In order to fully understand the third order nonlinear optical response of materials under high irradiance excitation it is necessary to study the temporal and polarization dependence of nonlinear refraction and absorption. There are several existing approaches such as Z-scan and pump-probe techniques to determine those responses. As part of this work, these approaches will be briefly outlined before presenting beam deflection, applied from photothermal beam deflection, as an alternative experimental technique to determine the nonlinear refraction with its temporal and polarization dynamics. This technique measures the angle of the probe beam deflected via the index gradient of the material induced by strong excitation beam, to determine both the sign and magnitude of the nonlinear refraction. The temporal and tensor properties of the nonlinear refractive index can be determined by introducing a delay line, and by varying the polarization of the excitation and probe beam, respectively. To demonstrate the practicality of the beam deflection technique, we performed measurements on Fused Silica, Carbon Disulfide and Zinc Oxide. Each of these samples shows quite different nonlinear responses. Amorphous fused silica exhibits nonlinear refraction purely from instantaneous electronic contribution; while Carbon Disulfide shows a much slower response, originating not only from the electronic contribution but also from non-instantaneous nuclear movements (e.g. molecular orientation). These two contributions can be separated by varying the polarization direction of the excitation and probe beam. By introducing lock-in detection technique, a sensitivity of λ/5500 can be achieved. In Zinc Oxide, a wide-bandgap semiconductor, we measure both nonlinear refraction and two-photon absorption simultaneously. Therefore the beam deflection is a sensitive technique, which can be used to measure the time and polarization dynamics of the nonlinear response of the material

Aus der Einleitung: "Das selektive Elektronenstrahlschmelzen (engl. Electron Beam Melting (EBM®)) ist ein pulverbettbasiertes additives Fertigungsverfahren, mit dessen Hilfe metallische Bauteile schichtweise hergestellt werden können. Der schematische Aufbau einer entsprechenden Anlage ist in Abbildung 4 dargestellt. Dabei erfolgt die Strahlerzeugung im Bereich 1 (die Kathode besteht entweder aus Wolfram oder bei den neuesten Systemen aus einkristallinem LaB6). Die Strahlablenkung durch ein elektromagnetisches Linsensystem erfolgt im Bereich 2. Der Bereich 3 ist die eigentliche Baukammer, in der sich unter anderem die Vorratsbehälter für das Pulver, das Rakelsystem sowie die Komponenten des Bauraums (Käfig mit Hitzeschild, Bauplattform mit Startplatte) befinden. ..."

This thesis deals with product design rotating camera crane with variable length telescopic arm. Proposed maximum arm length is 6 [m] with a maximum weight capacity of the camera 8 [kg]. The work deals with the various structural units associated mainly with the issue of the telescopic arm. In individual sections detail the construction of the camera head developed deformation and stress analysis of a telescopic jib.

Presented in this work are the results of twelve flexural tests conducted on small-scale coupons to establish the load-deflection behavior of UNS S32003 (ATI 2003®) hot-rolled duplex stainless steel flat plates. All specimens were tested as simply supported beams loaded at the midspan. Test specimens had nominal width and thickness of 1 in. and 0.25 in., respectively. Four different span lengths of 4 in., 6 in., 9 in., and 12 in. were investigated. Analysis of the results showed that the non-linear deflection behavior can be estimated reasonably well by adopting the conventional deflection equation pertaining to an assumed linear elastic material, but after replacing the modulus of elasticity with a secant modulus corresponding to the maximum tension strain resulting from the applied load.

No description available.

Aus der Einleitung: "Das selektive Elektronenstrahlschmelzen (engl. Electron Beam Melting (EBM®)) ist ein pulverbettbasiertes additives Fertigungsverfahren, mit dessen Hilfe metallische Bauteile schichtweise hergestellt werden können. Der schematische Aufbau einer entsprechenden Anlage ist in Abbildung 4 dargestellt. Dabei erfolgt die Strahlerzeugung im Bereich 1 (die Kathode besteht entweder aus Wolfram oder bei den neuesten Systemen aus einkristallinem LaB6). Die Strahlablenkung durch ein elektromagnetisches Linsensystem erfolgt im Bereich 2. Der Bereich 3 ist die eigentliche Baukammer, in der sich unter anderem die Vorratsbehälter für das Pulver, das Rakelsystem sowie die Komponenten des Bauraums (Käfig mit Hitzeschild, Bauplattform mit Startplatte) befinden. ..."

A mathematical dynamic model is derived for the compliant constant-force mechanism, based on the pseudo-rigid-body model simplification of the device. The compliant constant-force mechanism is a slider mechanism incorporating large-deflection beams, which outputs near-constant-force across the range of its designed deflection. The equation of motion is successfully validated with empirical data from five separate mechanisms, comprising two configurations of compliant constant-force mechanism. The dynamic model is cast in generalized form to represent all possible configurations of compliant constant-force mechanism. Deriving the dynamic equation from the pseudo-rigid-body model is useful because every configuration is represented by the same model, so a separate treatment is not required for each configuration. An unexpected dynamic trait of the constant-force mechanism is discovered: there exists a range of frequencies for which the output force of the mechanism accords nearer to constant-force than does the output force at static levels.

A novel optical diffraction based technique is proposed and demonstrated to measure deflections of the order of ~1nm in microcantilevers (MC) designed for sensing ultra-small forces of stress. The proposed method employs a double MC structure where one of the cantilevers acts as the active sensor beam, while the other as a reference. The active beam can respond to any minute change of stress, for example, molecular recognition induced surface stress, through bending (~1nm) relative to the other fixed beam. Optical diffraction patterns obtained from this double slit aperture mask with varying slit width, which is for the bending of MC due to loading, carries the deflection profile of the active beam. A significant part of the present work explores the possibility of connecting diffraction minima (or maxima) to the bending profile of the MC structure and thus the possibility to measure induced surface stress. To start with, it is also the aim to develop double MC sensors using PHDDA (Poly – Hexane diol diacrylate) because this material has the potential to achieve high mechanical deformation sensitivity in even moderately scaled down structures by virtue of its very low Young’s modulus. Moreover, the high thermal stability of PHDDA also ensures low thermally induced noise floors in microcantilever sensors. To demonstrate the proposed optical diffraction-based profiling technique, a bent microcantilever structure is designed and fabricated by an in-house developed Microstereolithography (MSL) system where, essentially one of the microcantilevers is fabricated with a bent profile by varying the gap between the two structures at each cured 2D patterned layer. The diffraction pattern obtained on transilluminating the fabricated structure by a spherical wavefront is analyzed and the possibility of obtaining the deflections at each cross section is ascertained. Since the proposed profiling technique relies on the accurate detection and measurement of shifts of intensity minima on the image plane, analysis of the minimum detectable shift in intensity minima for the employed optical interrogation setup with respect to the minimum detectable contrast and SNR of the optical measurement system is carried out, in order to justify the applicability of the proposed minima intensity shift measurement technique. The proposed novel diffraction based profiling technique can provide vital clue on the origins of surface stress at the atomic and molecular level by virtue of the entire bent profile due to adsorption induced bending thereby establishing microcantilever sensor technology as a more reliable and competitive approach for sensing ultra-low concentrations of biological and chemical agents.

Downstream regulatory antagonist modulator (DREAM) is a calcium sensing protein that co-assembles with KV4 potassium channels to regulate ion currents as well as with DNA in the nucleus, where it regulates gene expression. The interaction of DREAM with A-type KV4 channels and DNA has been shown to regulate neuronal signaling, pain sensing, and memory retention. The role of DREAM in modulation of pain, onset of Alzheimer’s disease, and cardiac pacemaking has set this protein as a novel therapeutic target. Moreover, previous results have shown a Ca2+ dependent interaction between DREAM and KV4/DNA involving surface contacts at the N-terminus of DREAM. However, the mechanisms by which Ca2+ binding at the C-terminus of DREAM induces structural changes at the C- and N-terminus remain unknown. Here, we present the use of biophysics and biochemistry techniques in order to map the interactions of DREAM and numerous small synthetic ligands as well as KV channels. We further demonstrate that a highly conserved network of aromatic residues spanning the C- and N-terminus domains control protein dynamics and the pathways of signal transduction on DREAM. Using molecular dynamics simulations, site directed mutagenesis, and fluorescence spectroscopy we provide strong evidence in support of a highly dynamic mechanism of signal transduction and regulation. A set of aromatic amino acids including Trp169, Phe171, Tyr174, Phe218, Phe235, Phe219, and Phe252 are identified to form a dynamic network involved in propagation of Ca2+ induced structural changes. These amino acids form a hydrophobic network connecting the N- and C-terminus domains of DREAM and are well conserved in other neuronal calcium sensors. In addition, we show evidence in support of a mechanism in which Ca2+ signals are propagated towards the N-terminus and ultimately lead to the rearrangement of the inactive EF-hand 1. The observed structural motions provide a novel mechanism involved in control of the calcium dependent KV4 and DNA binding. Altogether, we provide the first mechanism of intramolecular and intermolecular signal transduction in a Ca2+ binding protein of the neuronal calcium sensor family.

Tato práce se zabývá využitím distribucí neboli zobecněných funkcí k řešení nestacionárních okrajových problémů v mechanice kontinua. Nejprve je zavedena teorie distribucí a jejich definice jako spojitých lineárních funkcionálů na prostoru testovacích funkcí. Druhá část teoretické kapitoly představuje Laplaceovu integrální transformaci. Následující kapitola se věnuje řešení průhybu nosníků pod vlivem nespojitého časově proměnlivého zatížení. Jejím výsledkem je vytvoření obecného modelu řešení průhybových čar nosníků vužitím distribucí. Poslední kapitola se zabývá řešením nestacionárního proudění v trubicích spojených hydraulickými prvky.