There is little information published on red sorrel (Rumex acetosella L.), a perennial weed that is considered a serious problem in wild blueberry production. Hexazinone, a photosystem II inhibitor, has been used in wild blueberry fields for more than 30 years. Hexazinone efficacy on red sorrel has declined over time. Therefore, a two year study was conducted to examine hexazinone alternatives that can be sprayed in wild blueberry fields. Red sorrel ramets from mature blueberry fields were tested to determine whether long-term spraying of hexazinone selected for resistant red sorrel. The results show that hexazinone+rimsulfuron/nicosulfuron may be a alternative for hexazinone. Red sorrel from some blueberry fields is hexazinone-resistant and the resistance is caused by a Phe255 to Val mutation in the psbA gene.

Weeds are considered a major problem for organic wild blueberry (Vaccinium angustifolium Ait.) production due to their excessive growth and limited management options available. Therefore, the primary objective of this study was to investigate burning in combination with sulphur application as a weed management option for organic blueberry production. In addition, Finalsan® and EcoclearTM as potential candidates for perennial weed control in organic production were evaluated. Burned and sulphur plots produced higher blueberry stem density, blueberry cover and yield compared to mowed and no-sulphur plots, respectively, although, the interaction was not significant. Similarly, low weed density and cover were observed in burning and sulphur application plots. Mycorrhizal colonization in blueberry roots was significantly higher in burned plots than mowed plots. A single application of Finalsan® and EcoclearTM effectively controlled goldenrod, sweet fern and bracken fern. However, a single application of either product did not control wild raisin or moss.

The heavy rainfall, gentle to severe topography with high proportion of bare spots, and weed patches emphasize the need of variable rate split (VRS) fertilization in wild blueberry. Two commercial fields were selected in central Nova Scotia to evaluate the impact of VRS fertilization on ammonia volatilization, subsurface water quality, and crop productivity. Management zones were delineated based on slope variability, and different fertilizer rates were applied using global positioning system (GPS) guided prescription map. Ammonia huts were used to quantify the ammonia volatilization losses, while the lysimeters were installed in the fields to evaluate the impact of different fertilizer treatments on subsurface water quality. The VRS treatment significantly decreased the ammonia volatilization losses and nutrients leaching losses as compared to uniform treatment. Based on the results of this study, it can be concluded that VRS fertilization in wild blueberry fields could reduce environmental contamination and improve crop productivity.

This thesis consists of three sections that provide detailed knowledge of nutrient estimation and management in wild blueberry production. The first section investigated the main and interactive effects of long term fertilizer (NPK) enrichments on soil mineral nitrogen, organic nitrogen and carbon, microbial biomass nitrogen and carbon, net mineralization and net nitrification in wild blueberry soils. The second section studied the optimization of wild blueberry growth, development, foliar nutrients and harvestable yields by using response surface methodology. The third section examined nutrient estimation technologies using field spectroscopy. The remote sensing data was analysed with a combination partial least squares regression and variable selection algorithms (Chemometric analysis). The results indicated elevated nitrification activity under nitrogen enrichments, mainly performed by heterotrophs, report unusually high levels of dissolved organic carbon (> 150 C ha-1), a fungal dominated soil system and high concentration of soluble organic nitrogen in the crop year of production. Nitrification and high dissolved organic carbon levels were observed in connection with possible nitrogen saturation and potential environmental hazards. The results imply a need for nitrification inhibition measures. Results from field studies examining the main and interactive effects of soil applied N, P and K suggested that applications of nitrogen (35 kg ha-1), phosphorus (40 kg ha-1) and potassium (30 kg ha-1) were required to optimize growth, development and harvestable yields of wild blueberry. Under these fertilizer rates, the corresponding predicted harvestable yield was 4,126 kg ha-1 that is as much as 13% higher than would be produced by commonly used fertilizer rate in the industry. This study presented new leaf nutrient ranges for sprout and crop years for wild blueberry fields in Atlantic Canada. Hyperspectral remote sensing technologies were used for estimating macro and micro nutrients. This study provides critical information on wavelengths important for nutrient estimation in reflectance spectra (400-2500 nm). The results and inferences from this thesis may be employed to improve crop production, increase economic returns and health of soil and sustainability of wild blueberry production in Nova Scotia. / This study was undertaken to examine the response of the wild blueberry plant to soil applied fertilizers and encompasses soil nitrogen and carbon pools, plant growth and development, leaf nutrient concentrations and harvestable yields. In addition, given the vast area in which wild blueberry fields are located, the study also examined the feasibility of assessing plant nutrient status through the use of remote sensing hyperspectral technologies. Our results emphasize the importance of monitoring for soil nitrogen and carbon pools in the context of accelerated nitrogen cycling, nitrogen saturation, the fine-tuning of current leaf nutrient ranges in Atlantic Canada in connection to fertilizer rates, the possibility of estimating leaf nutrient contents by remote sensing technologies all with the aim of optimizing wild blueberry yields. In terms of statistical techniques, this thesis used response surface methodologies with a central composite design as a means of discovering, the main and interactive effects of soil applied fertilizers to determine the most appropriate soil nitrogen levels and leaf nutrient ranges that correlate to the highest harvestable yields. The remote sensing data used to estimate leaf nutrients concentrations, various models that combined chemometrics and response surface methodologies for determining model efficiencies with aim of getting informative wavelengths in wild blueberry fields.

The variable rate fertilizer spreader was modified to control each pair of nozzles for spot-application of fertilizer only in plant areas of wild blueberry fields. The experiments were conducted to evaluate performance accuracy of modified variable rate granular (MVRG) fertilizer spreader. The results suggested that the MVRG fertilizer spreader performed efficiently in detecting bare spots/weed patches and clay filler application only in green grass/plant areas. Two wild blueberry fields were selected to evaluate the impact of MVRG spreader on nutrient leaching through small bare spots/weed patches. Management zones were delineated on the basis of slope variability. The MVRG spreader significantly reduced the nutrient loading in subsurface water samples collected from the bare spots/weed patches. Based on the results obtained, it can be concluded that the fertilization in wild blueberry fields using MVRG fertilizer spreader can result in the protection of subsurface water quality, thus protecting the environment.

Two wild blueberry fields were selected in central Nova Scotia, to characterize and quantify the spatial pattern of variability in soil properties, leaf nutrients and fruit yield, identification of yield influencing soil properties, and to develop management zones for site-specific fertilization. A combination of classical statistics, geostatistical analysis and mapping in Arc GIS 9.3 indicated substantial variation within field. The stepwise regression suggested that the soil EC, horizontal co-planar geometry (HCP), inorganic nitrogen and moisture content were major yield influencing factors. The cluster analysis of the soil variables with the fruit yield also indicated that HCP, inorganic nitrogen, EC, SOM, and ?v were closely grouped with the fruit yield at a similarity level greater than 70%. Based on the results of this study the wild blueberry fields can be divided into different management zones for variable rate fertilization to improve crop production, increase revenue, and reduce potential environmental contamination.

An automated prototype variable rate (VR) sprayer was developed for control of eight individual nozzles on a 6.1 m sprayer boom for spot-application (SA) of agrochemicals in wild blueberry fields. The VR control system consisted of eight ultrasonic sensors and four cameras mounted on a separate boom in front of the ATV, flow controller, VR controller, ruggedized computer, flow valve and solenoid valves. Custom software was capable of processing the images to detect weeds or plants in real-time and automatically send a signal to the VR controller for SA at the correct target. The performance of VR sprayer for SA on weeds (herbicide) and foliage (fungicide) was evaluated in commercial wild blueberry fields. Based on the results of this study, the VR sprayer was reliable, efficient and accurate enough for SA of agrochemicals. The average volume of chemical saved with SA was 70 % herbicide and 30 % fungicide.

Two wild blueberry fields were selected to evaluate the impact of variable rate (VR) fertilization on crop productivity, surface and subsurface water quality. Management zones were delineated based on slope variability, and different fertilizer rates were applied according to prescription maps. Runoff collectors were place in the fields to measure the nutrient losses in surface runoff, while lysimeters were installed to evaluate the impact of VR fertilization (VRF) on subsurface water quality. The VR treatment significantly decreased phosphorus and nitrogen loadings in surface runoff as compared to uniform treatment. The concentrations of nutrients in subsurface water samples were also significantly lower for VR treatment as compared to uniform treatment. The excessive nutrients enhanced vegetative growth in low lying areas of uniform fertilization, while berry yield was less. Based on these results, it can be concluded that VRF in wild blueberry fields improved the crop productivity and potential environmental impacts. / This study was conducted to evaluate the impact of variable rate fertilization on crop productivity and surface and subsurface water quality in wild blueberry fields. Result illustrated that variable rate fertilization significantly reduce the nutrients loading in surface and subsurface water, and improved blueberry yield.

Le bleuet sauvage (Vaccinium angustifolium Ait. et V. myrtilloides Michaux) représente un marché en plein essor au Canada, premier pays producteur et exportateur mondial de ce fruit. Pour faire face à la demande, les producteurs cherchent continuellement à adapter leurs pratiques de production dans le but d’améliorer leur rendement et l'état de santé de leurs bleuetiers. Or, les micro-organismes présents dans les racines et dans le sol jouent un rôle non négligeable en lien avec la santé des plantes. Ce microbiote est donc d’intérêt d’un point de vue agronomique, pourtant, contrairement à d’autres cultures, très peu d’études se sont penchées spécifiquement sur le microbiote du milieu racinaire du bleuetier sauvage. Ce doctorat s’inscrit donc dans l’optique d’accroître les connaissances sur les communautés bactériennes et fongiques présentes dans les bleuetières au Québec. Les objectifs de ce projet sont de détecter les taxons qui pourraient avoir un impact sur les variables agronomiques des bleuetiers telles que le rendement; d’identifier les variables physico-chimiques du sol influençant ces communautés; et d’étudier les impacts que peuvent avoir les différentes pratiques agricoles, telles que la fertilisation et la fauche thermique, sur ces micro-organismes. Nous nous sommes appuyés sur le séquençage de nouvelle génération et le métacodage à barres de l’ADN environnemental de nos échantillons de racines et de sol afin d’obtenir une analyse des communautés bactériennes et fongiques de la rhizosphère et des racines des bleuetiers. Les analyses multivariées effectuées par la suite permettent de comparer ces communautés et de voir si certaines espèces sont spécifiques à une condition particulière. Dans l’ensemble, cette thèse a donc permis de caractériser les communautés fongiques et bactériennes du milieu racinaire du bleuetier sauvage in situ dans plusieurs bleuetières du Québec. De nombreuses espèces de champignons mycorhiziens éricoïdes ont été systématiquement identifiées dans les trois études et leur prédominance suggère leur importance pour le bleuetier sauvage. Nous avons également trouvé que l’ordre bactérien des Rhizobiales, connu pour sa capacité à fixer l’azote atmosphérique, occupait une part importante de la communauté bactérienne. Les études sur la fertilisation et la fauche thermique ont démontré que ces deux pratiques agricoles avaient peu d’impact significatif sur les communautés microbiennes étudiées. Enfin, cette thèse donne des pistes de réflexion sur la fixation d’azote par les communautés bactériennes et pose les premières bases pour des essais de bio-inoculation avec les espèces fongiques et bactériennes détectées ayant un potentiel impact bénéfique sur la culture des bleuets sauvages. / The wild blueberry (Vaccinium angustifolium Ait. and V. myrtilloides Michaux) market is booming in Canada, the world's leading producer and exporter of this fruit. In order to meet the demand, growers are constantly trying to adapt their production practices to improve their yields and the health of their blueberry fields. Micro-organisms present in the roots and in the soil play a significant role in the health of the plants. This microbiota is therefore of interest from an agronomic point of view, yet, contrary to other crops, very few studies have been conducted specifically on the microbiota of the root environment of wild blueberries. This doctoral project therefore aims at increasing our knowledge of the bacterial and fungal communities present in wild blueberry fields in Quebec. The objectives of this project are to detect taxa that could have an impact on agronomic variables of wild blueberry fields such as fruit yield; to identify soil physico-chemical variables influencing these communities; and to study the impacts that different agricultural practices, such as fertilization or thermal pruning, may have on these micro-organisms. We relied on next generation sequencing and metabarcoding of environmental DNA from our root and soil samples to obtain an analysis of the bacterial and fungal communities in the rhizosphere and roots of blueberry shrubs. Subsequent multivariate analyses allow us to compare these communities and see if certain species are specific to a particular condition. Overall, this thesis has characterized the fungal and bacterial communities in the root environment of wild blueberry in situ in several Quebec wild blueberry fields. Numerous species of ericoid mycorrhizal fungi were systematically identified in all three studies, and their predominance suggests their importance to wild blueberries. We also found that the bacterial order Rhizobiales, known for its ability to fix atmospheric nitrogen, occupied an important part of the bacterial community. Studies on fertilization and thermal mowing showed that these two agricultural practices have limited significant impacts on the microbial communities studied. Finally, this thesis provides insights into nitrogen fixation by bacterial communities and lays the groundwork for bio-inoculation trials with the fungal and bacterial species detected to have a potential beneficial impact on wild blueberry cultivation.