Includes bibliographical references.

Bibliography: leaves 155-182. Most agricultural crop seeds are 'orthodox' ie. desiccation is a necessary feature of their complete life cycle. Low moisture content lengthens the storage life of orthodox seeds. Recalcitrant seeds, which do not tolerate low moisture content, lose viability in dry storage. The thesis studies the role of sugars in desiccation tolerance in developing seeds and investigates other mechanisms which might be involved in desiccation tolerance and desiccation sensitivity.

The City of Johannesburg is facing the simultaneous challenges of an increased generation of solid waste in the City, unemployment and the running out of land to dispose the waste. Of the solid waste that is generated, 50% is recyclable while only 10% is recycled (SOER, 2003: 69). Solid waste recycling is mainly performed through private sector initiatives in the City of Johannesburg. Consequently, there is no proper coordination with government initiatives. In order for South Africa to increase and sustain economic growth that would decrease unemployment, there should be a culture of entrepreneurship (Mass, G & Herrington M, 2006:7). Solid waste buy-back centres assist in addressing the challenges of dealing with the increased generation of solid waste and the scarcity of land for disposal. These centres also reduce the challenges of unemployment through promoting entrepreneurs to operate solid waste recycling businesses. Developed economies have managed to increase the rate of solid waste recycling to 60%. The problems associated with solid waste have been reduced by promoting recycling through a combination of legislation and setting-up of agencies to deal with solid waste recycling. South Africa has legislation and stated programmes to deal with solid waste recycling. Nevertheless, there has been complexity with implementing recycling. This is the result of a lack of co-ordination between the role-players involved in the value chain of solid waste recycling. In order for the City of Johannesburg to increase the recycling of solid waste from 10% to optimal rates of between 50% and 60%, there should be co-ordination of programmes amongst all the role players. In addition, there should also be skills provision to existing and potential entrepreneurs operating the solid waste buy-back centres. All spheres of government should promote solid waste recycling business to potential entrepreneurs and the public in order to recycle 40% of the recyclable solid waste that is not being recycled. The establishment of a solid waste buy-back centre in the City of Johannesburg is economically viable with some buy-back centre realising a net-profit of least R5 000 and some more than R30 000 per month. In order for the solid waste buy-back centre to realise a net-profit of more than R30 000 per month, there must be more than 40 tons of recyclable solid waste received by the buy-back centre per month. This study has indicated that solid waste buy-back centres rely on the economies of scale. This means that the more recyclable solid waste is received and sold by the solid waste buy-back centres, the more profitable it becomes. / Thesis (M.B.A.)--North-West University, Vaal Triangle Campus, 2009.

Cannabinoids (CBs) are compounds that activate the CB1 and CB2 receptors. CB receptors mediate many different physiological functions, and cannabinoids have been reported to decrease tumor cell viability, proliferation, migration, as well as to modulate metastasis. In this thesis, the effects of cannabinoids on human colorectal carcinoma Caco-2 cells (Paper I) and mouse P19 embryonal carcinoma (EC) cells (Paper III) were studied.  In both cell lines, the compounds examined produced a concentration- and time-dependent decrease in cell viability. In Caco-2-cells, HU 210 and the pyrimidine antagonist 5-fluorouracil produced synergistic effects upon cell viability. The mechanisms behind the cytocidal effects of cannabinoids appear to be mediated by other than solely the CB receptor, and a common mechanism in Caco-2 and P19 EC cells was oxidative stress. However, in P19 EC cells the CB receptors contribute to the cytocidal effects possibly via ceramide production. In paper II, the association between CB1 receptor immunoreactivity (CB1IR) and different histopathological variables and disease-specific survival of colorectal cancer (CRC) was investigated. In microsatellite stable (MSS) cases there was a significant positive association of the tumor grade with the CB1IR intensity. A high CB1IR is indicative of a poorer prognosis in MSS with stage II CRC patients. Paper IV focused on the cytotoxic effects of cannabinoids during neuronal differentiation. HU 210 affected the cell viability, neurite formation and produced a decreased intracellular AChE activity. The effects of cannabinoids on embryonic development and survival were examined in Paper V, by repeated injection of cannabinoids in fertilized chicken eggs. After 10 days of incubation, HU 210 and cannabidiol (without CB receptor affinity), decreased the viability of chick embryos, in a manner that could be blocked by α-tocopherol (antioxidant) and attenuated by AM251 (CB1 receptor antagonist). In conclusion, based on these studies, the cannabinoid system may provide a new target for the development of drugs to treat cancer such as CRC. However, the CBs also produce seemingly unspecific cytotoxic effects, and may have negative effects on the neuronal differentiation process. This may be responsible for, at least some of, the embryotoxic effects found in ovo, but also for the cognitive and neurotoxic effects of cannabinoids in the developing and adult nervous system.

The increasing number of patients undergoing treatment with assisted reproductive techniques (ART) during the past years have led to the need of developing different methods for separation of spermatozoa that can be used for different fertilisation procedures and for freezing. Cryopreservation of spermatozoa includes preparation, freezing, storage and thawing. In this study two different cryomedia (Cryo Protec I and Cryo Protec II) for human spermatozoa were compared. The main outcome was spermsurvival rate for spermatozoa after freezing. Sperm viability was assessed using the Hypo-osmotic swelling test which is based on osmolality. A total of 86 samples of semen were used in this study (Cryo Protec I=38, Cryo Protec II=48). The survival rate between the two cryomedia did not differ much but Cryo Protectant I showed a small increase in survival for the spermatozoa after freezing. The Hypo-osmotic swelling test also showed similar values of viable spermatozoa for the two cryomedia both before and after freezing.

The main objective of this study was to investigate if natural honey can be used as a cryoprotecting agent (CP) in vitrification medium to improve the viability of vitrified-warmed bovine oocytes. The first study was conducted to investigate the dehydration capability of natural honey compared with sucrose, and to determine the proper concentration of honey-based medium and the optimum time for sufficiently safe dehydration of bovine oocytes. Matured cumulus-oocyte complexs (COCs) were denuded and introduced individually into different concentrations (0.25, 0.5, 1.0, 1.5 or 2.0 M) of honey and sucrose-based medium followed by rehydration in control media (TCM). Video images were recorded during dehydration and rehydration, and oocyte images were captured at 12 time intervals to calculate oocyte-volume changes during dehydration and rehydration. Results demonstrated that, in honey-based media, the maximum oocyte shrinkage was achieved after 60 sec exposure in 0.25M, 0.5M and 1.0M concentrations; while at higher concentrations 1.5M and 2.0M, the maximum dehydration occurred at 30 and 20 seconds respectively. In sucrose-based medium, the maximum oocyte shrinkage was achieved after 60 sec exposure in 0.25 or 0.5M concentrations. However, at higher concentrations (1M, 1.5M or 2M), the maximum dehydration occurred at 30, 20 and 10 sec. For rehydration, oocytes dehydrated in honey or sucrose-based medium were able to regain their original volume within 60-120 sec. However, oocytes dehydrated in higher concentrations (2M honey, and 1.5M and 2M sucrose) were rehydrated back to their original volume within 20 sec. This study concluded that natural honey and sucrose caused similar cell dehydration. Only oocytes dehydrated in 1M honey-based media reached maximal dehydration after 60 sec and equally regained original volume. Therefore, 1M of honey-based medium is suggested for sufficient and safe oocyte dehydration during vitrification. The second study was conducted to determine in vitro maturation (IVM), in vitro fertilization (IVF) and embryonic development of bovine oocytes vitrified in honey-based vitrifcation media. In Experiment 1, bovine COCs were randomly distributed in control group (non-vitrified; G1), 0.5M sucrose group (second control; G2), and 0.5M, 1M and 1.5M honey groups (G3, G4 and G5 respectively). The COCs were exposed to equilibration solution 1 (VS1) at ~ 22 oC for 5 min and to vitrification solution 2 (VS2) for 1 min, mounted on Cryotops and plunged into LN2. COCs were warmed in TCM and honey/sucrose medium at 38.5oC for 1 min, washed, matured in vitro (IVM), denuded, and immunostained to evaluate maturation. Maturation rate was significantly higher (80.7%) in control group (G1) than in vitrified groups (56, 52, 55 and 51% in G2, G3, G4 and G5, respectively) (P<.0001), whereas there was no significant difference among the vitrified groups (P>0.05). In Experiment 2, bovine COCs distributed in control (not vitrified, G1) and vitrified groups using 1M honey and 0.5M sucrose (G2 and G3 respectively), underwent for IVM, IVF and in vitro culture (IVC) for 9 days. Cleavage rate was significantly higher (P<.0001) in the control group (74%, G1, n=183) than rates of vitrified groups (51% in G2, n=137; and 42% in G3, n=131), whereas no differences among vitrified groups (P=0.0723). Rate of blastocyst formation was significantly higher (34%) in G1 than in the vitrified groups (P<.0001); however, blastocyst formation rates in the honey group were significantly higher (P=0.0026) than in the sucrose group (13% and 3% respectively). Addition of natural honey (1.0M; or 21.7%w/v) in vitrification medium can safely and sufficiently dehydrate bovine oocytes during vitrification procedure. The vitrification of bovine oocytes in 1M honey improved their post-warming maturation abtility and embryonic development.

Organizational adoption of information technology is an important decision for modern enterprises. Proper use of technology can increase benefits and competitive advantage. Inappropriate use could cause failure. Most previous literature focuses on treating technology adoption as a rational decision based on the functional needs of an organization. In many cases, however, technology adoption is not due to functional needs but environmental or peer pressure (called symbolic or ceremonial adoption). Different motivations for adoption may lead to different outcome. In addition, there is no generally accepted organizational adoption model that can include all key factors into consideration. Therefore, this study tries to understand what factors will affect an organization¡¦s adoption of a new information technology or use an information technology and whether different purposes (intangible value vs. tangible value) will affect the adoption or usage of information technology in organizations. The framework of this study was extended from the Fit-Viability Model to include the internal organizational factors and external environmental factors. We conducted a survey study and use PLS to analyze the collected data. The results show that the extended FV model can interpret organizational IT adoption and IT usage. The purpose of adoption did have effect on the intention to adopt information technology. In the symbolic adoption situation, TEF and viability had significant impacts on organizational intention to adopt; and in the functional adoption situation, TTF and viability had significant impacts on organizational intention to adopt. Different purposes of adoption have less significant effect on the actual use and performance of information system. TTF and actual use had significant impacts on organizational performance, and viability also had significant impacts on actual use of information technology in both situations. In other words, organizations can also benefit from IT adoption even if the original motivation was symbolic.

The increased popularity in mobile devices and technology has motivated business to adopt the technology for increased productivity. However, not much research has investigated the adoption of mobile technology. The purpose of this thesis is to study the factors that influence the adoption of mobile technology and to develop a model of mobile technology adoption. The model can serve as a foundation for future research and provide useful guidelines for organizations that plan to adopting mobile technology. The model suggests two categories of factors that determine the decision of adopting mobile technology: fit and viability. Fit measures whether the functional capabilities of mobile technology match the need of a task, whereas viability measures whether an organization is ready for the technology. This research develops instruments for measuring fit and viability. A survey was conducted to collect data for model evaluation. Major findings from the study include: (1) the fitness between task and technology had a direct positive impact on the success of mobile system adoption. (2) Personal characteristic and organizational viability had indirect positive impacts on the success of mobile system adoption via the mediation of system quality. (3) Personal characteristic and organizational viability had positive impacts on the information quality and system quality but had no impact to the service quality. (4) Only the system quality had a positive impact on the success of mobile system adoption.

Determination of an extender protocol which will enhance the viability of frozenthawed bovine spermatozoa will allow producers to obtain higher conception rates due to the increased survival rate of the spermatozoa. Ejaculates of six Brangus bulls (age=18 months) were evaluated for spermatozoal motility, acrosomal integrity, and morphological characteristics (collectively called spermatozoal viability) in two experiments to test our hypotheses that (1) the treatment combination of a 4 hr cooling duration and a 2 hr equilibration with glycerol will result in optimum spermatozoal characteristics after freezing and thawing and (2) rank of three selected extenders relative to their effects on spermatozoal viability after freezing and thawing will be egg yolk-citrate (EC), egg yolk-tris (IMV), and skim milk (milk). In experiment 1, an ejaculate from each bull was partially extended and cooled to 4 ºC for either 2 or 4 hr and then allowed to equilibrate with the glycerolated extender for 2, 4, or 6 hr. Spermatozoal viability was assessed at 0, 3, 6, and 9 hr after thawing. In experiment 1, 4 hr of cooling resulted in a higher percentage of motile spermatozoa than did 2 hr of cooling. The 2 hr equilibration with glycerol yielded lower percentages of motile spermatozoa, acrosomal integrity, and morphologically normal spermatozoa than 4 and 6 hr equilibration durations with glycerol. In experiment 2, we observed a decrease in spermatozoal viability for all three extenders upon freezing and thawing. Viability of frozen-thawed spermatozoa extended in the milk was reduced for all incubation durations, and the IMV extender had a higher percentage of motile spermatozoa than the EC extender at 6 hr of incubation. A higher percentage of intact acrosomes was observed with the IMV extender; however, the EC extender had a higher percentage of morphologically normal spermatozoa than the IMV extender. Our results indicate that at cooling duration of 4 hr and a 4 hr equilibration with glycerol provide the highest level of spermatozoal viability post-thaw of the treatments evaluated and that the IMV extender enhances the percentage of spermatozoa with an intact acrosome for frozenthawed spermatozoa over the EC and skim milk extenders.

Fruits and vegetables are known to play an important role in human health due to the range of phytochemicals they contain. Twenty-one peach genotypes and 45 plum genotypes with different flesh and skin color were analyzed for their antioxidant content and antioxidant activity. Anthocyanin content, phenolic content and antioxidant activity were higher in red-flesh than in light-colored flesh peaches. Carotenoid content was higher in yellow-flesh peaches. Among the peaches, the antioxidant activity was well correlated with phenolic content. The anthocyanin content among the plums increased with the red color intensity. Red-flesh plums generally had higher phenolic content than the other plums. Antioxidant activity was higher in red-flesh genotypes; however, it was strongly correlated only with the phenolic content in light-colored flesh plums. Extracts from selected genotypes of peaches and plums and their fractions were evaluated against two breast cancer cell lines (MDA-MB-435 and MCF-7) and one non-cancerous breast line (MCF-10A). The cells were cultured in the presence of peach and plum extracts and their fractions at various concentrations (0-500 µg/ml) and the cell viability and antiproliferation activity was evaluated by MTT assay and Coulter Counter. There was a dose-dependent reduction on cell viability of estrogen-negative MDA-MB-435 breast cancer cells. Only weak activity against MCF-7 was observed at high extract concentrations. There was no activity against MCF-10A after 24 h treatment. Fraction I, which consists of mainly phenolic acids such as chlorogenic acid and a caffeic acid derivative, reduces MDA-MB-435 breast cancer cell viability with the lowest IC50. The second most effective fraction was Fraction II which contained anthocyanins. Fraction III (flavonols) and Fraction IV (polymerized compounds) had no effect on the cell lines. Phenolic acids present in fraction I induced apoptosis in MDA-MB-435 estrogen receptor-negative cell line. Fraction I did not induced apoptosis in MCF-10A, a noncancerous cell line even at higher concentrations than the ones tested in MDA-MB- 435. Apoptosis induced by Fraction I was caspase 3 and PARP independent. After treatment with 50 µg of chlorogenic acid equivalent/ml there was an activation of p- ERK.