The mare experiences a transient innate immune response to breeding, the resolution of which is crucial for optimal fertility. The majority of mares are able to modulate this inflammation in a timely fashion, but a subpopulation exists which fail to do so and are considered susceptible to persistent breeding-induced endometritis (PBIE). Seminal plasma has been shown to modulate aspects of this inflammation. Recently, two seminal plasma proteins have garnered interest for their immune modulating properties: cysteine-rich secretory protein-3 (CRISP-3) and lactoferrin. These proteins have been found to alter the binding between sperm and neutrophils based on sperm viability in vitro, but minimal work has evaluated their effect on endometrial mRNA expression of cytokines and inflammation in response to breeding. Experiments were performed to analyze the expression of equine CRISP-3. Found to be primarily synthesized in the ampulla of the vas deferens and to a lesser extent in the vesicular gland, CRISP-3 expression was only seen in the postpubertal stallion. Due to the effect of sperm viability on protein function in vitro, varying sperm populations were analyzed for their effect on gene expression in the uterus. It was determined that viable sperm suppressed the gene expression of the inflammatory modulating cytokine interleukin-6 (IL-6) in comparison to dead sperm. Next, the effect of CRISP-3 and lactoferrin on endometrial gene expression in the normal and susceptible mare was investigated. Neither protein had a significant effect on the mRNA expression of inflammatory cytokines in the normal mares at six hours post-breeding. In contrast, lactoferrin was found to significantly suppress the expression of the pro-inflammatory cytokine tumor necrosis factor (TNF)-α in susceptible mares. Due to this, lactoferrin was further analyzed as an immunomodulant for the treatment of PBIE. Susceptible mares were infused with varying doses of lactoferrin at six hours post-breeding. Although not in a dose-dependent fashion, lactoferrin was found to decrease both fluid retention and neutrophil migration, in addition to suppressing the expression of the pro-inflammatory cytokine interferon gamma (IFNγ) and increasing the gene expression of the anti-inflammatory cytokine interleukin-1 receptor antagonist (IL-1RN). In conclusion, CRISP-3 expression occurs in secretory aspects of the male reproductive tract, and appears to be up regulated after sexual maturation. Viability of spermatozoa affects the immune response to breeding and should be taken into consideration for experimental design and interpretation of data. The seminal plasma proteins CRISP-3 and lactoferrin have minimal effect on endometrial gene expression in normal mares, but lactoferrin suppresses the expression of TNF in susceptible mares. Finally, lactoferrin was found to function as a potent anti-inflammatory for the persistent inflammation seen in susceptible mares when administered post-breeding. This protein should be further investigated as a potential therapeutic for the treatment of persistent breeding-induced endometritis.

Objetivo: Alternativas para o tratamento da doença periodontal necessitam ser testadas e uma opção viável parece ser o uso da terapia antimicrobiana fotodinâmica. O objetivo deste estudo foi investigar o efeito da terapia antimicrobiana fotodinâmica através das alterações no perfil microbiológico e no padrão de citocinas após o tratamento da doença periodontal induzida por ligaduras em cães. Métodos: Para tanto, foi induzida doença periodontal através da colocação de ligaduras em torno dos pré-molares mandibulares bilaterais em oito cães. Após a indução da doença os sítios foram aleatoriamente tratados através de raspagem e alisamento radicular (RAR) com instrumentos manuais, terapia antimicrobiana fotodinâmica (TAF) com a utilização de uma fonte de luz laser associada a um fotosensitizador, ou através da combinação das terapias. Para a análise da resposta dos sítios frente aos tratamentos, diferentes tempos experimentais foram utilizados (baseline, uma, três e quatro semanas) onde foram coletadas amostras de placa dentobacteriana subgengival e a contagem de 40 espécies microbianas foi determinada através da técnica de hibridização DNA-DNA. Além disso, amostras de tecidos periodontais foram removidas e a expressão gênica de diferentes citocinas (TNF-&alpha;, RANKL, OPG, MMP-1, IL-6 e IL-10) foi determinada pela técnica de reação em cadeia de polimerase quantitativa em tempo real. Os dados foram analisados através dos testes de equações de estimação generalizadas (EEG) e análise da variância (ANOVA) com um nível de significância de 0,05. Resultados: Os dados demonstraram uma redução dos níveis da maioria das espécies bacterianas monitoradas após uma semana para todos os tratamentos realizados (P < 0,001), no entanto, um aumento na contagem média de P. intermedia (P <0,01), P. nigrescens (P < 0,001) e T. forsythia (P < 0,05) foi observado para os sítios tratados por TAF e por RAR + TAF. Após quatro semanas foi observada uma possível recolonização de P. gingivalis e T. denticola, para todos os tratamentos testados (P < 0,001). A contagem média de T. forsythia manteve-se elevada durante o curso do estudo, especialmente para TAF e RAR + TAF. Adicionalmente foi observada uma notável redução da contagem média de A. actinomycetemcomitans para o grupo TAF (P < 0,001). Em relação ao perfil das citocinas avaliadas, os resultados foram similares para todos os tratamentos testados. O fator tempo foi considerado estatisticamente significante independente do tratamento realizado (P < 0,001) e não foi observada influência dos tratamentos sobre os resultados (P > 0,05). Foi observada uma redução progressiva na expressão das citocinas durante os períodos de observação, entretanto entre os períodos de três e quatro semanas não houve diferença estatisticamente significante (P > 0,05). Conclusões: De acordo com o modelo experimental utilizado neste estudo os dados sugerem que os tratamentos testados afetaram diferentemente as espécies bacterianas monitoradas e apresentaram um efeito similar na expressão das citocinas avaliadas. / The purpose of this study was to evaluate the effect of a single application of antimicrobial photodynamic therapy (aPDT) on microbiological profile and cytokine pattern in dogs. Periodontal disease was induced by placing 3.0 silk ligatures around the mandibular pre-molars bilaterally during 8 weeks. The dogs were randomly treated with aPDT using a dye/laser system, scaling and root planning (SRP) or with the association of treatments (SRP+aPDT). Plaque samples were collected at baseline, 1, 3 and 4 weeks and the mean counts of 40 species were determined using DNA-DNA hybridization. Gingival biopsies were removed and the expression of tumor necrosis factor alpha (TNF-&alpha;), receptor activator of NF-kB ligand (RANKL), osteoprotegerin (OPG), matrix metalloproteinase (MMP-1), interleukin (IL) 6, IL-10 and total bacterial load by analysis of 16S rRNA gene were evaluated through RealTime PCR. The results shows that the levels of the majority of the species were reduced one week post therapy for all treatments, however, an increase in counts of Prevotella intermedia (P =0.00), Prevotella nigrescens (P =0.00) and Tannerella forsythia (P =0.00) was observed for aPDT and SRP+aPDT. After 4 weeks a regrowth of Porphyromonas gingivalis (P =0.00) and Treponema denticola (P =0.00), was observed for all treatments, also a strikingly reduction of counts on counts of Aggregatibacter actinomycetemcomitans was observed for the aPDT (P =0.00). For the cytokine pattern the results were similar for all treatments and it was observed a reduction in the expression of cytokines and bacterial load throughout the study. Our results suggest that SRP, aPDT in a single application and SRP+aPDT affects different bacterial species and have similar effects on the expression of cytokines evaluated during the treatment of ligature-induced periodontitis.

Fantasy creatures are an essential part of many games, but while there are several studies focusing on body language and how expressive gaming companions may enhance the player’s experience, creatures and animals are rarely the focal points. Personality is closely related to believability, which is what most game developers work towards hence believability may improve the gaming experience. The purpose of this paper was to explore how the personality of a fantasy creature would be perceived by the observer when using different animal motion references. A 3D-model was created and animated in three different styles using motion references from a cat and lizard. A survey with Likert-scales was then formed with the intention to evaluate the animations. The participants in the survey were assigned one of the three animations to rate statements regarding personality and believability. Rather than a certain type of animal being associated with a certain type of personality, the result suggests that it was mainly certain motion cues and postures that affected the participants’ ratings. The study was deemed to be insufficient for a reliable result. In the discussion part, there are ideas on how the study could be improved with the aim of continuing the research to gain a clearer insight into the subject of personality and creatures.

The endangered white-cheeked crested gibbon (Nomascus leucogenys), native to Laos, Vietnam, and perhaps China, remains little known and highly threatened. I studied seasonal variation in the diet, activity budget, and ranging behavior of three groups of white-cheeked crested gibbons in Nam Kading National Protected Area, Bolikhamxay Province, Laos, over 12 months in wet seasonal evergreen forest. Crested gibbons (Nomascus spp.) are speculated to be more folivorous than other gibbons, but this has never been confirmed because of the paucity of fieldwork on the genus. I studied diet in relation to forest seasonality to determine the contribution of leaves to the diet over an annual cycle. Although leaves were the main dietary item throughout the year (53-85% of monthly diet), gibbons substantially increased their consumption of fruit during periods when it was most abundant in the forest. Because fruit is a calorically rich source of food, gibbons seek fruit when it is abundant and obtained easily. Young leaf consumption increased when they did not have access to fruit, indicating that their diet is flexible but strongly dependent on seasonal availability of resources. In addition, rainfall had a negative association with fruit abundance and fruit in diet. Activity budgets are an important aspect of a species' ecology because they are directly related to home range use, energy allocation, and diet, but they have never before been studied in gibbons (Nomascus spp.) of the rainy, mountainous, forests of Laos. Annually, the three groups that I studied spent nearly equal amounts of time resting (30%), feeding (33%), and traveling (35%), but only a small amount of time singing (2%). However, the proportion of time allocated to different activities showed significant seasonal variation associated with rainfall and diet, and correlated with home range use. Gibbons increased traveling time and decreased feeding time when they ate more fruit, and they decreased traveling time and increased feeding time when they ate more leaves. When the gibbons spent more of their time traveling, they also had longer day range lengths, and used a higher percentage of their total home range. Moreover, when rainfall was high, the gibbons decreased traveling time and increased time resting and feeding. Average home range size was 37.9 hectares and daily average distance over which the gibbons ranged over the 12 month study was 1.48 km per day. Differences existed among the three groups. Overall, white-cheeked crested gibbons have a home range similar in size or larger than frugivorous gibbons, and larger than the folivorous siamang. Ranging was highly seasonal with shorter day ranges during times of low fruit availability and consumption. During times of high fruit availability and low rainfall, the gibbons took on an energy maximizing strategy where they maintained large home ranges, traveled longer distances and consumed larger quantities of fruit. Gibbons and their habitat in Laos have faced continuous threats over the past 10 years because of large scale development projects and subsistence hunting. Given the highly threatened status of the species in Vietnam and China, the Lao population is certainly the world's largest and the best hope for conservation of behavioral, ecological, and genetic diversity. Resources for conserving species in the country are very limited, and wildlife populations are already greatly fragmented. I describe the current conservation issues and based on the dietary and ranging information that I collected, recommend important conservation measures to safeguard the remaining populations of endangered gibbons from extinction.

In this dissertation I analyze how the postwar German writers Wolfdietrich Schnurre, Günter Eich, and Ilse Aichinger negotiate anthropocentric and speciesist discourses via animal figures by drawing on such posthumanist thinkers as Derrida, Agamben, and Deleuze & Guattari. The literary texts question a world view and discourse organized around the establishment of power that utilizes animal metaphors to turn living beings into objects (and could thus be called “carno-phallogocentric”). They thus react to the strict hierarchy of (gendered) man over animal and respond - in the aftermath of the Second World War – by highlighting instead the similarities between man and animal, such as creaturely existence and shared trauma. The analysis is guided by questions such as: How do the literary texts reflect and subvert the power discourses which surround man and animal? What is the role of language in this context? How does the animal, which is usually assumed to be mute, relate to the categories that are established in language? Does its place outside of language grant it capabilities the human cannot realize? Can the literary encounter between man and animal establish a space of the “Open” in which language can be re-evaluated and, after World War II, be saved? Is there a unique “animal poetology” which correlates to post-anthropocentric conceptions of the human? Because these writers disorient the reader’s perception of reality via figures of the animal, i.e., animals as both metaphors and as subjects, I develop what I would like to call an “animal poetology” that is unique to them. This animal poetology, which redefines Agamben’s concept of the open by giving it a postwar, language-critical dimension, includes a thorough critique of human language with regard to power structures and a speciesist language which, during the early 20th century, was a vehicle for ideology and discrimination. The encounter with the animal leads the human being to reflect on the limits of language and thus enables the establishment of a mode of being in which the encounter with the other – beyond a space of judgement and hierarchies –is once again possible.

The Ambivalent Animal project explores the interactions of animals, culture and technology. The project employs both artistic practice and critical theory, each in ways that inspire the other. My creative practice centers around two projects that focus on domestic pets. These projects highlight the animal's uncertain status as they explore the overlapping ontologies of animal, human and machine. They provide concrete artifacts that engage with theoretical issues of anthropocentrism, animality and alterity. My theoretical work navigates between the fields of animal studies, art and design, media and culture studies, and philosophy. My dissertation explores animality through four real and imagined animal roles: cyborg, clone, chimera and shapeshifter. Each animal role is considered in relation to three dialectics: irreducibility and procedurality, autonomy and integration, aura and abjection. These dialectics do not seek full synthesis but instead embrace the oscillations of irresolvable debates and desires. The dialectics bring into focus issues of epistemology, ontology, corporeality and subjectivity. When the four animal roles engage the three dialectics, connected yet varied themes emerge. The cyborgian animal is simultaneously liberated and regulated, assisted and restricted, integrated and isolated. The cloned animal is an emblem of renewal and loss; she is both idealized code and material flesh and finds herself caught in the battles of nature and nurture. The chimera is both rebel and conformist; his unusual juxtapositions pioneer radical corporeal transgressions but also conform to the mechanisms of global capital. And the shapeshifter explores the thrill and anxiety of an altered phenomenology; she gains new perceptions though unstable subjectivity. These roles reveal corporeal adjustments and unfamiliar subjectivities that inspire the creative practice. Both my writing and making employ an ambivalent aesthetic--an aesthetic approach that evokes two or more incompatible sensibilities. The animal's uncertain status contributes to this aesthetic: some animals enjoy remarkable care and attention, while others are routinely exploited, abused and discarded. Ambivalence acknowledges the complexity of lived experience, philosophical and political debate, and academic inquiry. My approach recognizes the light and dark of these complex ambivalences--it privileges paradox and embraces the confusion and wonder of creative research. Rather than erase, conceal or resolve ambiguity, an ambivalent aesthetic foregrounds the limits of language and representation and highlights contradiction and irresolution.

I Sociologiskt perspektiv på Hästunderstödd terapi studeras hur deltagares upplevelser och effekter av Hästunderstödd terapi med någon form av psykosocial inriktning kan förstås ur ett sociologiskt perspektiv. Fyra artiklar som behandlar Hästundersstödd terapi analyseras med ett konstruktivistiskt förhållningssätt och en induktiv ansats har antagits. Resultatet av analysen ställs i relation till tidigare sociologisk forskning och sociologiska teorier som behandlar interaktion, kommunikation och relation mellan människa och djur. Resultatet mynnade i tre kategorier som står i relation till varandra. Kategorierna är: Sociala relationer, emotionell utveckling och identitetsskapande. Analysen visar att både det terapeutiska innehållet och målet med den Hästunderstödda terapin i de fyra analyserade artiklarna till största del innefattar sociala relationer. Utifrån detta dras slutsatsen att deltagares upplevelser och effekter av Hästunderstödd terapi låter sig förstås ur ett sociologiskt perspektiv, då sociala relationer är essentiellt inom sociologin.

In this dissertation I analyze how the postwar German writers Wolfdietrich Schnurre, Günter Eich, and Ilse Aichinger negotiate anthropocentric and speciesist discourses via animal figures by drawing on such posthumanist thinkers as Derrida, Agamben, and Deleuze & Guattari. The literary texts question a world view and discourse organized around the establishment of power that utilizes animal metaphors to turn living beings into objects (and could thus be called “carno-phallogocentric”). They thus react to the strict hierarchy of (gendered) man over animal and respond - in the aftermath of the Second World War – by highlighting instead the similarities between man and animal, such as creaturely existence and shared trauma. The analysis is guided by questions such as: How do the literary texts reflect and subvert the power discourses which surround man and animal? What is the role of language in this context? How does the animal, which is usually assumed to be mute, relate to the categories that are established in language? Does its place outside of language grant it capabilities the human cannot realize? Can the literary encounter between man and animal establish a space of the “Open” in which language can be re-evaluated and, after World War II, be saved? Is there a unique “animal poetology” which correlates to post-anthropocentric conceptions of the human? Because these writers disorient the reader’s perception of reality via figures of the animal, i.e., animals as both metaphors and as subjects, I develop what I would like to call an “animal poetology” that is unique to them. This animal poetology, which redefines Agamben’s concept of the open by giving it a postwar, language-critical dimension, includes a thorough critique of human language with regard to power structures and a speciesist language which, during the early 20th century, was a vehicle for ideology and discrimination. The encounter with the animal leads the human being to reflect on the limits of language and thus enables the establishment of a mode of being in which the encounter with the other – beyond a space of judgement and hierarchies –is once again possible.

This project examines small mammal faunas from cave sites in south-west England and south Wales. The aims are threefold: To examine the rapid environmental changes taking place in the Late Pleistocene and early Holocene: To understand the processes by which small mammal remains were deposited in the caves examined: To demonstrate the value of small mammal studies as an archaeological tool. All identifiable small mammal remains from twelve selected sites are listed. Ten of the sites are new material. As the species examined here are seldom exploited by humans, the small mammals provide a record of the past environment unaffected by human selection of particular species, as might be the case in larger mammal assemblages. An examination of possible agents of accumulation is provided for each site to identify any bias introduced by prey selection. Reconstructions of the environment local to each cave at the time of deposition are offered. The evidence provided by the small mammals is related to the archaeological findings from each cave, to demonstrate the effect of human habitation of cave sites on the depositional and post-depositional processes shown by the microfauna. The environmental evidence provided by the study reflects a wider landscape rather than merely the immediate surroundings of the cave, and so gives a basis for human exploitation patterns in the area accessible from the cave. Reconstructions of the ecological mosaics formed by the rapidly changing climate of the period and the topographic variation around the cave sites are provided, demonstrating the potential complexity of the environment in which the humans and other fauna of the period existed. It is hoped that this will encourage archaeologists to look beyond the general division of environmental boundaries in this period, and to examine the local variation in habitat availability and use.

abstract: The objective of this small animal pre-clinical research project was to study quantitatively the long-term micro- and macro- structural brain changes employing multiparametric MRI (Magnetic Resonance Imaging) techniques. Two separate projects make up the basis of this thesis. The first part focuses on obtaining prognostic information at early stages in the case of Traumatic Brain Injury (TBI) in rat animal model using imaging data acquired at 24-hours and 7-days post injury. The obtained parametric T2 and diffusion values from DTI (Diffusion Tensor Imaging) showed significant deviations in the signal intensities from the control and were potentially useful as an early indicator of the severity of post-traumatic injury damage. DTI was especially critical in distinguishing between the cytotoxic and vasogenic edema and in identification of injury regions resolving to normal control values by day-7. These results indicate the potential of quantitative MRI as a clinical marker in predicting prognosis following TBI. The second part of this thesis focuses on studying the effect of novel therapeutic strategies employing dendritic cell (DC) based vaccinations in mice glioma model. The treatment cohorts included comparing a single dose of Azacytidine drug vs. mice getting three doses of drug per week. Another cohort was used as an untreated control group. The MRI results did not show any significant changes in between the two treated cohorts with no reduction in tumor volumes compared to the control group. The future studies would be focused on issues regarding the optimal dose for the application of DC vaccine. Together, the quantitative MRI plays an important role in the prognosis and diagnosis of the above mentioned pathologies, providing essential information about the anatomical location, micro-structural tissue environment, lesion volume and treatment response. / Dissertation/Thesis / Masters Thesis Bioengineering 2014