Amount of rutin and quercetin in bark and leaves from different type of Sambucus nigra was determined in this diploma thesis. Records of amounts were compared with amounts in significant sources of these flavonoids. Matters from raw matrix were obtained with pressurized solvent extraction (PSE) and pressurized hot water extraction (PHWE). There was use temperature 120°C, pressure 150 bar, three times 5-min cycles for PSE. There were used 80°C, 150 bar, three 5-min cycles for rutin extracted with PHWE and the same condition were used for extraction of quercetin only the temperature was changed to 100 °C. Analysis of rutin and quercetin were carry out high performance liquid chromatography (HPLC) with diode array detection (DAD), where was used SUPELCOSILTM LC 8DB column (5µm; 250 x 4.6 mm). Methanol: water: formic acid (36: 61.5: 2.5, pH 2.17 2.28) was used like a mobile phase. The flow of the mobile phase was set up 0.7 ml/min. Size of sample was 10 µl. The most amount of rutin was determined in the leaves of wild kind of Sambucus nigra with methanol extraction and hot water too. Extraction with PHWE was more effectively. There were determined 5.58 mg of rutin in one gram of sample from wild Sambucus nigra. There were determined the smallest amount of rutin in Sambucus nigra Körsör. There was found only 0.13 mg/g with extraction PHWE. The quercetin wasn’t determined in any Sambucus nigra leaves. There were found both of the flavonoids (rutin and quercetin) in the branch of Sambucus nigra. The rutin was determined in the small amount in wild kind but it was found in the important amount in Sambucus nigra cultivar. Whereas the quercetin was found in higher concentration than rutin when was finding the ideal conditions for extraction of branch. The quercetin was chosen like more important in light of amount. In the end the quercetin wasn’t detected in any Sambucus nigra cultivars. The most amount of quercetin was determined in wild sambucus nigra, 0.24 mg/g. The most amount of rutin was found in Sambucus nigra Albida, 2.33 mg/g.

This thesis deals with chemical composition of the juice from selected species of elderberry (Sambucus nigra L.). The theoretical part is dedicated to botanical characteristic of elderberry including the origin and the extension. There are also mentioned the medical effects of elderberry with the description of the cultivation of this herb with the examples of particular cultivated species. In the experimental section were laid down the particular chemical parameters (yield, pH, relative density, titrable acidity, formol number, content of instant dry mass and reducing sugar) in the juice from the fruits of these cultivated species of elderberry: Albida, Allesö, Aurea, Bohatka, Dana, Heidegg 13, Haschberg, Körsör, Mammut, Pregarten, Riese aus Voßloch, Sambo, Sambu, Samdal, Sampo, Samyl a Weihenstephan. The median of the yield has been 700,0 ml/kg, the median for the pH assessment has been 4,03. The median of relative density of all species was 1,0361, the median of titrable acidity was 141,83 mmol l -1. The median of the overall content of amino acids was 30,5 ml 0,1 mol l-1 NaOH/100ml, the median of the content of the dry mass was 9,5 weight %. The median of the assessment of the reducing sugar was 5,02%.

The theoretical part of the thesis deals with the technology of fruit juices production and characteristics of the known varieties of elderberry. There are also summarized available information on the presence and properties of minerals in food. In the practical part of the thesis 17 different varieties of elderberry juice and commercially available juice sold as food supplements were analyzed using ICP-MS technique. The concentrations of 15 selected essential and toxic elements were determined in these juices.

The aim of this diploma thesis was to determine the aroma active compounds in different varieties of black elderberry (Sambucus nigra L.) and in elder juices that were mixed in different proportions with grape juice. The sensory analysis of these juices was subsequently performed with focusing on their tastiness. The theoretical part deals with the description of the black elderberry, its occurrence, composition and use at home and in industrial processing, as well as a description of aroma active compounds, which have been previously identified in elder by various authors. In the experimental part aroma active substances were identified and quantified in seven selected varieties of elder. Solid Phase Microextraction method was used for their isolation and subsequent analysis was performed by gas chromatography. 39 aroma active compounds were identified totally in fruits of the black elderberry, of which 15 alcohols, 11 aldehydes, 7 ketones, 5 esters and 1 acid. 45 aroma active compounds were identified totally in elderberry juices, of which 19 alcohols, 8 aldehydes, 5 ketones, 7 esters and 6 acids. The sensory analysis of elder juices was performed using ranking test and evaluation using the scales.

Master´s thesis is focused on cluster analysis. Clustering has its roots in many areas, including data mining, statistics, biology and machine learning. The aim of this thesis is to elaborate a recherche of cluster analysis methods, methods for determining number of clusters and a short survey of feature selection methods for unsupervised learning. The very important part of this thesis is software realization for comparing different cluster analysis methods focused on finding optimal number of clusters and sorting data points into correct classes. The program also consists of feature selection HFS method implementation. Experimental methods validation was processed in Matlab environment. The end of master´s thesis compares success of clustering methods using data with known output classes and assesses contribution of feature selection HFS method for unsupervised learning for quality of cluster analysis.

My diploma thesis deals with the optimization and verification of conditions for separation of proteins, which are contained in the twigs of elder (Sambucus nigra L.) using the technique RP-HPLC. The measurements were made with the use of a HPLC system with a UV-VIS detector. Column Zorbax 300SB-C18 300 4,6 x 250 mm and particles the size of 5 microns were used. The theoretical part describes the attributes and usage of elder and especially its proteins. The basic characteristic of the used HPLC technique is introduced. The possibilities of how to identify proteins are described. The practical part demonstrates the individual steps of optimization of the HPLC method applied to the mixture of standard proteins. The application of this method in the real sample is also reported. The final part of the diploma thesis is focused on the comparison of analysed samples taken in different seasons.

The presented diploma work brings the theory of noise music with the main reason to clarify the pragmatics of its forming and effects. The base of the theory in construct ob the ideas of Antonin Artaud, their reflections and externalizations in the post-structuralist philosophical discourse and intermedial fine arts. Because of the topic, work also observes trends in artictic comunication trough the noise. The aim of work is to formulates the own theory of noise music trough the postulation of cruelty in the artistic speech with emphasis on the explanation of transgresive experience during the noise music performance. The results of the work contributes to the theory of appropriation trough the description of specific medium transmission in the immanent space.

In this thesis was determinated selected chemical and nutritional parameters in the wild elderberry and 17 cultivated varieties of elderberry. The fruits was provided by the Research and Breeding Institute of Pomology Holovousy Ltd. The theoretical part deals describes elderberry, chemical composition of elderberry, especially biological active substances and its using not only food industry. Closer attention has been focused on effect of saccharides, anthocyanins and methods of their determination, specially determination by HPLC. The experimental part describes the various methods of determination of selected chemical and nutritional parameters. Total dry matter, content of total polyphenolic compounds, monomeric anthocyanin pigment and antioxidant activity was defined in all varieties of elderberry fruits. For some varieties was defined total soluble dry matter, content of organic acids and saccharides by various methods. In the end was compared the values from different methods varieties.

The diploma thesis is focused on the problem of homelessness, social work with homeless people, the ethics of care and its usefulness within social work with the homeless. Every particular topic has been described and evaluated from the point of view of various authors, both Czech and foreign. The main part of this diploma thesis is the interconnection of the ethics of care in social work, specifically in social work with the homeless. The ethics of care is then applied into social services which are provided to the homeless. The ethics of care has been also talked about in the context of the organisations which provide help for the homeless. The substantial part of the diploma thesis is a question whether or not the ethics of care in social work with the homeless can be used under the conditions we have in the Czech Republic. Not only the state of social work in the Czech Republic, but also Czech legislation and work of the organisations providing help for the homeless are very important matters to think of. Evaluation is based on the use of all the resources and the aim is to interconnect all the parts of this diploma thesis.

The thesis contains two parts, to bind: The first part describes the phenomenon of homelessness and homeless people in the Czech Republic, with main reference to their ambiguous definition. Characterizes the dedicated assistance to the homeless and from social work to therapeutic help. Describes the basic form of therapeutic exposure and focus, emphasizes psychotherapeutic orientations, which are suitable for a group of homeless people, with an emphasis on cognitive-behavioral therapy (CBT). The first part of the research continues, describing the organization of the St. Paul's House (DsP), which took place in the first orientation research whose aim was to determine whether CBT is the most appropriate psychotherapeutic direction in psychotherapeutic work with the homeless in the DsP, and how psychotherapy is implemented in the DsP. After the landmark research was carried out research whose aim was to find feedback, experience and opinion shelter users in the implementation of the DsP and the course of psychotherapy.