The aim of this study was to evaluate research of reproduction indicators on the level of milk production and the quality of the milk on selected farm. Materials for processing thesis were obtained on Oldřich Poláček´s Farm Hole. Mr. Poláček´s farm is located in Central Bohemia and manages 380 ha of arabe land. The farm bred Holstein cows, for now there are 190 dairy cows of this breed. The indicators of milk production and reproduction were observed from 2013 to 2015. Results of the observed farm were compared with results of milk yield of Holstein cattle breeders association in the Czech Republic. The data were analyzed and statistically processed using SAS 9.3 (SAS / STAT; 9.3, 2011). To determine the basic parameters of the files were used MEANS procedures and UNIVARIATE. Evaluation of milk yield and indicators of reproduction was based on the results obtained from the recorded milk production of cows done by Holstein cattle breeders association and stable computer system AFIFARM. To evaluate the data were used 104 dairy cows, which had last two standardized lactation completed in the test years from 2013 to 2015 and 121 dairy cows, which had the first standardized lactation completed in 2014 or 2015. For the milk yield were evaluated parameters of kilograms of milk per lactation, kilograms of milk per day, fat (%), protein (%) milk, depending on reproduction (days open, calving interval, age at the first calving) and lactation order. Milk yield on the farm in 2015 was above the national average in Czech Republic. For dairy cows with the first lactation was up to 8 536 kilograms per standardized lactacion, for dairy cows with second lactation was up to 10 928 kg, and for dairy cows with third and more lactation was up to 10 864 kg. The average content of 3,71% fat and 3,28% protein. The influence of lactation negatively impacted % protein (r = -0.278). Conclusiveness was on the significance level (P <0.001). The highest protein content was on the first lactation 3,40%, and lowest in the fifth lactation 3,20%. Statistically failed to demonstrate the influence of the order of lactation on the milk yield per standardized lactation, kilograms of milk and % of fat content. The positive effect was shown between milk yield per lactation in kg and daily milk yield in kg (r = 1). Conclusiveness was on the significance level (P <0,001). The lowest daily milk yield on the first lactation was (30,24 kg) with the production of 9223,06 kg of milk and the highest on the third lactation (47,43 kg) with the production of 14466,76 kg of milk. Increasing of milk production per lactation had a negative effect on the % of fat content (r = -0,137), and % of protein content (r = -0.196). Conclusiveness was on the significance level (P <0.05). With increasing milk production declined % of fat content and % of protein content. Statistically failed to demonstrate the influence of the milk production per lactacion on the open days and the calving interval. Effect was demonstrated between the % of fat content and protein content in % (r = 0,501) at a level of significance (P <0,001). With increasing of fat content in%, increased also the the protein content in %. The lowest protein content (3,20%) was with a 3,66% of fat content. The highest protein content (3,40%) was with a 3,68% of fat content. Age at first calving of dairy cows negatively impacted on % of the fat content of milk (r = -0,186). Conclusiveness was on the significance level (P <0,05). With age increasing at first calving declined % of fat content in milk. There was also demonstrated a connexion between the standardized lactacion and days open (r= 0,227). Conclusiveness was on to the level of significance (P <0,05). With open days increasing the amond of milk per lactacion was higher.

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The subject of this dissertation pertains to the importance of the consumption of protein and calcium in the diet. The theoretical section is a comprehensive study of protein and calcium, detailing their composition and function, taking into consideration the essential nature of the nutrients in the human body. Sources of protein and calcium from both animals and plants are thoroughly examined. Special attention is given to factors and foods which influence the absorption of protein and calcium in the human body. Discussed is the relationship between the nutrients and the repercussions of their inappropriate proportions in our organism. The dissertation examines the correct amount of the nutrients, and illness and organ malfunction connected with the deficiency of protein and calcium. The practical section of the dissertation looks at the eating habits of the clients of a fitness club. In the world of fitness centres there is an abundance of dietary advice, the majority of which does not correspond to general nutritional recommendations, and as such can have a negative effect on health and well being. Quantitative research in the form of questionnaires focuses on the consumption of protein and calcium not just specific to members of fitness centres. Evaluated are the overall eating habits and...

Objective of my graduate work is tracing of influence quality meat products and their constitution according of used raw materials on highness of production. By the chosen assortment of meat products was i fixated on their quality and monitoring of change dimension raw materials according substitution in meat production.

This master‘s thesis deals with the analysis of 65 different beers, with a focus on finding differences between Czech tap ales, lagers Czech, Czech special beers and foreign beers. Czech beers were compared by the results of analysis among beers bearing the indication "Czech beer" and the group without this indication. The total amount of proteins, polyphenols and carbohydrates was determined using UV-VIS spectrometry. The history of brewing, the nature and types of Czech beers and protected geographical indication "Czech beer" were described. Raw materials for beer production and brewing technology have been listed and described. In the last part of the research, components of beer and the principle of the UV-VIS spectrometry have been specified. Th determination of total protein was performed using Hartree-Lowry method, which is based on two-component reagent. The first component is the biuret agent, the second component is the Folin-Ciocalteau reagent for phenols. Determination of the polyphenols has been carried out by the Folin-Ciocalteau reagent and total carbohydrates were determined spectrophotometrically according to the Analytica EBC using anthron agent. The results were statistically processed and evaluated in the final part.

This diploma thesis is focused on the analysis of selected quality parameters of milk from two breeds of dairy cattle during a year. The literary review contains the information about the basic structure of cow's milk and with a more detailed focus on ingredients, which were observed in this thesis (proteins, fat, lactose, chlorides and somatic cells). In addition, the information was also included on basic milk quality indicators and the information about breeds of dairy cattle from which came samples of milk. Samples for analysis came from dairy cows of Czech Fleckvieh breed (farm GenAgro Ricany, a.s.) and from dairy cows of Holstein breed (University farm Zabcice). Samples of milk were obtained during 4 seasons, namely from January 6th 2017 to December 7th 2017. Analysis of variance (ANOVA) showed that the content of the milk observed components is influenced besides else factors, also by the season (in the winter protein content achieved values on averaged 3,31 % and 3,19 % in the summer). Moreover, a significant negative correlation coefficient was detected between proteins and somatic cell count (r = -0,73) and between lactose and somatic cell count (r = -0,45). Overall results showed that all milk samples meet the quality limits (proteins ≥ 2,8 %, fat ≥ 3,3 %, SCC ≤ 400 103.ml-1).

Study of Plasma Protein Binding of Radiopharmaceuticals Summary The purpose of this work is the study of binding of substances (177 Lu-DOTA- [Lys3]bombesin, 177 Lu-NOTA-[Lys3]bombesin, 177 Lu-PCTA-[Lys3]bombesin, and 177 Lu- DOTA-MG47) to plasma proteins by equilibrum dialysis in 37řC, particularly using plasma samples of beef, rabbit, rat and human. Within this group, these substances were compared interspecifically. The substances 177 Lu-DOTA-[Lys3]bombesin, 177 Lu-NOTA-[Lys3]bombesin, 177 Lu- PCTA-[Lys3]bombesin, and 177 Lu-DOTA-MG47 are the newly developed receptor- specific radiolabeled peptides. For all the newly collected data, the interspecific comparison and subsequent statistical evaluation was performed. The indicated bombesin derivates were compared and statistically analyzed even between themselves. During the interspecies comparisons and the determination of the statistical significance of the data, there were found statistically significant and statistically highly significant differences between some of the examined samples. A highly significant difference was found during comparing with samples of 177 Lu-NOTA- [Lys3]bombesin and statistical evaluation, there was found a statistically highly significant difference. Despite the differences found, it is clear that the plasma binding concerning...

The main purpose of this diploma thesis was to study legislative requirements about marking food products with nutrition labels and summarize the activities and procedures of supervising organizations connected to problematic of goods marking. One of the goals was to briefly describe technological and producing methods of selected food - ham, cheese, kephir milk, baby food with fruit, wafer, bread, wholemeal puffed bread and chocolate. Next goal was to evaluate the risks of food consumption with deceptive nutrition labels. In the practical part of this thesis I have determined quantity of saccharides, lipids, proteins and salt in selected food products by standard methods. Simple saccharides were determined by Schoorl's method and starch volume by Ewers's method. Lipids were determined by Soxhlet's extraction and after put through derivatization. In gas chromatograph (GC) were detected fatty acids. Apart from the Soxhlet's extraction was made also extraction according to Hara and Radin (1987) for comparison. Proteins were determined by method using nitrogen according to Kjehdal with adjustments according to Winkler. The amount of salt was detected analytically by argetometric precipitating titration. Determination the volume of nutritive parameters was made in three ranks of selected products. From obtained results were evaluated possible risks, which might be caused by consumption of food products with deceptively stated nutritive labels.

The aim of the thesis was to determine the content of pure tuber proteins, patatin relative abundance in total tuber protein, and subsequently its antioxidant potential. The theoretical part dealt with general potato characteristics, potato tuber chemical composition and description of analyzed cultivated and wild potato species. In the practical part results of laboratory analysis were presented. It was determined statistically significant genotype variability of antioxidant activity for potato tuber protein as well as for potato tuber dry matter here ranging from 0.221 (Solanum berthaultii 07S0300031) to 0.0066 mg of ascorbic acid/g dry matter (Solanum tuberosum cultivar Kuras). Any direct relationship between potato tuber protein content or patatin relative abundance and antioxidant activity was found. The findings are compared with the literature.