M.Sc. (Aquatic Health) / Wetlands play an extremely important role in the hydrological cycle as well as biodiversity. Until recently, however, wetlands in South Africa have been neglected both in terms of research and monitoring. The lack of knowledge is even greater for the inward draining wetlands, which is better known as pans. Pans are isolated aquatic systems, which are quite shallow and do not have an outlet. Pans receive water from precipitation and surface runoff, and lose water mainly through evaporation. Some pans can be ephemeral (dry up often) and other perennial (dry up only during drought). The little ecological knowledge available is mainly focused on these ephemeral pans. In this present study conducted in the Western Free-State province, study was only focused on the perennial pans in this area. Due to the fact that this province are one of the biggest areas for gold mining in the country, the pans suffer under the impacts, and this results in the alteration of community structures in and around the pan, simplifying food chains which can lead to the collapse of an ecosystem. The aim of the study was to assess the water quality, bioaccumulation of toxicants (metals and pesticides) in sediment and aquatic invertebrate community of perennial pans in the Western Free State Province, mainly because of the lack of literature on pans in these areas. Seventeen pans were selected for the study of which eight were classified as saline eutrophic and nine as alkaline dystrophic. Various physico-chemical and sediment characteristics were assessed in addition to the characterization of the aquatic invertebrate communities. It became clear that there is great difference in the physical-chemical characteristics of the selected pans. The difference in the trophic state was the most clear, with differences in water colour, nutrient concentration and vegetation growth, classifying pans as either saline eutrophic or alkaline dystrophic. These trophic states influence the distribution and richness of aquatic invertebrates in certain pans. Mining activity, urbanization, agricultural activities and sewage runoff into the pan, all affects the pans negatively, there is sighs of water quality degradation as well as changes in the community structure of invertebrates when compared to the reference pans.

Submitted in partial fulfillment for the Degree of Master of Applied Sciences in Biotechnology, Durban University of Technology, Durban, South Africa, 2017. / The study aims to assess and compare the concentration of microbial contaminants, their sources and distribution in surface water and sediment, and to determine the impact of seasonal variations and corresponding risks of faecal contamination using conventional and molecular methods. Historical data analysis was conducted using E. coli values from the eThekwini Water and Sanitation (EWS) department for 66 months (2009-2014). E. coli and Enterococci were analysed in surface water and sediment samples using the mFC/ spread plate and Colilert-18 (IDEXX) methods. The impact of seasonal variations was assessed using E. coli and Enterococci data collected during rainfall and no rainfall events, using an auto-sampler and sediment trap in parallel. Conventional standard membrane filtration methods using mFC agar, Slanetz & Bartley/ Bile Esculin and Brilliance E. coli selective agar were compared to the enzymatic Colilert-18 and Enterolert (IDEXX) test methods along the Isipingo and Palmiet Rivers. In addition, comparison of the analytical performance of droplet digital PCR (ddPCR) and qPCR for the detection of Salmonella targeting ttr gene in river sediment samples collected from the four sites of the Palmiet River in Durban, South Africa was done. In order to assess the public health risk associated with exposure of men, women and children to microbial pathogens in polluted surface water during recreational activities, the QMRA tool was employed in relation to the risk exposure to pathogenic E. coli, Campylobacter, Salmonella and Shigella. Also, the risk associated with crop irrigation (on farmers) as well as the consumption of crops irrigated with surface water from the Isipingo river was determined. Analysis of the historical data gave a baseline of the two rivers of interest, thus helps understand the current situation of the rivers enabling researchers to pick up potential gaps. In this study after the analysis of the historical data it was evident that at the Palmiet river, microbial analysis must be conducted around the QRI settlements which is a major pollution source. Also, from this study it was found that sampling points situated close to wastewater treatment plants, pump stations or informal settlements were of major concern, thus were considered for the study. It was found that sediment exhibited higher microbial concentrations than surface water, which was observed in both rivers. Also, rainfall had a significant impact on microbial variability. Higher microbial concentrations (indicator organisms) were observed in surface water after a heavy rainfall as appose to when there was no rainfall. This was due to contamination that is washed off into the river and sediment resuspension. Methodology comparison revealed that Colilert-18 and Brilliance E. coli were more selective compared to mFC agar. Brilliance E. coli /Coliform agar was comparable with Colilert-18 IDEXX, which was also observed with Slanetz & Bartley and Enterolert IDEXX. However, when mFC agar was compared with Colilert-18 IDEXX, significant difference was observed. In comparison of two Molecular methods, ddPCR were found to be fully amenable for the quantification of Salmonella and offer robust, accurate, high-throughput, affordable and more sensitive quantitation than qPCR in complex environmental samples like sediments. Quantitative Microbial Risk Assessment (QMRA) relating to recreational and occupational exposure showed that children were at the highest risk of getting infected. Also, it was observed that the probability of infection upon exposure to surface water from the Isipingo and Palmiet rivers was significantly high, hence exceeded the WHO guidelines values. Risk assessment on crops revealed that pathogenic bacteria may pose a risk to the consumer, however, a 9-log reduction may be achieved according to the WHO multi-barrier approach which involves proper washing and proper cooking of the crop before ingestion. Overall the sampling points that had the highest pollution level and constantly exceeded the WHO and DWAF guidelines at the Isipingo river were the points situated and named “Next to the WWTP”, and “Downstream of QRI” at the Palmiet River. / M

Ph.D. (Zoology) / Please refer to full text to view abstract

M.Sc. / The water quality monitoring project undertaken on the Blyde River in Pilgrim's Rest and surrounding area, provided information on various parameters. These included the macro variables (pH, temperature, conductivity, dissolved oxygen concentration, oxygen saturation percentage and turbidity), micro variables (ionic and metal concentrations) and fauna (fish and invertebrates). Field and experimental data were integrated to form a realistic and representative picture of the general state of health of the Blyde River. The macro variables (pH, temperature, conductivity, dissolved oxygen concentration, oxygen saturation percentage and turbidity) were all within the guideline limits as provided by the Department of Water Affairs and Forestry. The ionic concentrations (calcium, magnesium, sodium, chloride and nitrate) were acceptable and conformed to guideline values. The aquatic macro-invertebrate and habitat quality survey indicated that most of the sampling sites could be described as good. Sampling site 2 (next to reduction works) was classified as poor. This is the result of mining activity at this specific site situated at the rock dump at Peach Tree Creek. The extensive disturbance of the habitat is the result of 130 years of mining activities. The metal concentrations in the Blyde River exceeded the guideline limits, except for manganese. Aluminium toxicity is pH dependent, and increases with a decrease in the pH. The alkaline pH in the Blyde River acts as a buffer against the aluminium toxicity. The lead concentration in the Blyde River exceeded the Department of Water Affairs and Forestry guideline limit. Lead is hazardous to most forms of life. Zinc is toxic to fish and aquatic organisms at relatively low concentrations. Although nickel is a natural element of the earth and water, mining activities cause nickel emissions and an increase in the nickel concentrations in water systems. Copper is regarded as a highly toxic metal if present in high concentrations. Iron is not easily absorbed by aquatic organisms and because of its limited toxicity and bioavailability, described as a noncritical element. Chromium is biologically inert. Despite of a lack of information on the concentrations of metals in the Blyde River before the onset of mining activities at Pilgrim's Rest, it is clear that the extensive disturbance of the habitat at Peach Tree Creek and Vaalhoek, and to a lesser extent at Delvers' Creek exists. Present and past mining activities in the Pilgrim's Rest area resulted in the deterioration of the water quality in the Blyde River. Rehabilitation of the area around the rock dump at Peach Tree Creek will have to be done to prevent seepage from the rock dump and disturbed area to the river. Measures, such as the construction of paddocks and berm walls to collect seepage before it reaches the river, will have to be implemented. The water will then have to be treated before releasing it into the river. The acute, sub-lethal toxicity tests with nickel and lead were conducted based on the concentrations as derived from water samples taken from the Blyde River, during the monitoring phase of the project. The results of the blood physiological experimentation indicated that normal physiological functioning were disturbed when fish were exposed to sub-lethal concentrations of lead and nickel. Results indicated that stimulation of the immune system, osmotic stress and hypoxic conditions arise after lead and nickel exposure. Hypoxia arises because of a deficiency in the amount of oxygen reaching bodily tissues. Osmotic stress is a result of affected gill and kidney functioning. The metabolic variables indicated that the metabolism of trout was affected, because of stress associated with metal exposure. The nervous system functioning was also, affected, as derived from the enzymatic variables. Taking these factors into consideration, the normal functioning and survival of rainbow trout (Oncorhynchus mykiss) is hindered. The acute, lethal toxicity tests of nickel on rainbow trout (Oncorhynchus mykiss) and chubby head barb (Barbus anoplus) were performed using nickel concentrations as determined in the Blyde River. The 100% mortalities recorded, when exposing the fish to these concentrations, indicated that the Blyde River provides a buffer action against the toxic effect of nickel on the aquatic organisms. Should this buffer action be disturbed, for instance a sudden drop in water pH, the toxicity of nickel will increase and have detrimental effects on the aquatic organisms. Further deterioration of the water quality in the Blyde River should be avoided at all cost. Large scale yellow fish (Barbus marequensis) were only found at sites below sampling site no 8, which is alarming and a further confirmation of habitat degradation. To prevent further degradation of the water quality in the Blyde River annual biomonitoring in conjunction with experimental studies are suggested to obtain information, which enables effective managerial decision making. Measurements should be taken to reduce the impacts of mining and agricultural activities, if future studies indicate further water quality degradation. Objectives regarding the health of the aquatic environment should be set and actions taken to prevent the impactors from doing further harm. The Pilgrim's Rest area can be developed for Eco-tourism, and therefore the aim should be to create awareness of the river and its surroundings and involve people in the conservation thereof.

This thesis is based on a case study of the degraded Boksburg Lake social-ecological system and an environmental education initiative that aimed to support its transformation. This initiative aimed to involve local people in reclaiming the lake’s social and ecological value, through a process of collectively reimagining possibilities, shaping identities, gaining knowledge and developing local human agency. The focus was on social learning processes in schools and churches to explore opportunities for co-engaged reflexivity that might produce transformation. Schools and Christian churches, two institutions that reflect modern, western socialecological worldviews also have the potential to bring about change. Critical Realism was chosen as my philosophical framework as it provided the tools to explore deeper mechanisms beyond empirical reality, both influencing the degrading trajectory as well as providing possibilities for transformation. It also legitimised case study research as a means to understand more generalised processes characterising modern social-ecological systems. The choice of Critical Realism informed the scope of my primary research question: What generative mechanisms constrain and enable the development of social-ecological resilience for well-being, in the modern social-ecological system of Boksburg Lake? The following three goals were formulated to address this primary question. Goal 1: Based on a multitheoretical perspective of social-ecological literature, develop conceptual tools that have explanatory power to probe generative mechanisms operating in the Boksburg Lake social-ecological system. Goal 2: Identify generative mechanisms driving the current degradation of the Boksburg Lake social-ecological system. Goal 3: Identify learning mechanisms that support transformation for greater social-ecological resilience of the Boksburg Lake social-ecological system. By addressing the primary question and research goals I aimed to gain insights into modern global socialecological systems, the mechanisms that drive high social-ecological risk and the requirements for and possibilities of global systemic change. Drawing on a broad reading of social-ecological literature from different vantage points, tools with explanatory power were developed to probe for generative mechanisms in the Boksburg Lake social-ecological system (goal 1). The human capacity for symbolic representation is identified as an emergent property of coevolving human-ecological systems. These symbolic representations become expressed in culture and worldviews, and influence patterns of identifying, types of knowledge and forms of agency. The nature of these will determine the degree that cultural systems are embedded within ecological reality and the extent of cultural-ecological coupling. A cultural system closely coupled with ecological realities is likely to value ecological systems and manage them for their health, while less coupled cultural-ecological systems are likely to lead to the opposite. Because of their integrated nature, the extent of ecological health and value will affect the decline or sustainability of cultural-ecological systems. There are numerous examples of the learning that can take place when cultural-ecological systems are facing decline. This learning can enhance or reduce biophyllic instincts that become encoded in patterns of identifying, types of knowledge and forms of agency. This in turn affects the strength of cultural-ecological coupling and the extent that human societies co-evolve with ecological systems. / Normalising ideologies is a concept coined in the thesis to refer to symbolic representations of reality that have become integral to a social fabric and determine meaning, while maintaining the domination of the powerful. These ideologies determine patterns of identifying, knowledge and agency and are recognised as having a fundamental influence on the resilience of social-ecological systems. Four normalising ideologies are identified that promote apparent human progress at the expense of ecological integrity and social equality and thus alienation with each other and the ecological world. These are human-ecological dualism, anthropocentrism, nature is mechanised and nature is to be controlled. There are also a number of ideologies promoting connectedness with the ecological world that, if they became normalised, would support greater social-ecological resilience for well-being. Generative mechanisms driving the current degradation of the Boksburg Lake socialecological system were identified (goal 2). Drawing on critical methodology, the main method adopted was document analysis of the Boksburg Advertiser archives, Boksburg’s local newspaper. Four generative mechanisms are recognised as most influential. Two of these have been named hegemonic symbolic systems. The primary symbolic system consists of the four normalising ideologies, mentioned above, that promote human progress at the expense of ecological health. The secondary, more explicit symbolic system, built on this, consists of the following normative ideologies: economic growth is imperative, unrestrained development is promoted, competition is the necessary means and consumerism is the good life. These two symbolic systems have had causal influence on the systematic erosion of ecological processes and biological diversity that has occurred in Boksburg, with the consequent undermining of social-ecological resilience for well-being. The third mechanism that constrains resilience is the power dynamics that have shaped Boksburg’s economic history and social-ecological system. This has resulted in a society built on inequality and injustice with all its associated social and environmental ills, expressed as externalities. The fourth mechanism resides in Boksburg’s political and municipal dynamics. These structures are not designed to tackle complex social-ecological problems and they hold considerable agential power, yet seem dysfunctional at present. Learning mechanisms that support transformation for greater social-ecological resilience of the Boksburg Lake social-ecological system were identified (goal 3). By adopting the role of a reflexive practitioner, supported by action research, case study and interpretivist methodologies, data on the empirical manifestations of the environmental educational initiative were collected. Methods included semistructured interviews, focus groups, document analysis and participant observation. Findings indicate that schools and churches are important institutions that can positively influence patterns of identifying, knowledge about and agency for Boksburg Lake and can thus play a role in transforming hegemonic normalising ideologies. Important learning mechanisms identified included: Learning reflexively together within communities of practice that provide opportunities for active rather than passive learning; involving the youth as they are a group of people with notable enthusiasm, vision, energy and motivation; learning through information acquisition, investigation, action and deliberation; learning about abstract concepts and theoretical knowledge but embedding this in local realities; and learning that provides reference markers for how things can be different.

Environmental Education is a foundation in strengthening learners and community awareness in all environmental issues, including water pollution-related diseases. This study aimed at investigating the level of environmental education awareness on water pollution-related diseases by learners who live in the informal settlement. And lastly, if possible and feasible, to propose solutions to the perceived water pollution-related diseases found in this informal settlement adjacent to the Jukskei river in Alexandra Township, Johannesburg. The research focused on the introduction of the dissertation in Chapter 1 Theoretical foundations of the research in Chapter 2 Research methodology in Chapter 3 Data analysis, interpretation and discussion in Chapter 4 Conclusions and recommendations in Chapter 5 The study provided various definitions of environment, Environmental Education, The goal, Aims & Objective, Classification & Outcomes, Environmental literacy; water pollution-related diseases, the integration of Environmental education in the RNCS & water pollution-related diseases awareness; Education for Sustainable Development; and the relationship between the development of informal settlement and water pollution related diseases. The outcomes of the investigation indicated that the level of environmental education awareness on water pollution –related diseases by learners at the informal settlement is less at primary level and partially increases at the high schools. In order to increase learners’ awareness on water pollution-related diseases across the curricula there should be an adequate integration of Environmental Education in the RNCS. / Educational Studies / M.Ed. (Environmental Education)

Environmental Education is a foundation in strengthening learners and community awareness in all environmental issues, including water pollution-related diseases. This study aimed at investigating the level of environmental education awareness on water pollution-related diseases by learners who live in the informal settlement. And lastly, if possible and feasible, to propose solutions to the perceived water pollution-related diseases found in this informal settlement adjacent to the Jukskei river in Alexandra Township, Johannesburg. The research focused on the introduction of the dissertation in Chapter 1 Theoretical foundations of the research in Chapter 2 Research methodology in Chapter 3 Data analysis, interpretation and discussion in Chapter 4 Conclusions and recommendations in Chapter 5 The study provided various definitions of environment, Environmental Education, The goal, Aims & Objective, Classification & Outcomes, Environmental literacy; water pollution-related diseases, the integration of Environmental education in the RNCS & water pollution-related diseases awareness; Education for Sustainable Development; and the relationship between the development of informal settlement and water pollution related diseases. The outcomes of the investigation indicated that the level of environmental education awareness on water pollution –related diseases by learners at the informal settlement is less at primary level and partially increases at the high schools. In order to increase learners’ awareness on water pollution-related diseases across the curricula there should be an adequate integration of Environmental Education in the RNCS. / Educational Studies / M.Ed. (Environmental Education)

Thesis (M. Tech. (Agriculture)) -- Central University of Technology, Free state, 2011 / Quality assessment of agricultural water used for fertigation in the Boland district. The study was undertaken to determine the influence of seasonal changes on the water quality (chemical and microbial analyses) of five different rivers in the Boland. The data was collected in the five most important water sources that are used for irrigation purposes in the district, i.e. Berg river, Eerste river, Klapmuts River, Klippies river and Krom river. The samples were collected from all sites once every three weeks for a period of six months. The sampling was carried out during specific periods in summer (December, 2006 to February, 2007) and winter (June to August, 2007). The results of the study showed that Klapmuts river recorded the highest levels of chloride and iron, especially in summer. However, chloride levels were far below the levels set by the Department of Water Affairs and Forestry. Results obtained from this study show the presence of pathogens in some of the rivers assessed which may have resulted from the leaching of these pathogens from nearby agricultural land, livestock watering or informal settlements in the catchment areas. The bigger rivers recorded low levels of micro-elements and this might have been affected by winter rainfall. In the Berg River, many sources of nitrate pollution seem to be present in the catchment area. The levels of iron in all the rivers assessed were far more than the levels set by the Department of Water Affairs and Forestry in all rivers assessed and these might be due to the pH levels and interaction between the rivers and seasons. Iron and manganese levels should be kept low as this may cause production problems by blocking irrigation drippers. The water samples tested for bacterial and fungal density showed Klapmuts and Eerste rivers were positive for Phytophthora cinnamomi during winter. Phytophthora citricola and Phytophthora cactorum were detected in the Klapmuts and Klippies rivers in summer. The Berg-, Klapmuts-, Krom- and Eerste rivers tested positive for species of the genera Pythium and Fusarium. Similar organisms were detected in the Eerste river mainly during summer on the fourth sampling date, while Krom river only tested positive for Pythium during summer. The total bacterial and algal density differed significantly between the seasons and was highest in winter. This might be due to high rain water influx and efflux and/or moist and aerobic conditions and air temperature. There is an increased need for farmers to sterilize feeding water (chlorination) due to high microbial count.

Surface water pollution is prevalent in numerous areas of central Roodepoort mainly due to gold mining activities. The surface water quality for the Bosmontspruit, Russell’s Stream and the New Canada Dam was assessed from October 2010 to March 2011. Physical, chemical and biological characteristics of the water were determined for 8 monitoring points and the results obtained were compared with the In-stream water quality guidelines for the Klip River catchment and the South African Water Quality Guidelines. A trend noticed throughout the sampling period was the non-compliance in the levels of total dissolved solids (TDS) and dissolved oxygen. The results indicated that concentrations of iron, aluminium, nickel, manganese and potassium were above the limit across the Bosmontspruit and Russell’s stream. There was also significant evidence of excessive faecal coliform and ammonium pollution in the Bosmontspruit. During the monitoring period it was noted that water from these streams were utilised for crop irrigation, bathing, livestock and human consumption and may pose a health hazard due to poor water quality. / Environmental Sciences / M.Sc. (Environmental Science)

In South Africa water is recognized as a crucial element in the battle against poverty, the cornerstone of prosperity, and a limiting factor to growth. The National Water Act 36 of 1998 recognizes that basic human and environmental needs should be met and that the use of water in all aspects must be sustainable. The Crocodile River (East) is situated in the north east of the Republic of South Africa and it is recognized as a stressed catchment in South Africa. The main impacts are domestic, industrial, agricultural, mining and afforestation activities. These activities pollute the river by discharging effluent as well as seepage from areas that support mining and intensive agriculture in to the river. The river catchment has been a center of research studies for many scientists either focusing on water quality or biological indicators separately. The aim of study was to determine the present ecological condition and the health of the Crocodile River. The objectives were to determine water quality status, identify possible sources of pollution and assess the spatial and temporal trends in ecological state. Fourteen monitoring sites were selected from the Crocodile River and its tributaries. The macro-invertebrates data were collected using the SASS 5 protocol and fish were collected using an electroshocker- catch and release method during high and low flow conditions of the year 2013. Water quality data was obtained by sampling using a polyethylene bottle from different sites within the Crocodile River and its tributaries from September 2012 until August 2013. The samples were analyzed by Mpumamanzi laboratory in Nelspruit and Waterlab in Pretoria. Additional water quality data was obtained from the Department of Water Affairs. Multivariate statistical methods were used to analyze all the data obtained. The multivariate statistical methods indicated that fish and macro-invertebrates species abundance, richness and evenness increase with the river flow distance downstream. Water temperature was one of the leading environmental variables for the structuring of fish and macro-invertebrates assemblage in the Crocodile River and its tributaries. A group formation of site during high and low flow condition by the Bray Curtis similarity and NMDS ordination indicated that many sites share similar macro-invertebrates or fish species. The one way ANOVA analysis indicated that there was no significance difference between macro-invertebrates richness and abundance during both flow conditions but there was a significance difference in fish richness and abundance between the two flow conditions. The PCA indicated that sodium has the highest physico-chemical impact amongst the physico-chemical parameters in the bi-plot followed by ammonium and nitrate. A correlation of physico-chemical parameters such as chlorine, total dissolved solid, electrical conductivity, sulphate and pH was observed. The levels of total dissolved solids and electrical conductivity were found to increase longitudinally as the river flows downstream. The dominance of single species within the macro-invertebrates and fish communities was an indication of imbalance of the communities within the sites. The presence of the macro-invertebrate family Beatidae and the fish species Chiloglanis pretoriae at sites CR3 and CR4 indicates a good water quality in the upper reaches during low flow condition as these species are sensitive to changes in water quality, while the presence of the macro-invertebrate family Pleidea and fish species Barbus viviparus at sites CR10 and KR1 during low flow condition indicated possible water pollution as these species tolerate changes in water quality and this was linked to the discharge of effluent from industrial, abandoned mines and run-offs from agricultural activities in the downstream reaches of the river. The low flow condition was dominated by sensitive species especially in the upper reaches than during high flow condition. The macro-invertebrates assessment index indicated that during low flow condition the Crocodile River was mostly at ecological class B (largely natural with few modification) above the Kwena dam, but from downstream of the dam the ecological category was in C class (moderately modified), while its tributaries are in ecological category B (largely natural with few modification). The fish response assessment index indicated that the ecological category for fish was mostly at C class (moderately modified) in the Crocodile River. The changes in macro-invertebrates communities and fish in the Crocodile River were believed to be associated with change of water quality and habitat modification due to flow modification. Agricultural activities in the upper reaches and a combination of industrial and mining activities in the middle and lower reaches of the Crocodile River were believed to be the sources of pollution that results in the change of water quality, fish and macro-invertebrates assemblage in the Crocodile River. Investing in the health of the Crocodile River is important for many Mbombela citizens and river dwellers as they rely on the functioning of the river for survival. / Environmental Sciences / M. Sc. (Environmental Science)