Advances in molecular immunology and rapid technical evolution during the past two decades have led to a new class of medicines called biologics. Recently, a large number of biologics, or biologic immunomodulators, directed towards an array of immune–mediated diseases, have entered the market. This has lead to a dramatic change in the immunotherapy of autoimmune diseases, as biologics present new potential to improve or substitute conventional immunosuppressive therapies. According to literature, biologics are used by only a small number of a health plan’s members, (approximately one per cent), but a single occurrence can be relatively expensive. Furthermore, there is an indication that the frequency of use and cost of biologics are on the rise, and as more biologics enter the market, health plans and employers face the challenge of controlling costs while ensuring that biologics are affordable. The general objective of this study was to determine the prevalence and cost of biologic immunomodulating medicine in the treatment of certain autoimmune diseases during the period 2005 to 2008 in a section of the private health care sector of South Africa, by employing a medicine claims database as a source to obtain necessary information. A quantitative, retrospective drug utilisation review (rDUR) was performed on computerised medication records (medicine claims data) for four consecutive years (i.e. 2005 to 2008) provided by a pharmacy benefit management company (PBM). The study population consisted of all patients on the database who received at least one medicine item with adalimumab, etanercept, infliximab, interferon beta–1a, interferon 1–b or rituximab as active ingredient and who were diagnosed with either rheumatoid arthritis (RA), multiple sclerosis (MS) or Crohn’s disease between 1 January 2005 and 31 December 2008. Between 2005 and 2008, an average of 1,305,201 patients appeared on the total database, and of these 0.055% (n = 713) received biologic immunomodulating medicine. More than two thirds of biological users were female and most patients who received these medicine items were between the ages of 39 and 64 years, followed by those patients aged between 25 and 39 years. Biologic immunomodulating medicine items (n = 11,914) and biologic prescriptions (n = 9,537) represented 0.016% of the total number of medicine items (N = 76,129,173) and 0.030% of the total number of prescriptions (N = 31,985,153). The percentage contribution of biologic immunomodulators to the total number of medicine items and prescriptions on the total database increased each year, and in four years’ time the percentage of all the medicine items on the total database that included biologic immunomodulators had tripled, from 0.009% to 0.023%. The total cost of biologic immunomodulating medicine accounted for 1.278% of the total cost (N = R7, 483,759,176.23) of all medication claimed through the PBM between 2005 and 2008. The percentage contribution of biologic immunomodulators to the total medicine expenditure also increased from one year to another for the four–year study period. The average cost of a biologic immunomodulating medicine item increased with 71.10% from 2005 (R5602.71 ± 2166.61) to (R9586.25 ± 5956.56) in 2008. The CPI for biologic immunomodulators, (CPI = 60.00 for 2005; CPI = 74.62.17 for 2006; CPI = 85.26 for 2007; and CPI = 86.96 for 2008) indicated that biologic immunomodulating medicine items were relatively expensive and the d–value between the average cost per biologic immunomodulator and the average cost per non–biological medicine item (d–value = 2.54 in 2005, d–value = 3.32 in 2006, d–value = 2.23 in 2007 and d–value = 1.59 in 2008) furthermore indicated that the impact of biological therapies was large and practically significant. Rheumatoid arthritis patients represented 19.78% of the total number of patients (n = 713) who claimed the biologic immunomodulators during the four–year period, MS patients (n = 172) represented 24.12% and Crohn’s patients (n = 11) represented 1.5%. Biological drugs prescribed to RA patients represented 0.28% (n = R20, 708,818.82) of the total cost (N = R7, 483,759,176.23) of all medication claimed through the PBM during the four–year period, while those prescribed to MS patients represented 0.41% (R30, 922,520.07) and those prescribed to Crohn’s disease patients represented 0.015% (R1, 108,568.02). Although biologic immunomodulating medicine items used in the treatment of RA, MS and Crohn’s disease are relatively expensive, it seems that the number of other medication prescribed to patients with these diseases decreased after treatment with biologics, which may influence the medicine treatment cost of these patients. It can be concluded that even though biologic immunomodulators are used by only a very small percentage of the total patient population in a section of the private health care sector of South Africa, they are relatively expensive and have a considerable impact not only the medical aid scheme, but also on the patient. / Thesis (M.Pharm (Pharmacy Practice))--North-West University, Potchefstroom Campus, 2011.

Advances in molecular immunology and rapid technical evolution during the past two decades have led to a new class of medicines called biologics. Recently, a large number of biologics, or biologic immunomodulators, directed towards an array of immune–mediated diseases, have entered the market. This has lead to a dramatic change in the immunotherapy of autoimmune diseases, as biologics present new potential to improve or substitute conventional immunosuppressive therapies. According to literature, biologics are used by only a small number of a health plan’s members, (approximately one per cent), but a single occurrence can be relatively expensive. Furthermore, there is an indication that the frequency of use and cost of biologics are on the rise, and as more biologics enter the market, health plans and employers face the challenge of controlling costs while ensuring that biologics are affordable. The general objective of this study was to determine the prevalence and cost of biologic immunomodulating medicine in the treatment of certain autoimmune diseases during the period 2005 to 2008 in a section of the private health care sector of South Africa, by employing a medicine claims database as a source to obtain necessary information. A quantitative, retrospective drug utilisation review (rDUR) was performed on computerised medication records (medicine claims data) for four consecutive years (i.e. 2005 to 2008) provided by a pharmacy benefit management company (PBM). The study population consisted of all patients on the database who received at least one medicine item with adalimumab, etanercept, infliximab, interferon beta–1a, interferon 1–b or rituximab as active ingredient and who were diagnosed with either rheumatoid arthritis (RA), multiple sclerosis (MS) or Crohn’s disease between 1 January 2005 and 31 December 2008. Between 2005 and 2008, an average of 1,305,201 patients appeared on the total database, and of these 0.055% (n = 713) received biologic immunomodulating medicine. More than two thirds of biological users were female and most patients who received these medicine items were between the ages of 39 and 64 years, followed by those patients aged between 25 and 39 years. Biologic immunomodulating medicine items (n = 11,914) and biologic prescriptions (n = 9,537) represented 0.016% of the total number of medicine items (N = 76,129,173) and 0.030% of the total number of prescriptions (N = 31,985,153). The percentage contribution of biologic immunomodulators to the total number of medicine items and prescriptions on the total database increased each year, and in four years’ time the percentage of all the medicine items on the total database that included biologic immunomodulators had tripled, from 0.009% to 0.023%. The total cost of biologic immunomodulating medicine accounted for 1.278% of the total cost (N = R7, 483,759,176.23) of all medication claimed through the PBM between 2005 and 2008. The percentage contribution of biologic immunomodulators to the total medicine expenditure also increased from one year to another for the four–year study period. The average cost of a biologic immunomodulating medicine item increased with 71.10% from 2005 (R5602.71 ± 2166.61) to (R9586.25 ± 5956.56) in 2008. The CPI for biologic immunomodulators, (CPI = 60.00 for 2005; CPI = 74.62.17 for 2006; CPI = 85.26 for 2007; and CPI = 86.96 for 2008) indicated that biologic immunomodulating medicine items were relatively expensive and the d–value between the average cost per biologic immunomodulator and the average cost per non–biological medicine item (d–value = 2.54 in 2005, d–value = 3.32 in 2006, d–value = 2.23 in 2007 and d–value = 1.59 in 2008) furthermore indicated that the impact of biological therapies was large and practically significant. Rheumatoid arthritis patients represented 19.78% of the total number of patients (n = 713) who claimed the biologic immunomodulators during the four–year period, MS patients (n = 172) represented 24.12% and Crohn’s patients (n = 11) represented 1.5%. Biological drugs prescribed to RA patients represented 0.28% (n = R20, 708,818.82) of the total cost (N = R7, 483,759,176.23) of all medication claimed through the PBM during the four–year period, while those prescribed to MS patients represented 0.41% (R30, 922,520.07) and those prescribed to Crohn’s disease patients represented 0.015% (R1, 108,568.02). Although biologic immunomodulating medicine items used in the treatment of RA, MS and Crohn’s disease are relatively expensive, it seems that the number of other medication prescribed to patients with these diseases decreased after treatment with biologics, which may influence the medicine treatment cost of these patients. It can be concluded that even though biologic immunomodulators are used by only a very small percentage of the total patient population in a section of the private health care sector of South Africa, they are relatively expensive and have a considerable impact not only the medical aid scheme, but also on the patient. / Thesis (M.Pharm (Pharmacy Practice))--North-West University, Potchefstroom Campus, 2011.

Gold mine tailings material facilities are characterized by sparse vegetation and an abundance of dust. Mine tailings facilities are examples of extreme geotechnical and geochemical conditions which make it almost impossible for higher plants to establish and grow without rehabilitation intervention. In most cases higher plants such as grasses and trees are the focus areas for rehabilitation, but, having a look at something a little smaller such as biological crusts, it is seen that these micro-organisms play very important roles in any ecosystem. Various studies have shown that biological crusts, consisting of micro-organisms such as lichens, algae and cyanoprokaryotes enhance the soil quality by binding soil particles together, forming aggregates which counteract the erosive forces of wind and water. They play a part in nitrogen and carbon fixation, increase the soil surface temperature and increase the water retention of the soil. Thus, these organisms improve the overall health of the soil, which will in time encourage the successful establishment of higher plants. The aim of this study was to investigate the presence of cyanoprokaryotes and soil algae on mine tailings storage facilities that have been rehabilitated for different periods of time as well as to correlate the presence of these species with the physical and chemical characteristics of the mine tailings material. Chemical, physical and biological analyses of soil samples were done. Some of the ecologically important and dominant species were isolated and protocols were developed in order to identify the most successful manner in which to re-inoculate the organisms to a chosen substrate and how to measure biomass. Due to the immense cost of standard rehabilitation practices there is a need for a more cost effective, sustainable manner in which to protect the tailings material against the erosive forces of wind and water with as little input as possible. The influence of an organism cultured in normal Bold’s Basal medium (BBM) growth medium, BBM growth medium with half the phosphate concentration and BBM growth medium with half the nitrate concentration on the establishment of a biological soil crust (BSC) was tested. To test the influence of the inoculums already present in the tailings material and in the air, trials with mulch, water and nutrients without the addition of an organism was also investigated. This was done in the controlled environment of a glasshouse, as well as in field conditions. The biomass of the cyanoprokaryotes and algae, as well as the soil surface strength was also tested. The results show that the time of rehabilitation did not have an influence on the cyanoprokaryotes as well as algal species that occurred on the tailings material. Chlorella sp., Chlorococcum sp. and Klebsormidium sp. were present on all six sites, except on the fresh material and 15 year old material where no rehabilitation has been done. As for dominance; Chlamydomonas sp., Chlorococcum sp., Klebsormidium sp. and Phormidium sp. were dominant on all six sites except for the fresh material, where nothing grew. An array of methods exists for measuring algal biomass as a measure of growth. During the development of protocols for further use in investigating the growth of algae, the extraction solvent ethanol, for use in chlorophyll a extraction, was identified as the most sufficient. The re-inoculation of cyanoprokaryotes and soil algae onto a chosen substrate is most successful when pouring the organisms, cultured in growth medium and 0.1% agar, over the substrate. During the glasshouse trials the influence of the growth medium and growth medium with half the nitrate and half the phosphate concentrations showed that Chlamydomonas sp. produced the highest biomass when cultured in BBM. With Nostoc sp. the highest biomass occurred with culturing in BBM and BBM with half the phosphate concentration. Microcoleus vaginatus showed no significant difference when cultured in the three different growth mediums (BBM, BBM with half the nitrate concentration and BBM with half the phosphate concentration). Overall Nostoc sp. produced the highest biomass (34.33 μg/g), followed by Microcoleus vaginatus (17.05 μg/g) and Chlamydomonas sp. (6.12 μg/g). Soil surface strength, measured with a hand held penetrometer showed that Chlamydomonas sp. cultured in BBM growth medium produced the most stable crust (2.58 kg/cm2), although it had the lowest biomass measurements (6.12 μg/g). Nostoc sp. produced the highest biomass (34.44 μg/g), but had the lowest soil surface strength results (1.75 kg/cm2). Microcoleus vaginatus proved to be the species with high biomass production (17.05 μg/g), as well as high soil surface strength (2.08 kg/cm2). M. vaginatus is also a pioneer species and is therefore a good choice as primary inoculum on bare tailings material. It was decided to use Nostoc sp. in the field trials due to its high biomass and Microcoleus vaginatus due to the high soil surface strength produced. Despite the occurrence of a severe thunder storm on the afternoon of application and poor water management during the field trials the significance of water on the establishment of soil algae and cyanoprokaryotes on tailings material was determined. / MSc (Environmental Sciences), North-West University, Potchefstroom Campus, 2014

Gold mine tailings material facilities are characterized by sparse vegetation and an abundance of dust. Mine tailings facilities are examples of extreme geotechnical and geochemical conditions which make it almost impossible for higher plants to establish and grow without rehabilitation intervention. In most cases higher plants such as grasses and trees are the focus areas for rehabilitation, but, having a look at something a little smaller such as biological crusts, it is seen that these micro-organisms play very important roles in any ecosystem. Various studies have shown that biological crusts, consisting of micro-organisms such as lichens, algae and cyanoprokaryotes enhance the soil quality by binding soil particles together, forming aggregates which counteract the erosive forces of wind and water. They play a part in nitrogen and carbon fixation, increase the soil surface temperature and increase the water retention of the soil. Thus, these organisms improve the overall health of the soil, which will in time encourage the successful establishment of higher plants. The aim of this study was to investigate the presence of cyanoprokaryotes and soil algae on mine tailings storage facilities that have been rehabilitated for different periods of time as well as to correlate the presence of these species with the physical and chemical characteristics of the mine tailings material. Chemical, physical and biological analyses of soil samples were done. Some of the ecologically important and dominant species were isolated and protocols were developed in order to identify the most successful manner in which to re-inoculate the organisms to a chosen substrate and how to measure biomass. Due to the immense cost of standard rehabilitation practices there is a need for a more cost effective, sustainable manner in which to protect the tailings material against the erosive forces of wind and water with as little input as possible. The influence of an organism cultured in normal Bold’s Basal medium (BBM) growth medium, BBM growth medium with half the phosphate concentration and BBM growth medium with half the nitrate concentration on the establishment of a biological soil crust (BSC) was tested. To test the influence of the inoculums already present in the tailings material and in the air, trials with mulch, water and nutrients without the addition of an organism was also investigated. This was done in the controlled environment of a glasshouse, as well as in field conditions. The biomass of the cyanoprokaryotes and algae, as well as the soil surface strength was also tested. The results show that the time of rehabilitation did not have an influence on the cyanoprokaryotes as well as algal species that occurred on the tailings material. Chlorella sp., Chlorococcum sp. and Klebsormidium sp. were present on all six sites, except on the fresh material and 15 year old material where no rehabilitation has been done. As for dominance; Chlamydomonas sp., Chlorococcum sp., Klebsormidium sp. and Phormidium sp. were dominant on all six sites except for the fresh material, where nothing grew. An array of methods exists for measuring algal biomass as a measure of growth. During the development of protocols for further use in investigating the growth of algae, the extraction solvent ethanol, for use in chlorophyll a extraction, was identified as the most sufficient. The re-inoculation of cyanoprokaryotes and soil algae onto a chosen substrate is most successful when pouring the organisms, cultured in growth medium and 0.1% agar, over the substrate. During the glasshouse trials the influence of the growth medium and growth medium with half the nitrate and half the phosphate concentrations showed that Chlamydomonas sp. produced the highest biomass when cultured in BBM. With Nostoc sp. the highest biomass occurred with culturing in BBM and BBM with half the phosphate concentration. Microcoleus vaginatus showed no significant difference when cultured in the three different growth mediums (BBM, BBM with half the nitrate concentration and BBM with half the phosphate concentration). Overall Nostoc sp. produced the highest biomass (34.33 μg/g), followed by Microcoleus vaginatus (17.05 μg/g) and Chlamydomonas sp. (6.12 μg/g). Soil surface strength, measured with a hand held penetrometer showed that Chlamydomonas sp. cultured in BBM growth medium produced the most stable crust (2.58 kg/cm2), although it had the lowest biomass measurements (6.12 μg/g). Nostoc sp. produced the highest biomass (34.44 μg/g), but had the lowest soil surface strength results (1.75 kg/cm2). Microcoleus vaginatus proved to be the species with high biomass production (17.05 μg/g), as well as high soil surface strength (2.08 kg/cm2). M. vaginatus is also a pioneer species and is therefore a good choice as primary inoculum on bare tailings material. It was decided to use Nostoc sp. in the field trials due to its high biomass and Microcoleus vaginatus due to the high soil surface strength produced. Despite the occurrence of a severe thunder storm on the afternoon of application and poor water management during the field trials the significance of water on the establishment of soil algae and cyanoprokaryotes on tailings material was determined. / MSc (Environmental Sciences), North-West University, Potchefstroom Campus, 2014

Horticulture is a major foreign exchange earner in Kenya and provides employment to approximately 75% of the population. However, the growth of the horticultural industry is constrained by pests such as the leafminer flies, Liriomyza sativae, L. trifolii and L. huidobrensis (Diptera: Agromyzidae). These pests do not only cause damage to crops, but are also tagged as quarantine pests, resulting in export rejections, loss of export markets and consequently loss of revenue to smallholders. The management of leafminers worldwide has commonly relied on the use of chemical insecticides, but due to associated negative effects thereof, biological control using parasitoids and entomopathogenic fungi has been proposed as major components of integrated pest management (IPM) strategies. The indigenous ectoparasitoid Diglyphus isaea and exotic endoparasitoid Phaedrotoma scabriventris are the two key natural enemies being considered. A number of endophytic fungal isolates have been identified with potential for use as biological control agents of pests. Although there have been previous reports on toxicity of fungal endophytes to leafminers, no attempts have been made to exploit them for control of Liriomyza leafminers. The objectives of this study were to investigate the mechanisms by which fungal endophytes control Liriomyza spp., as well as the interactions between these endophytic fungi and the L. huidobrensis, endoparasitoid Phaedrotoma scabriventris and ectoparasitoid Diglyphus isaea. This study showed that under laboratory conditions, while used separately, parasitism rates of L. huidobrensis by D. isaea and P. scabriventris were 63.6 ± 7.7% and 30.4 ± 10.9% respectively and increased to 77.0 ± 5.3% when used simultaneously. In addition, both parasitoids induced leafminer mortality through larval-feeding and stinging. In order to identify and characterize endophytic fungi that could possibly be used for control of these pests, fungi were isolated from the aboveground parts of maize, sorghum, Napier grass, Coleopteran larvae and Busseola fusca pupae. Identified fungi were evaluated endophytically in two host plants species (Phaseolus vulgaris and Vicia faba) through seed inoculation. The fungal isolates that succeeded in colonizing the host plants were all pathogenic to L. huidobrensis, causing 100% mortality within 13.2 ± 0.7-15.0 ± 0.6 days. They were also able to reduce the longevity of the progeny, the number of pupae and adult emergence and survival. In addition, results also showed that endophytically-inoculated and L. huidobrensis-infested V. faba plants had no adverse effects on parasitism rates and life history parameters of P. scabriventris and D. isaea. Bio-prospecting for fungal endophytes in P. vulgaris and V. faba seeds, followed by morphological and molecular identification revealed the presence of various species of fungal entomopathogens, including Beauveria bassiana, Epacris microphylla, Phanerochaete chrysosporium and Metarhizium anisopliae. / PhD (Environmental Sciences), North-West University, Potchefstroom Campus, 2013

Horticulture is a major foreign exchange earner in Kenya and provides employment to approximately 75% of the population. However, the growth of the horticultural industry is constrained by pests such as the leafminer flies, Liriomyza sativae, L. trifolii and L. huidobrensis (Diptera: Agromyzidae). These pests do not only cause damage to crops, but are also tagged as quarantine pests, resulting in export rejections, loss of export markets and consequently loss of revenue to smallholders. The management of leafminers worldwide has commonly relied on the use of chemical insecticides, but due to associated negative effects thereof, biological control using parasitoids and entomopathogenic fungi has been proposed as major components of integrated pest management (IPM) strategies. The indigenous ectoparasitoid Diglyphus isaea and exotic endoparasitoid Phaedrotoma scabriventris are the two key natural enemies being considered. A number of endophytic fungal isolates have been identified with potential for use as biological control agents of pests. Although there have been previous reports on toxicity of fungal endophytes to leafminers, no attempts have been made to exploit them for control of Liriomyza leafminers. The objectives of this study were to investigate the mechanisms by which fungal endophytes control Liriomyza spp., as well as the interactions between these endophytic fungi and the L. huidobrensis, endoparasitoid Phaedrotoma scabriventris and ectoparasitoid Diglyphus isaea. This study showed that under laboratory conditions, while used separately, parasitism rates of L. huidobrensis by D. isaea and P. scabriventris were 63.6 ± 7.7% and 30.4 ± 10.9% respectively and increased to 77.0 ± 5.3% when used simultaneously. In addition, both parasitoids induced leafminer mortality through larval-feeding and stinging. In order to identify and characterize endophytic fungi that could possibly be used for control of these pests, fungi were isolated from the aboveground parts of maize, sorghum, Napier grass, Coleopteran larvae and Busseola fusca pupae. Identified fungi were evaluated endophytically in two host plants species (Phaseolus vulgaris and Vicia faba) through seed inoculation. The fungal isolates that succeeded in colonizing the host plants were all pathogenic to L. huidobrensis, causing 100% mortality within 13.2 ± 0.7-15.0 ± 0.6 days. They were also able to reduce the longevity of the progeny, the number of pupae and adult emergence and survival. In addition, results also showed that endophytically-inoculated and L. huidobrensis-infested V. faba plants had no adverse effects on parasitism rates and life history parameters of P. scabriventris and D. isaea. Bio-prospecting for fungal endophytes in P. vulgaris and V. faba seeds, followed by morphological and molecular identification revealed the presence of various species of fungal entomopathogens, including Beauveria bassiana, Epacris microphylla, Phanerochaete chrysosporium and Metarhizium anisopliae. / PhD (Environmental Sciences), North-West University, Potchefstroom Campus, 2013

An estimated 30 to 36 million people worldwide are living with the Human Immunodeficiency Virus (HIV). In 2009 about 5.7 million of the 30 to 36 million people who are infected with HIV were living in South Africa, making South Africa the country with the largest number of people infected with HIV in the world (UNGASS, 2010). Van Dyk (2008) states that HIV infection and Acquired Immunodeficiency Syndrome (AIDS) are accompanied by symptoms of psycho–social distress, but relatively little is known of the direct effect of HIV and AIDS on psychological well–being. The psychological distress is mainly due to the difficulties HIV brings to daily life and the harsh reality of the prognosis of the illness (Van Dyk, 2008). It is not clear whether people infected with HIV who are unaware of their HIV status show more psychological symptoms than people in a group not infected with HIV. The research question for the current study was therefore whether people with and without HIV infection differ in their psycho–social symptoms and strengths before they know their HIV status. Accordingly, the aim of this study was to explore the psychosocial health profiles of people with and without HIV and AIDS before they knew their infection status. A cross–sectional survey design was used for gathering psychological data. This was part of a multi–disciplinary study where the participants’ HIV status was determined after obtaining their informed consent and giving pre– and post–test counselling. This study falls in the overlap of the South African leg of the Prospective Urban and Rural Epidemiology study (PURE–SA) that investigates the health transition and chronic diseases of lifestyle in urban and rural areas (Teo, Chow, Vaz, Rangarajan, & Ysusf, 2009), and the FORT2 and 3 projects (FORT2 = Understanding and promoting psychosocial health, resilience and strengths in an African context; Fort 3 = The prevalence of levels of psychosocial health: Dynamics and relationships with biomarkers of (ill) health in the South African contexts) (Wissing, 2005, 2008) on psychological well–being and its biological correlates. All the baseline data were collected during 2005. Of the 1 025 participants who completed all of the psychological health questionnaires, 153 (14.9%) were infected with HIV and 863 were not infected with HIV (since the HIV status of nine of the participants was not known, they were not included in the study). In the urban communities 435 participants completed the psychological health questionnaires, of whom 68 (15.6%) were infected with HIV and 367 were not infected with HIV. In the rural communities, 581 participants completed the psychological health questionnaires, of whom 85 (14.6%) were infected with HIV and 496 were not infected with HIV. The validated Setswana versions of the following seven psychological health questionnaires were used: Affectometer 2 (AFM), Satisfaction With Life Scale (SWLS), Community Collective Efficacy Scale (CCES), Mental Health Continuum Short Form (MHC–SF), New General Self–efficacy Scale (NGSE), Sense of Coherence Scale (SOC) and the General Health Questionnaire (GHQ). Descriptive statistics were determined for all measures for all the participants with, and without HIV. Significant differences in psychosocial profiles among individuals with and without HIV and AIDS and also between those in the rural and urban areas were determined by means of t–tests and by a multivariate analysis of variance (MANOVA). Practical significance was determined by the size of the effects. The results for the entire group showed statistically significant differences between the two groups of participants who were infected with HIV and those not infected with HIV regarding their sense of coherence and their perspective on the community’s capacity to succeed in joint activities, but these differences were of only small practical significance. The HIV–infected participants in the urban areas displayed statistically and practically a lower sense of coherence and viewed themselves as less capable of meeting task demands in community contexts, than did the participants not infected with HIV. Though the participants not infected with HIV in the rural group had, statistically and practically, a significantly greater capacity to succeed in joint community activities than the participants infected with HIV, an interesting finding was that the participants infected with HIV experienced more positive affect than the participants not infected with HIV. The research showed that people with and without HIV infection differ in some respects in their psycho–social symptoms and strengths even before they are conscious of their HIV status. It is striking that the differences found on the psychological measures for the participants reflected a personal sense of social coherence and perspective on their community’s capacity to succeed in joint activities, which was lower in the case of participants infected with HIV, and might therefore have led to high–risk social behaviours and consequent infections. It might be that the participants with a relatively lower sense of social coherence, integration, and co–operation towards collectively achieving meaningful goals were more inclined to manifest behaviours that would lead to detrimental consequences (in this case HIV infection) for themselves and others. The higher level of positive affect in the rural group of the participants infected with HIV is still unexplained and requires further research. / Thesis (M.Sc. (Clinical Psychology))--North-West University, Potchefstroom Campus, 2011.

An estimated 30 to 36 million people worldwide are living with the Human Immunodeficiency Virus (HIV). In 2009 about 5.7 million of the 30 to 36 million people who are infected with HIV were living in South Africa, making South Africa the country with the largest number of people infected with HIV in the world (UNGASS, 2010). Van Dyk (2008) states that HIV infection and Acquired Immunodeficiency Syndrome (AIDS) are accompanied by symptoms of psycho–social distress, but relatively little is known of the direct effect of HIV and AIDS on psychological well–being. The psychological distress is mainly due to the difficulties HIV brings to daily life and the harsh reality of the prognosis of the illness (Van Dyk, 2008). It is not clear whether people infected with HIV who are unaware of their HIV status show more psychological symptoms than people in a group not infected with HIV. The research question for the current study was therefore whether people with and without HIV infection differ in their psycho–social symptoms and strengths before they know their HIV status. Accordingly, the aim of this study was to explore the psychosocial health profiles of people with and without HIV and AIDS before they knew their infection status. A cross–sectional survey design was used for gathering psychological data. This was part of a multi–disciplinary study where the participants’ HIV status was determined after obtaining their informed consent and giving pre– and post–test counselling. This study falls in the overlap of the South African leg of the Prospective Urban and Rural Epidemiology study (PURE–SA) that investigates the health transition and chronic diseases of lifestyle in urban and rural areas (Teo, Chow, Vaz, Rangarajan, & Ysusf, 2009), and the FORT2 and 3 projects (FORT2 = Understanding and promoting psychosocial health, resilience and strengths in an African context; Fort 3 = The prevalence of levels of psychosocial health: Dynamics and relationships with biomarkers of (ill) health in the South African contexts) (Wissing, 2005, 2008) on psychological well–being and its biological correlates. All the baseline data were collected during 2005. Of the 1 025 participants who completed all of the psychological health questionnaires, 153 (14.9%) were infected with HIV and 863 were not infected with HIV (since the HIV status of nine of the participants was not known, they were not included in the study). In the urban communities 435 participants completed the psychological health questionnaires, of whom 68 (15.6%) were infected with HIV and 367 were not infected with HIV. In the rural communities, 581 participants completed the psychological health questionnaires, of whom 85 (14.6%) were infected with HIV and 496 were not infected with HIV. The validated Setswana versions of the following seven psychological health questionnaires were used: Affectometer 2 (AFM), Satisfaction With Life Scale (SWLS), Community Collective Efficacy Scale (CCES), Mental Health Continuum Short Form (MHC–SF), New General Self–efficacy Scale (NGSE), Sense of Coherence Scale (SOC) and the General Health Questionnaire (GHQ). Descriptive statistics were determined for all measures for all the participants with, and without HIV. Significant differences in psychosocial profiles among individuals with and without HIV and AIDS and also between those in the rural and urban areas were determined by means of t–tests and by a multivariate analysis of variance (MANOVA). Practical significance was determined by the size of the effects. The results for the entire group showed statistically significant differences between the two groups of participants who were infected with HIV and those not infected with HIV regarding their sense of coherence and their perspective on the community’s capacity to succeed in joint activities, but these differences were of only small practical significance. The HIV–infected participants in the urban areas displayed statistically and practically a lower sense of coherence and viewed themselves as less capable of meeting task demands in community contexts, than did the participants not infected with HIV. Though the participants not infected with HIV in the rural group had, statistically and practically, a significantly greater capacity to succeed in joint community activities than the participants infected with HIV, an interesting finding was that the participants infected with HIV experienced more positive affect than the participants not infected with HIV. The research showed that people with and without HIV infection differ in some respects in their psycho–social symptoms and strengths even before they are conscious of their HIV status. It is striking that the differences found on the psychological measures for the participants reflected a personal sense of social coherence and perspective on their community’s capacity to succeed in joint activities, which was lower in the case of participants infected with HIV, and might therefore have led to high–risk social behaviours and consequent infections. It might be that the participants with a relatively lower sense of social coherence, integration, and co–operation towards collectively achieving meaningful goals were more inclined to manifest behaviours that would lead to detrimental consequences (in this case HIV infection) for themselves and others. The higher level of positive affect in the rural group of the participants infected with HIV is still unexplained and requires further research. / Thesis (M.Sc. (Clinical Psychology))--North-West University, Potchefstroom Campus, 2011.

Objective: The primary objective of this study was to investigate the perception and handling of interpersonal conflict within the gender spectrum compilation of biological sex and gender identity. Methods: The research of this study was conducted by means of a theoretical study and a quantitative empirical analysis. Literature analysis and scientific theories form the basis for the first three chapters of this study. These include the philosophies of Karl Marx and Max Weber, followed by a detailed discussion on the dynamics of conflict. The empirical analysis utilized cross-sectional survey design, with a combined convenience quota sample of employees (n=133) within the company taken. This consisted of top management, middle management and lower management. The empirical study utilized the Bem Sex Role Inventory (BSRI) and the Rahim Organisational Conflict Inventory-II (ROCI-II). The Bem Sex Role Inventory (BSRI) provided self-determining assessments of masculinity and femininity in terms of the respondent’s self-reported control of socially desirable, stereotypically masculine and feminine personality characteristics. The Rahim Organisational Conflict Inventory-II (ROCI-II) measured the present methods of conflict management specifically within an organizational environment. Results: It was noted that there was no difference in how pure biological sex (males and females) perceived and handled conflict within the workplace; but there was a difference in how the different gender identity groups (masculine males vs. feminine males and feminine females vs. masculine females) perceived and handled conflict. Conclusion: This study highlighted the conflict-dynamics within the gender-spectrum of a large South African sugar manufacturing company. Results of the study proved that the motivation towards this study was achieved in the sense that, although there were no differences in how different genders perceive and handle conflict, differences were found in the different gender identities within each of those genders. It is recommended that further research include a comparative study between two or three manufacturing companies to see how different companies within the same industry deviate from or resemble the results of this study. Further research could also determine whether there are differences, by cross-checking all four gender identity groups (masculine males, feminine males, feminine females and masculine females) using the MANOVA statistical procedure. / MCom (Labour Relations Management), North-West University, Potchefstroom Campus, 2015

Objective: The primary objective of this study was to investigate the perception and handling of interpersonal conflict within the gender spectrum compilation of biological sex and gender identity. Methods: The research of this study was conducted by means of a theoretical study and a quantitative empirical analysis. Literature analysis and scientific theories form the basis for the first three chapters of this study. These include the philosophies of Karl Marx and Max Weber, followed by a detailed discussion on the dynamics of conflict. The empirical analysis utilized cross-sectional survey design, with a combined convenience quota sample of employees (n=133) within the company taken. This consisted of top management, middle management and lower management. The empirical study utilized the Bem Sex Role Inventory (BSRI) and the Rahim Organisational Conflict Inventory-II (ROCI-II). The Bem Sex Role Inventory (BSRI) provided self-determining assessments of masculinity and femininity in terms of the respondent’s self-reported control of socially desirable, stereotypically masculine and feminine personality characteristics. The Rahim Organisational Conflict Inventory-II (ROCI-II) measured the present methods of conflict management specifically within an organizational environment. Results: It was noted that there was no difference in how pure biological sex (males and females) perceived and handled conflict within the workplace; but there was a difference in how the different gender identity groups (masculine males vs. feminine males and feminine females vs. masculine females) perceived and handled conflict. Conclusion: This study highlighted the conflict-dynamics within the gender-spectrum of a large South African sugar manufacturing company. Results of the study proved that the motivation towards this study was achieved in the sense that, although there were no differences in how different genders perceive and handle conflict, differences were found in the different gender identities within each of those genders. It is recommended that further research include a comparative study between two or three manufacturing companies to see how different companies within the same industry deviate from or resemble the results of this study. Further research could also determine whether there are differences, by cross-checking all four gender identity groups (masculine males, feminine males, feminine females and masculine females) using the MANOVA statistical procedure. / MCom (Labour Relations Management), North-West University, Potchefstroom Campus, 2015