The realization of planning, design, construction, operation and maintenance of water supply systems pictures one of the largest infrastructure projects of municipalities / water distribution networks should be designed very meticulously. Genetic algorithm is an optimization method that is based on natural evolution and is used for the optimization of water distribution networks. Genetic algorithm is comprised of operators and the operators affect the performance of the algorithm. Although these operators are related with parameters, not much attention has been given for the determination of these parameters for this specific field of water distribution networks. This study represents a novel methodology, which investigates the parameters of the algorithm for different networks. The developed computer program is applied to three networks. Two of these networks are well known examples from the literature / the third network is a pressure zone of Ankara water distribution network. It is found out that, the parameters of the algorithm are related with the network, the case to be optimized and the developed computer program. The pressure penalty constant value varied depending on the pipe costs and the network characteristics. The mutation rate is found to vary in a range of [0.0075 &ndash / 0.0675] for three networks. Elitism rate is determined as the minimum value for the corresponding population size. Crossover probability is found to vary in a range of [0.5 &ndash / 0.9]. The methodology should be applied to determine the appropriate parameter set of genetic algorithm for each optimization study. Using the method described, fairly well results are obtained.

Quality of municipal water is sustained by addition of disinfectant, generally chlorine, to the water distribution network. Because of health problems, chlorine concentration in the network is limited between maximum and minimum limits. Cancerogenic disinfectant by-products start to occur at high concentrations so it is desired to have minimum amount of chlorine without violating the limit. In addition to the health issues, minimum injection amount is favorable concerning cost. Hence, an optimization model is necessary which covers all of these considerations. However, there are uncertain factors as chlorine is reactive and decays both over time and space. Thus, probabilistic approach is necessary to obtain reliable and realistic results from the model. In this study, a linear programming model is developed for the chance constrained optimization of the water distribution network. The objective is to obtain minimum amount of injection mass subjected to maintaining more uniformly distributed chlorine concentrations within the limits while considering the randomness of chlorine concentration by probability distributions. Network hydraulics and chlorine concentration computations are done by the network simulation software, EPANET.

Diss. Stockholm : Stockholms universitet, 2006.

Access to clean and safe drinking water is a fundamental human requirement. However, in many areas of the world natural water sources have been impacted by a variety of biological and chemical contaminants. The ingestion of these contaminants may cause acute or chronic health problems. To prevent such illnesses, many technologies have been developed to treat, disinfect and supply safe drinking water quality. However, despite these advancements, water supply distribution systems can adversely affect the drinking water quality before it is delivered to consumers. The primary aim of this research was to investigate the effect that water distribution systems may have on household drinking water quality in Christchurch, New Zealand and Addis Ababa, Ethiopia. Water samples were collected from the source water and household taps in both cities. The samples were then tested for various physical, chemical and biological water quality parameters. The data collected was also used to determine if water samples complied with national drinking water quality standards in both countries. Independent samples t-test statistical analyses were also performed to determine if water quality measured in the samples collected from the source and household taps was significantly different. Water quality did not vary considerably between the source and tap water samples collected in Christchurch City. No bacteria were detected in any sample. However, the pH and total iron concentrations measured in source and tap water samples were found to be significantly different. The lower pH values measured in tap water samples suggests that corrosion may be taking place in the distribution system. No water samples transgressed the Drinking Water Standards for New Zealand (DWSNZ) MAVs. Monitoring data collected by the Christchurch City Council (CCC) was also used for comparison. A number of pH, turbidity and total iron concentration measurements collected by the CCC in 2011 were found to exceed the guideline values. This is likely due to structural damage to the source wells and pump-stations that occurred during the 2011 earthquake events. Overall, it was concluded that the distribution system does not adversely affect the quality of Christchurch City’s household drinking water. The water quality measured in samples collected from the source (LTP) and household taps in Addis Ababa was found to vary considerably. The water collected from the source complied with the Ethiopian (WHO) drinking water quality standards. However, tap water samples were often found to have degraded water quality for the physical and chemical parameters tested. This was especially the case after supply interruption and reinstatement events. Bacteria were also often detected in household tap water samples. The results from this study indicate that water supply disruptions may result in degraded water quality. This may be due to a drop in pipeline pressure and the intrusion of contaminants through the leaky and cross-connected pipes in the distribution network. This adversely affects the drinking water quality in Addis Ababa.

The goal of a water distribution system (WDS) is to supply the desired quantity of fresh water to consumers at the appropriate time. In order to properly operate a WDS, system operators need information about the system states, such as tank water level, nodal pressure, and water quality for the system wide locations. Most water utilities now have some level of SCADA (Supervisory Control and Data Acquisition) systems providing nearly real-time monitoring data. However, due to the prohibitive metering costs and lack of applications for the data, only portions of systems are monitored and the use of the SCADA data is limited. This dissertation takes a comprehensive view of real-time demand estimation in water distribution systems. The goal is to develop an optimal monitoring system plan that will collect appropriate field data to determine accurate, precise demand estimates and to understand their impact on model predictions. To achieve that goal, a methodology for real-time demand estimates and associated uncertainties using limited number of field measurements is developed. Further, system wide nodal pressure and chlorine concentration and their uncertainties are predicted using the estimated nodal demands. This dissertation is composed of three journal manuscripts that address these three key steps beginning with uncertainty evaluation, followed by demand estimation and finally optimal metering layout.The uncertainties associated with the state estimates are quantified in terms of confidence limits. To compute the uncertainties in real-time alternative schemes that reduce computational efforts while providing good statistical approximations are evaluated and verified by Monte Carlo simulation (MCS). The first order second moment(FOSM) method provides accurate variance estimates for pressure; however, because of its linearity assumption it has limited predictive ability for chlorine under unsteady conditions. Latin Hypercube sampling (LHS) provides good estimates of prediction uncertainty for chlorine and pressure in steady and unsteady conditions with significantly less effort.For real-time demand estimation, two recursive state estimators; tracking state estimator (TSE) based on weighted least squares (WLS) scheme and Kalman filter (KF), are applied. In addition, in order to find available field data types for demand estimation, comparative studies are performed using pipe flow rate and nodal pressure head as measurements. To reduce the number of unknowns and make the system solvable, nodes with similar user characteristics are grouped and assumed to have same demand pattern. The uncertainties in state variables are quantified in terms of confidence limits using the approximate methods (i.e., FOSM and LHS). Results show that TSE with pipe flow rates as measurements provide reliable demand estimations. Also, the model predictions computed using the estimated demands match well with the synthetically generated true values.Field measurements are critical elements to obtaining quality real-time state estimates. However, the limited number of metering locations has been a significant obstacle for the real-time studies and identifying locations to best gain information is critical. Here, an optimal meter placement (OMP) is formulated as a multi-objective optimization problem and solved using a multi-objective genetic algorithm (MOGA) based on Pareto-optimal solutions. Results show that model accuracy and precision should be pursued at the same time as objectives since both measures have trade-off relationship. GA solutions were improvements over the less robust methods or designers' experienced judgment.

The optimisation of the decision of when to replace water distribution mains is a complex task. There are numerous drivers in the decision making process (informed by financial data, performance data and water quality data) and hundreds of variables and performance indicators that can be considered when trying to reach an optimised decision. Most of the assets under consideration are buried and the internal and external pipe conditions are not easily assessable, forcing the Utility to rely on the available direct and indirect variables from which conclusions on the reliability of the mains are to be inferred. The cost of mains replacement is relatively low but the assessment cost, if carried out can be relatively high. The total value of a metropolitan distribution network typically runs into billions of rands but the impact of an individual pipe failure is generally low. The distribution network is comprised of many different pipe materials and components, of different pressure classes, made by different manufacturers, installed by numerous contractors with different skill levels under differing quality control regimens over many years. To add to this complexity, various parts of the network are operated at different static pressures and varying velocities. Some sections of the network are isolated more often than others and at times there can be large pressure surges that the network is subjected to by either the Utility or Consumer. These pressure surges are known to have a marked detrimental effect on the network. False markers also exist that can give rise to totally incorrect decisions and therefore performance data cannot be accepted at face value and needs to be scrutinised and cleansed to increase its reliability prior to being utilised in decision making process. This important step has been missed by much of the research carried out to date. In the Durban context, a further complication is caused by consumers tampering with the water mains and also not reporting leaks. This has a negative effect on the performance of the water main that can cause it to be flagged for replacement, but its replacement will not result in an increase in performance if the social issues are not resolved first. The aim of this research is to make recommendations on the methodology to be employed to improve network performance and thereby delay the point at which the water mains are to be replaced for as long as possible. These recommended activities will be carried out to remove false markers and improve upon the quality and reliability of the data available on the network performance. A further output is to make recommendations regarding the minimum data that can be reasonably collected and analysed in order to determine an optimised result. The recommendation of which mains should be targeted for replacement should result in the highest benefit for the utility as well as the consumers. By implication, this will result lowest long term capital and operational expenditure and thus the lowest long term tariffs charged to the consumers whilst complying with the water quality criteria and service level targets. / Thesis (M.Sc.Eng.)-University of KwaZulu-Natal, Durban, 2012.

There is currently growing concern about the presence of heterotrophic plate count (HPC) bacteria in drinking water. These HPC may have potential pathogenic features, enabling them to cause disease. It is especially alarming amongst individuals with a weakened immune system. South Africa, the country with the highest incidents of HIV positive individuals in the world, mainly uses these counts to assess the quality of drinking water in terms of the number of micro-organisms present in the water. These micro-organisms may be present in the bulk water or as biofilms adhered to the surfaces of a drinking water distribution system. The current study investigated the pathogenic potential of HPC bacteria occurring as biofilms within a drinking water distribution system and determined the possible presence of these micro-organims within the bulk water. Biofilm samples were taken from five sites within a drinking water distribution system. Fifty six bacterial colonies were selected based on morphotypes and isolated for the screening of potential pathogenic features. Haemolysin production was tested for using sheep-blood agar plates. Of the 56, 31 isolates were ?-haemolytic. Among the 31 ?-haemolytic positive isolates 87.1% were positive for lecithinase, 41.9% for proteinase, 19.4% for chondroitinase, 9.7% for DNase and 6.5% for hyaluronidase. All of the ?-haemolytic isolates were resistant to oxytetracycline 30 ?g, trimethoprim 2.5 ?g and penicillin G10 units, 96.8% were resistant to vancomycin 30 ?g and ampicillin 10 ?g, 93.5% to kanamycin 30 ?g, 74.2% to chloramphenicol 30 ?g, 54.8% to ciprofloxacin 5 ?g, 22.6% to streptomycin 300 ?g and 16.1% to erythromycin 15 ?g. Nineteen isolates producing two or more enzymes were subjected to Gram staining. The nineteen isolates were all Gram-positive. These isolates were then identified using the BD BBL CRYSTALTM Gram-positive (GP) identification (ID) system. Isolates were identified as Bacillus cereus, Bacillus licheniformis, Bacillus subtilis, Bacillus megaterium, Bacillus pumilus and Kocuria rosea. 16S rRNA gene sequencing was performed to confirm these results and to obtain identifications for the bacteria not identified with the BD BBL CRYSTALTM GP ID system. Additionally identified bacteria included Bacillus thuringiensis, Arthrobacter oxydans and Exiguobacterium acetylicum. Morphological properties of the different species were studied with transmission electron microscopy (TEM) to confirm sequencing results. All the isolates displayed rod shaped cells with the exception of Arthrobacter oxydans being spherical in the stationary phase of their life cycle. Bulk water samples were taken at two sites in close proximity with the biofilm sampling sites. The DNA was extracted directly from the water samples and the 16S rRNA gene region was amplified. Denaturing gradient gel electrophoresis (DGGE) was performed to confirm the presence of the isolates from the biofilm samples in the bulk water samples. The presence of Bacillus pumilus and Arthrobacter oxydans could be confirmed with DGGE. This study demonstrated the presence of potentially pathogenic HPC bacteria within biofilms in a drinking water distribution system. It also confirmed the probable presence of two of these biofilm based bacteria in the bulk water. / Thesis (M.Sc. (Microbiology))--North-West University, Potchefstroom Campus, 2010.

The presence of heterotrophic plate count (HPC) bacteria in drinking water distribution systems is usually not considered harmful to the general consumer. However, precautions must be taken regarding the immunocompromised. All water supply authorities in South Africa are lawfully required to provide consumers with high-quality drinking water that complies with South African-and international standards. This study mainly focused on the isolation, identification and characterization of HPC and other bacteria from biofilm-and bulk water samples from two sampling points located within the Potchefstroom drinking water distribution system. Based on five main objectives set out in this study, results indicated that the bulk water at the J.S. van der Merwe building was of ideal quality fit for lifetime consumption. Application of enrichment-and selective media allowed for the isolation of 12 different bacterial morphotypes. These were identified by way of biochemical-and molecular methods as Bacillus cereus, Bacillus subtilis, Brevundimonas spp., Clostridiaceae, Corynebacterium renale, Flavobacteriaceae, Kytococcus sedentarius, Leuconostoc lactic, Lysinibacillus sphaericus, Pseudomonas spp., Staphylococcus aureus and Staphylococcus capitis. The greatest diversity of bacteria was detected early autumn 2008, while the lowest diversity occurred during mid-winter 2007. Bacillus cereus, Kytococcus sedentarius and Staphylococcus capitis displayed potential pathogenic properties on blood agar. Kytococcus sedentarius could be classified as potentially the most pathogenic among the isolates. All isolates displayed multiple-resistant patterns towards tested antibiotics. Corynebacterium renale and Staphylococcus aureus were least resistant bacterial species and Lysinibacillus sphaericus the most resistant. All isolates were susceptible to ciprofloxacin (CIP) and streptomycin (S), but most were resistant to erythromycin (E). Transmission electron microscopy (TEM) allowed for detailed examination of Brevundimonas spp., Pseudomonas spp. and Staphylococcus spp. The capability of Brevundimonas spp. to produce slime and store nutrients within inclusion bodies, suggests the ability of this bacterium to form biofilm and persist in the drinking water for prolonged periods. Despite the inhibitory or toxic effect of copper against bacterial growth, scanning electron microscopy (SEM) revealed the presence of biofilms as well as diatoms on red-copper coupons. Biofilm activity was also observed on reverse-osmosis (RO) filters. Since corrosion was evident on red-copper coupons, it is recommended that prospective studies also look into the significance of microbial induced corrosion (MIC) within the Potchefstroom drinking water distribution system. Other prospects include determining minimum inhibitory concentrations of isolates against antibiotics and the application of culture independent methods such as SSCP and DGGE to investigate biofilm development. The use of diatoms as an index of the drinking water quality is also suggested. / Thesis (M.Sc. (Environmental Science))--North-West University, Potchefstroom Campus, 2010.

There is currently growing concern about the presence of heterotrophic plate count (HPC) bacteria in drinking water. These HPC may have potential pathogenic features, enabling them to cause disease. It is especially alarming amongst individuals with a weakened immune system. South Africa, the country with the highest incidents of HIV positive individuals in the world, mainly uses these counts to assess the quality of drinking water in terms of the number of micro-organisms present in the water. These micro-organisms may be present in the bulk water or as biofilms adhered to the surfaces of a drinking water distribution system. The current study investigated the pathogenic potential of HPC bacteria occurring as biofilms within a drinking water distribution system and determined the possible presence of these micro-organims within the bulk water. Biofilm samples were taken from five sites within a drinking water distribution system. Fifty six bacterial colonies were selected based on morphotypes and isolated for the screening of potential pathogenic features. Haemolysin production was tested for using sheep-blood agar plates. Of the 56, 31 isolates were ?-haemolytic. Among the 31 ?-haemolytic positive isolates 87.1% were positive for lecithinase, 41.9% for proteinase, 19.4% for chondroitinase, 9.7% for DNase and 6.5% for hyaluronidase. All of the ?-haemolytic isolates were resistant to oxytetracycline 30 ?g, trimethoprim 2.5 ?g and penicillin G10 units, 96.8% were resistant to vancomycin 30 ?g and ampicillin 10 ?g, 93.5% to kanamycin 30 ?g, 74.2% to chloramphenicol 30 ?g, 54.8% to ciprofloxacin 5 ?g, 22.6% to streptomycin 300 ?g and 16.1% to erythromycin 15 ?g. Nineteen isolates producing two or more enzymes were subjected to Gram staining. The nineteen isolates were all Gram-positive. These isolates were then identified using the BD BBL CRYSTALTM Gram-positive (GP) identification (ID) system. Isolates were identified as Bacillus cereus, Bacillus licheniformis, Bacillus subtilis, Bacillus megaterium, Bacillus pumilus and Kocuria rosea. 16S rRNA gene sequencing was performed to confirm these results and to obtain identifications for the bacteria not identified with the BD BBL CRYSTALTM GP ID system. Additionally identified bacteria included Bacillus thuringiensis, Arthrobacter oxydans and Exiguobacterium acetylicum. Morphological properties of the different species were studied with transmission electron microscopy (TEM) to confirm sequencing results. All the isolates displayed rod shaped cells with the exception of Arthrobacter oxydans being spherical in the stationary phase of their life cycle. Bulk water samples were taken at two sites in close proximity with the biofilm sampling sites. The DNA was extracted directly from the water samples and the 16S rRNA gene region was amplified. Denaturing gradient gel electrophoresis (DGGE) was performed to confirm the presence of the isolates from the biofilm samples in the bulk water samples. The presence of Bacillus pumilus and Arthrobacter oxydans could be confirmed with DGGE. This study demonstrated the presence of potentially pathogenic HPC bacteria within biofilms in a drinking water distribution system. It also confirmed the probable presence of two of these biofilm based bacteria in the bulk water. / Thesis (M.Sc. (Microbiology))--North-West University, Potchefstroom Campus, 2010.

The presence of heterotrophic plate count (HPC) bacteria in drinking water distribution systems is usually not considered harmful to the general consumer. However, precautions must be taken regarding the immunocompromised. All water supply authorities in South Africa are lawfully required to provide consumers with high-quality drinking water that complies with South African-and international standards. This study mainly focused on the isolation, identification and characterization of HPC and other bacteria from biofilm-and bulk water samples from two sampling points located within the Potchefstroom drinking water distribution system. Based on five main objectives set out in this study, results indicated that the bulk water at the J.S. van der Merwe building was of ideal quality fit for lifetime consumption. Application of enrichment-and selective media allowed for the isolation of 12 different bacterial morphotypes. These were identified by way of biochemical-and molecular methods as Bacillus cereus, Bacillus subtilis, Brevundimonas spp., Clostridiaceae, Corynebacterium renale, Flavobacteriaceae, Kytococcus sedentarius, Leuconostoc lactic, Lysinibacillus sphaericus, Pseudomonas spp., Staphylococcus aureus and Staphylococcus capitis. The greatest diversity of bacteria was detected early autumn 2008, while the lowest diversity occurred during mid-winter 2007. Bacillus cereus, Kytococcus sedentarius and Staphylococcus capitis displayed potential pathogenic properties on blood agar. Kytococcus sedentarius could be classified as potentially the most pathogenic among the isolates. All isolates displayed multiple-resistant patterns towards tested antibiotics. Corynebacterium renale and Staphylococcus aureus were least resistant bacterial species and Lysinibacillus sphaericus the most resistant. All isolates were susceptible to ciprofloxacin (CIP) and streptomycin (S), but most were resistant to erythromycin (E). Transmission electron microscopy (TEM) allowed for detailed examination of Brevundimonas spp., Pseudomonas spp. and Staphylococcus spp. The capability of Brevundimonas spp. to produce slime and store nutrients within inclusion bodies, suggests the ability of this bacterium to form biofilm and persist in the drinking water for prolonged periods. Despite the inhibitory or toxic effect of copper against bacterial growth, scanning electron microscopy (SEM) revealed the presence of biofilms as well as diatoms on red-copper coupons. Biofilm activity was also observed on reverse-osmosis (RO) filters. Since corrosion was evident on red-copper coupons, it is recommended that prospective studies also look into the significance of microbial induced corrosion (MIC) within the Potchefstroom drinking water distribution system. Other prospects include determining minimum inhibitory concentrations of isolates against antibiotics and the application of culture independent methods such as SSCP and DGGE to investigate biofilm development. The use of diatoms as an index of the drinking water quality is also suggested. / Thesis (M.Sc. (Environmental Science))--North-West University, Potchefstroom Campus, 2010.