Made available in DSpace on 2017-07-10T17:37:31Z (GMT). No. of bitstreams: 1 Tania Pires da Silva.pdf: 493041 bytes, checksum: 3d9daa71e02d7c679bf5b90cf21bb3c5 (MD5) Previous issue date: 2008-12-09 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / The aim of the work was to evaluate the phytotechnical performance of different rootstocks in the formation level on field conditions and the initial development of the graft, as well as the application of auxiliaries technicians to the root potential increment of the rootstocks cuttings of VR 043-43 in nursery conditions. Ligneous cuttings from grapevine VR 043-43 , measuring 20 cm, were stratified for 48h (under the temperature of 4 oC), part of the cuttings were kept under environment temperature (25 } 5 oC). All the cuttings were treated with indolbutyric acid (IBA) in different concentrations of 0, 1.000, 2.000 and 3.000 mgL-1, associated and not associated to boric acid at concentration of 150 μg L-1 for 10sec. The cuttings were planted into polyethylene plastic bags (capacity of 2,5L), filled with clay soil. They were kept in nursery made of shading screen providing 50% of luminosity. The evaluations occurred 120 days after the experiment deployment. In November, the seedlings originated from the rooted cuttings of 17 grapevine rootstocks ( SO4 , Teleki 8B , Harmony , Golia , Riparia de Traviu , Paulsen , 420 A , 99R , 5C , RR101-14 , Kober 5BB , Rupestris Du Lot , IAC 313 Tropical , IAC 766 Campinas , IAC 572 Jales , VR 043-43 and IAC 571-6 Jundiai ) were taken to the field under subtropical conditions (24o33 40 S and 54o04 12 W, 420 m height and Cfa Koppen climate, with annual maximum average temperature of 28,5 oC and minimum of 17,5 oC). After four months, it was evaluated the anthracnose and rust severity on the grapevine leaf sprouting from the rootstocks and more five months of vigor (total branch number, average diameter, biggest branch length and total leaf area fresh mass). In the sequence, it was performed winter graft using BRS Violeta . The evaluated characteristics for this part of the experiment were percentage of tissue union, diameter and graft average length, which happened after 60 days of grafting. It was concluded that the stratification of the cutting is not fundamental to increase the efficiency of the rooting process of the rootstock VR 043-43 . The cuttings should only be treated with IBA at 3.000 mg L-1 in association to boric acid. The rootstocks IAC 572 Jales and IAC 313 Tropical were resistant to the anthracnose and rust. The rootstock IAC 572 Jales was the one that showed greater performance on field, before grafting. The rootstocks SO4 , Harmony , Paulsen and IAC 766 Campinas showed greater vigor to the development of the graft BRS Violeta / O trabalho teve como objetivo avaliar o desempenho vegetativo de diferentes porta-enxertos na fase de formacao a campo e o desenvolvimento inicial do enxerto, bem como o emprego de tecnicas auxiliares para o incremento do potencial rizogenico de estacas do porta-enxerto VR 043-43 em condicoes de viveiro. Estacas caulinares lenhosas de 20 cm comprimento do porta-enxerto de videira VR 043-43 , estratificadas por 48 h (sob temperatura de 4 oC) ou não (ambiente de 25o } 5 oC), foram tratadas em solucao de acido indolbutirico (AIB) nas concentracoes de 0, 1.000, 2.000 e 3.000 mg L-1, associado ou nao com acido borico a 150 μg L-1, por 10 seg. As estacas foram plantadas em saco plastico de polietileno (capacidade de 2,5 L), contendo solo com textura argilosa como substrato e mantidas sob viveiro constituido de tela de sombreamento de 50% de luminosidade, sendo avaliadas apos 120 dias. Em novembro, mudas oriundas de estacas enraizadas de 17 porta-enxertos de videira ( SO4 , Teleki 8B , Harmony , Golia , Riparia de Traviu , Paulsen , 420 A , 99R , 5C , RR101-14 , Kober 5BB , Rupestris Du Lot , IAC 313 Tropical , IAC 766 Campinas , IAC 572 Jales , VR 043-43 e IAC 571-6 Jundiai ) foram levadas a campo, em condicoes subtropicais (24o33 40 S e 54o04 12 W, altitude de 420 m e clima Cfa Koppen, com temperatura media maxima anual de 28,5 oC e minima de 17,5 oC). Passados quatro meses, avaliou-se a severidade da antracnose e ferrugem da videira nas folhas das brotacoes dos porta-enxertos e mais cinco meses o vigor dos mesmos (numero total de ramos, diametro medio, comprimento do maior ramo e massa fresca total media dos ramos). Em seguida, realizou-se a enxertia invernal com garfos da videira BRS Violeta e avaliou-se apos 60 dias a porcentagem de pegamento, o diametro e o comprimento medio do enxerto. Concluiu-se que a estratificacao das estacas nao e fundamental para que haja maior eficiencia do processo de enraizamento do porta-enxerto VR 043-43 , devendo as estacas apenas serem tratadas com 3.000 mg L-1 de AIB, associada ao acido borico. Os porta-enxertos IAC 572 Jales e IAC 313 Tropical foram resistentes a antracnose e a ferrugem. O porta-enxerto IAC 572 Jales foi o que promoveu maior desempenho a campo, previamente a operacao de enxertia. Os porta-enxertos SO4 , Harmony , Paulsen e IAC 766 Campinas promoveram maior vigor ao desenvolvimento do enxerto de BRS Violeta

Made available in DSpace on 2017-07-10T17:40:48Z (GMT). No. of bitstreams: 1 2013_Tese_Tatiani_Alano_Modolon.pdf: 1381280 bytes, checksum: 030e95c78878a1de4045f816336a2a83 (MD5) Previous issue date: 2013-12-16 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / The current system of corn production, provide high productivity while favoring pest outbreaks, such as the stink bug Dicheplos melacanthus and fall armyworm Spodoptera frugiperda. Conventional control strategies have been ineffective so far. This study aimed to evaluate the impact of homeopathic preparations on the development of S. frugiperda and D. melacanthus in corn plants. To study the consumption of fall armyworm S. frugiperda seeds were treated before planting by soaking in distilled water and homeopathic preparations at a dose of 1 mL.L-1. After soaking, the seeds were sown in pots. In experiments with fall armyworm in addition to the seed treatment (a), the dynamizations 12, 36, 60 and 84CH (centesimal hahnemaniana) of Silicea were applied in spraying (b) or irrigation (c), with an first application was made just after emergence, the second in V2 stage, and the third application in V3 stage of corn plants. Leaf sections of plants at V6 stage derived from the respective treatment were provided daily to caterpillars in individualized test tubes and maintained in the development room at 25 ± 3 °C and 14 hours photoperiod. Were evaluated consumption of leaves, formation and sex ratio of pupae and adults, fertility, fecundity, longevity of adult and food preference in free choice test. For the experiment with green-belly stink bug, D. melacanthus, homeopathic preparations Nux vomica was also applied as seed treatment (a) the dynamizations 12, 36, 60 and 84DH (decimal order of Hering) and combined with spraying (b) or irrigation (c), and started ten days after sowing and followed within three days until the 22th day after sowing, totaling five applications. Five days after the last application, fresh and dry mass of shoots and roots, root volume, primary root length, height and stem diameter of corn plants were evaluated. On the sixth day after the last treatment, the plots replicas were subjected to stress by the insect. Corn plants were infested with a D. melacanthus bug for 36 hours. Ten days after removal of the bug, the classification of attacked plants was performed through a rating scale of damage. Data analysis showed that corn plants sprayed or irrigated with 36CH Silicea interfered in the consumption and utilization of food. In addition, it provided greater amount of pupae and adults of deformed S. frugiperda. Plants sprayed or irrigated on soil with 36CH Silicea also interfered with fertility and fecundity of S. frugiperda, providing shorter oviposition and post-reproductive periods, as well as fewer egg masses, eggs per ovoposition and eggs per female. The results suggest that larvae of S. frugiperda have difficulty while feeding on corn plants sprayed or irrigated with 36CH Silicea. Corn plants treated with 36CH Silicea homeopathic preparation, applied by spraying the shoots or by irrigating the soil, interfered with the feeding and, therefore, with fertility and fecundity of S. frugiperda, reducing insect population. The method of application of homeopathic preparations Nux vomica more suitable for the corn crop was in the through irrigation on soil. Nux vomica at 33, 36 and 38DH irrigated to soil favored the early development of shoots and roots of corn plants. Corn plants irrigated Nux vomica 36 and 38DH were not damaged by green-belly stink bug D. melacanthus, showing the effect of repellency or not food preference / O sistema atual de produção de milho, proporcionam elevada produtividade ao mesmo tempo que favorece a surtos epidêmicos pragas, como o percevejo Dichelops melacanthus e a lagarta Spodoptera frugiperda. Estratégias de controle convencionais têm sido ineficazes até o momento. O presente estudo teve o objetivo avaliar o impacto de preparados homeopáticos no desenvolvimento de S. frugiperda e D. melacanthus em plantas de milho. Para estudo do consumo da lagarta S. frugiperda sementes foram tratadas antes do plantio por imersão em água destilada e nos preparados homeopáticos na dose de 1 mL.L-1. Após a embebição, as sementes foram semeadas nos vasos. Nos experimentos com a lagarta-do-cartucho, além do tratamento de semente (a), as dinamizações 12, 36, 60 e 84CH (ordem centesimal hahnemaniana) de Silicea foram aplicadas em pulverização (b) ou irrigação (c), sendo a primeira aplicação logo após a emergência, a segunda no estádio V2 e a terceira no estádio V3 das plantas. Secções foliares das plantas em estágio V6 oriundas do respectivo tratamento foram diariamente fornecidas às lagartas individualizadas em tubos de ensaio e mantidas em sala de desenvolvimento em 25 ± 3 °C e fotoperíodo 14 horas. Foram avaliados o consumo das folhas, formação e razão sexual de pupas e adultos, fertilidade, fecundidade, longevidade de adultos e preferência alimentar em ensaio de livre escolha. Para experimentos com o percevejo barriga-verde, D. melacanthus, preparado homeopático Nux vomica também foi aplicado via tratamento de semente (a) nas dinamizações 12, 36, 60 e 84DH (ordem decimal de Hering) e combinado com pulverização (b) ou irrigação (c), sendo iniciados dez dias após a semeadura e seguidas no intervalo de três dias até o 22° dia após a semeadura, totalizando cinco aplicações. Cinco dias após a última aplicação dos tratamentos foram avaliados massa fresca e seca de parte aérea e de raízes, volume das raízes, comprimento da raiz primária, altura e diâmetro do colmo das plantas de milho. No sexto dia após a última aplicação dos tratamentos, as parcelas réplicas foram submetidas ao estresse pelo inseto. As plantas de milho foram infestadas com um percevejo D. melacanthus por 36 horas. Dez dias após a retirada do percevejo foi realizada a classificação plantas atacadas através de escala de notas de dano. A análise dos dados mostrou que plantas de milho pulverizadas ou irrigadas com Silicea 36CH interferiram no consumo e utilização do alimento, além de proporcionarem maior quantidade de pupas e adultos de S. frugiperda deformados. Plantas pulverizadas ou irrigadas no solo com Silicea 36CH também interferiram na fertilidade e fecundidade de S. frugiperda proporcionando menor período de oviposição e pós-reprodutivo, menor número de massas de ovos, ovos por postura e ovos por fêmea. Os resultados sugerem que lagartas de S. frugiperda têm dificuldade ao se alimentarem de plantas de milho pulverizadas ou irrigadas com Silicea 36CH. Plantas de milho tratadas com o preparado homeopático Silicea em 36CH aplicado através de pulverização na parte aérea ou por irrigação no solo interferiram na alimentação e, conseqüentemente, na fertilidade e fecundidade de S. frugiperda, reduzindo assim, a população do inseto. O método de aplicação do preparado homeopático Nux vomica mais adequado para a cultura do milho foi através da irrigação no solo. Nux vomica na 33, 36 e 38DH irrigado ao solo favoreceu o desenvolvimento inicial de parte aérea e raízes de plantas de milho. Plantas de milho irrigadas com Nux vomica 36 e 38DH não foram danificadas pelo percevejo barriga-verde D. melacanthus, evidenciando o efeito de repelência ou não preferência alimentar

Comment concilier productivité et réduction de l'usage des pesticides ? Le développement de l'oïdium, maladie majeure en viticulture, peut être limité (service de régulation de l'oïdium) par un faible niveau de développement végétatif de la vigne associé à une forte porosité du couvert. Or, de telles caractéristiques limitent également la quantité de rayonnement intercepté par la vigne, et donc la production de biomasse par photosynthèse, et finalement le rendement (service d'approvisionnement). Peut-on augmenter la régulation de l'oïdium sans diminuer le rendement de la vigne ? Autrement dit, existe-t-il une relation de compromis entre ces deux services ? Pour répondre à cette question, deux expérimentations ont été mobilisées : une expérimentation au champ réalisée près de Montpellier de 2010 à 2012 (Syrah), et un réseau de témoins non traités suivis par l'IFV dans la région de Bordeaux de 2007 à 2013. L'analyse des données issues de ces expérimentations a permis de montrer (i) que l'effet des stress hydrique et azoté sur la formation des inflorescences dans les bourgeons latents à la floraison de l'année n-1 détermine 65 à 70 \% du rendement de la vigne lors de l'année n ; (ii) que l'effet du développement végétatif de la vigne sur le développement de l'oïdium n'est significatif que dans le cas d'épidémies de précocité moyenne, dont les premiers symptômes ont été détectés sur feuilles entre la floraison et la fermeture de la grappe. Le développement végétatif de la vigne à la floraison est donc un indicateur pertinent du service de régulation de l'oïdium. Ces résultats ont ensuite permis de confirmer expérimentalement l'hypothèse de l'existence d'un compromis entre rendement de la vigne et régulation de l'oïdium et d'identifier le stress hydrique à la floraison comme un déterminant partagé de ces deux services. Ce dernier point a alors été transcrit dans un modèle simple dans lequel le rendement de la vigne de l'année n dépend principalement de l'année n-1, et le développement végétatif dépend seulement de l'année n. L'analyse des propriétés mathématiques de ce modèle a permis de montrer que la réalisation de compromis favorables entre ces deux services (i.e. un fort niveau des deux services) est possible mais déterminée par l'occurrence de séquences climatiques particulières (une année humide suivie d'une année sèche). Ainsi, les compromis favorables ne peuvent être atteints deux années consécutives et ne sont donc pas stables dans le temps. Les règles de décisions d'application des traitements phytosanitaires pourraient ainsi être adaptées en fonction de la variabilité climatique inter-annuelle et de ses conséquences sur les compromis entre le rendement de la vigne et la régulation des maladies cryptogamiques. / How to combine crop productivity and reduction in pesticide use ? A reduction in plant growth and an increase in crop canopy porosity has been shown to limit the development of the grapevine powdery mildew (powdery mildew regulation service), which is a major disease in viticulture. However, this could also limit grapevine yield through a reduction in light interception and biomass production through photosynthesis. Can regulation of powdery mildew be increased without grapevine yield impairment ? In other words, do these two services trade off ? To address this question, two experiments were used. A field experiment was conducted in Montpellier from 2010 to 2012 on Shiraz, and a network of non-sprayed vineyard plots was monitored by the French Institute of Vine and wine in the Bordeaux region from 2007 to 2013. Data analyses showed that: (i) 65 to 70 \% of grapevine yield in year n were determined by the effect of water and nitrogen stresses on inflorescence formation in the latent buds at flowering in year n-1; (ii) the effect of grapevine vegetative development on powdery mildew depended on epidemic earliness and was significant only when first symptoms were detected on leaves between flowering and bunch closure. Grapevine vegetative development at flowering was therefore a relevant indicator of the powdery mildew regulation service. Based on these results, the hypothesis of a trade-off between grapevine yield and powdery mildew regulation was experimentally confirmed and water stress at flowering was identified as a shared driver of these two services. Then, a simple model was built that accounted for the effect of water stress at flowering on the two services, where grapevine yield depended mostly on year n-1 and grapevine vegetative development depended only on year n conditions. Analysis of the model's mathematical properties showed that "win-win" scenarios (high level of both services) did exist and were reached when specific climatic sequences occured (a wet year followed by a dry year). The "win-win" scenarios could not be reached two consecutive years and were therefore temporally unstable. Decision rules for pesticides application may be adapted in function of the inter-annual climatic variability and its effect on the trade-off between grapevine yield and regulation powdery mildew.

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