Demand for tea is increasingly driven by its reported antioxidative properties. To ascertain such efficacy, the antioxidative activity (AA) of freshly brewed commercially available full-leaf white, green, oolong, and black tea was determined using a dynamic method. Various amounts (w/v) of tea were brewed at different temperatures for a constant period of time. The AA was calculated based on the ability of the brew to quench hydroxyl-radicals as quantified by chemiluminescence detection. Black tea had the strongest radical scavenging ability followed by green tea. Their AA was far greater than those of the other two types of tea tested. This efficacy finally eroded on serial dilution to a tea concentration of 0.15625 X 10-4 g/mL. The significant data clearly substantiate the sound premise that tea, particularly black tea, is unique in its dramatic ability to counter the adverse onslaught of radicals that are known contributors to human morbidity and mortality.

Turmeric polar extract (TPE) was obtained by dielectric-precipitation of turmeric slurry and found to contain three proteins with two in the 10-11 KDa range being dominant. Antioxidative activity and persistence (AP) of TPE (5%, w/v) respectively showed 87% and 85% greater generation of alkoxy- and peroxyl radicals compared the non-redox-active buffer alone showing significant (p<0.05) pro-oxidative behavior. Conversely, purified curcumin (CU) (0.1% w/v) was dramatically antioxidative with AA and AP values of 2,828 and 1,129%, respectively, compared to the blank. However, a combination of the two at the same concentration dropped these values to 590 and 389%, respectively, reflecting dramatic dampening of the efficacy of CU. Ultraviolet radiation significantly modulated the efficacy of CU where UVB (300 nm) exposure gave the highest enhancement when limited to five min. Data showed that turmeric contains highly pro-oxidant polar proteins that significantly dramatically diminishes the beneficial antioxidative efficacy of its principal phytochemical, CU.

Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Botany and Ecology Candidate: Bc. Blanka Kaluzsná Supervisor: Prof. RNDr. Lubomír Opletal, CSc. Title of Diploma Thesis: Biological activity of plant metabolites XXIII. alkaloids of Corydalis cava (l.) Schweigg. & Körte and their effect on acetylcholinesterase and butyrylcholinesterase An ether extract which contained tertiary basic alkaloids was prepared from the dry bulb of Corydalis cava (L.) SCHWEIGG. & KÖRTE. A subfaction labeled 2/B was obtained by column chromatography and crystallization. Three alkaloids were isolated by preparative TLC and they were identified by GC/MS and 1 H a 13 C NMR analyzes as (+)-canadine, (+)-tetrahydropalmatine and domestine. Isolated alkaloids were tested by spectrophotometric Ellman method for inhibitory activity against human erythrocyte acetylcholinesterase (HuAChE) and plasma butyrylcholinesterase (HuBuChE). In comparison with the refernce substances, from these isolated alkaloids only (+)-canadine showed an relatively high inhibitory activity against HuAChE (12,4 µM). (+)-Tetrahydropalmatine and (+)-domestine had no significant inhibitory activity in this direction, against both HuAChE and HuBuChE. Key words: Corydalis cava, (+)-canadine, (+)-tetrahydropalmatine,...

Tyrimo tikslas - išnagrinėti „BRONCHOS“ arbatų linijos („Bronchos“ vaistažolių arbata, „Bronchos Nakčiai“ žolelių arbata, „Bronchos-1 vaistažolių arbata“, „Bronchos Forte“ arbata) sudėtis ir atlikti jų palyginamąją analizę, vertinant bendrą fenolinių junginių kiekį ir antioksidacinį aktyvumą. Tyrimo metodai. Bendras fenolinių junginių kiekis visose tirtose „BRONCHOS“ arbatose nustatytas spektrofotometrijos metodu pagal galo rūgšties standartą. Antioksidacinis aktyvumas įvertintas spektrofotometriškai naudojant DPPH radikalų sujungimo metodą. Literatūros analizės metodas naudotas „BRONCHOS“ arbatų linijos sudėčių analizei. Gauti rezultatai apdoroti naudojant Microsoft Office Excel 2013 ir Sigma Plot 12.0 programas. Tyrimo rezultatai ir išvados. Nustatyta, kad arbata „Bronchos Forte”, lyginant su kitomis tirtomis arbatomis, pasižymi stipresniu veikimu pagal bendro fenolinių junginių kiekio ir antioksidacinio aktyvumo tyrimų rezultatus. Didžiausias fenolinių junginių kiekis nustatytas tiriant arbatų nuovirus, o stipriausios antioksidacinės savybės būdingos gamintojo rekomenduojama metodika paruoštoms vandeninėms arbatų ištraukoms. Atlikti tyrimai rodo, kad ekstrahavimo laikas daro neigiamą įtaką „BRONCHOS“ arbatų kokybei, išskyrus, kai šių arbatų ištraukos ruošiamos naudojant 20 °C temperatūros vandenį. / The aim of this research is to examine the composition of the line of ‘Bronchos’ teas (‘Broncho’ herbal tea, ‘Broncho Night’ herbal tea, ‘Broncho -1’ and ‘Broncho Forte’ herbal tea) as well as to perform the comparative analysis in order to evaluate the quantity of total phenolic compounds and antioxidative activity. Methods. The total amount of phenol compounds in ‘Bronchos’ teas is estimated spectrophotometrically. Galic acid was used as a standart. Antioxidative activity was determined spectrophotometrically by DPPH radical scavenging method. The analysis of the literature was used in order to examine the composition of the line of ‘Bronchos’ teas. The results were obtained using Microsoft Office Excel 2013 and Sigma Plot 12.0 programs. The results and conclusions of the analysis. Based on the results of the phenolic compounds and antioxidative activity analysis, ‚Broncho Forte‘ tea has the biggest therapeutic effectiveness compared to the other brands that have been investigated. The biggest amount of phenolic compounds has been indicated when tea decoction process has been investigated, while aqueous tea extracts prepared according manufacturer’s recommendations have the strongest antioxidative features. Studies have also shown that extraction time adversely affects the quality of ‚BRONCHOS‘ tea, unless tea extracts are produced using water of 20 °C degree.

<p>Sintetisana su nova jedinjenja, 16- i 17-supstituisani&nbsp;16,17-sekoestratrienski derivati i D-homoestranski&nbsp;derivati, polazeći od 3-benziloksi-17-hidroksi-16,17-sekoestra-1,3,5(10)-trien-16-nitrila. Ispitana je&nbsp;estrogena i antiestrogena aktivnost u eksperimentima <em>in&nbsp;vivo</em>, antiaromatazna aktivnost <em>in vitro</em>, antioksidantna&nbsp;aktivnost DPPH &nbsp;i TBA testom, kao i antiproliferativna&nbsp;aktivnost prema ćelijskim linijama MCF-7 ATCC,&nbsp;MDA-MB-231, HT-29 i MRC-5 novosintetisanih&nbsp;jedinjenja.</p> / <p>Some new compounds, 16- and 17-substituted 16,17-secoestratriene derivatives, as&nbsp; well as D-homoestratriene derivatives, were synthesized, starting from 3-benzyloxy-17-hydroxy-16,17-secoestra-1,3,5 (10)-triene-16-nitrile. The newly synthesized compounds were tested for their <em>in vivo&nbsp;</em>estrogenic and antiestrogenic activity,&nbsp; <em>in vitro&nbsp;</em>antiaromatase activity, antioxidative activity by DPPH and TBA tests, as well as antiproliferative activity against MCF-7 ATCC, MDA-MB-231, HT-29 i MRC-5 cell lines.</p>