In this thesis I argue that anarchism, as a political philosophy and social movement practice committed to resisting all forms of oppression and domination, needs to place colonialism more clearly and consistently at the centre of its analysis of interlocking systems of oppression. I argue that colonialism has largely been absent as a system of domination and oppression within anarchist theory and practice, with the exception of a small number of theorists and activist groups. My contention is that an anti-colonial orientation needs to be further encompassed in part because of anarchism’s commitment to resisting all forms of oppression and domination, but also because anarchist movements carry out and theorize resistance against the backdrop of settler colonial realities and on Indigenous lands. This thesis contributes to these aims by seeking to expand and push further work done on anarcha-Indigenism that examines the possible points of contact and resonance between anarchist and Indigenous politics, philosophy and action. I conceptualize anarcha-Indigenism first as a ‘third space’, following post-colonial theorists, and extend this concept towards an ‘n-dimensional’ space of meeting, where theoretical perspectives can come to engage with one another. This space consists of points of contact and resonance chiefly between Indigenist, anarchist and feminist theory and practice, but also extends to any perspective that might meet the core commitments of anarcha-Indigenism, namely resistance to all forms of oppression and domination. Within the ‘n-dimensional’ space of anarcha-Indigenism I examine the possibilities of anti-colonial research methodology, solidarity relationships between settlers and Indigenous peoples, engagements between anarchist and Indigenous feminisms and contemporary manifestations of anti-colonial anarchist resistance against the 2010 Vancouver Olympics and the Toronto G20. These successive chapters seek to make anti-colonial interventions into anarchist theory and practice as well as further develop the richness of anarcha-Indigenism and its complexities. Overall, I argue that anarcha-Indigenism, as an ‘n-dimensional’ space of meeting can further resistance to all forms of oppression and domination, and has the ability to make anti-colonial interventions into anarchist theory and practice specifically. / Thesis (Master, Cultural Studies) -- Queen's University, 2012-09-30 23:49:58.642

No description available.

No description available.

No description available.

No description available.

No description available.

No description available.

No description available.

Monoclonal RhD antibodies (anti-RhD) may potentially be used in preventing Rh alloimmunisation of women delivering RhD-positive babies, and thereby preventing haemolytic disease of the newborn (HDN). Binding and molecular analyses were performed on a range of monoclonal anti-RhDs to understand the interaction between the antibody and antigen. A flow cytometry (FCM) assay system was developed to analyse the binding of the anti-RhD to human group O R₁R₂ red blood cells (RBC). A panel of 30 anti-RhD preparations were studied using this assay and Vmax (maximum binding) and KD (equilibrium constant) were determined for each antibody. The antibodies were categorised into 3 groups (low, medium and high binders) according to their Vmax. Furthermore, the Vmax was converted to the number of antibody molecules bound per RBC (NMBR) by using a correlation curve generated from running parallel FCM and radioiodination assays (RIA). Scatchard analysis of the RIA data indicated that the total NMBR for O R₁R₂ RBC was 27,300 antigen sites/cell. Molecular analysis involved cloning and sequencing of 11 anti-RhD. Heavy chains (HC) preferentially used gene segments from the VH3 and VH4 families, and kappa chain (κ LC) usage was restricted to DPK9. Four sets of antibodies, showed restricted D gene segments (encode part of the HCDR3) which indicated possible importance for epitope specificity. Analysis of V gene sequence indicated that common VH and VL pairings were found used by the medium binders. The high and low binders had unique VH and VL pairings, although the high binders also showed greater somatic mutations from their respective germline genes. It was concluded that the fit of the antibody to the RhD antigen is dependent on both the VH and VL usage and pairing, and that the precise epitope specificity of these antibodies may require HCDR3 interaction.

No description available.