In the summer of 2010, fieldwork was conducted in the Robertson Valley, Canadian Rocky Mountains, Alberta to assess the magnitude and controls of microbial nitrification in proglacial till and in supraglacial, subglacial, and proglacial streams. Seasonal precipitation and glacial and proglacial runoff was sampled for hydrochemical and stable isotope analyses (δ18O and δ15N of nitrate [NO3-]). Lower Ca:Mg ratios, higher mean Σmajor ions, and an increased importance of reactions with slower dissolution kinetics in subglacial streams and proglacial seeps indicated waters here experienced longer rock-water contact time than in dilute supraglacial streams. Additionally, waters emanating from longer residence time flowpaths acquired substantial NO3- from nitrification reactions. Using δ18O-NO3- in a simple end-member mixing model, the fraction of NO3- derived from microbial nitrification was estimated to be 44 to 56% in the two subglacial streams, and greater than 80% in proglacial seeps. These results show that atmospherically-derived nitrogen (N) in this glacial valley undergoes substantial biological cycling prior to export in surface runoff. Water flowing from the east subglacial stream (RE) received a larger portion of its melt from a sediment-rich, slow drainage system and had a higher proportion of nitrified NO3- compared to the west subglacial stream (RW), where runoff was similar in composition to supraglacial runoff, indicating that the nature of subglacial flowpaths is an important factor in determining the amount of microbially-cycled nutrients that are exported from a glacier. Sixteen 34-day in situ soil incubations revealed that net mineralization and net nitrification occurred at all four sampling sites in the glacier forefield along a 1.6 km chronosequence; however, there was no significant difference among these rates with time since deglaciation or temperature. Instead, net mineralization and net nitrification rates were significantly correlated (p < 0.05, n = 16) with measured physical and chemical soil variables, including total organic carbon, total N, bulk density, pH, and clay content, suggesting that substrate availability is a larger control on N-cycling processes than time since deglaciation. High variability in inorganic soil N pools and N-cycling rates indicates that there are likely hot spots of biogeochemical activity within glacial till. / Thesis (Master, Geography) -- Queen's University, 2012-04-26 14:47:17.29

In an upland forest in the Experimental Lakes Area (ELA), Ontario, dissolved organic carbon (DOC) concentrations in precipitation increased with passage through a forest canopy, as throughfall and stemflow. A maximum mean concentration of 67 mg/l occurred in the forest floor, which decreased to 11 mg/l in the B horizon, as DOC was sequestered in the soil. High DOC concentrations were measured in an ephemeral stream draining the upland, as a result of saturated overland flow, and the leaching of litter and woody material. / Porewater DOC concentrations decreased with depth in two bogs. The porewater DOC was comprised primarily of acidic fractions (70 to 87%), with a predominance of hydrophobic acids. The bogs were significant sources of DOC, exporting between 17.2 and 29.4 g DOC/m$ sp2$ over the study period. In 1993, a bog was flooded simulating the creation of a hydroelectric reservoir, and resulting in significant increases in porewater DOC concentrations at the surface. A maximum surface concentration of 223 mg/l was measured in late September. The flooding of the bog also resulted in significant increases in DOC concentrations in the draining waters, with the outflow of the watershed increasing from a mean of 20.5 mg/l in 1992, to 14.7 mg/l in 1993.

In order to better quantify the continuous net ecosystem exchange (NEE) at the farm scale, a 24 m high transportable tower-based eddy covariance and profiling system was tested during three field campaigns during the summer and autumn of 2006. The profile measurement system, equipped with a low-cost closed-path infrared gas analyser (LI-840) was designed to measure the carbon dioxide (CO2) storage especially during weak mixing periods under stable conditions. A tethered blimp-based measurement system for the nocturnal boundary layer (NBL) budget technique was deployed on several calm nights to compare with the concentrations and fluxes determined with the profiling system. The profiling system was also installed to determine horizontal and vertical advection. The two-dimensional horizontal positioning of the profile inlets allowed an examination of the CO2 advective gradient in relation to the wind direction. A near-source advection analysis provided parameters for further adjustments of the NEE at the actual farmland.

Iron is recognized as a key element regulating primary production in large regions of the ocean, but nothing is known of its direct effect on higher trophic levels. Growth and metabolism of two species of heterotrophic protozoans fed iron-rich and iron-poor prey were thus examined. Maximum growth rates of Paraphysomonas imperforata and P. butcheri were observed only when Fe quotas of bacterial prey were greater than 70 $ mu$mol Fe:mol C. At lower Fe:C ratios, but at constant prey biomass (C/ml), both species grew significantly slower. Minimum Fe quotas of the flagellates at these slow growth rates ($ sim$10 $ mu$mol Fe:mol C) were similar to those of iron-limited phytoplankton and bacteria. Growth rate reduction was the result of direct elemental limitation by Fe, judging from the protozoans' positive response to Fe additions and from their biochemical characteristics. Filtration and carbon ingestion rates increased under Fe-limitation, but carbon gross growth efficiency (CGGE) decreased when Paraphysomonas imperforata consumed iron-poor bacteria. Ammonium regeneration efficiency was also reduced. The decrease in CGGE was a consequence of reduced activity of the iron-dependent electron transport system, greater DOC excretion, and greater CO$ sb2$ evolution by Fe-limited flagellates. Paraphysomonas imperforata excreted Fe, even when limited by this element, and retained less of the ingested ration and thus had a higher Fe regeneration efficiency than when consuming Fe-rich bacteria. According to recent measurements of biogenic Fe:C in the subarctic Pacific, our results suggest that heterotrophic bacterivorous flagellates may experience iron-limitation in remote oceanic regions. Such limitation could profoundly affect C, N and Fe cycling in the sea.

This study examines carbon flow in the Dipper Harbour salt marsh, a macrotidal system located on the north-west coast of the Bay of Fundy, New Brunswick. The vegetated marsh surface is composed of three major zones; the Spartina alterniflora-dominated low marsh, the Plantago maritima-dominated Middle marsh, and the Spartina patens-dominated high marsh. The total net primary production (NPP) of these dominant macrophytes is 860, 300 and 650 g C m$ sp{-2}$ yr$ sp{-1}$ respectively. In all plant zones, 66% of the NPP occurs in the belowground fraction. / Empirical measurements of organic matter burial indicate that the marsh sediment acts as a carbon sink, accumulating between 75 and 105 g C m$ sp{-2}$ yr$ sp{-1}$. The tidal export of aboveground plant biomass in the form of particulate organic matter accounts for a net loss of carbon ranging from 65 to 170 g C m$ sp{-2}$ yr$ sp{-1}$. An experiment examining the exchange of dissolved organic carbon (DOC) suggest a net annual export of roughly 500 g C m$ sp{-2}$ yr$ sp{-1}$. The forementioned fluxes are combined with estimates of surface gas exchange and algal productivity in order to construct a carbon budget. The budget predicts a DOC export term of 365 g C m$ sp{-2}$ yr$ sp{-1}$, which is of the same order of magnitude as that obtained from the empirical DOC data. / The results of this study show that the Plantago zone plays a significant role in the circulation of carbon in the Dipper Harbour salt marsh. This suggests that the patterns of carbon circulation in northern marshes may differ considerably from those in marshes further south where no distinct Plantago zone has been reported.

The sources, sinks, fluxes, spatial distributions, and temporal variations of dissolved organic carbon (DOC) in subarctic fen catchments as well as the temporal patterns of DOC in streams draining subarctic fen catchments in the region of Schefferville, Quebec were investigated. / In June to August sampling, DOC concentrations averaged 17 mg/L in peat water, 2-16 m/L in stream water, 49-56 mg/L in canopy throughfall, 14-19 mg/L in understory throughfall, 122-187 mg/L in stemflow, 25-39 mg/L in lichen and moss mat water, and 35-42 mg/L in soil A horizon water. / Precipitation and canopy and understory throughfall were all significant DOC sources with seasonal DOC fluxes to the forest floor of 0.1-0.4, 0.5-1.3, and 0.8-1.7 g DOC/m$ sp2$ of forest, respectively. The lichen and moss mats and the A soil horizon were also found to be DOC sources, whereas the B soil horizon was a DOC sink. The soil column was estimated to export 0.4-0.5 g DOC/m$ sp2$. Peat, also a DOC source, released 1.2-2.1 g DOC/m$ sp2$. / DOC concentrations in streams draining ten fen catchments were found to be positively correlated with the percentage of fen area in the catchments.

Energy availability and environmental issues are of growing concern; nations are striving to use energy more efficiently while at the same time decreasing the negative impacts on the environment. / The objectives of this study are to: (1) establish a supply price for ethanol derived from switchgrass, (2) establish an accounting budget for carbon dioxide during feedstock production and processing into ethanol, and (3) determine the cost/tonne of CO$ sb2$ using this strategy. / Total cost of production of the feedstock for cycles of 5, 10, 15, and 20 years are $357.06/ha ( $32.73/ODT), $337.81/ha ( $30.96/ODT), $331.52/ha ( $30.39/ODT), and $328.47/ha ( $30.11/ODT) respectively. Thus, the approximate cost of producing one litre of ethanol from switchgrass is $0.47/litre. / Overall, the switchgrass-ethanol system is a net carbon sink for all four cycles, and each hectare of swithgrass sequesters between 1.9 and 6.8 tonne of CO$ sb2$ per year. However, this process is not indefinite, and will stop once the soil organic matter reaches an equilibrium. / The current price for gas is $0.24/litre (excluding taxes and profit margins). According to the price differential existing between ethanol and gasoline and the CO$ sb2$ emissions difference between both systems, the cost of sequestering CO$ sb2$ ranges between $83/tonne and $129/tonne, with the adoption of this specific strategy. At the macro level, Quebec and Canada's total carbon dioxide emissions reached 70 million tonnes and 461 million tonnes respectively, in 1991. Thus, displacing fossil-based energy sources (gasoline) with a renewable energy source (ethanol from switchgrass) to reduce those emissions by 20 % by the year 2010, incurs a cost ranging between $1.16 billion and $1.8 billion for Quebec, and between $7.7 billion and $11.9 billion for Canada. (Abstract shortened by UMI.)

The overall objective of the research presented here was to combine multiple geochemical parameters and molecular characterizations to provide a novel view of active microbial community ecology of sediments in a large-river deltaic estuary. In coastal and estuarine environments, a large portion of benthic respiration has been attributed to sulfate reduction and implicated as an important mechanism in hypoxia formation. The use of high-resolution sampling of individual sediment cores and high throughput nucleic acid extraction techniques combined with 454 FLX sequencing provided a robust understanding of the metabolically active benthic microbial community within coastal sediments. This was used to provide further understanding and show the importance of simultaneously analyzing the connectivity of sulfur and iron cycling to the structure and function of the microbial population. Although aqueous sulfide did not accumulate in the sediments of the northern Gulf of Mexico, active sulfate reduction was observed in all locations sampled. Microbial recycling and sequestration as iron sulfides prevented the release of sulfide from the sediment. Prominent differences were observed between the sample locations and with depth into the sediment column. This study emphasized the importance of combining novel molecular techniques with simultaneous traditional geochemical measurements to show the interdependence of microbiology and geochemistry. In addition, this study highlights the need to consider microbial community biogeography along with small-scale variations in geochemistry and biology that impact the overall cycling of redox elements when constructing biogeochemical models in marine sediments.

This thesis investigates how benthic fluxes of phosphorus (P), nitrogen (N), and silicon (Si) change upon oxygenation of anoxic soft bottoms in the brackish, eutrophicated Baltic Sea. Direct measurements in situ by benthic landers demonstrated that fluxes of dissolved inorganic P (DIP) from anoxic bottom sediments in the Eastern Gotland Basin are higher than previously thought (Paper I). It is argued that the benthic DIP flux has a much larger influence on the DIP inventory in the Baltic proper than the external sources. Similarly, benthic fluxes of DIP and dissolved inorganic N (DIN) from anoxic sediment in the coastal Kanholmsfjärden Basin, Stockholm archipelago, were sufficiently high to renew the pools of these nutrients below the upper mixed layer in roughly one year (Paper II). A natural inflow of oxygen rich water into the deep, and previously long-term anoxic part of Kanholmsfjärden Basin, increased the P content in the sediment by 65% and lowered DIP and dissolved silica (DSi) concentrations in the pore water. These changes, as well as the large increases in benthic effluxes of these solutes following de-oxygenation of the bottom water, suggest that they are influenced similarly by changing oxygen conditions. Experimental results in papers III and IV show that common benthic macrofauna species in the Baltic Sea can stimulate benthic release of DIN and DSi, as well as dissolved organic and particulate bound nutrients. Thus, if benthic oxygen conditions would improve in the Baltic, initial effects on benthic–pelagic nutrient coupling will change due to animal colonisation of currently azoic soft bottoms. A new box corer was designed (Paper V) which can be used to obtain highly needed virtually undisturbed samples from soft bottom sediments – if lowered slowly and straight into the bottom strata – as demonstrated by in situ videography and turbidimetry. The commonly used USNEL box corer caused severe biasing during sediment collection. / <p>At the time of the doctoral defense, the following papers were unpublished and had a status as follows: Paper 2: Manuscript. Paper 4: Manuscript. Paper 5: Manuscript.</p> / Baltic oxygenation project

The western Antarctic Peninsula (WAP) is a hotspot of climatic and oceanographic change, with a 6°C rise in winter atmospheric temperatures and >1°C warming of the surface ocean since the 1950s. These trends are having a profound impact on the physical environment at the WAP, with widespread glacial retreat, a 40% decline in sea ice coverage and intensification of deep water upwelling. The main objective of this study is to assess the response of phytoplankton productivity to these changes, and implications for the marine carbon and nitrogen cycles in the WAP coastal zone. An extensive suite of biogeochemical and physical oceanographic data was collected over five austral summer growing seasons in northern Marguerite Bay between 2004 and 2010. Concentrations and isotopic compositions ( 15N, 13C, 14C) of dissolved nitrate, dissolved inorganic carbon species, particulate nitrogen, organic carbon and chlorophyll a are used in the context of a substantial ancillary dataset to investigate nutrient supply, phytoplankton productivity and nutrient uptake, export flux and the fate of organic material, and the factors underpinning pronounced seasonal and interannual variability. High-resolution biogeochemical time-series data for surface and underlying seawater, sea ice brine, sediment trap material and coretop sediments allow detailed examination of carbon and nitrogen cycle processes under contrasting oceanographic conditions and the interaction between these marine processes and air-sea exchange of climate-relevant CO2. This study shows that the WAP marine environment is currently a summertime sink for atmospheric CO2 in most years due to high productivity and biological carbon uptake sufficient to offset the CO2 supply from circumpolar deep waters, which act as a persistent source of heat, nutrients and CO2 across the shelf. For the first time, CO2 sink/source behaviour is parameterised in terms of nitrate utilisation, by exploiting the relationship between CO2 and nitrate concentrations, and deriving the nitrate depletion at which surface ocean CO2 is undersaturated relative to atmosphere and carbon sink behaviour is achieved. This could have vast utility in examining CO2 sink/source dynamics over greater spatial and temporal scales than by direct CO2 measurements, of which availability is more limited. This study documents abrupt changes in phytoplankton productivity, nitrate utilisation and biological CO2 uptake during a period of rapid sea ice decline. In fact, nitrate utilisation, particulate organic matter production and biological CO2 uptake all decrease by at least 50 % between a sea ice-influenced, high productivity season and one of low sea ice and low productivity. The key driver of interannual variability in production and export of organic material is found to be upper ocean stratification and its regulation of light availability to phytoplankton. Productivity, CO2 uptake and export are maximal when stratification is sufficient to provide a stable well-lit surface environment for phytoplankton growth, but with some degree of mixing to promote export of suspended organic matter. Strong stratification causes intense initial production, but retention of suspended organic particles in the surface ocean induces a self-shading effect, and overall productivity, CO2 uptake and export fluxes are low. When stratification is weak, mixing of phytoplankton over a larger depth range exposes cells to a wider range of light levels and reduces photosynthetic efficiency, thus total productivity and CO2 uptake. A conceptual model is developed here, which attempts to describe the mechanism by which sea ice dynamics exert the principal control on stratification and therefore productivity and CO2 uptake at the WAP, with potential application to other regions of the Antarctic continental shelf. Although meteoric waters (glacial melt and precipitation) are more prevalent in surface waters throughout the study, sea ice meltwater variability is driven by large and rapid spring/early summer pulses, which stabilise the upper ocean and initiate phytoplankton growth. The timing and magnitude of these sea ice melt pulses then exert the key control on stratification and seasonal productivity. In a low sea ice year of this study, the sea ice trigger mechanism was absent and productivity was low. This strongly suggests that ongoing sea ice decline at the WAP and greater frequency of such low sea ice years is likely to drive a dramatic reduction in productivity and export, which would substantially reduce the capacity of the summertime CO2 sink in this region. Ongoing warming and ecosystem change are thus likely to have severe impacts on net CO2 sink/source behaviour at the WAP over the annual cycle, and the role of the Southern Ocean in regulating atmospheric CO2 and global climate. Finally, factors influencing the stable isotopic signature of particulate organic carbon ( 13CPOC), a common paleo-proxy, are assessed. 13CPOC is greatly influenced by seasonal shifts in diatom assemblages and isotopically heavy sea ice material, so cannot be used as a robust proxy for ambient CO2 in the coastal Southern Ocean.