MESHWR / Department of Hydrology and Water Resources / Siloam, a village in the Northern province of South Africa has groundwater reportedly characterised by concentration of fluoride greater than 1.5 mg/L permissible limit. Regional investigation has been done to determine the possible fluoride sources in the northern province of South Africa, but no localised investigation has been documented. The World Health Organization and South Africa National Standard permissible limit is 1.5 mg/L for a duration of time. Therefore, this study focuses on investigating the sources of fluoride in the groundwater of Siloam village in response to reported high incidences (80%) of dental fluorosis in Siloam village. This study also elaborates more on the hydrochemical and geochemical processes favouring the release of fluoride into the groundwater of Siloam village. Two (2) surface rocks, one (1) surface clay deposit, twelve (12) borehole cuttings and four (4) groundwater samples were used for this study. Temperature, total dissolved solids (TDS), conductivity (EC) and pH of the groundwater were determined using a combined multimeter because these parameters are proxy indicators of geogenic influence on groundwater fluoride concentration. Total fluoride in the groundwater samples and the leachates obtained during a leaching experiment were determined using Ion Chromatograph (IC) and Fluoride Ion Selective Electrode (FISE). X-ray fluorescence spectrometry is the most preferred method for rock analysis by earth scientists because it is faster and does not require ambiguous training on the part of the analyst, therefore, it is mostly the standard methods used in whole rock chemistry. Elemental composition and mineralogy were determined using X-ray Fluorescence (XRF) and X-ray diffraction (XRD), respectively. Clay, greywacke, argillaceous sedmients and basalt were analysed. Results obtained indicated that the groundwater fluoride concentration ranges from 3.92 to 4.95 mg/L, which are far above the WHO permissible limit and South African standard. Insitu temperature reading obtained ranges from 25 to 48°C thus classifying the groundwater to be hot and tepid. The groundwater pH ranges from 8.1 to 9.1 which indicates an alkaline water condition. The groundwater conductivity ranges from 330 to 730 μS/cm while the Total dissolved solids ranges from 130.12 to 423.07 mg/L. Groundwater proxy indicators showed geologic sources to be responsible for the high fluoride concentration in the groundwater of Siloam village. Hydrochemical investigation revealead that the mineralisation of groundwater in Siloam village is as a result of rock water interaction and the process of groundwater enrichment is through base ion exchange and reverse ion exchange. The dominant water type in the village was identified to be Na-Cl water type and the abundance of sodium (Na) is due to the heavy weathering of plagioclase of the parent rocks. Total fluoride content of the rocks and soils of the village ranges from 10 to 2000 mg/L. The leachable fluoride, however, ranged in concentration from 0.27 to 14.88 mg/L under induced temperature v and 0.05 to 10.40 mg/L without temperature. Mineralogical investigation revealed the abundance of smectite clay, which is known for its enrichment and leaching of fluoride into groundwater sources, thus, the research question and assumption about the possible source of fluoride and the assumption that fluoride increases downwards towards the aquifer were answered. Leaching experiments identified the main contributors of fluoride to groundwater in Siloam village as smectite clays and the muscovite present in the sandstone, greywacke and basalt. Time based leaching experiment, geochemical modelling and dating of different hydrogeological unit was recommended / NRF

MESHWR / Department of Hydrology and Water Resources / Lebalelo area of Sekhukhune district is one of many areas in South Africa experiencing portable water scarcity, especially during prolonged dry season. Due to the dominance of low yielding aquifers in South Africa, it is essential to manage groundwater resources in these low yielding aquifers. However, the management of low yielding aquifer is difficult in areas like Labelelo where the hydrogeological characteristics of the aquifers are understudied. This study investigated the hydrogeological characteristics of the aquifers in the area using combined geophysical method and analytical groundwater models. Four newly drilled borehole and five existing boreholes were used for this study. Geophysical survey was carried out using magnetic and electromagnetic methods. The magnetic survey was used to locate the position of magnetic bodies such as dolerite dykes and different lithologies with different magnetic properties. The electromagnetic survey however, was used to determine zones of high permeability associated with the intrusive bodies as well as high permeability zones in fault planes. Step test, constant discharge test and recovery tests were conducted on all the boreholes to stress the borehole. This was used to determine a suitable and sustainable pumping rate of the aquifer. Pumping test data from the pumping period and recovery was evaluated and interpreted using AQTESOLVE. Aquifer transmissivity, storativity, internal and external hydraulic boundaries were determined from the data. The transmissivity in the area ranges from 0.08 to 124.7 m2/day. The aquifer types in the area are double porosity aquifer, radial flow aquifer with single porosity. Inductive Coupled Plasma (ICP-MS) was used to measure heavy metals, trace metals and cations while Ion Chromatography (IC) was used to determine anions in groundwater of the study area. The groundwater in the area is dominated by calcium carbonate as a result of long residence time with dolomite. The hydrochemistry of the water indicates that the chemistry of the groundwater in the area is mainly controlled by rock-water interaction. / NRF

MSc (Microbiology) / Department of Microbiology / See the attached abstract below

MESHWR / See the attached abstract below

MENVSC / Department of Ecology and Resource Management / Some boreholes in South Africa which serve as a source of drinking water for rural communities are reported to have high fluoride concentration, much above the WHO guideline of 1.5 mg/L. This study aimed at activating aluminosilicate clay soil mechanochemically, modifying aluminosilicate clay soil with Al-oxide and fabricating porous ceramic granules using Al-oxide modified mechanochemically activated aluminosilicate clay soil/ mechanochemically activated clay soil/ corn starch and evaluating their performances in defluoridation of groundwater. The raw clay materials were mechanochemically activated for 5, 10, 15 and 30 minutes for physicochemical transformation of the solid aggregate. The morphology of the samples showed the honeycomb structure. The surface area analyses of samples using Brunauer–Emmett–Teller (BET) gave the highest surface area of 50.5228 m2/g at 30 min activation time. Hence, the optimum activation time was 30 min. The Fourier Transform Infrared (FT-IR) analysis showed increase in the absorbance of FT-IR by Si-O-H groups at 510 cm-1 with increasing milling time. This is evidence that more surface Si-O-H groups were available at higher particle surface area that would be necessary to interact with fluoride. X-ray diffraction (XRD) analyses revealed that, at 30 minutes milling time, the peak broadening is intensified whereas the reflection peak intensities decreased. The X-ray fluorescence spectrometry (XRF) results for 30 minutes milling time showed that silica and alumina were the highest components in the clay soil. Using the activated clay in batch defluoridation of fluoride-spiked water, a maximum fluoride removal of 41% was achieved at a pHe of 2.41. The initial fluoride concentration was 9 mg/L while the sorbent dosage was 0.6 g/100 mL and the contact time being 30 minutes. The adsorption data fitted to both Langmuir and Freundlich isotherms. The adsorption data fitted only the pseudo-second-order kinetic, showing chemisorption. Optimization of Al3+ concentration for modification was carried out by modifying the mechanochemical activated aluminosilicate clay soil with different concentrations of Al3+ from which the optimum modification was achieved with 1.5 M. Characterisation studies on the Al-oxide modified mechanochemically activated aluminosilicate clay soil by SEM, BET, FT-IR, XRD and XRF, analyses were carried out to determine the resultant changes in physicochemical properties of the adsorbent owing to modification. The SEM image of Al-oxide modified mechanochemically activated clay soil showed many small pores and honey-comb structure on the surface of different images. The BET surface area and the BDH adsorption cumulative area of the Al-oxide modified mechanochemically activated v aluminosilicate clay soil were more than double those for the raw clay soil. There was also an increase in pore volume of the Al-oxide modified mechanochemically activated aluminosilicate clay soil. The FT-IR spectra showed that there was increase in the absorbance by the Si-OH, H-O-H, Al-O-H and Si-O-Al. The equilibrium pH of solution was higher than the point-of-zero charge (pHpzc) implying that fluoride removal occurred at solution pH > pHpzc where the net surface charge of the mechanochemically activated clay aluminosilicate soil was negative.The efficiency of 1.5 M Al-oxide modified aluminosilicate clay soil to remove fluoride from water was studied and found to be 96.5 % at pHe 6.86, contact time of 30 minutes and dosage of 0.3 g/100 mL for 10 mg/L fluoride solution at 200 rpm shaking speed. The result shows that Al-oxide modified mechanochemically activated aluminosilicate clay soil is effective for defluoridation. The adsorption data fitted to both Langmuir and Freundlich isotherms. The adsorption data fitted only the pseudo-second-order kinetic, showing chemisorption. Al-oxide modified mechanochemically activated aluminosilicate clay soil was tested for fluoride removal on field water and the percentage fluoride removal was 96.5 % at the dosage of 0.6 g/100 mL with the pHe of 6.48. The optimum Al-oxide modified mechanochemically activated aluminosilicate clay soil/ mechanochemically activated clay soil/ corn starch mixing ratio for fabrication of porous ceramic granules was determined by varying ratios and temperature. The optimum ratio found was 20:5:1.The porous ceramic granules were characterised using SEM, BET, FT-IR, XRD and XRF. SEM analysis showed that the porous ceramic granules have the porous structure of the organic foam template. The porous ceramic granule showed an increase in pore surface area and volume as compared to mechanochemically activated aluminosilicate clay soil. The FT-IR showed the presence of a strong broad bending and stretching vibrations band at about 993 cm-1 which shows the presence of Si–O–Si bonds. Mineralogical characterisation showed the presence of quartz, albite, horneblende and microcline as the main minerals of the calcined porous ceramic granules. The major oxides of the porous ceramic granules as shown by XRF analysis were SiO2, Al2O3, MnO and Na2O. The porous ceramic granules reduced the concentrations of fluoride in the water from 10 to 3.31 mg/L. The optimum adsorption capacity was 0.6648 mg/g at a pHe of 6.32 and the percentage fluoride removal was 66.9 % at an adsorbent dosage of 1.0063 g/100 mL and a temperature of 600 ⁰C. The porous ceramic granules were tested for fluoride removal on field water and the percentage fluoride removal was 45.4 % at the dosage of 1.0009 g/100 mL with the pHe of 7.87. Mechanochemically activated aluminosilicate clay soil showed higher adsorption capacity at acidic pH, therefore it is recommended that future work should focus on improving their adsorption capacity at wider range of pH. The porous ceramic granules can also be evaluated in column dynamic flow experiments.

MSc (Microbiology) / Department of Microbiology / See the attached abstract below

MESHWR / Department of Hydrology and Water Resources / Water scarcity has become a constraint for sustainable development in the higher education institutions in South Africa including University of Venda. Water infrastructure is one of the major challenges within higher education institutions, the existing water supply infrastructure is over 20 years old and was not planned for the current population. This study aimed at developing a water management plan for higher education institutions in South Africa using the University of Venda as a case study. A water resource management plan provides a road map for reducing water consumption while encouraging sustainable water utilisation. Evaluation was done within the institution to identify water sources, water resources infrastructure and water utilisation. The study employed questionnaire survey to collect data on water consumption, to evaluate issues of the institutional participation in resolving the water problems within the institution and deduce water wastage. Water supply data was obtained from meter readings and water invoices obtained from Vhembe District Municipality and University of Venda. The latter were used to determine water consumption within the institution. Based on the sample size, the results indicated the estimated amount of water consumed daily by UNIVEN population is 66 341.9 l/d excluding water utilised at the cafeteria, car wash and auditorium. The average water supplied to UNIVEN in 2017 was 67 642. 25 mega litres/month, this indicates that more water is supplied to the institution since the estimated institutional water demand excluding illegal students in the residence halls ranged between 415 740 l/d and 597 620 l/d of water while the estimated institutional water demand including illegal students varied between 282 2610 l/d and 406 6580 l/d. The latter further indicates that there is water wastage within the institution because the water supplied monthly to the institution was in mega litres as indicated in the invoices, but the results indicated that the amount of water utilised daily within the institution was in litres, this clearly shows that the respondents under estimated the amount of water they utilise. The average amount of water used by students residing on campus was 271 l/p/d to 735.5 l/p/d while day scholars and university staff use 55 l/p/d to 142.5 l/p/d. The average amount of water used for cleaning ranged between 1 318.5 l/d to 3 909 l/d while gardening usage ranged between 4600 l/d and 8 600 l/d. The School of Agriculture experimental farm uses 9 270.4 l/d and the university laundry was found to utilise 5 186 l/d. The university laboratories were found to utilise 125 l/d to 215 l/d per practical session with 3 to 4 practical sessions conducted per week. The study found that the total water used by construction workers for domestic purposes iv is 800 l/d. The utilisation trends showed that during the dry seasons, the university population generally used about twice as much water as compared to wet season because municipal water was supplemented by rain water for watering plants and washing the pavement. The survey results indicated that students residing in the university residences waste more water by allowing the tap to run while brushing teeth, washing dishes, excessive use of water to rinse clothes while doing laundry, opening showers and leaving the water running while waiting for a desired water temperature. Activities such as watering flowers and lawn in an unmonitored manner and pavement washing in which the hose pipe runs for several hours during watering and pavement washing result in water wastage. The university does not have an environmental or water education projects that encourage students and staff to use water efficiently. Water conservation measures are lacking in the institution because there are no policies and procedures that outline how water should be utilised. The developed water management plan for University of Venda outline the current water consumption, targets for reduction, drivers for reducing water consumption and water reduction strategies. This study recommends that the University targets to reduce potable water usage by 12% in the next 5 years. The latter has been highlighted in the proposed water management plan. The proposed plan further presents a suite of strategies to reduce water usage, improve institution water resources data and to plan for the future. Water utilisation within the institution has not been efficient due to water not being utilised in a sustainable manner. Sub-metering of each building, water awareness campaign, introduction of water utilisation policies, routine inspection and maintenance of infrastructure and appointing an environmental coordinator or working with water experts in the School of Environmental Sciences will help reduce water consumption within the institution. / NRF

MENVSC / Department of Geography and Geo-Information Sciences / Many rural communities of South Africa are living without adequate water supplies mainly due to historical lack of infrastructure and effective water reticulation systems. Day to day challenges of accessing water from distant boreholes and rivers are a reality particularly for women and children in rural Limpopo. This study investigates the nature and extent of water supply problems and how communities are living without adequate water in three rural communities of Greater-Giyani Local Municipality in South Africa. The study area lies in a semi-arid region which regularly experiences climate extremes such as droughts and floods which can reduce the ability of the municipality to supply water. Primary data was collected through questionnaires, key informant interviews and field observations while population, climate and hydrological data are also analyzed. A mixed methods research design was employed using qualitative methods such as content analysis whilst quantitative methods were dominated by time series analysis techniques and online interactive climate platforms such as the Climate Engine. It was found that households, schools and clinics in the study area rely mainly on boreholes for water supply but sometimes rivers supply those living nearby. An incomplete and poor water reticulation system coupled with erratic and shortening summer rainfall seasons are some of the major causes of water shortages in the study area. In order to cope with inadequate water, community members and public institutions in the study area have drilled boreholes and the sustainability of groundwater in the area is not well established. During summer, most households and institutions practise rainwater harvesting while a few resort to purchasing water from vendors. Despite these challenges which are not well documented, it was concluded that most of the rural poor households and institutions in the study area are well adapted to cope with water scarcity in the short term, while being vulnerable in the long term due to population growth and climate change. The study recommends the need for government and municipalities to invest in water reticulation systems in the long term whilst providing water to affected rural communities through water tankers, drilling more boreholes and maintenance of existing ones. Lessons learnt from this study may be useful to other municipalities across South Africa that are grappling with challenges of water access and supply. / NRF

MPH / Department of Public Health / Water quality of drinking water has been linked to good health outcomes across the world. The aim of this study was to assess physico-chemical, bacteriological, community practices regarding collection and storage of water and evaluation of human health risk characteristics of drinking water supplied by the government to Lufule village in Thulamela municipality, Limpopo Province, South Africa A cross-sectional study was conducted using questionnaires and interviews to determine drinking water handling practices and levels of contamination between the source and point-of-use at household. Assessment of water quality was carried out on 114 samples from selected sampling points using scientifically approved protocols. Total coliform was determined in 62.5% and 87.5% of the samples during the dry and wet seasons respectively. Similarly, E. coli was determined in 10.4 % and 13.2% in the dry and wet seasons, respectively. Trace metals levels in the drinking water samples were analysed and were within permissible range of both SANS and WHO. The calculated non-carcinogenic effects using hazard quotient toxicity potential, cumulative hazard index and chronic daily intake of drinking water through ingestion pathways were less than one unity, which showed that consumption of the water could pose little or no significant health risk. The results of this research suggest that lead has the potential of cancer risk to the residents through the cumulative ingestion in the drinking water samples of the studied area. Therefore, precaution needs to be taken to avoid potential risk of people in Lufule area especially, children. / NRF