Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / O objetivo deste trabalho foi avaliar a compatibilidade do tratamento de sementes do feijão-caupi com fungicidas e as estirpes recomendadas para esta cultura. Em laboratório avaliou-se o efeito na sobrevivência de rizóbios inoculado nas sementes de feijão-caupi e tratadas ou não com fungicidas à base fludioxonil, carbendazim, carbendazim + thiram e carboxin + thiram, nas doses recomendas para cultura da soja, e inoculadas com as estirpes recomendadas para cultura. Em casa de vegetação foi avaliado a nodulação e a massa seca da parte aérea de feijão-caupi a partir de sementes inoculadas e tratadas com os fungicidas, além de avaliar o efeito dos fungicidas na germinação e desenvolvimento de plântulas em cinco cultivares de feijão-caupi. No campo foi avaliado a nodulação e a produtividade de grãos de feijão-caupi inoculadas com a estirpe BR3262 e tratadas ou não com fungicidas. Não foi observado efeito dos fungicidas sobre a sobrevivência dos rizóbios nas sementes, tampouco redução da nodulação das plantas além de não influir na germinação e no desenvolvimento das plantas de feijão-caupi, em condições de casa de vegetação. Em condições de campo não foi observado efeito dos fungicidas na nodulação tampouco no rendimento de grãos. Com isto, a utilização de fungicidas no tratamento de sementes de feijão-caupi e compatível com a inoculação. / The aim of this study was to evaluate the compatibility of the seed treatment with fungicides cowpea and strains recommended for this crop. In laboratory evaluated the effect on survival of rhizobia inoculated seeds of cowpea and treated or untreated with fungicides fludioxonil, carbendazim, carbendazim + thiram and carboxin + thiram, in doses recommended for soybean, and inoculated with strains recommended for culture. In the greenhouse was assessed nodulation and shoot dry weight of cowpea seeds from inoculated and treated with fungicides, and to evaluate the effect of fungicides on germination and seedling development in five cultivars of cowpea. In the course was assessed nodulation and grain yield of cowpea inoculated with strain BR3262 and treated or untreated with fungicides. There was no effect of fungicides on survival of rhizobia on seeds, nor reduce nodulation of plants besides not influence the germination and development of cowpea plants in greenhouse conditions. Under field conditions there was no effect of fungicides on nodulation either on grain yield. With this, the use of fungicides for treating seeds of cowpea and compatible with the inoculation.

Magister Commercii - MCom / The costs of occupational health and well-being are increasingly being considered as sound ‘investments’ as healthy and engaged employees yield direct economic benefits to the company.The concept of Work engagement plays a vital role in this endeavour because engagement entails positive definitions of employee health and promotes the optimal functioning of employees within an organisational setting. The objective of this study was to examine the relationship between work engagement, self-efficacy and optimism amongst call centre employees in a retail organisation in the Western Cape. Over the last several years, most call centre research has predominately been focused on the aspects and causes of stress, burnout, and the deterrents of employee’s well-being. In response to the prevailing preoccupation with negative aspects, the research focused on more positive aspects of human functioning and experiences. The sample comprised of ninety three call centre employees who are employed in the customer service department in a major retail organisation in the Western Cape. Convenience sampling was utilised. The measuring instruments included the Utrecht Work Engagement Scale, The Life Orientation Test-Revised and The General Self-Efficacy Scale. Statistically significant relationships were found between work engagement, self-efficacy and optimism. It was found that call centre agents displayed average levels of work engagement and optimism however they displayed high levels of self-efficacy. It was furthermore found that a moderate percentage of the variance in work engagement can be explained by self-efficacy and optimism. The implication of the results is that interventions that focus on the personal resources (viz. efficacy beliefs,optimism, hope and resiliency) and job resources (viz. physical, social or organizational aspects of the job) will contribute to increasing levels of work engagement.

An assessment of the effect that conventional storage structures, used by smallscale farmers in northern Kwa-Zulu Natal and southern Mozambique, had on germination and vigour of maize seeds was conducted. The survey confirmed that the methods of storing the seed decreased the quality of the maize seeds. Storing maize in the field was good as a short-term solution as initial germination was 100%. Following storage at suboptimum conditions, germination dropped to 25.3%. Commercially treated maize seeds were compared to the test samples collected. After storage, the commercially treated seeds maintained a germination percentage above 75. Untreated maize seeds were treated with fungicides at the recommended dosages. Thereafter the seeds were subjected to germination and vigour tests according to methods outlined by the International Seed Testing Association. All treatments maintained percentage germination above 75. Apron® XL had the highest percentage germination of 83. This trend was also found following the cold test and greenhouse emergence. None of the treatments differed significantly from the control. In this study none of the treatments caused major imbibition damage as indicated by the percentage weight increase and the low leachate conductivity (1012-1271 ìScm-1g-1). The effect of accelerated ageing (AA, 2 and 4 days) and long-term storage (3 and 6 months) on germination and vigour of treated maize seeds was investigated. In the untreated control and treatments there was a gradual decrease in germination following ageing and storage of the seeds. Apron® XL failed to germinate after 3 months. The decrease in germination was mirrored by the leachate conductivity readings. Thiram was the only treatment to maintain germination after 6 months storage. The seeds were planted in two greenhouse trials to assess the performance of the treatments in vivo. The first trial evaluated the emergence and second the emergence and control of Fusarium graminearum. Results from the first trial showed that following 2 d AA, seeds treated with Thiram had the highest percentage emergence (70.7) followed by Celest® XL (68) and the untreated control (62.7). Following inoculation, a similar trend was seen for the treatments and the untreated control. In relation to the percentage seedlings emerged, the control had the highest percentage diseased seedlings. Celest® XL had the lowest percentage diseased seedlings (10, 2 and 1) but failed to germinate after 6 months storage. Thiram was the only treatment to emerge after 6 months storage. The ultrastructural changes in embryonic roots of the untreated control, Celest® XL and Apron® XL were investigated using transmission electron microscopy. These seeds were subjected to 48 hr rapid imbibition and 2 d AA. The most obvious difference between the untreated control, Apron® XL and Celest® XL was the number and position of the vacuoles. In contrast the lipid layer was still attached to the cell wall in the Apron® XL and Celest® XL treatments but in the untreated control they appeared more concentrated in the cytoplasm. This study proved that Thiram was the best treatment among the fungicides tested. However, these results need to be confirmed using a larger range of maize seed lots. / Thesis (PhD)--University of Pretoria, 2011. / Microbiology and Plant Pathology / unrestricted

This research study investigated the effect of job engagement to organisational stress and the employees’ intention to quit among administrative personnel at the University of Fort Hare. The main objectives of this study were determining the effects of organisational stress on employees’ intention to quit, examining the role of job engagement as a moderator of the relationship between organisational stress and employees’ intention to quit, and providing results that would lead to a deeper understanding of the nature and extent of the relationship between organisational stress and intention to quit. A survey method was adopted for this study. A questionnaire comprising of four sections: biographical information, ERI questionnaire for stress, turnover intention, and UWES scale, was administered to non-academic support personnel of the University of Fort Hare in all three campuses namely Alice, Bhisho, and East London. Simple random sampling was used to select the participants. The literature reviewed displayed a great need for managers to understand organisational stress and its causes, and how to manage and cope with stress so as to avoid losing employees, especially highly competent employees. The study yielded results that show that there are interrelationships amongst the three variables, and that job engagement does moderate the relationship between organisational stress and employees’ intention to quit.

Thesis (MScAgric (Viticulture and Oenology))--University of Stellenbosch, 2010. / ENGLISH ABSTRACT: Grapevine water status is considered to be the most important factor limiting plant growth and production in the Mediterranean zones. In these regions with limited summer rainfall and limited water resources for irrigation grapevines may experience water deficits for an extended period of time. The demand of water for agriculture is constantly increasing, and will continue to do so due to the rise in the world population and to the effects of climate change on rainfall and evaporative demand in these regions. The Western Cape wine region is also classified as Mediterranean and grapevines grown in this region are often exposed to water “stress” conditions due to high evaporative demand and low water availability in the soil. Plant water status of grapevines may dependent on, amongst other factors, the water potential of soil layers close to the root system, canopy size and evaporative demand. The canopy size of a grapevine can inherently be seen as a measure of grapevine vigour, and vigour variation among grapevines within a vineyard is a common phenomenon in the Western Cape. The importance of the contributions from several factors causing vigour variation within vineyards is still a subject of debate. This may be largely ascribed to the significant amount of variability in vineyards that researchers have to deal with during viticultural studies. However, the recent advances in remote sensing technology have established new methods to assess grapevine vigour variability. In the face of the recognized variation within vineyards and the importance of a sustained grapevine water status, for wine grape productivity and -quality, it is alarming to think that a vineyard block is generally managed as a homogeneous entity when it comes to irrigation scheduling. What is more alarming is the assumption that grape, juice and wine quality will be homogeneous throughout a vineyard block – even without irrigation. With this in mind, a study was conducted to study the interaction between grapevine vigour and grapevine water status within a commercial vineyard with variable vigour by implementing various irrigation regimes. Vigour variation was identified through multispectral aerial imagery and plant-based water status determinants were used to assess grapevine water status in plots of differing vigour within the vineyard. Soil water status was also assessed, and vegetative growth quantified to ultimately determine the variability in vigour and its possible contribution to the variability through the water status of the plant. Reproductive growth was monitored continually before evaluating the effect of water status and grapevine vigour on grape composition and subsequent wine quality. The various methods used to evaluate grapevine vigour showed good correspondence. Pruning mass measured at the end of the season confirmed leaf area measurement (main leaves and lateral leaves) during vegetative growth, and corresponded well, in terms of main vigour classifications with the NDVI images collected. Berry weight and volume responded to the various classifications, with a decrease in water deficits from one classification to the next accompanying an increase in berry weight and volume. Analyses of the berry composition and wines showed statistically significant differences between the classifications. This was found for sugar content per berry, total phenols, total red pigment, malic acid, nitrogen and pH for the grape juice analyses. Wine pH and total acidity also differed significantly. / AFRIKAANSE OPSOMMING: In die Mediterreense sones word plantwaterstatus beskou as `n hooffaktor wat groei en produksie van `n wingerdstok negatief beinvloed. In hierdie sones kan wingerdstokke vir lang periodes `n tekort aan water ervaar a.g.v `n tekort aan reënwater gedurende die somer en lae beskikbaarheid van besproeingswater. Die vraag na water vir landbou is ook konstant besig om toe te neem in dié sones en die tendens sal voorduur a.g.v die groei in die wêreldbevolking, die effek van klimaatsveranderig op reënvalpatrone en die hoë verdampingsfaktor. Die wingerd- en wynstreek van die Wes-Kaap word ook geklassifiseer as Mediterreens en wingerdstokke in hierdie streek ervaar dikwels waterspanning wat deur hoë evapotranspirasie en min beskikbare grondwater veroorsaak word. Van die faktore wat die waterstatus van `n wingerdstok bepaal is onder andere die waterpotensiaal van die grondlae rondom die wortelstelsel, die grootte van die wingerdlowerraamwerk en die evapotranspirasiebehoefte. Die omvang van `n wingerdstok se lower binne die prieel word beskou as `n aanduiding van wingerdstokgroeikrag en variasie in groeikrag tussen wingerdstokke is `n algemene verskynsel in die Wes-Kaap. Die rangorde, wat die effek van die verskeie faktore wat groeikragvariasie tussen wingerdstokke bepaal, word steeds gedebatteer. Die debat kan groottendeels toegeskryf word aan die beduidende hoeveelheid variasie tussen wingerde waarmee navorsers te doen kry in wingerdkundige studies. Hoewel, met onlangse vordering aangaande afstandswaarnemingstegnologie is daar nou nuwe metodes beskikbaar om wingerdgroeikrag te evalueer. Dit is kommerwekend om te dink dat `n wyndruifwingerd normaalweg as `n homogene eenheid bestuur word as dit kom by besproeiing. Veral met die wete dat groeikragvariasie tussen wingerde algemeen erken en aangeteken word, en dat volhoubare waterstatus van `n wingerdstok van kardinale belang is vir produksie en kwaliteit van wyndruiwe. Die aanname dat wyndruiwe, die sap- en ook wynkwaliteit homogeen sal wees regdeur `n wingerdblok is egter meer kommerwekkend. Na aanvang van dié denke is daar `n studie geloods om die interaksie tussen wingerdstokgroeikrag en wingerdstokwaterstatus te evalueer. Met die studie is verskeie besproeiingsregimes aangebring binne `n kommersiële wingerd wat interne groeikragvariasie tentoonstel. Groeikragvariasie was geïdentifiseer deur middel van multispektrale lugfotos terwyl die wingerdstok se waterstatus geëvalueer is met behulp van plantgebaseerde metings in die verskillende groeikragareas. Die waterstatus van die grond is geëvalueer tesame met die vegetatiewe groei van die wingerd sodat die groeikragvariasie en die invloed van die plantwaterstatus op die groeikrag bepaal kon word. Die reproduktiewe groei is deurlopend gemonitor voor die effek van wingerdstokwaterstatus en wingerdstokgroeikrag op druifsamestelling en wynkwaliteit bepaal is. Daar was `n goeie ooreenkoms tussen die verskeie metodes wat gebruik is om wingerdgroeikrag te bepaal. Snoeimassa aan die einde van die seisoen was ooreenkomstig met die blaaroppervakte (hooflootblare en sylootblare) wat tydens vegetatiewe groei gemeet is, en het ook goed korreleer, met die multispektrale lugfotos se hoof groeikragklassifikasie. Korrelgewig en -volume het reageer op die verskeie besproeiingsregimes, en daar was `n toename in korrelgewig en -volume saam met die afname in watertekort van een regime tot `n ander. Daar was beduidende verskille tussen die verskeie klassifikasies t.o.v. korrelsamestelling analise en wynevaluasie. Die suikerinhoud per korrel, totale fenole, totale rooi pigment, appelsuur, stikstof en pH het verskil in druiwesap analises. Die pH en suur van die wyne het ook beduidend verskil.

Saccharomyces yeasts are ideal candidates for genomic and evolutionary studies in eukaryotes due to their small genome, short generation time and availability of genomic data. Species freely hybridize producing viable but largely sterile cells. A hybridization event can be a swift mechanism for evolutionary innovation that if successful, may produce individuals fitter than either parents. It is largely unclear which mechanisms contribute to such hybrid vigour. This thesis investigated three mechanisms by which a natural hybrid may utilise one or both subgenomes to its advantage: recombination, the formation of chimeric protein complexes and the inheritance of mitochondrial DNA. Three strains of Saccharomyces pastorianus, a natural hybrid of Saccharomyces cerevisiae and Saccharomyces eubayanus, used in the lager fermentation process were sequenced using a NGS SOLiD platform. An analysis of recombination between each subgenome revealed the presence of 30 breakpoints, 28 of which are found within coding regions. Two breakpoints, present within the genes XRN1 and HSP82 have been reused in all three strains of S. pastorianus. This thesis investigated the formation of chimeric protein complexes in S. pastorianus by determining the configuration of protein complex-forming gene pairs to see whether they were mainly uni-specific, with all members belonging to the same parent, or chimeric, comprising one member from each parental species. A total of 21 pairwise protein complexes were found to be obligatorily chimeric in three strains of S. pastorianus. We used PCR-mediated gene deletion to recreate chimeric protein complexes in laboratory hybrids of S. cerevisiae and S. uvarum. The allelic configuration of one protein-complex forming gene pair, MLP2 and SPC110, impacted the growth of hybrid strains in a temperature-dependent manner. Finally, we looked at the mitochondrial inheritance in hybrids. Yeast hybrids can initially inherit mitochondrial DNA (mtDNA) from both parents, but rapidly become homoplasmic. To investigate the mechanisms influencing mtDNA inheritance, strains of Saccharomyces cerevisiae and Saccharomyces uvarum were crossed under different environmental conditions. The majority of hybrids inherited S. cerevisiae mtDNA when crossed in glycerol, a carbon source that can only be respired by yeast, in a range of temperatures. Those crossed in glucose, a fermentable source, did not show a preference for the inheritance of mtDNA at 30°, but at 10°C preferentially inherited S. uvarum mtDNA. In subsequent growth assays, hybrids with S. cerevisiae mtDNA grew better than those with S. uvarum mtDNA at 30°C and 20°C. However, at 10°C, the reverse was true: hybrids with S. uvarum mtDNA grew better that those with S. cerevisiae mtDNA, although only in glycerol. Overall this works sheds light on the molecular mechanisms contributing to fitness and evolutionary vigour in yeast hybrids.

Made available in DSpace on 2016-04-28T14:58:37Z (GMT). No. of bitstreams: 1 2008 - Camila Santos Barros.pdf: 811099 bytes, checksum: 452e8563bd4cfa234b8ac79183a70bb3 (MD5) Previous issue date: 2008-08-15 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / Sunflower production may be influenced by plant density, requiring uniformity in the initial growth phase. This may be influenced by water availability, as well as by soil temperature from germination through emergence. The priming technique has been used to reduce the period between germination and seedling emergence in the filed, providing better performance under stress conditions. Hence, an experiment was performed to evaluate the effect of three seed priming methods in sunflower achenes physiological quality, under different temperatures and water substrate potential. Thus, sunflower, cv. Catissol 01 achenes were submitted to artificial aging at 45?C for 0, 24 and 36 hours, to obtain three different lots. The achenes of each lot were submitted to osmopriming in KNO3 and polyethyleneglycol (PEG) 6000 solution and to hydropriming with subsequent drying. Following, lots were divided into two sub-lots, the first one being immediately evaluated and the second kept under controlled conditions and evaluated two months after storage. Achenes were submitted to germination tests at zero, 15, 35 e 45?C and at zero, -0,3, -0,6 e -0,9 MPa and tests of vigour (first counting, electrical conductivity, accelerated aging, emergence of seedlings and speed of seedlings). Results indicated that osmopriming with PEG solution after two month storage, improved germination and vigor, measured by first counting test, of lots aged for 24 hours under -0.3 MPa. Osmopriming with KNO3 solution also improved germination and vigour of the unaged lot, at the first counting test under 15?C, on both evaluations. Under hydropriming, vigour increase, measured by accelerated aging test, was only found in 36 hour artificially aged lots. During storage, maintenance of germination under 15?C on the lot achenes artificially aged for 36 hours, under 25?C on the the unaged lot and under under -0.3 MPa on the unaged lot occurred after osmopriming with PEG solution . Maintenance of germination under -0.3 MPa on the unaged lot after osmopriming with KNO3 solution also occurred. / A produ??o de girassol pode ser influenciada pela densidade de plantas, sendo importante a uniformidade da popula??o inicial de plantas, a qual pode ser afetado tanto pelo restri??o do potencial h?drico como pela temperatura do solo, desde a fase de germina??o at? a emerg?ncia de pl?ntulas. A t?cnica de condicionamento fisiol?gico tem sido utilizada visando diminuir o per?odo entre a germina??o e a emerg?ncia de pl?ntulas a campo, bem como proporcionar acentuada performance sob condi??es de estresse. Dentro deste contexto, foi instalado um experimento com o objetivo de avaliar o efeito de tr?s m?todos de condicionamento fisiol?gico na qualidade fisiol?gica dos aqu?nios de girassol, sob diferentes temperaturas e potencias h?dricos do substrato. Para isso, foram utilizados aqu?nios da variedade Catissol 01, que foram submetidos ao envelhecimento artificial a 45?C durante 0, 24 e 36 horas, para obten??o de tr?s distintos lotes. Os aqu?nios de cada lote, foram submetidos ao osmocondicionamento usando solu??o de KNO3 e de polietilenoglicol (PEG) 6000 e ao hidrocondicionamento, com subsequente secagem. Posteriormente, os lotes foram divididos em dois sublotes, sendo um avaliado imediatamente e, o outro, mantido em condi??es controladas e avaliado ap?s dois meses de armazenamento. Os aqu?nios foram submetidos aos testes de germina??o sob zero, -0,3, -0,6 e -0,9 MPa e sob 15, 25, 35 e 45?C e de vigor (primeira contagem, condutividade el?trica, envelhecimento acelerado, emerg?ncia de pl?ntulas e ?ndice de velocidade de pl?ntulas). Pelos resultados pode-se concluir que o osmocondicionamento com solu??o de PEG, ap?s o armazenamento, favorece a germina??o e o vigor, considerando o teste de primeira contagem sob -0,3 MPa do lote de aqu?nios envelhecido por 24 horas. O osmocondicionamento com solu??o de KNO3, em ambas as avalia??es, promove a germina??o e o vigor, estimado pelo teste de primeira contagem sob 15?C, do lote de aqu?nios n?o envelhecido. Ap?s o hidrocondicionamento, h? favorecimento somente do vigor, avaliado pelo teste de envelhecimento acelerado, do lote de aqu?nios envelhecidos por 36 horas. Durante o armazenamento, houve manuten??o da germina??o sob 15?C, ap?s o osmocondicionamento com solu??o de PEG do lote de aqu?nios envelhecidos por 36 horas, sob 25?C do lote n?o envelhecido, e da germina??o sob -0,3 MPa, ap?s o osmocondicionamento com solu??o de KNO3 do lote de aqu?nios n?o envelhecido.

In order to be sustainable, companies have to adapt in the ever–changing market and economic conditions which are often unpredictable. The adaptation to these challenges rests with employees who have to stay motivated and psychologically well. The environmental working conditions are often undesirable The objective of this study was to evaluate the psychometric performance of the Utrecht Work Engagement Scale (UWES) and the Oldenburg Burnout inventory (OLBI) within the cement factory. A random sampling approach was adopted by distributing a questionnaire for the purposes of achieving the general research objective with an availability sample (N = 187). The UWES and OLBI were administered. Exploratory factor analysis, descriptive statistics, Cronbach alpha coefficients, Pearson product–moment correlations and MANOVA's were used to analyse the data. The outcome of the study through literature review confirmed that work engagement and burnout are two important components of employee wellbeing. The factor loadings of the UWES and OLBI resulted in a two–factor structure for both the UWES and OLBI. The two factor structure for the UWES were labelled as Vigour/Dedication and Absorption. The twofactor structure for the OLBI was labelled Disengagement and Exhaustion. A small number of items were retained for the OLBI. The internal consistency of the UWES was found to be well above the acceptable level with the alpha coefficients exceeding 0,70. The internal consistency for the OLBI was found to be lower than the 0,70 level. Data analysis further showed that correlations between engagement and burnout were statistically insignificant. Data analysis also showed that there were no vi significant differences for age and gender for both engagement and burnout, however there is a significant difference in race and language with regard to engagement but none for burnout. Limitations within the study were identified and recommendations for future research were made. / Thesis (M.A. (Industrial Psychology))--North-West University, Potchefstroom Campus, 2011.

In order to be sustainable, companies have to adapt in the ever–changing market and economic conditions which are often unpredictable. The adaptation to these challenges rests with employees who have to stay motivated and psychologically well. The environmental working conditions are often undesirable The objective of this study was to evaluate the psychometric performance of the Utrecht Work Engagement Scale (UWES) and the Oldenburg Burnout inventory (OLBI) within the cement factory. A random sampling approach was adopted by distributing a questionnaire for the purposes of achieving the general research objective with an availability sample (N = 187). The UWES and OLBI were administered. Exploratory factor analysis, descriptive statistics, Cronbach alpha coefficients, Pearson product–moment correlations and MANOVA's were used to analyse the data. The outcome of the study through literature review confirmed that work engagement and burnout are two important components of employee wellbeing. The factor loadings of the UWES and OLBI resulted in a two–factor structure for both the UWES and OLBI. The two factor structure for the UWES were labelled as Vigour/Dedication and Absorption. The twofactor structure for the OLBI was labelled Disengagement and Exhaustion. A small number of items were retained for the OLBI. The internal consistency of the UWES was found to be well above the acceptable level with the alpha coefficients exceeding 0,70. The internal consistency for the OLBI was found to be lower than the 0,70 level. Data analysis further showed that correlations between engagement and burnout were statistically insignificant. Data analysis also showed that there were no vi significant differences for age and gender for both engagement and burnout, however there is a significant difference in race and language with regard to engagement but none for burnout. Limitations within the study were identified and recommendations for future research were made. / Thesis (M.A. (Industrial Psychology))--North-West University, Potchefstroom Campus, 2011.

Orientador: Roberval Daiton Vieira / Banca: Rubens Sader / Banca: Ana Dionísia da Luz Coelho Novembre / Resumo: A agricultura moderna exige testes rápidos para avaliação da qualidade de sementes para determinar o seu uso potencial. O teste de condutividade elétrica é usado para sementes de muitas espécies, especialmente as de leguminosas. O objetivo dessa pesquisa foi o de avaliar o efeito da temperatura de armazenamento sobre os resultados do teste de condutividade elétrica e da composição mineral da solução de embebição de sementes de feijão (Phaseolus vulgaris L.) armazenadas durante um ano a temperatura de 10 e 25 °C. Foram utilizados três lotes da cultivar IAPAR - 81 (grupo carioca) e três lotes da cultivar IPR - TIZIU (grupo preto) armazenadas em câmara fria a 10 °C e em BOD a 25 °C. As avaliações foram reali zadas em intervalos de três meses ao longo de 12 meses e as variáveis avaliadas foram: teor de água, germinação e vigor (envelhecimento acelerado e condutividade elétrica). O conteúdo de potássio da solução de embebição foi determinado por fotometria de chama e o conteúdo de cálcio e magnésio por espectrofotometria de absorção atômica. Os dados foram analisados segundo o delineamento inteiramente casualizado em esquema fatorial (4 períodos de armazenamento x 3 temperaturas) com tratamento adicional (avaliação inicial) com quatro repetições para cada lote. As sementes das cultivares apresentaram comportamento semelhante no armazenamento. / Abstract: Modern agriculture requires quick tests to evaluate the seeds quality, in order to determine their potential use. The electrical conductivity test has been used for many species, especially legumes seeds. This research was carried out in order to investigate the relationship between the amount of electrolytes (K+, Ca++ and Mg++) on the seed soaked solution and the vigor measured by electrical conductivity test of bean seeds (Phaseolus vulgaris L.) stored at 10 and 25 °C. Three seed lots cultiv ar IAPAR - 81 (Carioca Group) and three cultivar IPR - TIZIU (black group) were stored in a cold chamber at 10 °C and in a BOD at 25 °C. Eval uations were done in intervals of three months over a 12 months and the variables were: water content, germination and vigor (accelerated aging and electrical conductivity tests). The potassium content in the imbibition solution was determined by flame photometry and the calcium and magnesium contents, by atomic absorption spectrophotometry. The statistics model used was completely randomized design in a factorial (four periods of storage X three temperatures) with additional treatment (initial assessment) with four replicates for each lot. The cultivars showed similar behavior in storage. Electrical conductivity values decreased in the last evaluation and it was not followed by reduction of the ions quantified. The potassium was the ion leached in larger amounts from bean seeds stored, and it's behavior was closest to the results of electrical conductivity test. / Mestre