Winter wheat has several advantages over spring varieties, higher (25 % more) yield, efficient use of spring moisture, reduction of soil erosion by providing ground cover during the fall and early spring, rapid initial spring growth to out - compete weeds and circumvent the peak of Fusarium head blight infections by flowering early. Winter wheat is planted in early autumn when it germinates and developing seedlings acclimate to cold. The crown survives under snow cover and in spring rapidly grows into a vigorously growing plant for grain to be harvested in summer. However, the harsh Canadian prairie winters require that winter wheat has increased cold hardiness and improved winter survival to reduce losses from sudden cold snaps during winter and spring. Low temperature (LT) tolerance is one of the major components of cold hardiness. Genetic mapping studies have revealed a major quantitative trait locus (Fr-A2) at wheat chromosome 5A which can explain at least 50 % of LT tolerance in wheat. Physical mapping of 5A LT QTL in a hardy winter wheat cv Norstar revealed a cluster of at least 23 C - repeat binding factors (CBF) coinciding with peak of Fr-A2 QTL. The objective of this study is biochemical, and molecular characterization of CBF co - located at Fr-A2 to identify key CBF participating in conferring LT tolerance in winter wheat. A comparative analysis of CBF gene cluster at the Fr-A2 collinear region among Poaceae members showed an expansion in the number of CBF genes with increased LT tolerance. Rice, a cold sensitive member, had only three CBF genes, whereas cold hardy winter wheat cv Norstar has 23 CBF genes. Amino acid sequence - based cluster analysis of complete CBF genes, or their major functional components such as the AP2 - DNA binding domain and C - terminal trans - activation domain, divide Norstar CBF into Pooideae specific clades. However, analyses of Norstar CBF amino acid sequences of different functional groups revealed a shift in clade members. These results suggest divergence of CBF functions which could lead to possible differences / similarity in the regulon activated by a CBF in a specific group. The 15 CBF genes from winter wheat cv Norstar were expressed in E. coli to produce recombinant TrxHisS - CBF fusion proteins in adequate quantities for structural and functional assays. All CBF fusion proteins could be recovered in the E. coli soluble phase of cell extract, except that the CBF17.0 fusion protein could only be recovered with 6 M urea extraction. Eleven of the 15 CBF fusion proteins were very stable in heat (98 oC), 10 % SDS and 6 M urea treatment. The five other CBF members were very labile under native conditions, but were stable in E. coli cell extracts or when extracted under denaturing conditions. Most of the CBF recombinant proteins in denaturing gel electrophoresis migrated slower than expected from their predicted molecular mass, based on amino acid sequence. The slow migration could be associated to their elongated protein structure as determined by dynamic light scattering (DLS). CBF 12.2 and CBF 17.0 were highly resistant to denaturation and retained their secondary structure in these conditions as determined by circular dichroism (CD) spectra. The high stability of these two CBF proteins may be important for cold acclimation or maintenance of cold hardiness in wheat. CBF proteins are transcription factors that bind to the dehydration-responsive element / C-repeat element (DRE / CRT) motif (CCGAC). Ten of the 15 Norstar recombinant CBFs whether purified under native or denaturing conditions showed in vitro binding to the CRT motif. Within hours of cold exposure (4 oC) the native CBF increased their affinity to CRT interaction which could be due to changes in the CBF secondary structures. Some of the CBF for binding preferred the core GGCCGAC motif while others preferred TGCCGAC. Similarly binding assays with truncated CBF revealed that for some CBF proteins, the second signature motif (DSAWR) and remaining C - terminal were not needed, while for others a considerable portion of the C -terminal region was needed for binding. Norstar CBF 12.1 has a memory of cold experience, and upon exposure to cold, has a high and immediate affinity to CRT elements. A homolog CBF12.2 in less cold - hardy winter wheat cv Cappelle - Desprez had a non - functional protein due to a R → Q substitution in a highly conserved residue within the AP2 domain. Several of the cv Norstar CBFs showed increased activity under LT and denaturing conditions, which may be the reason for the greater cold hardiness in Norstar. In conclusion, detailed and extensive analyses of CBF in this study characterized their structure and function relationships, which are important for understanding and improving LT tolerance in plants. The identification of specific CRT binding motifs and two CBFs which were very stable under adverse conditions may be prime candidates for further study to improve LT tolerance in plants.

Doctor of Philosophy / Department of Entomology / C. Michael Smith / The wheat curl mite, Aceria tosichella (Keifer), is an important global pest of bread wheat, Triticum aestivum L. Chronic and often severe reductions of winter wheat yield due to A. tosichella infestations have occurred in North America and all other wheat-production areas for over five decades. Moreover, A. tosichella is the only vector which transmits the three most important wheat viruses in the Great Plains, which are Wheat Streak Mosaic Virus (WSMV), the most economically important wheat virus in North America; Triticum Mosaic Virus (TriMV) and High Plains Wheat Mosaic Virus (HPWMoV). Mite infestation alone causes stunted, chlorotic plants in susceptible wheat varieties. To date, mite resistant wheat cultivars have been the only sufficient method to control A. tosichella. The discovery of new genes for A. tosichella resistance and their introgression into wheat cultivars are essential steps to combat the development of new and/or different A. tosichella biotypes which can develop to overcome resistance genes. Both A. tosichella biotype 1 and 2 exist in U. S. Great Plains wheat producing areas. Elucidating and predicting A. tosichella population composition changes based on climatic and geographic variables is a key to continued effective mite management. Experiments were conducted to: 1) assess A. tosichella virulence in mites collected from 25 sample sites in six states to wheat plants harboring the Cmc2, Cmc3 and Cmc4 mite resistance genes and the Wsm2 WSMV resistance gene in 2014 and 2015, and determine the distribution of WSMV, TriMV and HPWMoV present in mites collected; 2) assess A. tosichella biotype composition using internal transcribed spacer 1 (ITS1) and cytochrome oxidase I (COI) polymorphisms; 3) use generalized additive modeling to capture the spatio-temporal factors contributing to the prevalence of A. tosichella biotypes 1 and 2; and 4) screen Kansas advanced breeding lines for resistance to A. tosichella biotypes 1 and 2. Results indicated that A. tosichella collected from 92% of the sample area were virulent to susceptible Jagger wheat plants with no Cmc resistance genes; that mites from 36% of the sample area were virulent to the Cmc2 gene, and that mites collected from 24% of sample area were virulent to Cmc3. Mite populations from only 8% of the sample sites exhibited virulence to plants containing Cmc4 + Wsm2 or Cmc4. The WSMV virus was predominant and present in 76% of all mites sampled. HPWMoV and TriMV were less apparent and present in 16% and 8% of all mites sampled, respectively. These results will enable breeders to increase the efficiency of wheat production by releasing wheat varieties containing A. tosichella resistance genes that contribute to reducing virus transmission. Results of spatio-temporal factor modeling provide new, more accurate information about the use of ground-cover and precipitation as key predictors of biotype prevalence and ratio. Experiments to determine if Kansas State University advanced breeding lines contain A. tosichella resistance found no resistance to biotype 1, resistance to biotype 2 in breeding lines AYN3-37 and AYN3-34; and moderate resistance to biotype 2 in breeding lines AYN2-28 and AYN2-36. The demonstrated correlation between reduced A. tosichella population size and avirulence; characterization and prediction of the A. tosichella biotype composition; and the identification of new sources of A. tosichella resistance in wheat can help entomologists and wheat breeders increase wheat production efficiency by releasing additional wheat cultivars containing A. tosichella resistance genes.

To improve management and control of jointed goatgrass (Aegilops cylindrica Host.) on traditional winter wheat (Triticum aestivum L.) cropland, a better understanding of the effects spring crop and wheat planting date have on weed populations and wheat yield is needed. A study of the effects of safflower (a spring crop) and wheat planting dates (early vs late) was conducted over a 2-yr period. Long term effects will be examined over a 5-yr period. The effects these treatments had on yield, weed seed contamination, jointed goatgrass population density, and soil seedbank concentration were measured. Two identical experiments were initiated, the first beginning in 1996, the second in 1997. In experiment one, initial plant counts showed similar numbers of jointed goatgrass plants in all treatments. In experiment two, initial spring plant counts showed increased numbers of jointed goatgrass in unplanted plots prior to planting safflower, and slightly reduced population densities in October-planted wheat when compared to September-planted wheat. Winter wheat yields were 25% and 35% higher in September-planted wheat than in October-planted wheat, in 1997 in experiment one, and1998 in experiment two, respectively. Crop contamination with jointed goatgrass propagules was four times higher in early vs late-planted wheat in 1997, and 36% higher in 1998. Jointed goatgrass plants in safflower were reduced 97% compared to preplan! counts in both experiments. In experiment one, 1998 fallow season plant counts showed 55% and 75% less jointed goatgrass in fallow safflower plots than in fallow plots of September- and October-planted wheat, respectively, with fallow plots of September-planted wheat having 46% less than fallow plots of October-planted wheat. Soil seed bank concentrations were highest at the 0-5 cm depth of October-planted wheat, which had nearly a 10-fold higher concentrations compared to safflower and September-planted wheat at this depth. There were no differences at depths below 5cm. This study showed the use of safflower to be a very useful management tool for reducing jointed goatgrass populations. September-planted wheat, with similar jointed goatgrass populations, yielded higher, and had less contamination and was therefore more competitive with jointed goatgrass than wheat planted in October, observed through a reduction in jointed goatgrass propagule production. Planting wheat in October, for the purpose of controlling jointed goatgrass through additional tillage, proved ineffective. Jointed goatgrass population densities were not reduced in experiment one, and only slightly reduced in experiment two. The dramatic loss of yield, associated with the later plantings, far outweighs any benefits gained by delaying wheat planting.

Soluble phosphorus fertilizer is bound in the soil rapidly after application in soils high in calcium. A fertilizer additive known as AVAIL® (J.R. Simplot Company) is purported to keep applied phosphorus fertilizer more available to plants by binding to soil minerals such as calcium, magnesium, and iron, thereby reducing phosphorus binding. This could prove useful due to the attraction of AVAIL® with cations such as Ca2+, but is fairly unstudied for dryland wheat production on alkaline, calcium-rich soils. The objective of this study is to evaluate the effect of low-rate fertilizer treatments with AVAIL® on dryland small grain yield on calcium-rich, eroded hillslopes in a fallow-wheat crop rotation. Two experiments were conducted to determine treatment effects on yield and grain quality for (1) above-ground dispersed (broadcast) application of monoammonium phosphate (MAP; 52% P2O5 content) fertilizer in the spring (2017), and (2) fall application of MAP incorporated with the seed (banded) at planting (2018). Fertilizer treatments were the recommended rate (60 lbs/ac) or one-half the recommended rate (30 lbs/ac) for dryland small grain, with or without AVAIL® (four treatments), replicated four times in a strip-block design for the 2017 experiment and replicated 3 times in a randomized complete block design for the 2018 experiment. Experimental blocks were assigned to hillslope erosional severity groups. The erosional severity groups were v designated (non-eroded, slightly eroded, highly eroded, and depositional slope segments). Hillslope segmentation allowed for correlations between calcium carbonate, organic matter, and yield levels across treatments. Results from the broadcast study indicate that there was no yield advantage of any treatment at any level of erosional severity, saving a grower $20.30/acre by applying 30 lbs/acre of MAP. However, 30 lbs/acre of MAP with AVAIL® showed similar yield to 60 lbs/acre of MAP without AVAIL®, potentially saving a grower $6.42/acre over standard growing practices. The incorporated study also indicated that there was no reliable yield advantage of any fertilizer treatment at any level of erosional severity, saving a grower $15.37/acre by applying 30 lbs/acre of MAP. Neither treatment with AVAIL had greater yield or profit than those without AVAIL. Profit for the 60 lbs/acre of MAP treatment narrowly outperformed 30 lbs/acre of MAP by $1.73/acre, indicating that growers may be able to reduce phosphorus use under dryland growing conditions with optimal fertilizer placement.

Orbital ATK tests horizontally restrained solid rocket motors (SRMs) in Promontory, Utah, USA. During SRMs tests, an estimated 1.5 million kg (~3.3 million lbs.) of entrained soil and combustion products (mainly aluminum oxide, gaseous hydrogen chloride, and water) are deposited over a wide area. Local farmers have expressed concerns regarding the deposition of this material, referred to as Test Fire Soil (TFS), on crops surrounding the test site. Greenhouse studies of mature corn and alfalfa (54 days to harvest) exposed to a worse case TFS deposition density to soil (3200-g/m2) resulted in reductions in germination and plant growth. Application of TFS only on leaves caused visual changes in leaf appearance (e.g. “burning”) but did not impact on growth. The visual damage to the leaves was thought to be caused by the high chloride in the TFS. The pH (measured from 2-10 in the field) of the TFS was also thought to be a potential cause of the visual damage. Follow-up studies were conducted using younger corn, alfalfa and winter wheat (12-54 days from emergence), to evaluate the impact of a more typical TFS deposition density and application scenario. The relationship between deposition amount and the crop impact was also examined. Impact was evaluated by comparing the growth, tissue composition (focusing on chloride and aluminum) and chlorophyll content of controls relative to the treatments. The individual effects of chloride and pH on corn leaves were determined by monitoring visual appearance and chlorophyll content. Leaves exposed to a typical TFS deposition loading (70-g/m2) did not contain elevated chloride or aluminum levels relative to the controls. Higher TFS loadings (700-g/m2 and 3200-g/m2) resulted in significantly elevated chloride content in corn leaves at 29 days from emergence. Corn leaves treated with TFS at the 700-g/m2 loading 12 days from emergence had comparable chloride levels to controls. High and low pH and chloride solutions visually impacted corn leaves and reduced leaf chlorophyll. This study indicated SRMs testing can be conducted under typical conditions during the juvenile growth stages of field corn, alfalfa, and winter wheat without adverse impact on crop yield and plant tissue composition.

Mycosphaerella graminicola (anamorph Septoria tritici) causes Septoria tritici blotch, a globally important disease of winter wheat. Resistance and pathogenicity generally vary quantitatively. The pathogen reproduces both sexually and asexually, and the pathogen population is highly genetically variable. Several unresolved questions about the epidemiology of this pathosystem are addressed by this research. Among them are whether cultivar-isolate specificity exists, how partial host resistance affects pathogen aggressiveness and sexual reproduction, and how host genotype mixtures influence epidemic progression and pathogenicity. At its release in 1992, the cultivar Gene was highly resistant to M. graminicola, but that resistance had substantially dissolved by 1995. Six of seven isolates collected in 1997 from field plots of Gene were virulent to Gene seedlings in the greenhouse, while 14 of 15 isolates collected from two other cultivars were avirulent to Gene. Gene apparently selected for strains of M. graminicola with specific virulence to it. In a two-year experiment, isolates were collected early and late in the growing season from field plots of three moderately resistant and three susceptible cultivars, and tested on seedlings of the same cultivars in the greenhouse. Isolates were also collected from plots of two susceptible cultivars sprayed with a fungicide to suppress epidemic development. Isolate populations were more aggressive when derived from moderately resistant than from susceptible cultivars, and more aggressive from fungicide-sprayed plots than from unsprayed plots of the same cultivars. Over 5,000 fruiting bodies were collected in three years from replicated field plots of eight cultivars with different levels of resistance. The fruiting bodies were identified as M. graminicola ascocarps or pycnidia, or other. In all three years, the frequency of ascocarps was positively correlated with cultivar susceptibility, as measured by area under the disease progress curve, and was also positively associated with epidemic intensity. For three years, four 1:1 mixtures of a moderately resistant and a susceptible wheat cultivar were planted in replicated field plots. Isolates from the plots were inoculated as bulked populations on greenhouse-grown seedlings of the same four cultivars. Mixture effects on disease progression varied among the years, and were moderately correlated with mixture effects on pathogenicity. / Graduation date: 2002

Graduation date: 1991

2005 - 2006 metais Lietuvos Žemės ūkio universiteto Bandymų stotyje, karbonatingame sekliai glėjiškame išplautžemyje (Calc(ar)i-Epihypogleyic Luvisols) IDg8-k (LVg-p-w-cc) atlikti tyrimai, siekiant įvertinti žieminių kviečių Titicum aestivum L. derlingumą ir kokybinių rodiklių pokyčius, naudojant skirtingas skystųjų kompleksinių trąšų KAS 32 ir Lyderis 25 - 3 S koncentracijas atskirais javų brendimo tarpsniais. Tyrimams pasirinkta žieminių kviečių veislė Lars, kuri priskiriama gerų kepimo savybių grupei. Nustatyta, kad papildomam žieminių kviečių tręšimui naudojant skystąsias kompleksines trąšas, patikimas derliaus priedas (1,64 – 2,14 t ha-1) gautas visuose variantuose, kuriuose tr��������šta N130 norma per augalų vegetaciją ir variante papildomai tręštame krūmijimosi tarpsnyje (N80) bei bambėjimo tarpsnyje (N20) norma. Žieminių kviečių papildomas tręšimas skystosiomis kompleksinėmis trąšomis gerino grūdų kokybę. Didžiausias kiekis šlapiojo glitimo ir baltymų nustatyta variantuose, kurie papildomai tręšti vėlyvaisiais augimo tarpsniais didesne N norma. / The productivity and quality traits of winter wheat (Triticum aestivum L.) under the influence of different liquidity fertilizers ‘KAS 32’ and ‘Lyderis 25-3S’ on special cereal ripeness were investigated at the Experimental Station of Lithuanian University of Agriculture in 2005-2006. The winter wheat variety ‘Lars’, which ascribable to well cooking group, were grown in the soil on trial fields – carbonate shallow gracious (Calc(ar)i-Epihypogleyic Luvisols) IDg8-k (LVg-p-w-cc). Our results showed, that using liquid manure for additional fertilization influenced significant yield additive (1.64-2.14 t ha-1) in all experiments, using N130 rate of fertilizers on plant growing season and additionally fertilized on tillering (N80) and stooling (N20) stages. Additional fertilization of winter wheat using liquid fertilizers improved grain quality. The greatest amount of wet gluten and protein were estimated in the experiments, using nitrogen at a premium rate for additional fartilization on late growth stages.

There are presented the following parts in the final master diploma work: introduction, 5 chapters, conslusions and list of literature. Total coverage of diploma work - 68 pages, including 59 pictures and 6 charts. There are presented sources or 24 information. The goal of diploma work - to range of the insert of the summer barley and winter wheat seeds with disc seeding maschine ploughshares in difeferently cultivated soils.

The simple effects of nitrogen rates, fungicide application, and varietal responses on wheat yield and quality have been studied individually, but interactions among the inputs are less well understood. Traditionally, increased nitrogen rates have led to higher risk of lodging and disease severity (Roth and Marshall, 1987), but it has been found that fungicides can be used to delay the onset of disease (Gooding et al. 1999). Similarly, yield responses to application of a fungicide may be affected by available nitrogen (Olesen et al., 2003). Wheat quality parameters such as protein, test weight, and alveograph parameters may also be affected by different management systems. Recent Ontario research shows interactions between fungicide and wheat varieties, even in the absence of disease, but the relationships are poorly understood. Current field crop recommendations would be improved by knowledge of interactions between inputs in more intensive management systems. Field experiments were established on nine farm fields in southwestern Ontario to investigate possible interactions among nitrogen rates, fungicides, and varieties. Eight fungicide timing combinations were applied as splits on three N rate blocks (100, 135, and 170 kg ha-1), applied across variety strips (between three and seven) in a split-strip-plot design with three replicates. Measurements included leaf disease severity, fusarium head blight incidence and severity and mycotoxin concentrations, grain yield and yield components, protein, test weight, and alveograph parameters. An economic analysis was performed on the combinations of treatment variables toward the development of more comprehensive recommendations. Interaction between N application rate and fungicide application resulted in greater yield gains with increased N when fungicides were used. Grain protein concentration increased at higher N application rates. Revenue net of the variable costs examined was not significantly affected by N rate alone, while effect of fungicide application varied with site. / Agricultural Adaptation Council, Grain Farmers of Ontario