportion of feeders (47%), followed by Giles (15%), Augusta (14%), Craig (9%) and Montgomery (9%) Counties. Accordingly, Rockingham County received the highest proportion of food (42%), followed by Craig (8%), Giles (7%), Montgomery (6%), and Tazewell (6%) Counties. The low survey response rate, coupled with the fact that 48% of sampled nonrespondents fed bears, suggests that the total amount of annual feeding in Virginia may exceed the total determined during this study. However, the possibility of an avidity bias, in which a higher response by the more avid feeders would erroneously inflate a total estimate extrapolated to include feeding by nonrespondents, cannot be eliminated. Little work has been done pertaining to digestibilities of black bear foods, particularly those in the eastern United States. We determined digestibilities for several important eastern black bear foods, including northern red oak <i>(Quercus rubra)</i>, white oak <i>(Q. alba)</i>, and chestnut oak acorns <i>(Q. prinus)</i>, squawroot <i>(Conopholis americana)</i>, high-protein dogfood, shelled corn, and doughnuts. All diets were evaluated for nutrient content as well as percent dry matter digestibility of crude protein, ether extract (a measure of fat), and gross energy. Diets we suspected were high in fiber were additionally evaluated for neutral detergent fiber or acid detergent fiber content and digestibility. Feeding trials were conducted with 1 male and 4 female captive bears during fall 1998, and 5 female captive bears during fall 1999, at the Center for Ursid Research, Blacksburg, Virginia. Acorns and squawroot were high in fiber (45-62%) and moderate to low in protein (5-7%). Fat content was high in doughnuts (24.7) and northern red oak acorns (12%), moderate in dogfood (8.2%) and white oak acorns (4.7%), and relatively low in shelled corn, chestnut oak acorns, and squawroot (1.3-3.5%). Crude protein was high in dogfood (29%) and moderate in all other diets (4.7-8.8%). Neutral detergent fiber content was high in squawroot and all acorn types (45-62.4%). Acid detergent fiber was high in squawroot (47%) and moderate in dogfood (9%). In 1998, crude protein digestibility was high in doughnuts (86%), moderate in shelled corn and white oak acorns (50-64%), and negligible in northern red oak acorns (-39%). Ether extract digestibility was high in doughnuts, red oak acorns, and white oak acorns (80-97%), and moderate in shelled corn (67%). Fiber digestibility was tested only on northern red oak and white oak acorns, and was moderate (62% and 68%, respectively). In 1999, crude protein digestibility was high in dogfood, dogfood mixed with squawroot, and doughnuts (75-85%); moderate in shelled corn, squawroot, and white oak acorns (43-58%); and low to negligible in chestnut oak and northern red oak acorns (-13-6%). Ether extract digestibility was moderate in shelled corn (64%) and squawroot (60%), and high in all other diets (79-97%). Neutral detergent fiber digestibility was moderately high in all acorn types (54-71%). Acid detergent fiber digestibility was moderate for dogfood (66%), and associative effects occurred between the fiber in squawroot and dogfood, resulting in negative fiber digestibility in squawroot determined by difference (-19%). A luxury not always realized in wildlife management is the ability to investigate possible effects of management or policy changes prior to their employment. The amount of food provided to bears by bear hunters in Virginia may have been more substantial than previously believed, and likely provided bears with a high energy, stable food source that supplemented their natural food supply. We did not have the opportunity to study the effects of removing that food source on public lands prior to the regulation change; however, the opportunistic feeding strategy of bears, coupled with sufficient mast production at the time of the regulation change, likely softened any negative effects that may have occurred as a result of the removal of supplemental food as a regular food source for some bears. / Master of Science

Thirty-four (6 M, 28 F) of 93 black bears (<i>Ursus americanus</i>) captured during summers 1995 and 1996 were equipped with radio-collars. The mean age of male and females captured was 2.5 (<u>n</u> = 63; 2 males not aged) and 4.4 (<u>n</u> = 28) years, respectively. The mean date of females in estrus was 24 July, and we observed one 1.5-year old female in estrus. The average age of primiparity of radio-collared females was 3.0 years; however, we documented fetuses present in a 2-year old noncollared female's reproductive tract. The average interbirth interval was 1.6 years and 95.4% of females without yearlings produced cubs. The mean litter size was 2.2 and the cub sex ratio was 1.3M:1F. Hunting, vehicle collisions, poaching, research, and euthanasia accounted for 80.5%, 5.5%, 5.5%, 5.5%, and 2.8%, respectively of the adult and juvenile male mortalities (<u>n</u> = 36). Hunting, vehicle collisions, and research each accounted for 2 of the adult and juvenile female mortalities (<u>n</u> = 6). Annual harvest rates for males in 1995 and 1996 were 36.1% and 45.5%, respectively; corresponding harvest rates for females were 0.0%, and 5.9%. Annual survival rates estimated with Kaplan-Meier for adult females, juvenile females, and cubs were 100.0%, 93.3%, and 70.3%, respectively. Maximum juvenile male survival rates were 52.0% in 1995 and 51.7% in 1996. Maximum adult male survival rates were 50.0% and 80.0% in 1995 and 1996, respectively. We monitored 31 bears for 39 bear winters with 100% of the known bears denning. Bears denned in trees (41%), rock cavities (32%), excavations (14%), snags (8%), and ground nests (5%). Chestnut oak (<i>Quercus prinus</i>; <u>n</u> = 9), red oak (<i>Q. rubra</i>; <u>n</u> = 8), and tulip-poplar (<i>Liriodendron tulipifera</i>; <u>n</u> = 1) were used as tree dens. Habitat characteristics did not differ between ground dens and tree dens; however, older bears used ground dens more frequently (<u>Z</u> = -2.484, <u>P</u> = 0.013) than tree dens. Fifty-seven percent of bears denned on public land, and we documented one instance of den reuse. / Master of Science

The United States Fish and Wildlife Service (USFWS) expressed the need to develop appropriate management strategies for apparently high-density, growing black bear populations in the Roanoke-Neuse-Tar-Cape Fear ecosystem in coastal North Carolina and Virginia. In order to provide the scientific information necessary to develop these strategies, I investigated population densities and genetic structure of black bears at 3 national wildlife refuges [Great Dismal Swamp (GDSNWR), Pocosin Lakes (PLNWR), and Alligator River (ARNWR)]. Density estimates were derived from DNA samples collected noninvasively at each of the 3 refuges for 2 consecutive summers. Hair samples were analyzed for individual identification using 6-7 microsatellite markers. Estimated densities were some of the highest reported in the literature and ranged from 0.56-0.63 bears/km2 at GDSNWR to 0.65-1.12 bears/km2 at ARNWR to 1.23-1.66 bears/km2 at PLNWR. Sex ratios were male-biased in all areas of all refuges. Genetic variability and structure of bears at these refuges was assessed using 16 microsatellite markers for 40 bears from each refuge. Genetic variability of the 3 refuge populations was substantially high compared to other bear populations in North America, with observed heterozygosities ranging from 0.6729 at GDSNWR to 0.7219 at ARNWR. FST and DS values were relatively low (0.0257-0.0895 and 0.0971-0.3640, respectively), indicating movement of bears and gene flow across the landscape is adequate to prevent high levels of genetic differentiation and structure among the refuge bears. Genetic statistics at GDSNWR indicate that this population is isolated to some degree by geography (i.e., the Albemarle Sound) and encroaching urban development (i.e., the towns of Suffolk and Chesapeake). ARNWR has the potential to become isolated in the future if movement corridors to the south of the refuge are not maintained. Harvest of bears is likely warranted at PLNWR and ARNWR, though extreme caution must be taken the first few seasons as hunter success will be extremely high. Further research is needed to determine population growth rates, reproductive parameters, and survival rates at all 3 refuges, particularly if a hunting season will be established and maintained in these areas. Methods for regularly monitoring bear populations at these refuges also should be incorporated into biological programs, as bears comprise a significant component of the ecosystem at these refuges and cannot be ignored when outlining management goals. / Master of Science

During 1994-1996, reproductive biology and denning ecology of black bears (<i>Ursus americanus</i>) were investigated in the George Washington and Jefferson National Forests, Virginia. The average age of captured bears was 4.88 years (SE=0.43, n=64) and 2.72 years (SE=0.19, n=127) for females and males, respectively. Timing of estrus ranged from 21 June to 7 August with most (58.8%, n=10) estrus females captured between 15 July and 31 July. Ages of primiparity were 3 or 4 years and averaged 3.14 years (SE=0.14, n=7). Average litter size was 2.0 cubs per litter (n=26). During 1995 and 1996, respectively, 81.8% and 83.3% of potentially pregnant females reproduced. Date of parturition averaged 22 January in 1995 and 1996, but ranged from 1 January to 22 February in 1995 and 11 January to 14 February in 1996. Pregnant females did not enter dens earlier (T=0.74, P=0.4598) than other bears, and did not emerge later (T=-1.69, P=0.0925) than other bears. cavities in trees and snags composed 82.4% (n=56) of all dens. Red oak (<i>Quercus rubra</i>) and chestnut oak (<i>Quercus prinus</i>) were the most commonly (n=49) used den tree species. Tree dens were on sites with steeper slopes (t=3.41, P=0.0013), lower medium tree density (t=-2.22, P=0.0313), higher large tree density (t=2.24, P=0.0296), and lower stem density (t=-3.43, P=0.0013) than random sites. Ground dens were on steeper slopes t=3. 22, P=O. 0122) than random sites. Den reuse occurred at 2 of 17 (11.8%) dens, and 84.6% (11 of 13) of bears, monitored both winters, used the same den type during both winters. Virginia's black bear harvest during 1982-1994 was used to reconstruct Virginia's bear population. During 1982-1991, Virginia I s reconstructed male black bear population ranged from 880 bears to 1,241 bears. Numbers of males increased during 1982-1986, declined during 1987-1989, and increased during 1990-1991. Virginia's reconstructed female black bear population ranged from 943 bears to 1064 bears and increased most years. / Master of Science

The American black bear (ABB) (Ursus americanus) exhibits physiological strategies highly synchronized with the environment. Such strategies enable bears to exploit food resources when available and survive the winter months by hibernating without ingesting food or water. However, there are multiple aspects of ABB hibernation physiology that remain unknown. For instance, there is conflicting evidence on the occurrence of ABB pseudopregnancy (a physiological state in which a non-pregnant bear exhibits progesterone levels similar to gravid bears in the absence of an actual pregnancy). Also, there is little known about postnatal development of cubs or the influence of maternal traits on embryonic implantation and cub growth. Finally, the role of satellite cells (SCs – stem cells able to regenerate muscle fibers) play in maintaining muscle functionality during hibernating remains understudied. Therefore, I aimed to assess these four aforementioned aspects using wild ABBs held temporarily captive at Virginia Tech's Black Bear Research Center (VT-BBRC). The major findings of this dissertation are: 1) I suggest that wild ABBs do not experience pseudopregnancy as a reproductive strategy; 2) interactions between litter size and cub age best described postnatal cub weight dynamics and organ development. Twin cubs were heavier than single and triplet cubs, yet cubs from all litter sizes reached similar weights after mothers began consuming food post hibernation. Single cubs experienced delayed timing in ear, eye, and teeth development compared to other litter sizes; 3) maternal traits such as higher body weight and higher ability to gain weight in the fall are closely associated with earlier timing of embryonic implantation than in leaner females, which gained less weight per day in the fall; and 4) SC ability to generate muscle fibers is increased during ABB hibernation. I propose that maintaining the SCs are an important potential pathway for limiting muscle atrophy during bear hibernation. Understanding pre and postnatal development of ABBs is important for exploring factors related to climate, maternal characteristics, which possibly affect birthing phenology, and fitness of bears experiencing rapid anthropogenic environmental change. Functional aspects of bear muscle conservation are interesting for potentially for elucidating avenues to improve treatments for human metabolic disorders such as muscular dystrophy, sarcopenia, and disuse atrophy. / Ph. D.