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Thesis (M.S.)--West Virginia University, 2009. / Title from document title page. Document formatted into pages; contains vii, 51 p. : ill. (some col.). Includes abstract. Includes bibliographical references (p. 46-51).

A ecotoxicologia emergiu como uma necessidade de avaliar e mitigar os impactos antrópicos no ambiente natural e nas comunidades viventes no local, utilizando uma gama de experimentos em campo e em laboratório. Dentre as diversas áreas estudadas a reprodução é um dos processos que podem ter seu desempenho diminuído devido à ação antrópica, sendo assim importante seu estudo na ecotoxicologia. O objetivo deste trabalho foi avaliar a influência do ambiente impactado nos processos fisiológicos associados à maturação oocitária em fêmeas de Astyanax fasciatus. Fêmeas de A. fasciatus foram coletadas entre 2012 e 2014 e machos (como indicadores da presença de compostos estrogênicos) entre 2013 a 2014, com duas coletas a cada estação do ano em dois reservatórios, Ponte Nova (referência) e Billings (impactado). Amostras de água e sedimento foram também coletadas nos mesmos pontos para análise dos parâmetros físicos e químicos. Estes resultados foram comparados com a resolução CONAMA/357 e calculado o coeficiente de risco. Para ambos os sexos foram analisadas a expressão gênica da vitelogenina e do receptor de estradiol, a concentração plasmática de vitelogenina e estradiol. Foram também realizadas as análises de fecundidade relativa e absoluta, histologia das gônadas, assim como o cálculo dos índices gonadossomático e hepatossomático, apenas nas fêmeas. O coeficiente de risco mostrou que o reservatório Billings apresenta um alto risco para os metais e dos compostos orgânicos. Foram observados valores elevados na concentração plasmática de estradiol e expressão de vitelogenina em machos deste reservatório, na primavera de 2013, assim como foram observados valores mais elevados de fecundidade relativa e absoluta nas fêmeas, no inverno e primavera deste mesmo ano, em comparação com os animais do reservatório referência (Ponte Nova). Esses resultados sugerem que no inverno e primavera de 2013, os poluentes como os aqui analisados e outros aqui não detectados, podem ter interferido na reprodução em A. fasciatus do reservatório Billings. No entanto, é necessário destacar que essas alterações podem, em dado momento, prejudicar o processo de maturação oocitária, sendo então importante monitorar e realizar mais estudos de análise de risco ambiental no reservatório / The ecotoxicology has arisen as a need to evaluate and mitigate the anthropic impacts in wild environmental and in the local communities, using several bioassays and wild experiments. Among the several studied areas, the reproduction is a process that can have its performance reduced due to the anthropic impacts, showing the importance of studying these processes in ecotoxicologydy. The aim of this study was to evaluate the influence of the impaired environmental in the physiological processes associated with oocyte maturation of Astyanax fasciatus females. Astyanax fasciatus females were sampled between 2012 and 2014 and males (as indicators of the presence of estrogenic compounds) from 2013 to 2014, with two samplings at each season, in two reservoirs, Ponte Nova (reference) and Billings (impacted). Sediments and water were sampled in the same sites to evaluate the chemical and physical parameters. The results of the analysis were compared with the CONAMA/357 guidelines and the hazard risk was calculated. The gene expression of vitellogenin and estradiol receptor, vitellogenin and estradiol plasma level were performed in males and females. The relative and absolute fecundity, gonad histology, gonadosomatic and hepatosomatic index were evaluated only in females. The hazard risk at Billings reservoir was high for organic compounds and metals. Estradiol levels and vitellogenin gene expression were higher in males sampled in Billings in the spring/2013, as well as the relative and absolute fecundity, that were higher in the winter and spring of the same year, when compared with the animals from the reference site (Ponte Nova). These outcomes suggest that during winter and spring of 2013 the pollutants, as analyzed in this study, as well as others that were not detected, may have interfered in the reproductive process of A. fasciatus at Billings reservoir. However, it is necessary to observe that these alterations might, at a given time, impair the oocyte maturation process, being important to monitor and accomplish additional studies of the environmental risk assessment at Billings reservoir

A ecotoxicologia emergiu como uma necessidade de avaliar e mitigar os impactos antrópicos no ambiente natural e nas comunidades viventes no local, utilizando uma gama de experimentos em campo e em laboratório. Dentre as diversas áreas estudadas a reprodução é um dos processos que podem ter seu desempenho diminuído devido à ação antrópica, sendo assim importante seu estudo na ecotoxicologia. O objetivo deste trabalho foi avaliar a influência do ambiente impactado nos processos fisiológicos associados à maturação oocitária em fêmeas de Astyanax fasciatus. Fêmeas de A. fasciatus foram coletadas entre 2012 e 2014 e machos (como indicadores da presença de compostos estrogênicos) entre 2013 a 2014, com duas coletas a cada estação do ano em dois reservatórios, Ponte Nova (referência) e Billings (impactado). Amostras de água e sedimento foram também coletadas nos mesmos pontos para análise dos parâmetros físicos e químicos. Estes resultados foram comparados com a resolução CONAMA/357 e calculado o coeficiente de risco. Para ambos os sexos foram analisadas a expressão gênica da vitelogenina e do receptor de estradiol, a concentração plasmática de vitelogenina e estradiol. Foram também realizadas as análises de fecundidade relativa e absoluta, histologia das gônadas, assim como o cálculo dos índices gonadossomático e hepatossomático, apenas nas fêmeas. O coeficiente de risco mostrou que o reservatório Billings apresenta um alto risco para os metais e dos compostos orgânicos. Foram observados valores elevados na concentração plasmática de estradiol e expressão de vitelogenina em machos deste reservatório, na primavera de 2013, assim como foram observados valores mais elevados de fecundidade relativa e absoluta nas fêmeas, no inverno e primavera deste mesmo ano, em comparação com os animais do reservatório referência (Ponte Nova). Esses resultados sugerem que no inverno e primavera de 2013, os poluentes como os aqui analisados e outros aqui não detectados, podem ter interferido na reprodução em A. fasciatus do reservatório Billings. No entanto, é necessário destacar que essas alterações podem, em dado momento, prejudicar o processo de maturação oocitária, sendo então importante monitorar e realizar mais estudos de análise de risco ambiental no reservatório / The ecotoxicology has arisen as a need to evaluate and mitigate the anthropic impacts in wild environmental and in the local communities, using several bioassays and wild experiments. Among the several studied areas, the reproduction is a process that can have its performance reduced due to the anthropic impacts, showing the importance of studying these processes in ecotoxicologydy. The aim of this study was to evaluate the influence of the impaired environmental in the physiological processes associated with oocyte maturation of Astyanax fasciatus females. Astyanax fasciatus females were sampled between 2012 and 2014 and males (as indicators of the presence of estrogenic compounds) from 2013 to 2014, with two samplings at each season, in two reservoirs, Ponte Nova (reference) and Billings (impacted). Sediments and water were sampled in the same sites to evaluate the chemical and physical parameters. The results of the analysis were compared with the CONAMA/357 guidelines and the hazard risk was calculated. The gene expression of vitellogenin and estradiol receptor, vitellogenin and estradiol plasma level were performed in males and females. The relative and absolute fecundity, gonad histology, gonadosomatic and hepatosomatic index were evaluated only in females. The hazard risk at Billings reservoir was high for organic compounds and metals. Estradiol levels and vitellogenin gene expression were higher in males sampled in Billings in the spring/2013, as well as the relative and absolute fecundity, that were higher in the winter and spring of the same year, when compared with the animals from the reference site (Ponte Nova). These outcomes suggest that during winter and spring of 2013 the pollutants, as analyzed in this study, as well as others that were not detected, may have interfered in the reproductive process of A. fasciatus at Billings reservoir. However, it is necessary to observe that these alterations might, at a given time, impair the oocyte maturation process, being important to monitor and accomplish additional studies of the environmental risk assessment at Billings reservoir

Teleosts are one of the most diverse groups of vertebrates. They utilize a wide array of reproductive strategies and tactics to overcome the challenges of the many ecological niches they inhabit. The most common reproductive method for teleosts is oviparity. Oviparous animals lay eggs with little or no embryonic development from the mother. The embryos are supplied with nutrition via yolk. Vitellogenesis is the process of the ovary sequestering yolk. It is regulated by exogenous environmental cues that act on the hypothalamus-pituitary-gonad axis. Through a series of hormonal controls, the liver produces the yolk precursor, vitellogenin. Vitellogenin is secreted by the liver and absorbed by the growing oocyte by receptor mediated endocytosis. There it is cleaved into the two main yolk proteins which are subsequently used by the growing embryo. The biggest source of nutrition is the yolk protein lipovitellin which also plays a key role in marine teleosts’ ability to osmoregulate their eggs. Lipovitellin is a large glyco-phospho-lipo-protein ca. 200 kDa. Large proteins usually denature easily. However, prior evidence shows that fish lipovitellins are thermally stable. Using differential scanning calorimetry, I quantify lipovitellin’s thermostability amongst four right-eye flounders (Pleuronectidae: winter flounder, American plaice, witch flounder, and yellowtail flounder). Differential scanning calorimetry allows direct interpretation of all thermodynamic properties; however, Lipovitellin was too large and precipitated before other thermodynamic properties could be determined. Pleuronectid lipovitellins all showed high melting points indicative of high thermostability. This shows that despite differing life histories, lipovitellin is conserved. Presence of the pre-cursor, vitellogenin in male or juvenile fish is used as a biomarker for xenoestrogens, a type of endocrine disrupting chemicals that blocks or mimics natural estrogens. They are known to disrupt aquatic life by interfering with natural development and reproduction. A major biological side effect of xenoestrogens is the accumulation of vitellogenin. This effect has made vitellogenin a useful biomarker for monitoring levels of contamination. Unfortunately, vitellogenin can vary greatly in its immunological and structural characteristics, which means that species-specific assays are necessary. This study took the first step in developing an immunoassay for bluefish (Pomatomus saltatrix). Vitellogenin was induced by injecting a group of bluefish with an estrogen, estradiol, and the resulting vitellogenin was isolated from the serum of males. The protein was characterized as vitellogenin by determining its large Stokes radius in gel permeation chromatography combined with its characteristic peptide molecular weight in sodium dodecyl sulfate polyacrylamide gel electrophoresis.

Pang Yee Man Flora. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2002. / Includes bibliographical references (leaves 111-128). / Abstracts in English and Chinese. / Abstract (in English) --- p.ii / Abstract (in Chinese) --- p.iv / Acknowledgement --- p.v / Table of Contents --- p.vii / List of Figures --- p.xii / Symbols and Abbreviations --- p.xv / Scientific Names --- p.xvii / Chapter Chapter 1 --- General Introduction --- p.1 / Chapter 1.1 --- Vitellogenesis --- p.2 / Chapter 1.2 --- Vitellogenin --- p.3 / Chapter 1.2.1 --- Structure --- p.3 / Chapter 1.2.2 --- Vitellogenin synthesis in the liver --- p.4 / Chapter 1.3 --- Regulation of vitellogenin synthesis --- p.5 / Chapter 1.3.1 --- Estradiol --- p.5 / Chapter 1.3.1.1 --- Mechanism of action --- p.6 / Chapter 1.3.1.2 --- Estradiol-stimulated vitellogenin expression --- p.7 / Chapter 1.3.1.3 --- Memory effects --- p.9 / Chapter 1.3.2 --- Testosterone --- p.10 / Chapter 1.3.3 --- Cortisol --- p.13 / Chapter 1.3.4 --- Progesterone --- p.14 / Chapter 1.3.5 --- Growth Hormone --- p.14 / Chapter 1.3.6 --- Prolactin --- p.15 / Chapter 1.3.7 --- Thyroid hormone --- p.15 / Chapter 1.4 --- Growth factors --- p.16 / Chapter 1.4.1 --- Activin --- p.16 / Chapter 1.4.1.1 --- Structure --- p.16 / Chapter 1.4.1.2 --- Functions --- p.17 / Chapter 1.4.2 --- Epidermal growth factors (EGF) --- p.18 / Chapter 1.4.2.1 --- Structure --- p.18 / Chapter 1.4.2.2 --- Functions --- p.19 / Chapter 1.5 --- Objectives of the present study --- p.20 / Chapter Chapter 2 --- Expression of Goldfish Vitellogenin in vivo and in vitro --- p.25 / Chapter 2.1 --- Introduction --- p.25 / Chapter 2.2 --- Materials and Methods --- p.26 / Chapter 2.2.1 --- Materials --- p.26 / Chapter 2.2.2 --- Sequencing --- p.27 / Chapter 2.2.3 --- Cell culture --- p.28 / Chapter 2.2.4 --- RNA extraction --- p.29 / Chapter 2.2.5 --- Northern hybridization --- p.31 / Chapter 2.2.6 --- Slot blot hybridization --- p.32 / Chapter 2.2.7 --- Data analysis --- p.33 / Chapter 2.2.8 --- SDS-PAGE analysis --- p.33 / Chapter 2.2.9 --- in situ hybridization --- p.34 / Chapter 2.3 --- Results --- p.37 / Chapter 2.3.1 --- Validation of vitellogenin mRNA detection --- p.37 / Chapter 2.3.2 --- Basal and estradiol-stimulated vitellogenin expression and production invivo --- p.38 / Chapter 2.3.3 --- Localization of vitellogenin expression in the liver --- p.39 / Chapter 2.3.4 --- Expression of vitellogenin in vitro --- p.40 / Chapter 2.4 --- Discussion --- p.54 / Chapter Chapter 3 --- Effects of Steroids on the Expression of Goldfish Vitellogenin in vitro --- p.60 / Chapter 3.1 --- Introduction --- p.60 / Chapter 3.2 --- Materials and Methods --- p.62 / Chapter 3.2.1 --- Materials --- p.62 / Chapter 3.2.2 --- Animal --- p.62 / Chapter 3.2.3 --- Primary culture of dispersed hepatic cells --- p.62 / Chapter 3.2.4 --- Drug treatment --- p.64 / Chapter 3.2.5 --- Total RNA isolation --- p.64 / Chapter 3.2.6 --- Messenger RNA isolation --- p.65 / Chapter 3.2.7 --- Slot blot analysis --- p.66 / Chapter 3.2.8 --- Data analysis --- p.68 / Chapter 3.2.9 --- Reverse transcription-polymerase chain reaction (RT-PCR) --- p.68 / Chapter 3.2.10 --- Cloning of aromatase cDNA --- p.69 / Chapter 3.2.11 --- Sequencing --- p.70 / Chapter 3.3 --- Results --- p.71 / Chapter 3.3.1 --- Effect of 17-β estradiol on vitellogenin mRNA expression --- p.71 / Chapter 3.3.2 --- Effect of testosterone on vitellogenin mRNA expression --- p.71 / Chapter 3.3.3 --- Detection of aromatase mRNA expression in the liver by RT-PCR --- p.72 / Chapter 3.3.4 --- Effect of aromatase inhibitors on testosterone-stimulated vitellogenin expression --- p.73 / Chapter 3.4 --- Discussion --- p.81 / Chapter Chapter 4 --- Effects of Epidermal Growth Factor (EGF) and Activin on the Expression of Vitellogenin in the Goldfish Hepatic Cells in vitro --- p.86 / Chapter 4.1 --- Introduction --- p.86 / Chapter 4.2 --- Materials and Methods --- p.88 / Chapter 4.2.1 --- Materials --- p.88 / Chapter 4.2.2 --- Primary culture of dispersed hepatic cells --- p.89 / Chapter 4.2.3 --- Slot blot analysis --- p.91 / Chapter 4.2.4 --- Data analysis --- p.91 / Chapter 4.3 --- Results --- p.92 / Chapter 4.3.1 --- Effect of activin on vitellogenin mRNA expression --- p.92 / Chapter 4.3.2 --- Effect of EGF and TGF-α on vitellogenin mRNA expression --- p.93 / Chapter 4.4 --- Discussion --- p.99 / Chapter Chapter 5 --- General Discussion --- p.104 / Chapter 5.1 --- Overview --- p.104 / Chapter 5.2 --- Contribution of the present study --- p.106 / Chapter 5.2.1 --- Expression of goldfish vitellogenin in vivo and in vitro --- p.106 / Chapter 5.2.2 --- Effects of steroids on the expression of goldfish vitellogenin in vitro --- p.106 / Chapter 5.2.3 --- Effects of EGF and activin on the expression of vitellogenin in the goldfish hepatic cells in vitro --- p.107 / Chapter 5.3 --- Future prospects --- p.108

Methoprene is a Juvenile Hormone (JH) analogue commonly used for the control of mosquito larvae. When applied to a mosquito breeding site, methoprene enters the haemolymph, where it mimics the function of JH and interferes with normal metamorphosis, resulting in larval mortality. Methoprene is commonly used for the control of larvae and has not been used as an adulticide, due to an absence of acute effects. This study evaluated possible chronic effects caused by the exposure of adult Aedes mosquitoes to methoprene. Methoprene was applied, in both technical grade and the commercially available Altosid®, topically to adults through droplet application on the abdomen and as a spray application. Mosquitoes were examined for treatment effects on ovary development, adult male and female mortality, and fecundity. The results demonstrate that relatively high doses are required to affect adult survivorship. In contrast, significant impacts on both fecundity and egg hatch were observed for females treated at the lower dosages. I discuss the results in relation to autocidal strategies for mosquito control in which the release of fecund females is to be avoided.

Orientador: Alexandre Ninhaus Silveira / Resumo: O objetivo desse trabalho foi investigar a possível associação de variáveis ambientais, como o fotoperíodo, a concentração de oxigênio dissolvido, a temperatura da água, o pH, a condutividade, a transparência e a precipitação pluviométrica no ciclo reprodutivo anual de fêmeas de Brycon amazonicus mantidas em cativeiro. Para tal, foi avaliada a variação da concentração plasmática do Estradiol (E2) durante 1 ciclo reprodutivo e correlacionado com o conjunto das variáveis acima citadas. O experimento foi conduzido no Centro Nacional de Pesquisa e Conservação de Peixes Continentais (CEPTA/ICMBio), Pirassununga, Estado de São Paulo, entre os meses de maio de 2014 a abril de 2015. Para tal, foram utilizadas treze fêmeas microchipadas de Brycon amazonicus com 4 anos, massa de 910±142.97g e comprimento total de 39.79±1.83cm. Esses exemplares foram alimentados com ração extrusada, com 24% de proteína bruta, a uma taxa de 5%peso vivo/dia. As amostras de sangue foram coletadas mensalmente, sendo os reprodutores previamente anestesiados em solução de Fenoxyetanol (0,4mL/L de água - Sigma Aldrich®). As amostras de plasma foram analisadas por ensaio imunoenzimático. Diariamente foram coletados os parâmetros de Oxigênio Dissolvido, pH, Transparência, Condutividade, Temperatura, Fotoperíodo e Precipitação Pluviométrica. Foi aplicada a análise exploratória multivariada de dados pelo método da Análise de Componentes Principais (PCA) com representação gráfica via Biplot, e uma vez que o conjunt... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: The aim of this study was to investigate a possible association of environmental variables such as photoperiod, dissolved oxygen, water temperature, pH, conductivity, transparency and pluviosity, along the reproductive cycle of Brycon amazonicus females kept in captivity. For that, were correlated during one reproductive cycle, variations of plasmatic Estradiol (E2) concentration and all environment variables above. The experiment was conducted in CEPTA/ICMBio, Pirassununga, São Paulo State, from May 2014 to April 2015. Thirteen tagged females of Brycon amazonicus with four years olds were used, weighing 910 ± 142.97g with total length of 39.79±1.83cm. These specimens were fed by extruded feed with 24% crude protein, using a ratio of 5% body weight. Blood sample were monthly collected and breeders were previously anesthetized by Fenoxyetanol (0,4mL/L-Sigma Aldrich®) before each sampling. Plasmatic samples were analyzed by enzyme-linked immunosorbent assay. During all trial were recorded daily variables of Dissolved Oxygen, pH, Transparency, Conductivity, Temperature, Photoperiod and Pluviosity. A multivariate exploratory data analysis was applied by Principal Component Analysis (PCA) method and graphically represented via Biplot. As data were not normally distributed (Shapiro-Wilk Test), it was applied Spearman Correlation and Kruskal–Wallis test supported by Software R (Package Spearman and Prcomp). Principal Component Analysis results suggests a possible positive correlatio... (Complete abstract click electronic access below) / Mestre

Submitted by (lucia.rodrigues@ufrpe.br) on 2016-05-23T13:46:00Z No. of bitstreams: 1 Antonio Pedro Soares.pdf: 1816164 bytes, checksum: c9d36edf08ffef683ecc8bd6879cff2b (MD5) / Made available in DSpace on 2016-05-23T13:46:00Z (GMT). No. of bitstreams: 1 Antonio Pedro Soares.pdf: 1816164 bytes, checksum: c9d36edf08ffef683ecc8bd6879cff2b (MD5) Previous issue date: 2010-02-25 / This study aimed to characterize histologically the cycle of vitellogenesis of painted lobster Panulirus echinatus from Southern Coast of Pernambuco, in different stages of maturation. 173 females animals were collected from for a period of 12 months between the months of December 2007 to November 2008, an average of 15 animals per month, Tamandaré Beach, South Coast of Pernambuco State. Data collection was performed using gillnets and the samples were taken at random. After being captured the animals were euthanized by heat shock, ovaries and hepatopancreas were collected and individually weighed, fixed in 10% formaldehyde in water from the site of capture for paraffin and the 4% buffered glutaraldehyde for inclusion in historesine. The blocks were cuted and stained with hematoxylin-eosin, Gomori's trichrome, hematoxylin / phloxine, PAS + hematoxylin, Control, PAS, PAS + Alcian Blue pH 2.5 and Sudan Black. Histological analysis revealed four maturation stages: development (stage II) Pre-maturity (stage III), maturation (stage IV) and post-spawning (stage V) and three-stage oocytes, no vitellogenic, vitellogenic primary phase, and secondary vitellogenesis. In mature oocytes the cytoplasm is PAS positive and presence of lipid droplets positive for Sudan. Gonadossomatic and hepatossomatic indexes showed an inverse correlation in the different stages of maturation indicating a possibility of transfer of yolk from hepatopancreas to the ovaries during the reproductive period. / Este trabalho objetivou caracterizar histologicamente o ciclo de vitelogênese da lagosta pintada Panulirus echinatus do litoral sul de Pernambuco, nos diferentes estágios de maturação. Foram coletados 173 animais fêmeas por um período de 12 meses entre os meses de dezembro de 2007 a novembro de 2008, uma média de 15 animais por mês, na Praia de Tamandaré, Litoral Sul do Estado de Pernambuco. A coleta foi realizada utilizando-se rede de espera e as amostras foram obtidas de forma aleatória. Após serem capturados os animais foram ortonasiados por choque térmico, ovários e hepatopâncreas foram coletados e pesados individualmente, fixados em formaldeido a 10% em água do local da captura para inclusão em parafina e em glutaraldeído à 4% tamponado para inclusão em historresina . Os blocos foram cortados e corados pela Hematoxilina/Eosina, Tricrômico de Gomori, Hematoxilina/Floxina, PAS+Hematoxilina, Controle PAS, PAS+Alcian Blue pH 2.5 e Sudan Black. As análises histológicas revelaram quatro estágios de maturação: Desenvolvimento (estágio II); Pré-maturação (estágio III); Maturação (estágio IV) e Pós-desova (estágio V) e três fases ovocitárias: não vitelogênica, fase de vitelogênese primária, e vitelogênese secundária . Nos ovócitos maduros o citoplasma apresentou-se PAS positivo e presença de gotículas de lipídios positivas ao Sudan. Os índices gonodossomáticos e hepatossomáticos demonstraram uma correlação inversa nos diferentes estágios de maturação gonadal indicando uma possibilidade de transferência de vitelo do hepatopâncreas para os ovários durante o período reprodutivo.

Orientador: Arício Xavier Linhares / Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia / Made available in DSpace on 2018-08-25T23:06:38Z (GMT). No. of bitstreams: 1 Ferraz_DaniloRibeiro_M.pdf: 2070808 bytes, checksum: 257105fe3aaa84a8abe9acf46006298a (MD5) Previous issue date: 2014 / Resumo: Os insetos apresentam importância do ponto de vista forense, possibilitando coletar evidências e informações essenciais à justiça. Para o aprimoramento das aplicações da entomologia forense é de extrema importância que sejam realizados estudos relacionados a fisiologia, taxonomia, ecologia, comportamento e distribuição da entomofauna decompositora. Dentre os insetos necrófagos, califorídeos (Diptera) apresentam grande destaque, pois produtos de origem animal são substratos para oviposição e fonte de proteínas e carboidratos para adultos, além de constituir recurso alimentar para larvas de algumas espécies. Os califorídeos passam por diferentes fases de desenvolvimento ovariano sendo que, nos estágios intermediários de desenvolvimento, há intensa deposição de vitelo pelas células tróficas. Assim, com fim de contribuir para um maior entendimento fisiológico e comportamental da família, o presente estudo objetiva avaliar e comparar sua riqueza, observando o padrão de atratividade exercido sobre moscas varejeiras por três diferentes substratos de origem animal (frango, peixe e roedor), bem como os estágios de desenvolvimento ovariano no qual os espécimes coletados se encontravam. Ao todo foram coletados 12.332 espécimes, dos quais 1.432 pertenciam a Calliphoridae. As iscas de moela de frango foram mais atrativas a ordem Diptera, ao passo que a família Calliphoridae foi mais atraída por iscas de roedor. Neste trabalho, 803 fêmeas foram dissecadas, sendo que grande parte destas fêmeas encontravam-se em estágio de vitelogênese completa demostrando a importância da proteína animal como substratos para oviposição / Abstract: Insects are of great forensic importance because they can serve as evidence and provide essential information to justice. In order to improve the applications of forensic entomology, it is of great important to study the biology, ecology, physiology, taxonomy, behavior and distribution of insect fauna associated to decomposing animal matter. Among the scavenger insects, the calliphorids (Diptera) are very important, because many species utilize animal tissues as source of protein and for immature development. Baits of animal origin can act as substrat for oviposition and as source of proteins and carbohydrates for the adults and for larval development feeding .Ovaries of calliphorids undergo different ovarian development phases from the pre-viteloogenic stage to mature eggs. Intermediate phases are characterized by deposition of yolk by the nurse cells. Thus, this study, aimed to contribute to the understanding of the physiological and behavioral characteristics of species of calliphorids, presenting and comparing species richness, attractiveness patterns of three different baits of animal origin these flies (chicken gizzard, fish and rat) as well as the stages of ovarian development in which the collected specimens were found. In all, 12,332 specimens were collected, of which 1,432 belonged to Calliphoridae. The rodent bait was the most attractive to this family while chicken gizzard was more attractive to Diptera as a whole. In this work, 803 females were dissected. The majority of these were in the stage of mature egg, indicating the use of the substracts for oviposition, although this pattern changed depending on the species analyzed / Mestrado / Biodiversidade Animal / Mestre em Biologia Animal