8. Experiment pursued stimulators for propagation of Japanese Azaleas. Mother plants of varieties of Japanese Azaleas species 'Oslava' and 'Diamant Alba' were based on three sites : outside, in the coldframe (overshadowed during summer period) and in the greenhouse. 55 pieces of the species 'Oslava' were planted, 19 pieces of the species 'Diamant Alba' were planted in the greenhouse and 35 pieces in the coldframe and outside. During winter time some of the plants got frozen. Simultaneously the experiment was extended in the second vegetation period by new mutation called 'Rubinstern'. The experiment proved the best rooting accomplishments were given by species 'Oslava'. Species 'Diamant Alba' gave quite weak results of rooting caused mainly by low cold resistance. The worst results of rooting were given by the species 'Rubinstern'. The trial confirmed, that the best of the cultivar, from rooting point of view, is mutation 'Oslava'. Cultivar 'Diamant Alba' was showed poor rooting process, because of its lower freeze resistence. The worst results of rooting were given by the species 'Rubinstern'. In the first vegetative period the parent plants from outside of a low growth which is a reason why it's being left without mentioning the quantity. The AS-1 stimulator was used and the experiment was even enhanced to treatment applying IBA and non-stimulating control. The results showed that used IBA, AS -- 1 stimulators and the non -- stimulated control gave very similar results. No statistical difference was found between the first and the second iterancy in 2006. After heaping up some loose soil, the cuttings that had 5 or more roots reached the best fixation. Achieved reproduction yield found at the type termed 'Oslava' proves, that there is a real option of using stimulators for rooting of cuttings in the regular practice of agricultural comp. in Jeseník.

The TIR/BB-loop mimetic AS-1 has been reported to prevent cardiac hypertrophy by inhibiting interleukin-1 receptor (IL-1R)-mediated myeloid differentiation primary response gene 88 (MyD88)-dependent signaling. To date, it remains unknown whether and if so how AS-1 contributes to mechanical stress (MS)-induced cardiac fibroblast activation, a key process in pressure overload-induced cardiac remodeling and heart failure. Here, we show that phosphorylation and expression of large tumor suppressor kinase 1 (LATS1), a key molecule in the Hippo-Yes associated protein (YAP) signaling pathway, were down-regulated in primary neonatal rat cardiac fibroblasts (NRCFs) in response to MS and in the hearts of mice subjected to transverse aortic constriction (TAC) procedure; AS-1 treatment was able to restore LATS1 phosphorylation and expression both in vitro and in vivo. AS-1 treatment suppressed the induction of proliferation, differentiation and collagen synthesis in response to MS in NRCFs. AS-1 also ameliorated cardiomyocyte hypertrophy and apoptosis through dampening paracrine secretion of stretched cardiac fibroblasts. In mice, AS-1 treatment could protect against TAC-induced cardiac hypertrophy, myocardial fibrosis and heart failure. Of note, LATS1 depletion using siRNA completely abrogated the inhibitory effects of AS-1 on NRCFs under MS including accelerated proliferation, differentiation, enhanced ability to produce collagen and augmented paracrine secretion of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) to induce cardiomyocyte hypertrophy. Therefore, our results delineate a previously unrecognized role for LATS1 in cardiac fibroblast to mediate the beneficial effects of AS-1 in preventing pressure overload-induced cardiac remodeling and heart failure.

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