The research was carried out in 2005 at the Lithuanian University of Agriculture, Department of Horticulture. The aim of research was to investigate the yield and quality of different leeks cultivars as influenced by different grown methods. Leeks were growing in these ways: I way – growing in smooth surface; 2 way – growing into 15 cm deep furrows; 3 way - growing on the 15 cm furrows; 4 way – growing in the holes of 20 cm depth. The biggest harvest has been received from the early leek varieties, total harvest - 36,63 t ha-1, standard harvest – 31,12 t ha-1 and late leek varieties total harvest - 26,85 t ha-1, standard harvest – 24,05 t ha-1) grown in smooth surface. The early leeks grown in the holes of 20 cm depth has been received the biggest mass per plant (average 325,0 g) and mass per plant of the late leeks has been received from the leeks grown on the 15 cm furrows (average 274,7 g). The early leeks of the best biochemical composition have been grown in the holes of 20 cm depth and the late leeks have been grown on the 15 cm furrows.

Problem. On the present time both Lithuania and EU market dominate pigs and cattle breeding. Consumer researches shows, that growing demand on high digestibility and nutrient, quick prepare animal products. On this way duck meat is very valuable product. Problem is that now dominate small farms, where traditionally growing few local, not very productive ducks. Like there is no real local market, so there is no well develop duck breeding. The subject of research. After good analyses of duck breeding and risk factors, to take measures of duck business development in Lithuania. Tasks: To estimate different duck species peculiarity and analyse duck meat quality depend to market demand; To estimate duck breeding technology and use in Lithuania farms; To estimate risk and possibility of duck business in Lithuania. Results. Consumer researches shows, that people prefer not fat, fragile, muscular duck meat. These features satisfy meat duck mularde females. From a technological viewpoint duck is insensitive to temperature, moisture, feed changes, so it’s possible to grow them both intensive and extensive farms. Technological, zoohigienic and economic duck growing research showed that the most promising duck breeding is like part business – on the summer time use empty animal husbandry buildings. At best is to grow heterozic hybrids. These precondition enable in Lithuania to create small duck farms, which sells fresh meat straight from the farm and secure flexible sells and... [to full text]

Magistro darbe tiriama skirtingų auginimo technologijų įtaką juodiems serbentams. Darbo objektas – ekologiškai ir įprastai auginami juodieji serbentai (‘Ben Lomond‘, ‘Ben Alder‘, ‘Ben Tiran‘). Darbo tikslas - ištirti ekologiškai auginamų juodųjų serbentų derėjimą bei uogų kokybę ir palyginti su įprastai auginamais juodaisiais serbentais. Darbo metodai – lauko tyrimai (buvo skaičiuojamas stiebų kiekis viename krūme, kekių skaičius krūme, žiedų ir uogų skaičius kekėje, krūmo derlingumas) ir laboratoriniai tyrimai (vitamino C ir sausųjų medžiagų kiekis uogose). Darbo rezultatai. Nustatyta, kad įprastiniu būdu auginamų serbentų veislė ‘Ben Lomond‘ turėjo iš esmės daugiau kekių viename krūme, žiedų ir uogų kekėje, lyginant su kitomis tirtomis veislėmis. Auginimo technologijos neturėjo esminės įtakos vidutinei uogos masei. ‘Ben Lomond‘ veislė auginama tiek ekologiškai tiek ir įprastai turėjo iš esmės didžiausią esamą ir potencialų vieno krūmo derlių ir derlingumą t ha-1. Daugiausiai sausųjų medžiagų sukaupė iš esmės ‘Ben Lomond‘ veislės serbentų uogos auginamos įprastiniu būdu. Statistiškai patikimai daugiausiai vitamino C sukaupė ekologiškai auginamos ‘Ben Alder‘ veislės uogos. / Master‘s work deals with influention of different growing technologies on blackcurrant. Object of work – blackcurrant (‘Ben Lomond‘, ‘Ben Alder‘, ‘Ben Tiran‘) grown ecologically and conventionally. Purpose of work is to investigate the productivity and the quality of ecologically grown blackcurrant comparing with conventionally grown blackcurrants. Methods of work – field investigations (counting amount of stems in one bush, number of trusses in a bush, number of blossoms and berries in a truss and the productivity of a bush) and laboratory research (amount of vitamin C and dry substances in berries). Results of work. It is established that conventionally grown blackcurrant ‘Ben Lomond‘ has substantially had more trusses in a bush as well as blossoms and berries in a truss comparing with other investigated species. Growing technologies have not had essential influence on average mass of a berry. ‘Ben Lomond‘ species grown both ecologically and conventionally has had the largest present and potential productivity and harvest t ha-1. The largest amount of dry substances has been accumulated in blackcurrant species ‘Ben Lomond‘ grown conventionally. Statistically reliably the largest amount of vitamin C has been accumulated in ecologically grown blackberries of ‘Ben Alder‘ species.

Human mesenchymal stem cells (MSC) have attracted a great deal of interest for their potential use in regenerative medicine and suppression of the inflammation. Nevertheless, all known therapy protocols require large amounts of MSCs, which can be obtained only by in vitro expansion. One of the most important methodological problems is associated with the use of animal-derived components in the cell culture medium. The main aim of the current research was to elucidate the influence of different serum substitutes on the proliferation, differentiation, expression of cell surface markers, and total protein expression of mesenchymal stem cells derived from human adipose tissue. In addition we were aiming to determine the features of mesenchymal stem cell populations from an exfoliated deciduous tooth (SHED) and their response to the multifunctional proinflammatory protein alpha1-antitrypsin. Our results indicate that adipose tissue derived MSCs cultivated in the presence of fetal calf serum and allogeneic human serum display similar properties, while synthetic serum substitute induces increase in growth and differentiation potential of MSCs. Moreover, our results indicate, that synthetic serum substitute also activates transcription of genes related to adipogenic and osteogenic differentiation and diminishes expression of cell surface marker CD146. In the present study, we used a proteomic approach that allowed us to compare protein expression signatures between primary cell... [to full text] / Žmogaus suaugusio organizmo mezenchiminės kamieninės ląstelės (MKL) pradėtos sėkmingai naudoti pažeistų audinių regeneracijai ir uždegiminio proceso slopinimui. Šiuolaikiniams terapijos protokolams yra reikalingi dideli ląstelių kiekiai, todėl prieš naudojimą yra būtina jas padauginti in vitro. Tačiau naudojant skirtingus MKL išskyrimo, in vitro kultivavimo ir padauginimo protokolus, yra sunku lyginti mokslinių tyrimų duomenis ir klinikinių tyrimų rezultatus. Nevienodos kultivavimo sąlygos gali įtakoti MKL funkcines savybes (augimo greitį, gebėjimą diferencijuotis, migracinį aktyvumą ir kt.). Šiame disertaciniame darbe tyrėme auginimo terpių, praturtintų skirtingais serumais (gyvulinės kilmės serumai, žmogaus alogeninis serumas, sintetinis serumas), poveikį žmogaus riebalinio audinio MKL. Taip pat charakterizavome MKL išskirtas iš žmogaus pieninių dantų pulpos ir palyginome šių ląstelių kultūros bei jų dukterinių klonų savybės. Kompleksiškai tyrėme uždegimo antiproteinazės alfa1-antitripsino (AAT) poveikį šioms ląstelėms. Nustatėme, kad, lyginant su kitais tyrime naudotais serumais, sintetinis serumo pakaitalas geriausiai skatino MKL augimą bei gebėjimą diferencijuotis adipogenine ir osteogenine kryptimis. Taip pat, skirtingai nuo kitų naudotų gyvulinės kilmės ir žmogaus serumų, sintetinis serumo pakaitalas veikė kaip adipogeninės ir osteogeninės diferenciacijos indukcijai svarbių genų PPARγ ir Msx2 transkripcijos aktyvatorius. Be to, sintetinis serumo pakaitalas slopino... [toliau žr. visą tekstą]