The host microbiome (mainly bacteria) is essential for host immune function, metabolism, and digestion. Alterations in these microbes, known as dysbiosis, generally results in adverse effects to the host, including diseases. Dysbiosis can be induced from exposures to various anthropogenic contaminants including constituents of municipal wastewater treatment effluents (MWWEs), namely, pharmaceuticals, antibiotics, and excess nutrients. Despite MWWEs being one of the largest dischargers to aquatic ecosystems, impacts of these contaminants on exposed organism microbiomes, especially in aquatic insects, is unclear. In addition, some aquatic contaminants may transfer to riparian habitats through predation on emergent insects that were exposed to contaminants as larvae, and subsequently alter microbiomes of terrestrial predators. Our study evaluated whether MWWEs altered microbiomes of freshwater larval and adult insects and their riparian spider predators using effluent-associated bacteria and stable nitrogen isotopes (δ15N) to confirm effluent exposure. We analyzed microbiome compositions through sequencing of the V3-V4 hypervariable region of the 16S rRNA gene and analyzed food web dynamics with stable carbon (δ13C) and nitrogen (δ15N) isotopes. We found that insects and spiders were enriched in δ15N at one site downstream of wastewater outfalls, indicating exposure to effluents and transfer of nutrients to terrestrial ecosystems. Microbiomes of most larval and adult insects were altered downstream of wastewater outfalls and had lower relative abundances of endosymbiont bacteria, shifts in bacterial diversities, increases in abundances of effluent-associated bacteria, and downregulation of some biosynthesis pathways than those collected at upstream sites. However, spider microbiomes had little evidence of dysbiosis, and were distinct from those of adult insects, despite a close association in their isotopic signatures. Overall, this study provides evidence of biological impacts from MWWEs to exposed insects and suggests that changes in microbial communities of invertebrates may be used as an effective indicator of effluent exposure as part of monitoring frameworks. / Thesis / Master of Science (MSc) / Municipal wastewater treatment facilities release contaminants into aquatic ecosystems that may affect the health of exposed organisms, including their microbiome, which contains bacteria essential for host digestion and immune function. Alterations in microbiomes of contaminant-exposed aquatic insects are poorly characterized and such effects may be transferred to terrestrial ecosystems through emergent insects. This study evaluated microbiomes and food web dynamics of freshwater insects and riparian spiders upstream and downstream of wastewater treatment facilities in the Bow River, AB. Results indicate that microbiomes of some downstream aquatic insects had lower relative abundances of endosymbiont bacteria, shifts in bacterial diversities, and increases in abundances of effluent-associated bacteria than those collected at upstream sites, but no such changes were observed in the spider predators. This study improves our understanding of how freshwater insect microbiomes are altered by municipal wastewater effluents and suggests that directly effluent-exposed organisms are more at risk of dysbiosis.

Yes / Reverse Osmosis (RO) is widely used for separating organic and inorganic pollutants in wastewater. In this research, the one-dimensional steady state model of a spiral wound RO for the removal of phenol from wastewater, was simulated using gPROMS software to identify optimal design and operating parameters. The design parameters included the membrane length, width and feed spacer channel and operating conditions included temperature and pressure of the RO process. The optimal design parameters were able to maximise the removal of phenol from wastewater. The simulation results showed that the removal of phenol from wastewater was significantly influenced by the combination of membrane width, operating pressure, and feed temperature. The four main parameters (permeate concentration, solute flow, solute rejection, and water flux) that govern the performance of a reverse osmosis membrane were found to be influenced by the design and operating conditions.

An alternative on-site wastewater treatment and disposal system (OSWTDS) consisting of a soil infiltrator with low pressure distribution was evaluated in a soil that was unsuitable for a conventional OSWTDS under current Commonwealth of Virginia Sewage Handling and Disposal Regulations, due to a shallow seasonally perched water table and low hydraulic conductivity. The absorption field consisted of two subsystems numbered as 1 and 2 with effluent design loading rates of 5.1 and 10.2 Lpd/m2, respectively (actual loading rates of 2.4 and 4.9 Lpd/m2, respectively). Soil matric potentials compared seasonally for each subsystem and indicated that both provided similar hydraulic performance. Background water quality was generally improved by subsurface movement through the absorption fields. A bacterial tracer was found in shallow (45.7 cm) and deep (213.4 cm) sampling wells within 24 h in the two subsystems (but in low numbers) over both summer and winter sampling periods. A viral tracer was detected within 48 h in both shallow and deep wells, but only in subsystem 2 in the winter. In evaluating denitrification potential, the addition of glucose to soil core samples did increase quantitatively, although not significantly, nitrous oxide production in each subsystem, at each depth, during each season. Overall, the performance of both subsystems was very similar. The soil infiltrator functioned very well, as designed for the site and soil limitations. It appears to be a potential alternative OSWTDS for use in problem soils. / Master of Science

A two-stage (carbon oxidation stage one, ammonia oxidation stage two) biological aerated filter was operated for 10 months on-site at a domestic wastewater treatment plant. Over the study, the system was operated at different hydraulic loading rates that resulted in a range of applied organic and ammonia mass loadings. Performance was monitored regularly for water quality parameters in the effluent and along the length of the reactors. It was found that nitrification performance was significantly influenced by organic loading rates greater than 1.2 kg cBOD5/m³-d. Additional experiments were conducted in which a constant mass of ammonia was applied (Phase 1: 1.40 ± 0.08 kg NH₃-N/m³-d; Phase 2: 1.31 ± 0.02 kg NH₃-N/m³-d) to the N column, the second stage of the system, over a range of hydraulic loading rates (5.1 -15.8 m/h). Phases of testing were defined by the background hydraulic loading rate applied to the system (Phase 1: 8.3 m/h; Phase 2: 7.1 m/h) at which the reactors were allowed to reach a steady effluent quality for at least one week prior to testing. Organic loading was minimized and kept relatively constant throughout the hydraulic loading rate experiments (0.65 ± 0.2 kg cBOD5/m³-d) in order to obtain an evaluation of nitrification capacity with minimal competition from heterotrophic bacteria. Results indicated that nitrification performance improved by 17% as the applied velocity increased over the indicated range. A steady-state biofilm model capable of predicting substrate flux was applied to the data in an attempt to explain the improvement in performance with hydraulic loading rate from a fundamental standpoint. Mass transfer coefficients, KL, were derived from the model for conditions in which the experimentally observed flux correlated with the model predictions. Derived KL values were lower than estimations offered by correlation equations but increased with velocity at a similar rate. The model failed to account for changes that may have occurred in biofilm kinetics and structure throughout the length of the reactor. / Master of Science

Dewatered sludges and biosolids generated from wastewater treatment facilities are known to emit malodorous odors causing public inconvenience. The odors typically comprise of reduced organo sulfur based compounds and nitrogen containing compounds. Lime stabilization is a technique which is commonly used in the wastewater industry to produce biosolids having reduced odors that can be safely land disposed. In this research, odors produced from dewatered sludges and lime stabilized biosolids were investigated. Lime dosing and incorporation in sludge play an important role in generation of reduced sulfur and trimethylamine (TMA) odor compounds. Results revealed that poor lime dosing can lead to an increase in odors due to biological generation of volatile sulfur compounds (VSCs) during storage. In this study, a belt filter press gave a higher production of sulfur and TMA odors compared to a vacuum filter for the same sludge, which is attributed to the shear imparted to sludge during the dewatering process. Preliming studies suggested incomplete mixing of lime with sludge led to biological activity. The achievement of the correct pH and its maintenance during storage is considered critical for effective odor management from lime stabilized biosolids. A positive linear relation was obtained between sulfur based odor production and labile protein content in sludge. Furthermore, as the Al/Fe ratio increased, the labile proteins was observed to decrease. Trivalent metals are found to play an important role in binding of labile proteins thus effecting odor potential contained in sludge/biosolids. This was found true for most sludge irrespective of their liming status and independent of upstream process conditions. Further work in this area is needed to be able to provide a better understanding of odor production to aid in development of odor control techniques. Trimethylamine odors, having a characteristic fishy odor, are commonly found in lime stabilized biosolids. Cationic polymers used as dewatering aids are the primary precursors for TMA production. Proteins present in sludge are also associated with odor forming compounds but they produce much lower levels than polymers. These two components under the action of shear present in dewatering devices such as centrifuge are more likely to cause an increase in odor production from lime stabilized biosolids. It was also determined that abiotic polymer degradation to produce TMA either does not occur, or the rate is so slow that TMA production in this way is insignificant for actual field situations. / Master of Science

Aquaculture, the cultured production of fish, is growing at a rapid pace worldwide. The industry is generating approximately 140,000 cubic meter wastewater per year. For this industry to flourish, viable methods for treating the resulting waste stream must be identified. The various methods were tried by many researchers like sand filtration method, recirculating aquaculture system, intermittent filtration methods. The most of the industries use sand filtration methods for treating aquaculture wastewater and the problems associated: the reduction in hydraulic conductivity, accumulation of solid due to which anaerobic conditions developed. This study investigated possible treatment technologies for wastewater and sludge produced from Blue Ridge Aquaculture (BRA), an indoor, recirculating aquaculture facility where tilapias (Oreochromis) are raised. Research focused on the use of vermicomposting in conjunction with sand bed filtration to filter aquaculture waste and treat the resulting solids. Two experiments were conducted: a feedstock acceptability test and a filter bed test. The feedstock acceptability test evaluated the suitability of the fish sludge (mixed with cardboard) as a feedstock for the worms involved in the vermicomposting process. The results showed that as the percentage of fish sludge in the feed increased from 0 to 50%, there was a corresponding increase in the growth rate of E.fetida biomass. The filter bed test appraised the feasibility and effectiveness of incorporating vermicomposting in sand filter beds to directly treat aquaculture wastewater. Popular in early wastewater treatment systems, sand filtration has seen a resurgence in recent years. To test the potential for even more effective filtration, sixteen sand filter beds were established--twelve that included worms and four that did not. Wastewater (1.5 % total solids) from BRA was applied to the sand beds at loading rates of 400 to 1000 grams of volatile solids/m2/week. Filter beds containing worms exhibited no ponding over the 70-day experimental period. However, all units without worms failed (exhibited ponding) by the 24th day of operation. Removal efficiencies obtained from the filter bed study for total solids (TS), volatile solids (VS), total suspended solids (TSS), chemical oxygen demand (COD), total phosphorus (TP), sulfate, chlorides, and ammonia-N were greater in filter beds with worms than beds without worms. The worms were crucial to maintaining porosity in the filter beds, hence keeping the filters functioning over time. Worm filter beds removed approximately 100% of the TS, VS, TSS and Ammonia-N, 90% of the TP, 50% of the chlorides, 80% of the sulfate and 70% of the COD. Maximum hydraulic conductivity of 35 cm/day was achieved at the maximum application rate. All the worm filter beds therefore had greater hydraulic conductivity than filter beds without worms. The potential impact is to treat the wastewater effectively, to increase the flow of water, and may be to maintain the aerobic conditions on the worm filterbeds. / Master of Science

京都大学 / 新制・課程博士 / 博士(工学) / 甲第25267号 / 工博第5226号 / 京都大学大学院工学研究科都市環境工学専攻 / (主査)教授 高岡 昌輝, 教授 藤原 拓, 准教授 大下 和徹, 教授 西村 文武 / 学位規則第4条第1項該当 / Doctor of Philosophy (Engineering) / Kyoto University / DFAM

Many countries, including South Africa, are facing the reality of insufficient water supplies to meet their present and future water demands due to decreasing freshwater availability and increasing demand. Increased populations and climate changes further exacerbate the problem. South Africa is considered the thirtieth driest country in the world with limited supplies of water which are unevenly distributed. Thus there is a need for alternative water sources to augment the freshwater supply. Wastewater reuse has been identified worldwide as a viable option to augment water supplies. While technologies are available to ensure proper treatment of wastewater to even potable standards, many countries have experienced public resistance to wastewater reuse due to negative perceptions of consumers. For wastewater reuse initiatives to be successful public acceptance is imperative. The aim of this study was to assess perceptions of consumers in the Vaal Triangle on wastewater reuse. This is the first study conducted in the Vaal Triangle on wastewater reuse hence this information can be valuable in future when wastewater reuse projects are implemented A questionnaire to measure perceptions of consumers on wastewater reuse was developed based on previous studies and distributed to people residing in the Vaal Triangle area. A response rate of 74% (515 completed questionnaires) was obtained from 700 distributed questionnaires. The results showed that socio-demographic factors such as age, race, qualification and level of employment affect the perceptions of consumers on wastewater reuse. Additionally, knowledge of wastewater reuse and water scarcity had a positive effect on consumer‟s perceptions. Some of the major reasons why consumers are not receptive to wastewater reuse are health concerns, lack of trust in the implementing agencies, poor management of the plants and safety of chemicals used to treat the water. Lack of knowledge on wastewater reuse was raised as a major concern in the study. All these concerns need to be addressed to ensure success of wastewater reuse projects within the area. Limitations within the study were identified and recommendations for future research were made. / MBA, North-West University, Potchefstroom Campus, 2015

Many countries, including South Africa, are facing the reality of insufficient water supplies to meet their present and future water demands due to decreasing freshwater availability and increasing demand. Increased populations and climate changes further exacerbate the problem. South Africa is considered the thirtieth driest country in the world with limited supplies of water which are unevenly distributed. Thus there is a need for alternative water sources to augment the freshwater supply. Wastewater reuse has been identified worldwide as a viable option to augment water supplies. While technologies are available to ensure proper treatment of wastewater to even potable standards, many countries have experienced public resistance to wastewater reuse due to negative perceptions of consumers. For wastewater reuse initiatives to be successful public acceptance is imperative. The aim of this study was to assess perceptions of consumers in the Vaal Triangle on wastewater reuse. This is the first study conducted in the Vaal Triangle on wastewater reuse hence this information can be valuable in future when wastewater reuse projects are implemented A questionnaire to measure perceptions of consumers on wastewater reuse was developed based on previous studies and distributed to people residing in the Vaal Triangle area. A response rate of 74% (515 completed questionnaires) was obtained from 700 distributed questionnaires. The results showed that socio-demographic factors such as age, race, qualification and level of employment affect the perceptions of consumers on wastewater reuse. Additionally, knowledge of wastewater reuse and water scarcity had a positive effect on consumer‟s perceptions. Some of the major reasons why consumers are not receptive to wastewater reuse are health concerns, lack of trust in the implementing agencies, poor management of the plants and safety of chemicals used to treat the water. Lack of knowledge on wastewater reuse was raised as a major concern in the study. All these concerns need to be addressed to ensure success of wastewater reuse projects within the area. Limitations within the study were identified and recommendations for future research were made. / MBA, North-West University, Potchefstroom Campus, 2015

The most common type of nitrogen removal in wastewater treatment plant is to use abiological treatment. When biological treatment is used, the two most common processes fornitrogen removal is pre and post denitrification. In the biological treatment A (BA) atKungsängsverket the nitrogen removal process used is intermittent aeration. Whenintermittent aeration is used, the wastewater is aerated at specific time intervals. This allowsboth nitrification and denitrification to occur in the same water volumes. It is of great interestto find a control strategy for controlling the air supply which is both cost effective and leadsto low emissions of nitrogen. The aim of this project was to improve the intermittent aeration at Kungsängsverket, Uppsala.The project was divided into two parts. The first part consisted of experiments carried out infull scale on the plant and the second part was a simulation study. In the experimental partdifferent time intervals for aeration were evaluated. The aim of the simulation part was toevaluate different control parameters and to find which parameters that should be used for anoptimal control strategy. The results showed that the best combination of aerated time and un-aerated time is 50minutes aerated and 50 minutes unaerated. It is recommended that the wastewater should beadded in the beginning and after half of the plant. The simulation results showed that thecontrol strategy can be improved by using a PI-regulator. The experiments and thesimulations both showed clear signs of variations in the inflow during the day. Because of thisit is recommended to establish a control strategy which easily can change the aeration timedepending on low and high flows. / Biologisk rening av avloppsvatten är den vanligaste typen av kväverening vidavloppsreningsverk. Bioblock A, Kungsängsverket Uppsala, har efter en renovering fått dettidigare driftsättet, kaskadkväverening, utbytt mot intermittent luftning. Intermittent luftninginnebär att nitrifikation och denitrifikation sker i samma zoner. Luftningen startas och stängsav med jämna tidsintervall vilket skapar en miljö för både nitrifikation och denitrifikation. Detär av stort intresse att finna en fungerande styrstrategi för luftningen som både ärkostnadseffektiv och som ger låga halter av kväve i utgående vatten. Syftet med examensarbetet var att effektivisera den intermittenta luftningen vid bioblock Avid Kungsängsverket, Uppsala. Tillvägagångsättet var att genom en utvärdering av detnuvarande driftsättet se vad som kunde förbättras. Det ställdes därefter upp ett antal försöksom var inriktade på att finna bättre driftparametrar. Dessa experiment genomfördes ifullskala. Därefter genomfördes en simuleringsstudie där dagens relästyrning jämfördes medtre regleralternativ. Det första alternativet var att reglera luftningen med hjälp av en PIregulator.De andra två alternativen var att styra luftningsperioderna efter utgåendeammonium-respektive nitrathalt. Fullskaleförsöken indikerade att luftning påslagen i 50 minuter följt av avslagen i 50 minutergav den högsta kvävereduktionen av de undersökta alternativen. Bioblock A är uppdelad ifem olika linjer vilka i sin tur är uppdelade i zoner. Utifrån försöken kan det rekommenderasatt avloppsvattnet tillsätts till första zonen och efter hälften av linjen, istället för som vid dennuvarande driften då vattnet tillsätts efter en fjärdedel och efter hälften av linjen. Simuleringsstudien visade att om dagens relästyrning byttes ut mot en PI-regulator skullesyretopparna minskas och syrehalten stabiliseras vid det förinställda börvärdet. Resultatenfrån både simuleringsstudien och fullskaleförsöken visar att en tidsstyrning avluftningsperioderna är av intresse för att spara energi och få en bättre kväverening.