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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
61

A importância dos mecanismos comportamentais de resistência para a dinâmica populacional de abelhas Apis mellifera e o parasita Varroa destructor

Santos, Joyce de Fgueiró 06 June 2014 (has links)
Submitted by Joyce de Figueiró Santos (joycefigueiro@gmail.com) on 2014-07-21T14:48:02Z No. of bitstreams: 1 mestrado(1).pdf: 12859151 bytes, checksum: cce9beb4e56c9706618a1d6361e58fd9 (MD5) / Approved for entry into archive by Janete de Oliveira Feitosa (janete.feitosa@fgv.br) on 2014-07-22T21:09:53Z (GMT) No. of bitstreams: 1 mestrado(1).pdf: 12859151 bytes, checksum: cce9beb4e56c9706618a1d6361e58fd9 (MD5) / Approved for entry into archive by Marcia Bacha (marcia.bacha@fgv.br) on 2014-07-24T17:35:11Z (GMT) No. of bitstreams: 1 mestrado(1).pdf: 12859151 bytes, checksum: cce9beb4e56c9706618a1d6361e58fd9 (MD5) / Made available in DSpace on 2014-07-24T17:35:59Z (GMT). No. of bitstreams: 1 mestrado(1).pdf: 12859151 bytes, checksum: cce9beb4e56c9706618a1d6361e58fd9 (MD5) Previous issue date: 2014-06-06 / The ectoparasitic mite Varroa destructor that parasitize honey bees has become a global problem. Although this mite is unlikely to, by itself, cause the mortality of hives, it plays an important role as a vector for many viral diseases. These diseases are identified as some of the most important reasons for the Colony Collapse Disorder. The effects of V.destructor infestation are disparate in different parts of the world. Greater morbidity - in the form of colony losses - has been reported in colonies of European honey bees (EHB) in countries of Europe, Asia and North America. However, this mite has been in Brasil for many years and there are no reports of losses of Africanized honey bees (AHB) colonies. Studies carried out in Mexico showed that some resistance behaviors to the Varroa mite - especially grooming and hygienic behavior - appear to be different in each subspecies. Could those mechanisms explain why the AHB are less susceptible to Colony Collapse Disorder? In order to answer this question, we propose a mathematical model based on differential equations, to analyze the role of these resistance mechanisms in the overall health of the colony, and it’s ability to face environmental challenges. / Os ácaros ectoparasitas Varroa destructor, que parasitam as abelhas tornaram-se um problema global. Embora seja pouco provável que estes ácaros, por si só, provoquem a mortalidade das colmeias, eles desempenham um importante papel como vetor de muitas doenças virais. E estas doenças são identificados como algumas das mais importantes razões para a Desordem do Colapso das Colônias. Os efeitos da infestação do V.destructor são distintas em diferentes partes do mundo. Maiores mortalidades de colônias têm sido relatadas em colônias de abelhas européias (AE) em países da Europa, Ásia e América do Norte. No entanto, este ácaro está presente no Brasil já por muitos anos e não existem relatos de perdas em colônias das abelhas africanizadas (AA). Estudos realizados no México mostraram que alguns comportamentos de resistência ao ácaro Varroa - especialmente o grooming e o comportamento higiênico - são diferentes em cada uma das subespécie. Poderiam então esses mecanismos explicar por que as abelhas africanizadas são menos suscetíveis à Desordem do Colapso das Colônias? A fim de responder a esta pergunta, propomos um modelo matemático baseado em equações diferenciais, com o objetivo de analisar o papel desses mecanismos de resistência na saúde geral da colônia e na capacidade da colônia para enfrentar desafios ambientais.
62

Grip, slip, petals, and pollinators : linking the biomechanics, behaviour and ecology of interactions between bees and plants

Pattrick, Jonathan Gilson January 2018 (has links)
The ability to grip on petal surfaces is of crucial importance for the interactions between bees and flowers. In this thesis, I explore the biomechanics of attachment and morphological diversity of bee attachment devices, linking this to the behavioural ecology of bee interactions with flowers. Attachment devices come in two main kinds: claws or spines, and adhesive pads. Claw functioning is poorly described, particularly in terms of how their performance depends on body size, claw geometry, and surface roughness. Claw attachment performance was investigated using several insect species, each covering a large range of body masses. Weight-specific attachment forces decreased with body size, with claw sharpness seemingly playing a role. In bees there is considerable interspecific variation in tarsal claw morphology. This variation, and arolia presence/absence, was categorised for the large bee family Apidae. Cleft/bifid claws were shown to be present in the majority of the Apidae, often with differences between sexes and clades. Using Bombus terrestris, there was no evidence that cleft claws are important for pollen collection; however, I found that the inner tooth of cleft claws can act as a backup if the main tooth breaks. Although this may be one function of cleft claws, there are clearly other unresolved functions well worth further exploration. Investigations were undertaken to explore how petal surface roughness affects bee foraging behaviour. Lab-based foraging trials on B. terrestris visiting artificial flowers varying in slope, surface texture and sugar reward revealed a trade-off between the biomechanical difficulty of visiting and handling the ‘flowers’ and the quality of the reward offered. Flowers that were difficult to grip were often avoided even if they offered a higher reward. To further investigate reward preferences of bees, the effect of sucrose concentration on honey stomach offloading times was also explored. Although the majority of petals do have a rough surface, some have slippery petals. In the field, bumblebees avoided landing on slippery hollyhock petals in favour of the easy-to-grip staminal column. In contrast, honey bees, which are smaller and have larger adhesive pads, landed on both the staminal column and the petals. Slippery petals may be an adaptation to increase contact with plant reproductive structures. Grip is also important to allow the honey bee parasite Varroa destructor to climb on to their host. Attachment forces experiments found that V. destructor could support > 300 times their body mass on honey bees, giving them strong attachment even when bees attempt to remove them through grooming. A grooming-based device for treating V. destructor was tested in an apiary trial. The device was ineffective, providing valuable information for beekeepers considering using this product. In summary, this thesis improves our understanding of the biomechanics of attachment as well as identifying several important aspects of grip in bee-plant interactions.
63

Application d’une stratégie de lutte intégrée contre le parasite Varroa destructor dans les colonies d’abeilles mellifères du Québec

Giovenazzo, Pierre 04 1900 (has links)
Le parasite Varroa destructor provoque depuis plus de 30 ans la perte de nombreuses colonies à travers le monde. L’utilisation d’acaricides de synthèse s’est avérée inefficace au Canada et ailleurs dans le monde à la suite de la sélection de varroas résistants. Dans ce contexte, il est devenu impératif de trouver de nouveaux moyens pour contrôler cette peste apicole. Ce travail original de recherche a pour but de déterminer les paramètres fondamentaux d’une lutte intégrée contre la varroase fondée sur l’utilisation périodique de différents pesticides organiques (l’acide oxalique, l’acide formique et le thymol) associée à des seuils d’interventions. Les seuils d’intervention ont été déterminés à l’aide de régressions linéaires entre les taux de parasitisme par V. destructor et la formance zootechnique des colonies d’abeilles mellifères (production de miel et force des colonies). Un total de 154 colonies d’abeilles du Centre de recherche en sciences animales de Deschambault (CRSAD) ont été suivies de septembre 2005 à septembre 2006. Les seuils calculés et proposés à la suite de cette recherche sont de 2 varroas par jour (chute naturelle) au début mai, 10 varroas par jour à la fin juillet et de 9 varroas par jour au début septembre. L’efficacité des traitements organiques avec l’acide oxalique (AO), l’acide formique (AF) et le thymol a été vérifiée en mai (avant la première miellée) en juillet (entre deux miellées), en septembre (après la miellée et pendant le nourrissage des colonies) et en novembre (avant l’hivernage). L’acide oxalique a été appliqué en utilisant la méthode d’égouttement (4% d’AO p/v dans un sirop de sucrose 1 :1 p/v). L’acide formique a été appliquée sous forme de MiteAwayII™ (tampon commercial imbibé d’AF 65% v/v placé sur le dessus des cadres à couvain), Mitewipe (tampons Dri-Loc™ 10/15cm imbibés de 35 mL d’AF 65% v/v placés sur le dessus des cadres à couvain) ou Flash (AF 65% coulé directement sur le plateau inférieur d’une colonie, 2 mL par cadre avec abeilles). Le thymol a été appliqué sous forme d’Apiguard™ (gélose contenant 25% de thymol p/v placée sur le dessus des cadres à couvain). Les essais d’efficacité ont été réalisés de 2006 à 2008 sur un total de 170 colonies (98 appartenant au CRSAD et 72 appartenant au privé). Les résultats montrent que les traitements de printemps testés ont une faible efficacité pour le contrôle des varroas qui sont en pleine croissance durant cette période. Un traitement avec l’AF à la mi-été permet de réduire les taux de parasites sous le seuil en septembre mais il y a risque de contaminer la récolte de miel avec des résidus d’AF. Les traitements en septembre avec le MiteAwayII™ suivis par un traitement à l’acide oxalique en novembre (5 mL par égouttement entre chaque cadre avec abeilles, 4% d’AO p/v dans un sirop de sucrose 1 :1 p/v) sont les plus efficaces : ils réduisent les niveaux de varroase sous le seuil de 2 varroas par jour au printemps. Nos résultats montrent également que les traitements réalisés tôt en septembre sont plus efficaces et produisent des colonies plus fortes au printemps comparativement à un traitement réalisé un mois plus tard en octobre. En conclusion, ce travail de recherche démontre qu’il est possible de contenir le développement de la varroase dans les ruchers au Québec en utilisant une méthode de lutte intégrée basée sur une combinaison d’applications d’acaricides organiques associée à des seuils d’intervention. / For nearly 30 years, Varroa destructor has been responsible for the loss of many honey bee colonies around the world. The continued use of synthetic acaricides has resulted in their reduced efficacy against this pest in Canada and in other countries because of the selection of resistant mite populations. With this situation still present, it has become of utmost importance to develop efficient methods to control this apicultural pest. The major goal of this original work is to determine the fundamental parameters underlying the use of an integrated pest management (IPM) strategy against the varroa mite. The IPM strategy developed in this research is based on the periodic use of organic pesticides (oxalic acid, formic acid and thymol) and treatment threshold. Treatment thresholds were determined from linear regressions between the varroa mite levels and the zootechnical performances (honey production and colony strength) of honey bee colonies. A total of 154 honey bee colonies from the livestock of the “Centre de recherche en sciences animales de Deschambault” (CRSAD) were monitored from September 2005 to September 2006. Based on our findings, we propose economic treatment thresholds for three periods in the year: early May, late July and early September that are respectively 2, 10 and 9 varroa mites per day. Efficacy of the various organic treatments: formic acid (FA), oxalic acid (OA) and thymol was evaluated in May (before the first honey flow), in July between two honey flows, in September (after the honey flow and before the fall feeding of colonies) and in November (before wintering). OA was applied using the trickling method (4% OA w/v in a sucrose syrup 1:1 w/v). FA was applied using MiteAwayII™ (pads imbedded with FA 65% v/v placed on top of brood frames), Mitewipe (Dri-Loc™ pads 10/15cm imbedded with 35 mL FA 65% v/v placed on top of brood frames), Flash (FA 65% poured directly on the bottom board of colonies, 2 mL per frame with bees). Thymol was applied using Apiguard™ (gel with 25% de thymol w/v placed on top of the brood frames). Efficacy trials were realised from 2006 to 2008 on a total of 170 colonies (98 from the CRSAD and 72 owned by a commercial beekeeper). Results show that treatments applied in spring give low efficacy on reducing varroa mite populations that are in full growth at this time because of large amounts of brood available for mite reproduction. Application of a FA treatment in mid-summer offers the opportunity to reduce mite populations at the 11 mites per day September threshold but FA summer application is accompanied by a risk of incorporating residues in the harvested honey. Application of MiteAwayII™ in September followed by an oxalic acid treatment in November (trickling method 4% OA w/v in a sucrose syrup 1 :1 w/v, 5 mL between frames with bees) gave the best efficacy results: varroa mite levels are reduced below the 2 mites per day spring threshold. Our results also show that an early September management strategy of colonies for winter preparation (varroa treatment and fall feeding) gives greater varroa control, higher colony winter survival and stronger colonies in spring when compared to a later treatment in October. In conclusion, this work shows that varroa mite control in honey bee colonies in Québec is possible by using an integrated pest management strategy based on the application of a combination of organic acaricides in association with treatment thresholds.
64

Varroa destructor chez l’abeille domestique (Apis mellifera) : impacts sur l’hémolymphe et les infections secondaires

Cournoyer, Antoine 11 1900 (has links)
L’abeille domestique (Apis mellifera) est un insecte qui contribue à l’agriculture par sa pollinisation. Le taux élevé des mortalités hivernales des colonies est préoccupant depuis des décennies au Canada. Plusieurs facteurs sont impliqués, particulièrement Varroa destructor; un parasite qui se nourrit du corps gras de l’abeille. Le développement d’outils adaptés permettrait un meilleur suivi des colonies. Le projet consiste à corréler l’infestation de varroa avec les concentrations en sucres sériques et les co-infections (virales et bactériennes). Cette étude compare dans le temps six ruches fortement infestées et six ruches traitées (témoins). Un prélèvement d’hémolymphe a été effectué pour mesurer les concentrations en sucres en utilisant un glucomètre humain préalablement validé. Les concentrations en sucres (glucose et tréhalose) dans l’hémolymphe étaient significativement plus faibles (p<0.001) dans les ruches fortement infestées que les témoins en septembre. L’analyse RT-PCR multiplexe de six virus (DWV A/B, BCQV, KBV, IAPV et ABPV) a démontré que les ruches fortement infestées présentent une infection simultanée virale avec des charges plus élevées que chez les ruches témoins (p<0.05) pour la majorité des virus, sauf pour ABPV. Chez les ruches fortement parasitées, les charges virales pour DWVA et BQCV sont plus élevées en septembre qu’en juillet (p≤0.0001). Serratia marcescens a été seulement détectée dans une ruche infestée et une ruche témoin. Une exposition continue et élevée à varroa occasionne, en automne, une augmentation des charges virales et une diminution des sucres, suggérant une altération de l’immunité, du métabolisme et des réserves. Ces paramètres provoquent une faiblesse et une mortalité des colonies. / The European honeybee (Apis mellifera) contributes to the agriculture by its pollination; however, the mean overwintering loss rate of colonies over the last decades in Canada is worrisome. Varroa destructor, which feeds on the fat bodies of honeybees, is considered one of the most important causes of bee colony declines. The development of adapted diagnostic tools would improve the monitoring of honeybee health. This project aims to correlate the infestation by varroa to the hemolymph sugar concentrations (trehalose and glucose) and bacterial and viral coinfections. Six highly infested and six treated hives were compared over time. Pooled hemolymph of honeybees was collected for sugar concentration measurements using a previously validated portable glucometer. The hemolymph samples were also submitted for bacteriology. Multiplex RT-PCR analyses were performed on pooled honeybees for six viruses: Deformed wing virus A and B (DWV-A/B), Bee Queen Cell Virus (BQCV), Acute Bee Paralysis Virus (ABPV), Kashmere Bee Virus (KBV), Israeli Acute Paralysis Virus (IAPV). The results show that, in September, sugar concentrations in hemolymph were significantly lower in highly infested hives (p<0.001). Infested hives showed markedly higher viral loads (p<0.05), except for ABPV. Viral loads were significantly higher (p≤0.0001) in September than in July for DWV-A and BQCV. Serratia marcescens was only detected in one infested hive and one control. Overall, a continued and severe exposure to varroa leads to increased viral charges and decreased sugar concentrations, suggesting alterations in immunity, metabolism and reserve mobilization. All these parameters contribute to the weakening and mortality of the colonies.

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