Woody plant encroachment (WPE) into historic grasslands not only alters ecosystem structure but also yields a mosaic of vegetative growth-forms that differ in their inherent physiological capacities and physical attributes. C₃ plants tend to have a relatively broad range of temperature function but at the expensive of a lower optimum rate of photosynthesis. In contrast, C₄ grasses have a greater capacity for maximum uptake but across a relatively narrow range of temperatures. In considering which of these functional groups will outcompete the other within these regions undergoing WPE, one must account not only for these leaf physiological traits, but also the growth form induced differences in rooting depth, and therefore, potential access to deeper subsurface water. Laid upon these competitive interactions is an ever-changing environment, which for the semiarid southwestern US is predicted to become progressively warmer and characterized by highly variable precipitation with longer interstorm periods. In addition to aboveground changes in CO₂ assimilation, WPE influences soil nutrient, water, and carbon cycling. The objectives of this dissertation were to quantify: (1) the influence that temperature and available soil moisture have on regulating soil respiratory efflux within the microhabitats that results from WPE to estimate the influence this vegetative change will have on ecosystem CO₂ efflux; (2) the sensitivity of CO₂ uptake within grassland and woodland ecosystems to temperature and precipitation input in an effort to characterize how WPE might influence regional carbon and water balance; and (3) the role access to stable groundwater has in regulating the temperature sensitivity of ecosystems and their component fluxes. Major findings and contributions of this research include illustrating seasonal patterns of soil respiration within the microhabitats that result from WPE, such that an analysis of the relative contributions of these different components could be made. We found that soil respiration was not only consistently greater under mesquites, but that the relative contributions of these microhabitats varied significantly throughout the year, the duration of soil respiration after each rain was habitat-specific, and that the relationship between soil respiration and temperature followed a hysteretic pattern rather than a linear function (Appendix A). We found that a woodland ecosystem demonstrated a lower temperature sensitivity than a grassland across all seasonal periods of varying soil moisture availability, and that by maintaining physiological function across a wider range of temperatures throughout periods of limited precipitation, C₃ mesquites were acquiring large amounts of carbon while C₄ grasses were limited to functioning within a narrower range of temperatures (Appendix B). Finally, we found that having a connectivity to stable groundwater decoupled leaf and ecosystem scale temperature sensitivities relative to comparable sites lacking such access. Access to groundwater not only resulted in the temperature sensitivity of a riparian shrubland being nearly half that of the upland site throughout all seasonal periods, but also actual rates of net ecosystem productivity and leaf level rates of photosynthesis being dramatically enhanced (Appendix C).

The impact of undesirable plant communities (Cymbopogon afronardus and woody species dominated by Acacia species) on livestock carrying capacity and performance was investigated on 15 farms in an Acacia/Cymbopogon dominated pastoral system of south-western Uganda. Species prevalence based on basal cover for grasses, frequency for forbs and effective canopy cover for trees/shrubs were determined on farms. The PHYGROW model was used to predict forage productivity for computation of carrying capacity. The NIRS/NUTBAL nutritional management system was used to determine cattle dietary CP and DOM through fecal scans and to estimate animal performance. Cymbopogon afronardus had a prevalence of 10.29% among the grasses while Acacia gerrardii (34.37%) and Acacia hockii (33.66%) were the most prevalent woody species. Forage productivity differed significantly among the farms with a mean long-term annual forage yield of 4560(SE+41) kg/ha. Farms infested with Cymbopogon and woody species produced the least amount of forage and therefore had the lowest carrying capacities (0.38 -0.39 AU/ha) while improved farms had comparatively higher forage yields with higher carrying capacities (0.49 - 0.52 AU/ha). A mean carrying capacity for the system was estimated at 0.44 AU/ha using a 25% harvest efficiency for ANPP. All the farms were overstocked, on average by 3.2 times. Livestock BCS, diet CP and DOM were significantly different (P<0.0001) among the different farm types. BCS were highest on improved farms and lowest on Cymbopogon infested farms while dietary CP and DOM values were lowest on improved farms and highest on farms with a high woody component. Farms with a relatively high woody component exhibited intermediate BCS despite the high dietary CP values. Cattle on Cymbopogon infested farms had consistently lower body weights over the months although there were no significant differences in daily live weight gains among farms. Recommendations included need for research into appropriate control measures for both Cymbopogon afronardus and woody species, farmer sensitization about overstocking, research to improve forage quality on improved farms and need for feed supplementation for improved breeds on improved farms. Integration of NIRS/NUTBAL and PHYGROW models into the research and management systems was desirable. The observed increase in Sporobolus spp. required investigation.

Classical approaches to evaluate the stability of large woody debris (LWD) introduced in streams for habitat restoration or flood management purposes are usually based on inappropriate assumptions and hydraulic equations. Results suggest that the physics of small cylindrical elements located in large channels cannot be transferred to the case of a large roughness elements placed in small channels. The introduction of LWD in a small channel can generate a significant modification of the upstream hydraulic conditions. This modification has direct implications on the stability of the LWD. Experiments were performed in a controlled environment: a small stream section was represented by a low roughness rectangular flume and LWD were modeled with smooth PVC cylinders. Direct force measurements were performed with a load cell and results were used to identify an equation that evaluates the drag force acting on a large cylindrical element place in a rectangular channel. This equation does not depend on a drag coefficient. Water depths were also measured during the experiments and results were used to develop an approach that evaluates the upstream hydraulic impacts of a large cylinder introduced in a rectangular channel. The effect of the variation of the unit discharge (discharge per unit of width), cylinder size, cylinder elevation from the channel bed, and downstream hydraulic conditions, could be related to the upstream hydraulic conditions with relative success. Dimensionless parameters were developed to increase the versatility of the approach. The application of this approach to field cases is expected to require adjustments, mainly because of the roughness of natural environments differs from the smoothness of the controlled environment described in this work.

Saproxylic insects rely on dead or dying wood at some point in their development and appear to be sensitive to forest management. In 2005 and 2006, I sampled saproxylic insects in mixedwood boreal forests in northeastern Ontario to assess effects of: 1) different logging practices, 2) variations in woody debris (WD) qualities, and 3) different sampling methods. I also compared insect communities between heavily decayed WD and soil. Although I collected data on all arthropod orders, I focused on the families of the Diptera and parasitic Hymenoptera and the morphospecies of the Scelionidae and Diapriidae. These insects showed clear responses to the WD quality treatments, but not to the different logging treatments. A few families were affected by ex situ sampling. I also found that the faunas of soil and WD are rather distinct from each other. This study shows that WD is a source of forest biodiversity. Consequently, sustainable forest management will rely on studies like this to conserve and maintain the biodiversity of Canada's largest group of eco-zones.

Saproxylic insects rely on dead or dying wood at some point in their development and appear to be sensitive to forest management. In 2005 and 2006, I sampled saproxylic insects in mixedwood boreal forests in northeastern Ontario to assess effects of: 1) different logging practices, 2) variations in woody debris (WD) qualities, and 3) different sampling methods. I also compared insect communities between heavily decayed WD and soil. Although I collected data on all arthropod orders, I focused on the families of the Diptera and parasitic Hymenoptera and the morphospecies of the Scelionidae and Diapriidae. These insects showed clear responses to the WD quality treatments, but not to the different logging treatments. A few families were affected by ex situ sampling. I also found that the faunas of soil and WD are rather distinct from each other. This study shows that WD is a source of forest biodiversity. Consequently, sustainable forest management will rely on studies like this to conserve and maintain the biodiversity of Canada's largest group of eco-zones.

Ecosystem changes from grassland to shrubland in the Rio Grande Plains are thought to have negative effects on the hydrology of the region. The increase in woody plants, known as woody encroachment, may alter the amount of water moving beyond the root zone of plants. Water moving beyond the root zone is referred to as deep drainage, and has potential to become aquifer recharge. A vegetation manipulation project was designed to understand the effects of woody vegetation removal on soil water dynamics in the recharge zone of the Carrizo-Wilcox aquifer of south Texas. The primary objective of the project was to determine the potential to increase groundwater recharge through woody vegetation removal. To understand the effects of vegetation removal on various soil textures we studied changes in soil water, rooting depth, and the role of water redistribution by woody vegetation. Woody vegetation was removed using common methods of cut-stump and roller chop across three soil types. Soil water contents and changes were measured using neutron moisture meter to a depth of 180 cm. Average rooting depth was determined across three soil types. Soil and stem water stable isotopes were used to understand soil water movement. Rooting depth was determined to between 140 and 160 cm for all soil textures. Soil water content and changes were analyzed at three depth increments: 0-60, 60-120 and 120-180 cm. ANOVA analysis showed that there was no treatment response in average soil profile water in the sandy or sandy loam soils. There was a significant decrease in soil profile water for clay loam soil in response to roller chopping. Changes in soil profile water were the greatest in the sandy roller chopped soils. Below 120 cm, three months had significant differences in change in soil water in the sandy roller chop plot. During dry conditions, Honey mesquite shifts water use to deeper in the soil profile. In clay loam soils under dry conditions there is evidence of water being moved up from below 2 m soil depth to drier shallow soils. Roller chopping in sandy soils is the vegetation removal treatment and soil type most likely to result in water moving beyond the root zone. Although treatments had significant effects on soil moisture dynamics that interacted with soil type, we did not find support for deep drainage effects over the Carrizo-Wilcox aquifer from woody vegetation removal.

The encroachment of woody species into grassland and savanna ecosystems has been well document since the early 1800s. Within the parkland ecoregion of western Canada, trembling aspen (Populus tremuloides Michx.) has been one of the key tree species increasing in dominance. Aspen encroachment is best explained not by single mechanism, but rather by a number of interacting ecological factors. In this study I examined the ecological consequences of the clonal biology in aspen as a means to explain persistence and observed tree-grass ratios within grassland savannas of western Canada. Results suggest that aspen stands cycle between a “stable” phase characterized by a dense mature canopy, and an “unstable” phase characterized by canopy breakup and increased regeneration from root suckers. It is during this unstable phase that clonal encroachment is likely to occur. Within these mature stands, different-aged ramets promote persistence by maximizing developmental variation. Such a mechanism overcomes the functional phenotypic uniformity of ramets within a single age-structured stand. Results from my study indicate that physiological integration is beneficial to the growth and survivorship of regenerating and encroaching aspen ramets. My results also suggest that the benefits of physiological integration are greatest in more stressful environments, and in recently established post-fire ramets. Finally, the consequences of variation in adaptive ecological relevant traits among individuals was examined by determining differences in vigor among aspen clones in relation to the production of secondary compounds (phenolic glycosides). My results demonstrate a high degree of variation in leaf phenolic glycosides production among clones. A significant amount of this variation was accounted for by differences in clone vigor (within population: individual susceptibility hypothesis), with a smaller amount related to environmental differences (among populations). In all instances, vigorous clones were significantly higher in levels of phenolic glycosides compared with dieback clones, suggesting that some individuals may be predisposed to undergo density-independent mortality. This has important ecological implications, because it implies that one of the key mechanisms regulating population dynamics, community interactions and biodiversity may be related to intrinsic adaptive differences in susceptibility among individuals.

Verticillium wilt, caused by Verticillium dahliae Kleb, is one of the most economically important diseases of woody hosts such as ash (Fraxinus spp.), sugar maple (Acer saccharum), and redbud (Cercis canadensis). The causal agent has a broad host range, including not only woody hosts but also important vegetable and field crops, and it is distributed worldwide. Diagnosis of V. dahliae in infected woody hosts is often based on the occurrence of vascular discoloration and time-consuming isolation. However, not all woody hosts exhibit vascular discoloration symptoms, and not all vascular discoloration symptoms are due to infection by V. dahliae. In this study, real-time PCR-based assays were evaluated and employed for rapid and accurate detection of V. dahliae in different woody hosts. DNA was extracted in large quantities from presumptively infected woody hosts by collecting drill-press shavings from sample tissue, bead-beating, and extracting using a CTAB method. Six published primer sets were evaluated against genomic DNA of V. dahliae as well as selected negative controls, and two sets (VertBt-F/VertBt-R and VDS1/VDS2) showed promise for further evaluation using DNA extracts from field samples. The VertBt primers amplified a species-specific 115-bp fragment of the expected size, while the VDS primers amplified the expected specific 540-bp fragment. However, the VertBt primer set exhibited higher sensitivity in detection of V. dahliae even in asymptomatic trees. The PCR-based methods developed here could be used as rapid tools for pathogen detecting and monitoring, thus informing plant pathogen management decisions.

Several invasive woody plants grow in forest fragments at Muscatatuck National Wildlife Refuge in Seymour, Indiana. The objective of this study was to determine what woody species were growing in two upland forests and to assess the level of dominance that invasive species had in these areas. The variables of depth into the forest and directional aspect were also considered. Thirty-two belt transects were sampled and 54 woody species were found, including six invasive species: Ailanthus altissima (tree-ofheaven), Berberis thunbergii (Japanese barberry), Elaeagnus umbellata (autumn olive), Lonicera japonica (Japanese honeysuckle), Lonicera maackii (amur honeysuckle), and Rosa multiflora (multiflora rose). Importance values were calculated as an indicator of relative species dominance. Detrended Correspondence Analysis and Cluster analysis suggested that the invasive species were grouped on the forest edge transects; aspect was not found to be a determinant in where invasive species grew. Correlation coefficients demonstrated a significant difference between transect depth and the following variables at both forests: woody species density per transect, invasive species dominance, Elaeagnus umbellata density per hectare, and Lonicera japonica dominance. Rosa multiflora was one of the three most dominant understory species in both forests; on the edge transect, it was one of the top two. Soil temperatures were also measured at each transect, but no consistent trends were found within the data. In light of resources available, it is suggested that land managers focus on controlling Rosa multiflora and Ailanthus altissima. / Department of Natural Resources and Environmental Management

Šiame darbe aptariama šiaurės vakarų Lietuvoje, Plungės rajono savivaldybėje, esančio Babrungo upės baseino reguliuotose upėse vykstantys natūralizacijos procesai. Reguliuotose Babrungo ir Dirnupio upės pakrantės šlaituose ir apsaugos juostose aptikta 871 vienetai sumedėjusios augalijos. Tyrimais nustatyta, kad augalija dažniausiai ir tankiausiai auga šlaito viduriniojoje dalyje. Dirnupio upės tyrinėtame 1,8 km ruože sumedėjusios augalijos plotis kairiajame šlaite Lap = 2,11 ± 0,14 m, dešiniajame šlaite Lap = 1,71 ± 0,23 m. Babrungo upės tyrinėtame 2,2 km ruože apskaičiuotas kairiajame šlaite Lap = 1,86 ± 0,44 m, o dešiniajame šlaite Lap = 1,47 ± 0,27 m. Atlikus dispersinę analizę galima teigti, kad apaugusių sumedėjusia augalija šlaitų pločių vidurkiai, esant 95 % patikimumui, esminiai nesiskiria. Babrungo ir Dirnupio upės ruožų pakrančių apsaugos juostų pločiai miškuose ir pievose atitinka, o ariamoje žemėje neatitinka aplinkosauginių reikalavimų. Ariamose žemėse visi matuoti upės pakrantės apsaugos juostos pločiai buvo ≤5,0 m. / This article is about naturalization processes that take place in the regulated rivers of Babrungas river-basin in the northwestern Lithuania, Plungė district municipality. There are 871 units of woody vegetation in the riverside slopes and protective zones of the regulated Babrungas and Dirnupis rivers. Researchers have shown that usually the vegetation is the densest in the middle part of the slope. The width of woody vegetation on the left slope of 1.8 km strip of the Dirnupis river Lap = 2.11 ± 0.14 m, on the right slope Lap = 1.71 ± 0.23 m. On the left slope of 2.2 km strip of the Babrungas river Lap = 1.86 ± 0.44 m, and on the right slope Lap = 1.47 ± 0.27 m. The dispersive analysis allows to claim that given 95% of reliability, width averages of woody vegetation on the slopes do not differ. The widths of protective coast zones of Babrungas and Dirnupis rivers are the same in the woods and meadows, but in arable land they do not meet the environmental requirements. All widths of protective coast zones of arable land were ≥ 5.0 m.