White spot lesions (WSLs) or decalcifications remain a common complication in orthodontic patients with poor oral hygiene. The purpose of this study was to compare attitudes regarding the development of WSLs among patients, parents, orthodontists and general dentists and improve prevention and treatment protocols through better communication. A survey was developed to evaluate and compare the current opinions of orthodontic patients (n=315), parents (n=279), orthodontists (n=305) and general dentists (n=191) regarding the significance, prevention and treatment of WSLs. All four groups indicated that WSLs did detract from the overall appearance of straight teeth. All four groups indicated that patients were the most responsible for the prevention of WSLs. All four groups indicated that the general dentist should be more responsible for the treatment of WSLs than the orthodontist. General dentists were significantly more likely to indicate that the orthodontist was most responsible for the prevention of WSLs (P <0.005).

OBJECTIVES: To compare the amount of fluoride, calcium and phosphate release and recharge of a fluoride containing bioactive glass composite to a conventional resin composite and a resin modified glass ionomer cement at different time points. Furthermore, bond strength of a fluoride containing bioactive glass composite, a conventional flowable composite, and a resin modified glass ionomer cement to metal orthodontic brackets was evaluated. METHODS: A fluoride containing bioactive glass (BG) was synthesized using a sol-gel method and mixed homogeneously with an unfilled resin. For ion release and recharge, resin modified glass ionomer (RMGIC), Photac Fil Quick Aplicap (3M/ESPE) and flowable composite (Control), Filtek Supreme Ultra (Kerr), were used for comparison. Disc shape samples were fabricated using custom aluminum mold (1 mm in thickness and 9 mm in diameter, (n=5 for each material) and stored in 15 mL deionized water at 37°C until the testing time. The amounts of fluoride, calcium, and phosphate ions released were evaluated at different time points: 1 hour, 24 hours, 2 days, 3 days, 4 days, 5 days 6 days and 7 days. At each time point, all of the storage solution was extracted, and 7.5 mL was used for fluoride release measurement and the remaining 7.5 mL for calcium and phosphate ion release measurements. After solution extraction, the samples were replaced in 15 mL fresh deionized water at 37°C until the next sampling time point. Ionic recharge was performed with 5% sodium fluoride varnish (FluoroDose, Centrix) and MI paste plus (GC) following the ion release-testing period. An ion meter with a Fluoride ionic selective electrode were used to determine fluoride concentration. A Microwave-Plasma Atomic Emission Spectrometer (MP-AES) was used to test the concentration of the calcium and phosphate. For the shear bond strength test, rectangular shaped ceramic samples with the dimensions of 2 mm x 12 mm x 14 mm (Vita Mark II, Vita) were fabricated. Standard edgewise-metal brackets (American Orthodontics) were bonded to the center of the ceramic samples using tested material (n=10 for each material). Excess material was removed, and the cementing materials were polymerized from each side for 20 seconds. Specimens were either stored in water for 24 hours at 37o C or went under thermocycling for 5000 cycles. After the storage period, the specimens were subjected to shear bond strength test using an Instron universal machine at a crosshead speed of 0.5mm/min. Loads to failure were recorded to calculate shear bond strength. Comparison of released/recharged ions and shear bond strength were done by ANOVA and Tukey-Kramer HSD (α = 0.05) using JMP Pro 13. RESULTS: RMGIC showed significantly higher fluoride release and recharge than BG composite and the control. BG showed significantly higher Ca and P ion release compared to RMGIC followed by composite. RMGIC and BG showed significant ion recharge capability compared to composite. For the shear bond strength, the control composite showed significantly higher shear bond strength than BG composite followed by RMGIC. Thermocycling significantly increase bond strength for RMGIC and control but not for BG composite. CONCLUSIONS: 1. A fluoride containing bioactive glass composite was fabricated that showed the ability of ion release and recharge. 2. There was a significant difference in the amount of ion release and recharge among tested materials at different time points. 3. Favorable fluoride, calcium and phosphate ion release and recharge of BG composite were maintained over the testing period. 4. BG composite showed favorable bond strength to orthodontic metal brackets. 5. Thermocycling had a significant influence in bond strength for the materials tested except for BG composite. / 2020-07-18T00:00:00Z

A white spot lesion (WSL) is defined as enamel porosity, which could be due to an enamel defect or an initial carious lesion. ICON® resin is a resin infiltrant that penetrates into the enamel porosity and seals the lesion, thus inhibiting the progression of caries. This method is micro-invasive; however, the resin is radiolucent and the clinician cannot detect the material using radiographs. In order to develop a radiopaque resin, understanding the pore size and pore structure in WSLs is helpful. Therefore, the aim of this study was to characterise: (1) the pore size and structure, (2) the incorporation of radiopaque agents into ICON® resin. Brunauer-Emmett-Teller (BET) and focused ion beam-scanning electron microscopy (FIB-SEM) were used to characterise the porosity in WSLs. The data showed the enamel prisms become more pronounced in the advanced areas of the WSLs and demineralisation occurs within or/and between the enamel prisms. The pore size could be as small as an enamel crystallite, 28x48nm. Nano-strontium hydroxyapatite (non-coated and coated) and strontium bioglass were made as radiopaque fillers and characterised using different techniques including fourier transform infrared spectroscopy (FTIR), BET, transmission electron microscopy (TEM), X-ray diffraction (XRD) and particle size measurements. The radiopaque fillers had micron-sized particles, which made them unsuitable for infiltration into WSLs despite their possible ability to remineralise the WSLs. Radiopaque monomers including bromine-methacrylate and tin-methacrylate were able to make ICON® resin radiopaque and they showed a similar viscosity, wettability and biocompatibility compared to ICON® resin. The X-ray microtomography (XMT) showed that the experimental radiopaque resins were able to infiltrate into the WSLs, artificial and natural lesions, and they could be detected using image subtraction. Backscattered electron (BSE) imaging after each step of application of materials showed that the etching pattern and etchant gel could be barriers for a successful infiltration of the resins into WSLs.

Purpose: This in vitro study evaluated the effectiveness of using a 37% phosphoric acid liquid etchant along with MI Paste Plus™ powered technology compared to using MI Paste Plus™ alone or to an artificially created saliva solution in decreasing the demineralization and enhancing the remineralization of artificial carious lesions created on extracted human teeth. The teeth were analyzed and compared using polarized light microscopy, quantitative light-induced fluorescence, and digital photography. Materials and Methods: One hundred three recently extracted non-carious human third molar teeth without observable white-spot lesions, decalcification, or dental fluorosis were selected for this twelve day study and randomly divided into four treatment groups as follows: Group 1 (Control) - Artificial saliva solution (27 teeth) Group 2 (MIP) - MI Paste Plus™ application for 30 minutes daily for 12 days (26 teeth) Group 3 (15MIP) - 15 second etch every third day and MI Paste Plus™ application for 30 minutes daily for 12 days (25 teeth) Group 4 (1MIP) - 1 minute etch on day one ONLY and application of MI Paste Plus™ for 30 minutes daily for 12 days (25 teeth). Results: Results of one<–>way ANOVA revealed there was a significant effect for the type of treatment on the lesion depth (p = 0.0027). The post-hoc Tukey-Kramer's test indicated there was a statistically significant difference between the two groups (15MIP and 1MIP) that incorporated an acid etch in combination with MI Paste Plus™ and the group with exposure to MI Paste Plus™ alone (MIP). In addition, results of one<–>way ANOVA showed that there was no statistically significant effect for type of treatment on the change in fluorescence (p = 0.1417) or the change in density (p = 0.1934). Conclusions: The results of the present study revealed there was a significant effect for the type of treatment on the lesion depth (p = 0.0027). However, the only significant difference found was between the two groups (15MIP and 1MIP) that incorporated an acid etch in combination with MI Paste Plus™ and the group with exposure to MI Paste Plus™ alone (MIP). Thus, daily applications of MI Paste Plus™, with or without an acid etch, did not produce a statistically significant difference in mean lesion depth when compared to the control (artificial saliva group). In addition, the results of the present study showed that there was no statistically significant effect for type of treatment on the change in fluorescence (p = 0.1417) or the change in density (p = 0.1934). Further research is needed to evaluate MI Paste Plus™ capability in prevention of demineralization and/or enhancement of remineralization by conducting randomized clinical trials.

Pacientes ortodônticos apresentam grande prevalência de lesões de mancha branca devido ao maior acúmulo de biofilme em torno dos brackets. O objetivo deste estudo foi desenvolver um adesivo ortodôntico que apresentasse atividade antibacteriana e que estimulasse a deposição mineral para evitar o aparecimento e/ou a evolução destas lesões. Adesivos experimentais foram formulados contendo 75% de BisGMA e 25% de TEGDMA por peso; sistema fotoiniciador a base de canforoquinona e 5% de sílica coloidal. Os compostos 1,3,5-triacriloilhexahidro-1,3,5-triazina (TAT) e vidro fosfato invertido contendo pentóxido de nióbio (PIG-Nb) foram adicionados ao adesivo base, como agente antibacteriano e remineralizante, respectivamente. Foram analisados grupos contendo somente TAT nas concentrações de 10, 15 e 20% por peso; grupos contendo somente PIG-Nb nas concentrações de 1, 2,5 e 5% por peso e um grupo contendo 20% TAT e 5% PIG-Nb, por peso. Utilizou-se um grupo sem a adição de TAT e PIG-Nb como Grupo Controle, dentre os experimentais. O adesivo ortodôntico comercial Transbond XT foi utilizado para comparação. Avaliou-se a atividade antibacteriana, capacidade de deposição mineral, variação de pH, grau de conversão, amolecimento em solvente e resistência de união dos adesivos ortodônticos. Os adesivos desenvolvidos apresentaram atividade antibacteriana e capacidade de estimular deposição mineral sem prejudicar as propriedades dos adesivos. Os resultados obtidos a partir dos ensaios realizados neste trabalho permitem concluir que se trata de material com propriedades favoráveis e potencialidade de, após ajustes necessários, tornar-se viável para uso na prática odontológica. / White spot lesions are a concern for orthodontic patients due to biofilm accumulation around brackets. The aim of this study was to develop an antibacterial and remineralizing orthodontic adhesive to overcome these lesions. Experimental orthodontic adhesives were formulated containing 75wt% BisGMA and 25wt% TEGDMA; camphorquinone-based photoinitiator system and 5wt%fummed silica. The compounds 1,3,5-tryacryloylhexahydro-1,3,5-triazine (TAT) and phosphate invert glass containing niobium pentoxide (PIG-Nb) were added to the adhesive as antibacterial and remineralizing agents, respectively. Experimental groups obtained containing only TAT at 10, 15 and 20wt% concentration; groups containing only PIG-Nb at 1, 2.5 and 5wt%, and one group containing 20wt% TAT and 5wt% PIG-Nb. A groups without TAT and PIG-Nb was used as Control and Transbond XT was used as a commercial control. Antibacterial activity, mineral deposition, changes in pH, degree of conversion, softening in solvent and shear bond strength of orthodontic adhesives was assessed. The experimental adhesives presented antibacterial activity and were capable to induce mineral deposition with no negative effect on adhesive's properties. The results of this study allow the conclusion that this material has favorable properties and potential, after necessary adjustments become viable for use in dental practice.

Pacientes ortodônticos apresentam grande prevalência de lesões de mancha branca devido ao maior acúmulo de biofilme em torno dos brackets. O objetivo deste estudo foi desenvolver um adesivo ortodôntico que apresentasse atividade antibacteriana e que estimulasse a deposição mineral para evitar o aparecimento e/ou a evolução destas lesões. Adesivos experimentais foram formulados contendo 75% de BisGMA e 25% de TEGDMA por peso; sistema fotoiniciador a base de canforoquinona e 5% de sílica coloidal. Os compostos 1,3,5-triacriloilhexahidro-1,3,5-triazina (TAT) e vidro fosfato invertido contendo pentóxido de nióbio (PIG-Nb) foram adicionados ao adesivo base, como agente antibacteriano e remineralizante, respectivamente. Foram analisados grupos contendo somente TAT nas concentrações de 10, 15 e 20% por peso; grupos contendo somente PIG-Nb nas concentrações de 1, 2,5 e 5% por peso e um grupo contendo 20% TAT e 5% PIG-Nb, por peso. Utilizou-se um grupo sem a adição de TAT e PIG-Nb como Grupo Controle, dentre os experimentais. O adesivo ortodôntico comercial Transbond XT foi utilizado para comparação. Avaliou-se a atividade antibacteriana, capacidade de deposição mineral, variação de pH, grau de conversão, amolecimento em solvente e resistência de união dos adesivos ortodônticos. Os adesivos desenvolvidos apresentaram atividade antibacteriana e capacidade de estimular deposição mineral sem prejudicar as propriedades dos adesivos. Os resultados obtidos a partir dos ensaios realizados neste trabalho permitem concluir que se trata de material com propriedades favoráveis e potencialidade de, após ajustes necessários, tornar-se viável para uso na prática odontológica. / White spot lesions are a concern for orthodontic patients due to biofilm accumulation around brackets. The aim of this study was to develop an antibacterial and remineralizing orthodontic adhesive to overcome these lesions. Experimental orthodontic adhesives were formulated containing 75wt% BisGMA and 25wt% TEGDMA; camphorquinone-based photoinitiator system and 5wt%fummed silica. The compounds 1,3,5-tryacryloylhexahydro-1,3,5-triazine (TAT) and phosphate invert glass containing niobium pentoxide (PIG-Nb) were added to the adhesive as antibacterial and remineralizing agents, respectively. Experimental groups obtained containing only TAT at 10, 15 and 20wt% concentration; groups containing only PIG-Nb at 1, 2.5 and 5wt%, and one group containing 20wt% TAT and 5wt% PIG-Nb. A groups without TAT and PIG-Nb was used as Control and Transbond XT was used as a commercial control. Antibacterial activity, mineral deposition, changes in pH, degree of conversion, softening in solvent and shear bond strength of orthodontic adhesives was assessed. The experimental adhesives presented antibacterial activity and were capable to induce mineral deposition with no negative effect on adhesive's properties. The results of this study allow the conclusion that this material has favorable properties and potential, after necessary adjustments become viable for use in dental practice.

Pacientes ortodônticos apresentam grande prevalência de lesões de mancha branca devido ao maior acúmulo de biofilme em torno dos brackets. O objetivo deste estudo foi desenvolver um adesivo ortodôntico que apresentasse atividade antibacteriana e que estimulasse a deposição mineral para evitar o aparecimento e/ou a evolução destas lesões. Adesivos experimentais foram formulados contendo 75% de BisGMA e 25% de TEGDMA por peso; sistema fotoiniciador a base de canforoquinona e 5% de sílica coloidal. Os compostos 1,3,5-triacriloilhexahidro-1,3,5-triazina (TAT) e vidro fosfato invertido contendo pentóxido de nióbio (PIG-Nb) foram adicionados ao adesivo base, como agente antibacteriano e remineralizante, respectivamente. Foram analisados grupos contendo somente TAT nas concentrações de 10, 15 e 20% por peso; grupos contendo somente PIG-Nb nas concentrações de 1, 2,5 e 5% por peso e um grupo contendo 20% TAT e 5% PIG-Nb, por peso. Utilizou-se um grupo sem a adição de TAT e PIG-Nb como Grupo Controle, dentre os experimentais. O adesivo ortodôntico comercial Transbond XT foi utilizado para comparação. Avaliou-se a atividade antibacteriana, capacidade de deposição mineral, variação de pH, grau de conversão, amolecimento em solvente e resistência de união dos adesivos ortodônticos. Os adesivos desenvolvidos apresentaram atividade antibacteriana e capacidade de estimular deposição mineral sem prejudicar as propriedades dos adesivos. Os resultados obtidos a partir dos ensaios realizados neste trabalho permitem concluir que se trata de material com propriedades favoráveis e potencialidade de, após ajustes necessários, tornar-se viável para uso na prática odontológica. / White spot lesions are a concern for orthodontic patients due to biofilm accumulation around brackets. The aim of this study was to develop an antibacterial and remineralizing orthodontic adhesive to overcome these lesions. Experimental orthodontic adhesives were formulated containing 75wt% BisGMA and 25wt% TEGDMA; camphorquinone-based photoinitiator system and 5wt%fummed silica. The compounds 1,3,5-tryacryloylhexahydro-1,3,5-triazine (TAT) and phosphate invert glass containing niobium pentoxide (PIG-Nb) were added to the adhesive as antibacterial and remineralizing agents, respectively. Experimental groups obtained containing only TAT at 10, 15 and 20wt% concentration; groups containing only PIG-Nb at 1, 2.5 and 5wt%, and one group containing 20wt% TAT and 5wt% PIG-Nb. A groups without TAT and PIG-Nb was used as Control and Transbond XT was used as a commercial control. Antibacterial activity, mineral deposition, changes in pH, degree of conversion, softening in solvent and shear bond strength of orthodontic adhesives was assessed. The experimental adhesives presented antibacterial activity and were capable to induce mineral deposition with no negative effect on adhesive's properties. The results of this study allow the conclusion that this material has favorable properties and potential, after necessary adjustments become viable for use in dental practice.

A thesis submitted to the College of Dental Medicine of Nova Southeastern University of the degree of Master of Science in Dentistry. Objective: This study was conducted to determine the resistance to wear and effectiveness of Opal®Seal (Opal Orthodontics by Ultradent, South Jordan, UT, USA) against enamel demineralization in comparison to L.E.D. Pro Seal® (Reliance Orthodontic Products, Itasca, IL, USA). Background: Development of white spot lesions (WSL) is a primary concern during fixed orthodontic treatment. With poor oral hygiene during orthodontic therapy, it is almost inevitable that enamel demineralization will occur and WSLs will be seen clinically. Numerous materials have demonstrated successful prevention of WSLs, including topical varnishes and sealants, gels, pastes and bonding agents. The newly developed sealant Opal Seal is claimed by the manufacturer to prevent demineralization and subsequent WSLs from forming during orthodontic treatment. To evaluate the effectiveness of Opal Seal, we tested and compared it to Pro Seal, which has proven to be successful at WSL prevention and is, like Opal Seal, a fluoride-releasing, light-cured, low viscosity filled resin sealant. Methods: A total of 48 non-carious extracted human premolar teeth were divided into 3 groups representing one of the following topical treatments: no treatment (C), Opal Seal (OS) or L.E.D. Pro Seal (PS). Each group was subdivided into either T1 or T2 time interval groups (C1, C2, OS1, OS2, PS1 and PS2) and teeth were subjected to 10,000 or 20,000 simulated brush strokes, respectively, followed by exposure to an acidic solution for 96 hours. Teeth were examined macroscopically for product wear and assessment of WSL development and then sectioned for quantitative examination with polarized light microscopy. Results: Visual assessment revealed wear of L.E.D. Pro Seal in 62.5% of the PS2 teeth following exposure to toothbrush abrasion and acidic challenge. No L.E.D. Pro Seal or Opal Seal wear was found in any PS1 or OS teeth, respectively. WSLs involving 50-100% of the exposed enamel surface developed in all control teeth and 37.5% of PS teeth had WSLs involving less than 50% of their enamel surface. These WSLs were visible as small, white, isolated points. No visible WSLs were found in any teeth in the OS group. A Fisher's Exact test was used to evaluate any differences in demineralization within groups over time. The results indicate a significant difference in the number of lesions found at T1 compared to T2 in the PS group (p = 0.003). A non-parametric Kruskal-Wallis test using a Wilcoxon test for all multiple comparisons was conducted to evaluate any differences among the treatment groups for change in depth of demineralization (µm) at two different time points. PS and OS groups were found to have significant differences in average lesion depth compared to the control groups at T1 and T2 (pConclusions: Our results showed that both Opal Seal and L.E.D. Pro Seal reduce enamel demineralization when teeth are subjected to simulated toothbrush abrasion and an acidic environment over time. Opal Seal provided superior protection of the enamel surface and demonstrated complete wear resistance and prevention of demineralized lesion development in our experimental groups.

White Spot lesions are a common iatrogenic occurrence on patients who are treated with fixed orthodontic appliances. There is a dynamic chemical interaction between enamel and saliva at the tooth surface that allow a lesion to have phase changes involving demineralization of enamel and remineralization. This is due to calcium and phosphate dissolved in saliva that is deposited onto the tooth surface or removed depending on the surrounding pH. Caseinphosphopeptide-amorphous calcium phosphate (CPP-ACP) is gaining popularity in dentistry as a way to increase the available level of calcium and phosphate in plaque and saliva to improve the chemical gradient so that if favors remineralization. The aim of our investigation is to search the available current literature and formulate a recommendation for use of CPP-ACP in orthodontics. Publications from the following electronic databases were searched: PubMed, Web of Science, Cochrane Library and Science Direct. Searches from August 2010 to April 1st 2012 were performed under the terms "MI Paste OR Recaldent OR caseinphosphopeptide-amorphous calcium phosphate OR CPP-ACP or tooth mousse". The searches yielded 155 articles, These were reviewed for relevance based on inclusion and exclusion criteria. Articles with inappropriate study design or no outcome measures at both baseline and end point were also excluded. 13 articles were deemed of relevance with a high quality study design and were included in this study for evaluation. The current literature suggests a preventative treatment regimen in which MI Paste Plus is used. It should be delivered once daily prior to bed after oral hygiene for 3 minutes in a fluoride tray, throughout orthodontic treatment. It should be recommended for high risk patients determined by poor oral hygiene, as seen by the inability to remove plaque from teeth and appliances. This protocol may prevent or assist in the remineralization of enamel white spot lesions during and after orthodontic treatment. / Oral Biology

Submitted by MARIA ÂNGELA LACERDA RANGEL ESPER null (angela_esper@hotmail.com) on 2016-04-13T16:41:11Z No. of bitstreams: 1 TESE FINAL ANGELA 2016.pdf: 1673462 bytes, checksum: 45fa78583c51eb4cc460fab26a8a4fc5 (MD5) / Approved for entry into archive by Felipe Augusto Arakaki (arakaki@reitoria.unesp.br) on 2016-04-14T20:50:08Z (GMT) No. of bitstreams: 1 esper_malr_dr_sjc.pdf: 1673462 bytes, checksum: 45fa78583c51eb4cc460fab26a8a4fc5 (MD5) / Made available in DSpace on 2016-04-14T20:50:08Z (GMT). No. of bitstreams: 1 esper_malr_dr_sjc.pdf: 1673462 bytes, checksum: 45fa78583c51eb4cc460fab26a8a4fc5 (MD5) Previous issue date: 2016-02-16 / O trabalho in vitro avaliou a eficácia da inativação fotodinâmica (PDI) da eritrosina (E) e hematoporfirina IX (H), com 10 µM, utilizando LED azul, dose de 75 J/cm2 em células planctônicas e biofilme de S. mutans (UA 159). Suspensões padrões contendo 107 células/mL foram preparadas e submetidas a diferentes condições experimentais: a) hematoporfirina IX e LED (H+L+); b) eritrosina e LED (E+L+); c) apenas LED (F-L+); d) tratamento somente com hematoporfirina IX (H+L-); e) somente com eritrosina (E+L-); e f) grupo controle, sem tratamento com fotossensibilizador (F) e sem a utilização de LED (F-L-). As cepas foram semeadas em ágar MSBS para contagem de unidades formadoras de colônias (UFC/mL). Na segunda parte do trabalho foi realizado a PDI em biofilme de S. mutans sobre bráquetes metálicos e cerâmicos, com H a 10 µM e LED azul. Os resultados foram submetidos à análise de variância e teste de Tukey (p<0,05) e demonstraram que a E sob efeito do LED (E+L+) não foi eficaz na PDI de células planctônicas, nos parâmetros usados (p=0,3644). No entanto, a H promoveu redução de 6,78 log10 (p<0,0001), no grupo de tratamento (H+L+). A PDI com a associação da H e LED foi efetiva na redução de 100% de culturas planctônicas de S. mutans, porém o mesmo não foi observado na associação com a E, na dosimetria utilizada no experimento. A PDI no biofilme de S. mutans sobre bráquetes metálicos, com a H e LED não foi eficaz nos parâmetros utilizados (p=0,1023), no entanto, ocorreu diminuição significativa de 53% sobre bráquetes cerâmicos (p=0,004). A H IX modificada é promissora como agente fotossensibilizador a ser empregado na técnica de PDI em associação ao LED azul, sendo necessários outros ensaios, em novas concentrações e/ou dosimetrias para se conseguir a inativação bacteriana. / The in vitro study evaluated the efficacy of photodynamic inactivation (PDI) with erythrosine (E) and hematoporphyrin (H) 10 µM, using a blue light-emitting diode (LED), a fluence of 75 J/cm2 , on planktonic cultures and biofilm of S. mutans (UA 159). Suspensions containing 107 cells/mL were prepared and were tested under different experimental conditions: a) hematoporphyrin IX and LED (H+L+); b) erythrosine and LED irradiation (E+L+); c) only LED (P-L+); d) only hematoporphyrin IX (H+L-); e) only erythrosine (E+L-); and f) control group, no LED irradiation or photosensitizer (P) treatment (P-L-). After treatment, the strains were seeded onto MSBS agar in order to determine the number of colony-forming units (CFU/mL). The second part of this work consisted of the PDI of S. mutans biofilm on metal and ceramic brackets with the H 10 μM and blue LED. The results were submitted to analysis of variance and the Tukey test (p<0.05) and showed that E under the effect of LED proved to be ineffective in the PDI of planktonic cultures with the parameters used (p=0.3644). H, however, caused a reduction of 6.78 log10 (p<0.0001) in the treatment group (H+L+). PDI with H and LED exerted antimicrobial effect of 100% of the S. mutans strain studied, whereas the same was not observed in the association with E in the dosimetry used in this work. PDI on S. mutans biofilm on metal brackets, with H and LED was not effective with the parameters used (p=0.1023), however on ceramic brackets caused a significant reduction of 53% (p=0,004). Modified H IX is a promising photosensitizer to be used in the PDI technique in combination with blue LED. Therefore, new tests with new concentrations and/or dosimetry are needed to achieve bacterial inactivation.