Two outbreaks of salmonellosis were linked to the consumption of raw California almonds in 2001 and 2004. Current federal regulations mandate that all almonds grown in California are to be treated with a process that results in a 4-log reduction of Salmonella. Since four out of the five approved technologies to pasteurize almonds rely on the application of heat to control Salmonella, the evaluation of alternative technologies against heat resistant Salmonella Senftenberg was imminent. In this study, almonds that were inoculated with S. Enteritidis PT 30 and S. Senftenberg, were treated with electron beam irradiation (e-beam), blanching and oil roasting. The thermal death time (D-value) for S. Enteritidis PT 30 when treated with e-beam was 0.90 kGy, 15 s when subjected to blanching at 88 degrees C, and 13 s when treated with oil at 127 degrees C. Irradiation and thermal resistance of S. Senftenberg was not significantly different (P > 0.05) from S. Enteritidis PT 30. The commercial application of e-beam as a pathogen intervention was assessed through Monte Carlo simulations (MCS) and experimental measurements. The sensory characteristics of almonds commercially treated by e-beam, blanching and roasting were assessed by a consumer panel. Irradiated and blanched almonds did not differ in consumer overall like (P > 0.05). Bitterness and rancidity attributes of irradiated almonds were between a "dislike slightly" and "dislike moderately", whereas blanched and roasted almonds were between "neither like nor dislike" and "like slightly". Almonds commercially irradiated, blanched and roasted were subjected to an accelerated shelf-life test (ASLT) evaluating percentage free fatty acids, peroxide value, and 2-thiobarbituric acid reactive substances (TBARs). No clear differences between treatments were observed at any given point in time in any of the chemical tests. A gas chromatography-mass-spectrometry-olfactometry (MDGC-MS-O) technology was used to compare full aroma and flavor profiles from raw and e-beam irradiated almonds. Differences in the aroma/odor profile and the taste analysis revealed that the difference between raw and irradiated almonds is extremely subtle. In conclusion, e-beam may be a feasible technology to control Salmonella in almonds if used at low doses, as a part of a series of interventions.

After two outbreaks of salmonellosis that were linked to the consumption of almonds in 2001 and 2004, scientific community and industry have encouraged the study of pathogen inactivation kinetics in almonds. However, these studies often have overlooked the increase of water resulting from the inoculation of almonds. This increase of free water may result in data that may be overestimating the final outcome of treatments applied to pasteurize almonds in industrial settings. Therefore, in 2011 a study of almonds was performed at Texas A&M University to determine whether there was a need for Aw restoration. In this study, the effect of accelerated drying on the reduction of Salmonella on almonds was investigated, Salmonella Entritidis PT 30 isolated from one of the outbreaks linked to almonds, and Salmonella Typhimurium LT2 were used. For irradiation, the effect of vacuum packaging and air packaging on the sensitivity of Salmonella to e- beam irradiation was evaluated for both accelerated drying and conventional drying. The D value for Salmonella Entritidis PT 30 when treated with blanching at 88°C with accelerated drying was 10.7± 0.1 s, and 12.8 ± 0.3 s with conventional drying. When subjected to oil roasting at 127˚C, the D values were 10.5 ± 0.1 s with accelerated drying, and 10.2 ± 0.2 s with conventional drying. For irradiation treatments, the D10 value for vacuum packaged almonds was 0.35 ± 0.02 kGy with accelerated drying, and 0.38 ± 0.04 kGy with conventional drying. When irradiated in the presence of air, the D10 value of Salmonella was 0.26±0.04 kGy for almonds with accelerated drying, and 0.29 ± 0.03 kGy conventional drying. In conclusion, the accelerated drying process resulted in greater reduction in Salmonella on almonds in comparison to conventional drying when subjected to blanching but no differences were found for oil roasting (P > 0.05). For e-beam irradiation the D_10 values were significantly greater (P < 0.05) for vacuum with accelerated drying than for Air almonds with accelerated drying. This indicates that if a process applied in the industry were to be developed based on challenge studies when conventional drying was achieved, the almonds might likely be subjected to a treatment that is stronger than necessary to achieve pasteurization.

abstract: Nut consumption, specifically almonds, have been shown to help maintain weight and influence disease risk factors in adult populations. Limited studies have been conducted examining the effect of a small dose of almonds on energy intake and body weight. The objective of this study was to determine the influence of pre-meal almond consumption on energy intake and weight in overweight and obese adults. In this study included 21, overweight or obese, participants who were considered healthy or had a controlled disease state. This 8-week parallel arm study, participants were randomized to consume an isocaloric amount of almonds, (1 oz) serving, or two (2 oz) cheese stick serving, 30 minutes before the dinner meal, 5 times per week. Anthropometric measurements including weight, waist circumference, and body fat percentage were recorded at baseline, week 1, 4, and 8. Measurement of energy intake was self-reported for two consecutive days at week 1, 4 and 8 using the ASA24 automated dietary program. The energy intake after 8 weeks of almond consumption was not significantly different when compared to the control group (p=0.965). In addition, body weight was not significantly reduced after 8 weeks of the almond intervention (p=0.562). Other parameters measured in this 8-week trial did not differ between the intervention and the control group. These data presented are underpowered and therefore inconclusive on the effects that 1 oz of almonds, in the diet, 5 per week has on energy intake and bodyweight. / Dissertation/Thesis / M.S. Nutrition 2011

abstract: ABSTRACT Objective: The purpose of this randomized parallel two-arm trial was to examine the effect that an intervention of combining daily almond consumption (2.5 ounces) with a walking program would have on heart rate recovery and resting heart rate when compared to the control group that consumed a placebo (cookie butter) in men and postmenopausal women, aged 20-69, in Phoenix, Arizona. Design: 12 men and women from Phoenix, Arizona completed an 8-week walking study (step goal: 10,000 steps per day). Subjects were healthy yet sedentary, non-smokers, free from gluten or nut allergies, who had controlled blood pressure. At week 5, participants were randomized into one of two groups: ALM (2.5 oz of almonds daily for last 3 weeks of trial) or CON (4 tbsp of cookie butter daily for last 3 weeks of trial). Body weight, BMI, and percent body fat were measured using a stadiometer and Tanita at the screening visit. Resting heart rate, heart rate recovery, and anthropometric measurements were taken at weeks 0, 5, and 8. Results: 8 weeks of walking 10,000 steps per day, with or without 3 weeks of almond consumption did not significantly improve heart rate recovery (p=0.818) or resting heart rate (0.968). Conclusions: Almond consumption in combination with a walking intervention does not significantly improve heart rate recovery or resting heart rate. / Dissertation/Thesis / Masters Thesis Nutrition 2016

Peptídeos com ação inibitória sobre a enzima conversora de angiotensina I (ECA) e com o potencial de reduzir a pressão arterial têm sido obtidos a partir de diferentes tipos de alimentos ou matérias-primas, sendo grande o interesse em aproveitar resíduos da indústria alimentícia como fontes desses peptídeos. Neste aspecto, as amêndoas de cupuaçu (Theobroma grandiflorum S.), que são em sua maioria descartadas pela indústria, apresentam um teor considerável de proteínas e poderiam ser aproveitadas como fontes de peptídeos inibidores da ECA. Assim, o objetivo deste trabalho foi verificar se o concentrado proteico obtido a partir de amêndoas fermentadas de cupuaçu após ser submetido à hidrólise com a enzima pepsina poderia gerar peptídeos com ação inibitória sobre a ECA in vitro. Foi observado que após a hidrólise do concentrado proteico com pepsina por 1h foi obtido um efeito de 50 % de inibição da ECA, em ensaio realizado com o substrato Abz-FRK(Dnp)-P-OH. Posteriormente, esse hidrolisado foi submetido ao fracionamento por cromatografia em fase reversa (RP-HPLC) e resultou em cinco frações (F1-F5), das quais a terceira teve uma subfração (F3.1) com quatro novos peptídeos identificados por LC-MS/ MS com potencial em inibir a ECA. Esses quatro peptídeos (FWVAM, YRLAF, LGYFK, VTTVVTGLTF) foram sintetizados e submetidos aos ensaios para a determinação do IC50 e Ki. Os peptídeos YRLAF e LGYFK, que apresentaram mecanismo de inibição do tipo competitivo e acompetitivo, respectivamente, tiveram valores de IC50 de 4.73 e 11.11&#181;M, e de Ki de 9.14 e 8.15 &#181;M. Dentre os peptídeos identificados merece destaque VTTVVTGLTF que demonstrou ser um inibidor do tipo acompetitivo e apresentou as menores IC50 (0.70 &#181;M) e Ki (2.79 &#181;M). Em contraste, FWVAM atuou como substrato da ECA e não peptídeo inibidor. A partir dos resultados obtidos neste estudo fica demonstrado que as amêndoas fermentadas de cupuaçu podem ser fonte de peptídeos com ação inibitória da ECA, com potencial efeito anti-hipertensivo a ser, futuramente, investigado a partir de estudos in vivo. / Angiotensin converting enzyme (ACE) inhibitory peptides with the potential to reduce blood pressure have been obtained from different types of food or raw materials, and there is a great interest in utilize residues from the food industry as sources of peptides. In this regard, cupuassu almonds (Theobroma grandiflorum S.), which are mostly discarded by the industry, has a considerable protein content and could be used as source of ACE-inhibiting peptides. Thus, the objective of this work was to verify if the protein concentrate obtained from fermented almonds of cupuassu after being submitted to the hydrolysis with the enzyme pepsin could generate peptides with inhibitory activity on ACE in vitro. In this study it was observed that after hydrolysis of the protein concentrate with pepsin for 1 h, a 50% effect of ACE inhibition was obtained in an assay performed with the Abz-FRK (Dnp) -P-OH substrate. Posteriorly, the hydrolyzate was subjected to fractionation by reverse phase chromatography (RP-HPLC) and resulted in five fractions (F1-F5), of which the third had a subfraction (F3.1) with four new peptides identified by LC-MS / MS with the potential to inhibit ACE. These four peptides (FWVAM, YRLAF, LGYFK, VTTVVTGLTF) were synthesized and assayed for IC50 and Ki. The YRLAF and LGYFK peptides, which showed a competitive and uncompetitive type inhibition mechanism respectively, presented IC50 values of 4.73 and 11.11&#181;M, and the values for Ki were 9.14 and 8.15 &#181;M. Among the peptides identified, it is possible to highlight VTTVVTGLTF, which was shown to be an inhibitor of the uncompetitive type and presented the lowest value for IC50 (0.70 &#181;M) and Ki (2.79 &#181;M). While FWVAM acted as a substrate of the ACE and not as an inhibitory peptide. From the results obtained in this study it is demonstrated that cupuassu fermented almonds can be a source of peptides with ACE inhibitory activity with potential antihypertensive effect to be further investigated from in vivo studies.

A soils infiltration rate (IR) is the measured rate that soil is able to absorb water, either from precipitation or irrigation. A low IR can cause damage to crops if the necessary amount of water cannot penetrate to the plant roots in the time needed. The damage can be common in permanent plantings such as almond and pistachio orchards where regular tillage is avoided. This indicates a physical aspect to the problem because tillage increases IR. However, there is also an electrochemical side to infiltration problems because certain calcium surfactant treatments can increase IR. Various other methods have been used to increase IR such as using cover crops and increasing organic matter. Despite these different approaches to the problem, the specific cause of low IR is often unknown. This study was conducted to determine what physical properties of soils from a site in California cause low IR. This research shows the relationship of these properties to low IR. It was assumed that high amounts of mica in the very fine sand and coarse silt fraction of soils in Northwestern Kern County, California may interact with other physical and chemical properties to reduce IR. Fourteen sites were sampled in the area near the towns of Wasco and Shafter. The soils sampled represented the typical agricultural soils of the area and exhibited three levels of infiltration, Good (no amendments), Moderate (requires gypsum), and Poor (gypsum application is insufficient). Mica percentages in the very fine sand and coarse silt were calculated using a petrographic microscope and compared to the overall IR of the fields. Mica was shown to be significant factor at all three levels of IR.

Almonds are one of the first commercial nut trees to bloom in early spring and thus are susceptible to temperature patterns prior to and during bloom which affect bloom timing, bloom length, pollination and nut set. Data used in this project include yearly dates of 90% bloom from 1996-2006, bloom length in days and final crop yields in pounds per tree for Nonpareil and Mission varieties. Data were collected from the University of California Cooperative Extension reports on the 1993-2006 Regional Almond Variety Trials in Butte, San Joaquin and Kern Counties. Temperature pattern models in the form of Chill Hours (Chill Hour Model), Chill Units (Chill Unit Model), Chill Portions (Chill Portion Model) and Growing Degree Hours (GDH°) (Heat Model) prior to bloom were used to predict the date of 90% bloom for each variety, site and year. Temperature model results were compared to averaged actual dates of 90% bloom by site and variety used to predict bloom timing (Calendar Model). The relationship between bloom length in days and GDH° during bloom and the relationship between bloom length, GDH° during bloom and final crop yields were also evaluated. The average error in predicting the 90% bloom date for both Nonpareil and Mission was smaller using the Calendar Model compared to the four temperature pattern models. The Chill Portion model did not have significantly higher average error in predicting the date of 90% bloom than the Calendar model in Nonpareil. The Chill Unit and Chill Portion models had smaller errors in predicting 90% bloom date than the Chill Hour or GDH° model in Mission. GDH° during bloom was positively correlated with bloom length. GDH° during the first four days of Nonpareil bloom was significantly correlated with crop yields, with each additional GDH° during bloom correlated with a 0.4 lbs./tree increase in crop yield. Further research is needed on specific temperature thresholds and their relationship to physiological changes during almond bloom and pollination. The practice of monitoring chilling and heat accumulation will allow growers to anticipate bloom, prepare to optimize bee activity during bloom, and plan for possible crop yield variations due to adverse weather conditions during bloom in almonds.

CARVALHO, Michelle Garcêz de. Barras de cereais com amnêndoas de chichá, sapucaia e castanha-do-gurguéia, complementadas com casca de abacaxi. 2008. 92 f. : Dissertação (mestrado) - Universidade Federal do Ceará, Centro de Ciências Agrárias, Departamento de Tecnologia de Alimentos, Fortaleza-CE, 2008 / Submitted by Nádja Goes (nmoraissoares@gmail.com) on 2016-06-21T13:50:58Z No. of bitstreams: 1 2008_2008_mgcarvalho.pdf: 539660 bytes, checksum: 6fd4106e3146dfc07ee4afb629725644 (MD5) / Approved for entry into archive by Nádja Goes (nmoraissoares@gmail.com) on 2016-06-21T13:51:36Z (GMT) No. of bitstreams: 1 2008_2008_mgcarvalho.pdf: 539660 bytes, checksum: 6fd4106e3146dfc07ee4afb629725644 (MD5) / Made available in DSpace on 2016-06-21T13:51:36Z (GMT). No. of bitstreams: 1 2008_2008_mgcarvalho.pdf: 539660 bytes, checksum: 6fd4106e3146dfc07ee4afb629725644 (MD5) Previous issue date: 2008 / The trend of consumption of healthy food, innovative and practical has led to growth increasing in recent years the market for the cereal bars. The cereal bars are formed by grain cereals, processed and clusters, and they can be incorporated different ingredients, such as integral cereals, dehydrated fruit or crystallized fruit, almonds and sugars. There are in the native Brazilian flora, some species still little known, but which have potential for the market of almonds, as chichá, sapucaia and chestnut-of-gurguéia. However, its observe also the importance of the use of waste generated from fruit processing, such as bark, stems and cake, these can be used in the human diet. Thus, the objective of this work was to develop the cereal bars from almonds to chichá, sapucaia and chestnut-of-gurguéia, supplemented with pineapple bark. Cereal bars were making with three formulations (A, B and C), which they were different by the amount added of hydrogenated vegetable fat and dust of the pineapple bark. Subsequently, nine types of the cereal bars were prepared, three with almonds, chichá, three with almonds, chestnut-do-gurguéia and three of sapucaia with almonds, which were evaluated microbiologically and sensorial. After the sensory evaluation, the cereal bars with higher notes of overall impression was chose, and those subjected to chemical analyses. The sapucaia highlighted is among the almonds evaluated, by presenting the greatest medium for most parameters evaluated. It was found by the parameter overall impression that among the formulations tested showed that the largest note of acceptance was the formulation B, between the cereal bars with the three almonds studied. The cereal bars, developed with almonds of chichá and sapucaia, in general, were more accepted than cereal bars with almonds of chestnut-do-gurguéia. With regard to minerals, the highest levels in the cereal bars prepared in this study were, respectively, potassium, sodium, iron and zinc. It is viable to use the almonds, chichá, sapucaia and chestnut-of-gurguéia, and the dust of the pineapple bark in the preparation of the cereal bars. / A tendência de consumo de alimentos saudáveis, inovadores e práticos tem levado ao crescimento cada vez maior nos últimos anos do mercado de barras de cereais. As barras de cereais são formadas por grãos de cereais processados e aglomerados, e a elas podem ser incorporados diferentes ingredientes, tais como cereais integrais, frutas desidratadas ou cristalizadas, amêndoas e açúcares. Existem na flora nativa brasileira, algumas espécies ainda pouco conhecidas, mas que apresentam potencialidades para o mercado de amêndoas, como o chichá, sapucaia e castanha-do-gurguéia. No entanto, observa-se também a importância da utilização dos resíduos gerados do processamento das frutas, como cascas, talos e bagaços, podem esses ser aproveitados na dieta humana. Assim, objetivou-se neste trabalho desenvolver barras de cereais a partir de amêndoas de chichá, sapucaia e castanha-do-gurguéia, complementadas com casca de abacaxi. Foram formuladas barras de cereais com três formulações (A, B e C), as quais se diferenciavam pela quantidade adicionada de gordura vegetal hidrogenada e pó da casca de abacaxi. Posteriormente, foram elaborados nove tipos de barras de cereais, sendo três com amêndoas de chichá, três com amêndoas de castanha-do-gurguéia e três com amêndoas de sapucaia, as quais foram avaliadas microbiologicamente e sensorialmente. Após a avaliação sensorial, escolheram-se as barras de cereais com maiores notas de impressão global, sendo essas submetidas a análises químicas. A sapucaia destacou-se, dentre as amêndoas avaliadas, por apresentar as maiores médias para a maioria dos parâmetros avaliados. Verificou-se através do parâmetro impressão global que dentre as formulações testadas a que exibiu a maior nota de aceitação foi a formulação B, entre as barras de cereais com as três amêndoas estudadas. As barras de cereais elaboradas com amêndoas de chichá e sapucaia de um modo geral foram mais aceitas do que as barras de cereais com amêndoas de castanha-do-gurguéia. No que se referem aos minerais, os de maior teor nas barras de cereais elaboradas neste estudo, foram, respectivamente, o potássio, sódio, ferro e zinco. Conclui-se ser viável a utilização das amêndoas de chichá, sapucaia e castanha-do-gurguéia, e do pó da casca de abacaxi na elaboração de barras de cereais.

A tendÃncia de consumo de alimentos saudÃveis, inovadores e prÃticos tem levado ao crescimento cada vez maior nos Ãltimos anos do mercado de barras de cereais. As barras de cereais sÃo formadas por grÃos de cereais processados e aglomerados, e a elas podem ser incorporados diferentes ingredientes, tais como cereais integrais, frutas desidratadas ou cristalizadas, amÃndoas e aÃÃcares. Existem na flora nativa brasileira, algumas espÃcies ainda pouco conhecidas, mas que apresentam potencialidades para o mercado de amÃndoas, como o chichÃ, sapucaia e castanha-do-gurguÃia. No entanto, observa-se tambÃm a importÃncia da utilizaÃÃo dos resÃduos gerados do processamento das frutas, como cascas, talos e bagaÃos, podem esses ser aproveitados na dieta humana. Assim, objetivou-se neste trabalho desenvolver barras de cereais a partir de amÃndoas de chichÃ, sapucaia e castanha-do-gurguÃia, complementadas com casca de abacaxi. Foram formuladas barras de cereais com trÃs formulaÃÃes (A, B e C), as quais se diferenciavam pela quantidade adicionada de gordura vegetal hidrogenada e pà da casca de abacaxi. Posteriormente, foram elaborados nove tipos de barras de cereais, sendo trÃs com amÃndoas de chichÃ, trÃs com amÃndoas de castanha-do-gurguÃia e trÃs com amÃndoas de sapucaia, as quais foram avaliadas microbiologicamente e sensorialmente. ApÃs a avaliaÃÃo sensorial, escolheram-se as barras de cereais com maiores notas de impressÃo global, sendo essas submetidas a anÃlises quÃmicas. A sapucaia destacou-se, dentre as amÃndoas avaliadas, por apresentar as maiores mÃdias para a maioria dos parÃmetros avaliados. Verificou-se atravÃs do parÃmetro impressÃo global que dentre as formulaÃÃes testadas a que exibiu a maior nota de aceitaÃÃo foi a formulaÃÃo B, entre as barras de cereais com as trÃs amÃndoas estudadas. As barras de cereais elaboradas com amÃndoas de chichà e sapucaia de um modo geral foram mais aceitas do que as barras de cereais com amÃndoas de castanha-do-gurguÃia. No que se referem aos minerais, os de maior teor nas barras de cereais elaboradas neste estudo, foram, respectivamente, o potÃssio, sÃdio, ferro e zinco. Conclui-se ser viÃvel a utilizaÃÃo das amÃndoas de chichÃ, sapucaia e castanha-do-gurguÃia, e do pà da casca de abacaxi na elaboraÃÃo de barras de cereais. / The trend of consumption of healthy food, innovative and practical has led to growth increasing in recent years the market for the cereal bars. The cereal bars are formed by grain cereals, processed and clusters, and they can be incorporated different ingredients, such as integral cereals, dehydrated fruit or crystallized fruit, almonds and sugars. There are in the native Brazilian flora, some species still little known, but which have potential for the market of almonds, as chichÃ, sapucaia and chestnut-of-gurguÃia. However, its observe also the importance of the use of waste generated from fruit processing, such as bark, stems and cake, these can be used in the human diet. Thus, the objective of this work was to develop the cereal bars from almonds to chichÃ, sapucaia and chestnut-of-gurguÃia, supplemented with pineapple bark. Cereal bars were making with three formulations (A, B and C), which they were different by the amount added of hydrogenated vegetable fat and dust of the pineapple bark. Subsequently, nine types of the cereal bars were prepared, three with almonds, chichÃ, three with almonds, chestnut-do-gurguÃia and three of sapucaia with almonds, which were evaluated microbiologically and sensorial. After the sensory evaluation, the cereal bars with higher notes of overall impression was chose, and those subjected to chemical analyses. The sapucaia highlighted is among the almonds evaluated, by presenting the greatest medium for most parameters evaluated. It was found by the parameter overall impression that among the formulations tested showed that the largest note of acceptance was the formulation B, between the cereal bars with the three almonds studied. The cereal bars, developed with almonds of chichà and sapucaia, in general, were more accepted than cereal bars with almonds of chestnut-do-gurguÃia. With regard to minerals, the highest levels in the cereal bars prepared in this study were, respectively, potassium, sodium, iron and zinc. It is viable to use the almonds, chichÃ, sapucaia and chestnut-of-gurguÃia, and the dust of the pineapple bark in the preparation of the cereal bars.

Peptídeos com ação inibitória sobre a enzima conversora de angiotensina I (ECA) e com o potencial de reduzir a pressão arterial têm sido obtidos a partir de diferentes tipos de alimentos ou matérias-primas, sendo grande o interesse em aproveitar resíduos da indústria alimentícia como fontes desses peptídeos. Neste aspecto, as amêndoas de cupuaçu (Theobroma grandiflorum S.), que são em sua maioria descartadas pela indústria, apresentam um teor considerável de proteínas e poderiam ser aproveitadas como fontes de peptídeos inibidores da ECA. Assim, o objetivo deste trabalho foi verificar se o concentrado proteico obtido a partir de amêndoas fermentadas de cupuaçu após ser submetido à hidrólise com a enzima pepsina poderia gerar peptídeos com ação inibitória sobre a ECA in vitro. Foi observado que após a hidrólise do concentrado proteico com pepsina por 1h foi obtido um efeito de 50 % de inibição da ECA, em ensaio realizado com o substrato Abz-FRK(Dnp)-P-OH. Posteriormente, esse hidrolisado foi submetido ao fracionamento por cromatografia em fase reversa (RP-HPLC) e resultou em cinco frações (F1-F5), das quais a terceira teve uma subfração (F3.1) com quatro novos peptídeos identificados por LC-MS/ MS com potencial em inibir a ECA. Esses quatro peptídeos (FWVAM, YRLAF, LGYFK, VTTVVTGLTF) foram sintetizados e submetidos aos ensaios para a determinação do IC50 e Ki. Os peptídeos YRLAF e LGYFK, que apresentaram mecanismo de inibição do tipo competitivo e acompetitivo, respectivamente, tiveram valores de IC50 de 4.73 e 11.11&#181;M, e de Ki de 9.14 e 8.15 &#181;M. Dentre os peptídeos identificados merece destaque VTTVVTGLTF que demonstrou ser um inibidor do tipo acompetitivo e apresentou as menores IC50 (0.70 &#181;M) e Ki (2.79 &#181;M). Em contraste, FWVAM atuou como substrato da ECA e não peptídeo inibidor. A partir dos resultados obtidos neste estudo fica demonstrado que as amêndoas fermentadas de cupuaçu podem ser fonte de peptídeos com ação inibitória da ECA, com potencial efeito anti-hipertensivo a ser, futuramente, investigado a partir de estudos in vivo. / Angiotensin converting enzyme (ACE) inhibitory peptides with the potential to reduce blood pressure have been obtained from different types of food or raw materials, and there is a great interest in utilize residues from the food industry as sources of peptides. In this regard, cupuassu almonds (Theobroma grandiflorum S.), which are mostly discarded by the industry, has a considerable protein content and could be used as source of ACE-inhibiting peptides. Thus, the objective of this work was to verify if the protein concentrate obtained from fermented almonds of cupuassu after being submitted to the hydrolysis with the enzyme pepsin could generate peptides with inhibitory activity on ACE in vitro. In this study it was observed that after hydrolysis of the protein concentrate with pepsin for 1 h, a 50% effect of ACE inhibition was obtained in an assay performed with the Abz-FRK (Dnp) -P-OH substrate. Posteriorly, the hydrolyzate was subjected to fractionation by reverse phase chromatography (RP-HPLC) and resulted in five fractions (F1-F5), of which the third had a subfraction (F3.1) with four new peptides identified by LC-MS / MS with the potential to inhibit ACE. These four peptides (FWVAM, YRLAF, LGYFK, VTTVVTGLTF) were synthesized and assayed for IC50 and Ki. The YRLAF and LGYFK peptides, which showed a competitive and uncompetitive type inhibition mechanism respectively, presented IC50 values of 4.73 and 11.11&#181;M, and the values for Ki were 9.14 and 8.15 &#181;M. Among the peptides identified, it is possible to highlight VTTVVTGLTF, which was shown to be an inhibitor of the uncompetitive type and presented the lowest value for IC50 (0.70 &#181;M) and Ki (2.79 &#181;M). While FWVAM acted as a substrate of the ACE and not as an inhibitory peptide. From the results obtained in this study it is demonstrated that cupuassu fermented almonds can be a source of peptides with ACE inhibitory activity with potential antihypertensive effect to be further investigated from in vivo studies.